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Protein

Modification methylase Phi3TI

Gene

mtbP

Organism
Bacillus phage phi3T (Bacteriophage phi-3T)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme methylates the first cytosine within the sequences GGCC and GCNGC.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi2633. M.Phi3TI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase Phi3TI (EC:2.1.1.37)
Short name:
M.Phi3TI
Alternative name(s):
Cytosine-specific methyltransferase Phi3TI
Gene namesi
Name:mtbP
OrganismiBacillus phage phi3T (Bacteriophage phi-3T)
Taxonomic identifieri10736 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
Virus hostiBacillus subtilis [TaxID: 1423]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Modification methylase Phi3TIPRO_0000087866Add
BLAST

Structurei

3D structure databases

SMRiP68586. Positions 1-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 440437SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 4 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68586-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLRVMSLF SGIGAFEAAL RNIGVDYELI GFSEIDKYAI KSYCAIHNVS
60 70 80 90 100
ETLNVGDISK AKKDNIPYFD LLTSGFPCPT FSVAGGRDGM EYKCSNCSHE
110 120 130 140 150
HLITYEDYKK GVKCPKCEAV SKAKDERGTL FFETALLAEE KKPKFVILEN
160 170 180 190 200
VKGLINSGNG QVLRIISETM NNIGYRIDLE LLNSKFFNVP QNRERVYIIG
210 220 230 240 250
IREDLVENEQ WVVGQKRNDV LSKGKKRLQE INIKSFNFKW PLQDTVTKRL
260 270 280 290 300
REILEDFVDE KYYLNEEKTK KLVEQLGTAP LQKQEVREPL MVGHVDLKGH
310 320 330 340 350
DAIKRVYSPE GLSPTLTTMG GGHREPKIAE KQKEVRAVLT PEREEKRQNG
360 370 380 390 400
RRFKENGEPA FTVNTIDRHG VAIGEYPKYK IRKLSPLECW RLQAFDDEDF
410 420 430 440
EKAFAAGISN SQLYKQAGNS ITVSVLESIF QELIHTYVNK ESE
Length:443
Mass (Da):50,510
Last modified:December 6, 2004 - v1
Checksum:i2BF02005159E9879
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401I → V (PubMed:3142766).Curated
Sequence conflicti196 – 1961V → L (PubMed:3142766).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13488 Genomic DNA. Translation: AAA32352.1.
PIRiA24465. CTBPPT.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13488 Genomic DNA. Translation: AAA32352.1.
PIRiA24465. CTBPPT.

3D structure databases

SMRiP68586. Positions 1-441.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi2633. M.Phi3TI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 4 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "DNA methyltransferase genes of Bacillus subtilis phages: comparison of their nucleotide sequences."
    Tran-Betcke A., Behrens B., Noyer-Weidner M., Trautner T.A.
    Gene 42:89-96(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequential order of target-recognizing domains in multispecific DNA-methyltransferases."
    Wilke K., Rauhut E., Noyer-Weidner M., Lauster R., Pawlek B., Behrens B., Trautner T.A.
    EMBO J. 7:2601-2609(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-91 AND 125-443.

Entry informationi

Entry nameiMTBP_BPPHT
AccessioniPrimary (citable) accession number: P68586
Secondary accession number(s): P05795
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2004
Last sequence update: December 6, 2004
Last modified: March 31, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.