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Protein

Modification methylase Phi3TI

Gene

mtbP

Organism
Bacillus phage phi3T (Bacteriophage phi-3T)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme methylates the first cytosine within the sequences GGCC and GCNGC.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi2633 M.Phi3TI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase Phi3TI (EC:2.1.1.37)
Short name:
M.Phi3TI
Alternative name(s):
Cytosine-specific methyltransferase Phi3TI
Gene namesi
Name:mtbP
OrganismiBacillus phage phi3T (Bacteriophage phi-3T)
Taxonomic identifieri10736 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeSpbetavirusunclassified Spbetalikevirus
Virus hostiBacillus subtilis [TaxID: 1423]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878661 – 443Modification methylase Phi3TIAdd BLAST443

Structurei

3D structure databases

ProteinModelPortaliP68586
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 440SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST437

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 2 hits
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 4 hits
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P68586-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLRVMSLF SGIGAFEAAL RNIGVDYELI GFSEIDKYAI KSYCAIHNVS
60 70 80 90 100
ETLNVGDISK AKKDNIPYFD LLTSGFPCPT FSVAGGRDGM EYKCSNCSHE
110 120 130 140 150
HLITYEDYKK GVKCPKCEAV SKAKDERGTL FFETALLAEE KKPKFVILEN
160 170 180 190 200
VKGLINSGNG QVLRIISETM NNIGYRIDLE LLNSKFFNVP QNRERVYIIG
210 220 230 240 250
IREDLVENEQ WVVGQKRNDV LSKGKKRLQE INIKSFNFKW PLQDTVTKRL
260 270 280 290 300
REILEDFVDE KYYLNEEKTK KLVEQLGTAP LQKQEVREPL MVGHVDLKGH
310 320 330 340 350
DAIKRVYSPE GLSPTLTTMG GGHREPKIAE KQKEVRAVLT PEREEKRQNG
360 370 380 390 400
RRFKENGEPA FTVNTIDRHG VAIGEYPKYK IRKLSPLECW RLQAFDDEDF
410 420 430 440
EKAFAAGISN SQLYKQAGNS ITVSVLESIF QELIHTYVNK ESE
Length:443
Mass (Da):50,510
Last modified:December 7, 2004 - v1
Checksum:i2BF02005159E9879
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40I → V (PubMed:3142766).Curated1
Sequence conflicti196V → L (PubMed:3142766).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13488 Genomic DNA Translation: AAA32352.1
PIRiA24465 CTBPPT

Similar proteinsi

Entry informationi

Entry nameiMTBP_BPPHT
AccessioniPrimary (citable) accession number: P68586
Secondary accession number(s): P05795
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: April 25, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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