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P68586

- MTBP_BPPHT

UniProt

P68586 - MTBP_BPPHT

Protein

Modification methylase Phi3TI

Gene

mtbP

Organism
Bacillus phage phi3T (Bacteriophage phi-3T)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    This enzyme methylates the first cytosine within the sequences GGCC and GCNGC.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei78 – 781PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi2633. M.Phi3TI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase Phi3TI (EC:2.1.1.37)
    Short name:
    M.Phi3TI
    Alternative name(s):
    Cytosine-specific methyltransferase Phi3TI
    Gene namesi
    Name:mtbP
    OrganismiBacillus phage phi3T (Bacteriophage phi-3T)
    Taxonomic identifieri10736 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
    Virus hostiBacillus subtilis [TaxID: 1423]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443Modification methylase Phi3TIPRO_0000087866Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP68586.
    SMRiP68586. Positions 1-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 440437SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 2 hits.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 4 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P68586-1 [UniParc]FASTAAdd to Basket

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    MSKLRVMSLF SGIGAFEAAL RNIGVDYELI GFSEIDKYAI KSYCAIHNVS    50
    ETLNVGDISK AKKDNIPYFD LLTSGFPCPT FSVAGGRDGM EYKCSNCSHE 100
    HLITYEDYKK GVKCPKCEAV SKAKDERGTL FFETALLAEE KKPKFVILEN 150
    VKGLINSGNG QVLRIISETM NNIGYRIDLE LLNSKFFNVP QNRERVYIIG 200
    IREDLVENEQ WVVGQKRNDV LSKGKKRLQE INIKSFNFKW PLQDTVTKRL 250
    REILEDFVDE KYYLNEEKTK KLVEQLGTAP LQKQEVREPL MVGHVDLKGH 300
    DAIKRVYSPE GLSPTLTTMG GGHREPKIAE KQKEVRAVLT PEREEKRQNG 350
    RRFKENGEPA FTVNTIDRHG VAIGEYPKYK IRKLSPLECW RLQAFDDEDF 400
    EKAFAAGISN SQLYKQAGNS ITVSVLESIF QELIHTYVNK ESE 443
    Length:443
    Mass (Da):50,510
    Last modified:December 7, 2004 - v1
    Checksum:i2BF02005159E9879
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti40 – 401I → V(PubMed:3142766)Curated
    Sequence conflicti196 – 1961V → L(PubMed:3142766)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13488 Genomic DNA. Translation: AAA32352.1.
    PIRiA24465. CTBPPT.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13488 Genomic DNA. Translation: AAA32352.1 .
    PIRi A24465. CTBPPT.

    3D structure databases

    ProteinModelPortali P68586.
    SMRi P68586. Positions 1-441.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 2633. M.Phi3TI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 2 hits.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 4 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA methyltransferase genes of Bacillus subtilis phages: comparison of their nucleotide sequences."
      Tran-Betcke A., Behrens B., Noyer-Weidner M., Trautner T.A.
      Gene 42:89-96(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequential order of target-recognizing domains in multispecific DNA-methyltransferases."
      Wilke K., Rauhut E., Noyer-Weidner M., Lauster R., Pawlek B., Behrens B., Trautner T.A.
      EMBO J. 7:2601-2609(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-91 AND 125-443.

    Entry informationi

    Entry nameiMTBP_BPPHT
    AccessioniPrimary (citable) accession number: P68586
    Secondary accession number(s): P05795
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3