P68586 (MTBP_BPPHT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase Phi3TI
Short name=M.Phi3TI
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase Phi3TI

Gene names

Name:

mtbP

Organism

Bacillus phage phi3T (Bacteriophage phi-3T)

Taxonomic identifier

10736 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambdalikevirus › unclassified Lambda-like viruses

Virus host

Bacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme methylates the first cytosine within the sequences GGCC and GCNGC.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Modification methylase Phi3TI		PRO_0000087866
Sites
	Active site	78	1	By similarity
Experimental info
	Sequence conflict	40	1	I → V Ref.2
	Sequence conflict	196	1	V → L Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P68586 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 2BF02005159E9879	FASTA	443	50,510
	10 20 30 40 50 60 MSKLRVMSLF SGIGAFEAAL RNIGVDYELI GFSEIDKYAI KSYCAIHNVS ETLNVGDISK 70 80 90 100 110 120 AKKDNIPYFD LLTSGFPCPT FSVAGGRDGM EYKCSNCSHE HLITYEDYKK GVKCPKCEAV 130 140 150 160 170 180 SKAKDERGTL FFETALLAEE KKPKFVILEN VKGLINSGNG QVLRIISETM NNIGYRIDLE 190 200 210 220 230 240 LLNSKFFNVP QNRERVYIIG IREDLVENEQ WVVGQKRNDV LSKGKKRLQE INIKSFNFKW 250 260 270 280 290 300 PLQDTVTKRL REILEDFVDE KYYLNEEKTK KLVEQLGTAP LQKQEVREPL MVGHVDLKGH 310 320 330 340 350 360 DAIKRVYSPE GLSPTLTTMG GGHREPKIAE KQKEVRAVLT PEREEKRQNG RRFKENGEPA 370 380 390 400 410 420 FTVNTIDRHG VAIGEYPKYK IRKLSPLECW RLQAFDDEDF EKAFAAGISN SQLYKQAGNS 430 440 ITVSVLESIF QELIHTYVNK ESE

References

[1]	"DNA methyltransferase genes of Bacillus subtilis phages: comparison of their nucleotide sequences." Tran-Betcke A., Behrens B., Noyer-Weidner M., Trautner T.A. Gene 42:89-96(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequential order of target-recognizing domains in multispecific DNA-methyltransferases." Wilke K., Rauhut E., Noyer-Weidner M., Lauster R., Pawlek B., Behrens B., Trautner T.A. EMBO J. 7:2601-2609(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-91 AND 125-443.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M13488 Genomic DNA. Translation: AAA32352.1.
PIR	CTBPPT. A24465.
3D structure databases
ProteinModelPortal	P68586.
SMR	P68586. Positions 1-441.
ModBase	Search...
Protein family/group databases
REBASE	2633. M.Phi3TI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 2 hits. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTBP_BPPHT

Accession

Primary (citable) accession number: P68586
Secondary accession number(s): P05795

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	December 7, 2004
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program