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Protein

Ribosomal RNA small subunit methyltransferase J

Gene

rsmJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the guanosine in position 1516 of 16S rRNA.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(1516) in 16S rRNA = S-adenosyl-L-homocysteine + N2-methylguanine(1516) in 16S rRNA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

  • 16S rRNA (guanine(1516)-N(2))-methyltransferase activity Source: EcoCyc
  • rRNA (guanine-N2-)-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
  • rRNA methylation Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG12233-MONOMER
MetaCyc:EG12233-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase JUniRule annotation (EC:2.1.1.242UniRule annotation)
Alternative name(s):
16S rRNA m2G1516 methyltransferaseUniRule annotation
rRNA (guanine-N(2)-)-methyltransferaseUniRule annotation
Gene namesi
Name:rsmJUniRule annotation
Synonyms:yhiQ
Ordered Locus Names:b3497, JW5672
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12233 rsmJ

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutants exhibit a cold-sensitive phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002120641 – 250Ribosomal RNA small subunit methyltransferase JAdd BLAST250

Proteomic databases

PaxDbiP68567
PRIDEiP68567

Interactioni

Protein-protein interaction databases

BioGridi4259385, 12 interactors
IntActiP68567, 7 interactors
STRINGi316385.ECDH10B_3673

Structurei

3D structure databases

ProteinModelPortaliP68567
SMRiP68567
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68567

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 102S-adenosyl-L-methionine bindingUniRule annotation2
Regioni117 – 118S-adenosyl-L-methionine bindingUniRule annotation2
Regioni153 – 154S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RsmJ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105FFN Bacteria
ENOG410XPRJ LUCA
HOGENOMiHOG000218236
InParanoidiP68567
KOiK15984
OMAiSRYDIYP
PhylomeDBiP68567

Family and domain databases

HAMAPiMF_01523 16SrRNA_methyltr_J, 1 hit
InterProiView protein in InterPro
IPR007536 16SrRNA_methylTrfase_J
IPR029063 SAM-dependent_MTases
PANTHERiPTHR36112 PTHR36112, 1 hit
PfamiView protein in Pfam
PF04445 SAM_MT, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

P68567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKICLIDETG TGDGALSVLA ARWGLEHDED NLMALVLTPE HLELRKRDEP
60 70 80 90 100
KLGGIFVDFV GGAMAHRRKF GGGRGEAVAK AVGIKGDYLP DVVDATAGLG
110 120 130 140 150
RDAFVLASVG CRVRMLERNP VVAALLDDGL ARGYADAEIG GWLQERLQLI
160 170 180 190 200
HASSLTALTD ITPRPQVVYL DPMFPHKQKS ALVKKEMRVF QSLVGPDLDA
210 220 230 240 250
DGLLEPARLL ATKRVVVKRP DYAPPLANVA TPNAVVTKGH RFDIYAGTPV
Length:250
Mass (Da):26,949
Last modified:December 7, 2004 - v1
Checksum:i628C4329BAC1C97C
GO

Sequence cautioni

The sequence AAB18473 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA Translation: AAB18473.1 Different initiation.
U00096 Genomic DNA Translation: AAC76522.1
AP009048 Genomic DNA Translation: BAE77797.1
PIRiD65147
RefSeqiNP_417954.1, NC_000913.3
WP_000686620.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76522; AAC76522; b3497
BAE77797; BAE77797; BAE77797
GeneIDi948005
KEGGiecj:JW5672
eco:b3497
PATRICifig|511145.12.peg.3599

Similar proteinsi

Entry informationi

Entry nameiRSMJ_ECOLI
AccessioniPrimary (citable) accession number: P68567
Secondary accession number(s): P37633, P76707, Q2M7F9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health