P68616 (A33_VACCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein A33

Gene names

ORF Names:

A33R

Organism

Vaccinia virus (strain Copenhagen) (VACV) [Complete proteome]

Taxonomic identifier

10249 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Coordinates the incorporation of A36 into intracellular envelopped virion (IEV) membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles By similarity.
Subunit structure	Homodimer; disulfide-linked. Interacts with B5. Interacts (via C-terminus) with protein A36 By similarity.
Subcellular location	Host membrane; Single-pass type II membrane protein Potential. Note: Present in the enveloped virion (EV) membrane By similarity.
Sequence similarities	Belongs to the chordopoxvirinae A33 protein family.

Ontologies

Keywords
Cellular component	Host membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	host cell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Protein A33		PRO_0000099316
Regions
	Topological domain	1 – 33	33	Cytoplasmic By similarity
	Transmembrane	34 – 56	23	Helical; Potential
	Topological domain	57 – 185	129	Lumenal By similarity
	Region	98 – 185	88	C-type lectin-like domain
Amino acid modifications
	Glycosylation	125	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	135	1	N-linked (GlcNAc...); by host Potential
	Disulfide bond	62		Interchain

Sequences

	Sequence		Length	Mass (Da)
	P68616 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 201CF65C2D2076A4	FASTA	185	20,506
	10 20 30 40 50 60 MMTPENDEEQ TSVFSATVYG DKIQGKNKRK RVIGLCIRIS MVISLLSMIT MSAFLIVRLN 70 80 90 100 110 120 QCMSANEAAI TDAAVAVAAA SSTHRKVASS TTQYDHKESC NGLYYQGSCY ILHSDYQLFS 130 140 150 160 170 180 DAKANCTAES STLPNKSDVL ITWLIDYVED TWGSDGNPIT KTTSDYQDSD VSQEVRKYFC VKTMN

References

[1]	"The complete DNA sequence of vaccinia virus." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:247-266(1990) [PubMed: 2219722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Appendix to 'The complete DNA sequence of vaccinia virus'." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:517-563(1990) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35027 Genomic DNA. Translation: AAA48160.1.
PIR	H42520.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016186. C-type_lectin-like. IPR009238. Chordopox_A33R. [Graphical view]
Gene3D	G3DSA:3.10.100.10. C-type_lectin-like. 1 hit.
Pfam	PF05966. Chordopox_A33R. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A33_VACCC

Accession

Primary (citable) accession number: P68616
Secondary accession number(s): P21056

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	December 7, 2004
Last modified:	October 19, 2011
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program