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Protein

Ribosomal RNA small subunit methyltransferase J

Gene

rsmJ

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the guanosine in position 1516 of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(1516) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1516) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOO157:YHIQ-MONOMER.
BRENDAi2.1.1.242. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase JUniRule annotation (EC:2.1.1.242UniRule annotation)
Alternative name(s):
16S rRNA m2G1516 methyltransferaseUniRule annotation
rRNA (guanine-N(2)-)-methyltransferaseUniRule annotation
Gene namesi
Name:rsmJUniRule annotation
Synonyms:yhiQ
Ordered Locus Names:Z4897, ECs4369
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002120651 – 250Ribosomal RNA small subunit methyltransferase JAdd BLAST250

Interactioni

Protein-protein interaction databases

STRINGi155864.Z4897.

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi15 – 22Combined sources8
Beta strandi32 – 37Combined sources6
Beta strandi42 – 46Combined sources5
Helixi50 – 52Combined sources3
Beta strandi59 – 61Combined sources3
Helixi62 – 70Combined sources9
Helixi73 – 75Combined sources3
Helixi77 – 81Combined sources5
Beta strandi92 – 94Combined sources3
Helixi101 – 109Combined sources9
Beta strandi113 – 116Combined sources4
Helixi120 – 134Combined sources15
Turni137 – 139Combined sources3
Helixi140 – 146Combined sources7
Beta strandi147 – 150Combined sources4
Turni154 – 156Combined sources3
Helixi157 – 160Combined sources4
Beta strandi166 – 170Combined sources5
Helixi185 – 193Combined sources9
Helixi200 – 203Combined sources4
Helixi204 – 210Combined sources7
Beta strandi212 – 220Combined sources9
Helixi226 – 228Combined sources3
Beta strandi234 – 236Combined sources3
Beta strandi238 – 246Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PGXX-ray2.00A1-250[»]
ProteinModelPortaliP68568.
SMRiP68568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 102S-adenosyl-L-methionine bindingUniRule annotation2
Regioni117 – 118S-adenosyl-L-methionine bindingUniRule annotation2
Regioni153 – 154S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RsmJ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105FFN. Bacteria.
ENOG410XPRJ. LUCA.
HOGENOMiHOG000218236.
KOiK15984.
OMAiSSRYDIY.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01523. 16SrRNA_methyltr_J. 1 hit.
InterProiIPR007536. 16SrRNA_methylTrfase_J.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04445. SAM_MT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P68568-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKICLIDETG TGDGALSVLA ARWGLEHDED NLMALVLTPE HLELRKRDEP
60 70 80 90 100
KLGGIFVDFV GGAMAHRRKF GGGRGEAVAK AVGIKGDYLP DVVDATAGLG
110 120 130 140 150
RDAFVLASVG CRVRMLERNP VVAALLDDGL ARGYADAEIG GWLQERLQLI
160 170 180 190 200
HASSLTALTD ITPRPQVVYL DPMFPHKQKS ALVKKEMRVF QSLVGPDLDA
210 220 230 240 250
DGLLEPARLL ATKRVVVKRP DYAPPLANVA TPNAVVTKGH RFDIYAGTPV
Length:250
Mass (Da):26,949
Last modified:December 7, 2004 - v1
Checksum:i628C4329BAC1C97C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58629.1.
BA000007 Genomic DNA. Translation: BAB37792.1.
PIRiA86021.
A98175.
RefSeqiNP_312396.1. NC_002695.1.
WP_000686620.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG58629; AAG58629; Z4897.
BAB37792; BAB37792; BAB37792.
GeneIDi915773.
KEGGiece:Z4897.
ecs:ECs4369.
PATRICi18358309. VBIEscCol44059_4321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58629.1.
BA000007 Genomic DNA. Translation: BAB37792.1.
PIRiA86021.
A98175.
RefSeqiNP_312396.1. NC_002695.1.
WP_000686620.1. NZ_LPWC02000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PGXX-ray2.00A1-250[»]
ProteinModelPortaliP68568.
SMRiP68568.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z4897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58629; AAG58629; Z4897.
BAB37792; BAB37792; BAB37792.
GeneIDi915773.
KEGGiece:Z4897.
ecs:ECs4369.
PATRICi18358309. VBIEscCol44059_4321.

Phylogenomic databases

eggNOGiENOG4105FFN. Bacteria.
ENOG410XPRJ. LUCA.
HOGENOMiHOG000218236.
KOiK15984.
OMAiSSRYDIY.

Enzyme and pathway databases

BioCyciECOO157:YHIQ-MONOMER.
BRENDAi2.1.1.242. 2026.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01523. 16SrRNA_methyltr_J. 1 hit.
InterProiIPR007536. 16SrRNA_methylTrfase_J.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04445. SAM_MT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMJ_ECO57
AccessioniPrimary (citable) accession number: P68568
Secondary accession number(s): P37633, P76707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.