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Protein

Ribosomal RNA small subunit methyltransferase J

Gene

rsmJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the guanosine in position 1516 of 16S rRNA.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(1516) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1516) in 16S rRNA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

  • 16S rRNA (guanine(1516)-N(2))-methyltransferase activity Source: EcoCyc
  • rRNA (guanine-N2-)-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
  • rRNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG12233-MONOMER.
ECOL316407:JW5672-MONOMER.
MetaCyc:EG12233-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase JUniRule annotation (EC:2.1.1.242UniRule annotation)
Alternative name(s):
16S rRNA m2G1516 methyltransferaseUniRule annotation
rRNA (guanine-N(2)-)-methyltransferaseUniRule annotation
Gene namesi
Name:rsmJUniRule annotation
Synonyms:yhiQ
Ordered Locus Names:b3497, JW5672
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12233. rsmJ.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutants exhibit a cold-sensitive phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002120641 – 250Ribosomal RNA small subunit methyltransferase JAdd BLAST250

Proteomic databases

PaxDbiP68567.
PRIDEiP68567.

Interactioni

Protein-protein interaction databases

BioGridi4259385. 5 interactors.
IntActiP68567. 7 interactors.
STRINGi511145.b3497.

Structurei

3D structure databases

ProteinModelPortaliP68567.
SMRiP68567.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68567.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 102S-adenosyl-L-methionine bindingUniRule annotation2
Regioni117 – 118S-adenosyl-L-methionine bindingUniRule annotation2
Regioni153 – 154S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RsmJ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105FFN. Bacteria.
ENOG410XPRJ. LUCA.
HOGENOMiHOG000218236.
InParanoidiP68567.
KOiK15984.
OMAiSSRYDIY.
PhylomeDBiP68567.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01523. 16SrRNA_methyltr_J. 1 hit.
InterProiIPR007536. 16SrRNA_methylTrfase_J.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04445. SAM_MT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P68567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKICLIDETG TGDGALSVLA ARWGLEHDED NLMALVLTPE HLELRKRDEP
60 70 80 90 100
KLGGIFVDFV GGAMAHRRKF GGGRGEAVAK AVGIKGDYLP DVVDATAGLG
110 120 130 140 150
RDAFVLASVG CRVRMLERNP VVAALLDDGL ARGYADAEIG GWLQERLQLI
160 170 180 190 200
HASSLTALTD ITPRPQVVYL DPMFPHKQKS ALVKKEMRVF QSLVGPDLDA
210 220 230 240 250
DGLLEPARLL ATKRVVVKRP DYAPPLANVA TPNAVVTKGH RFDIYAGTPV
Length:250
Mass (Da):26,949
Last modified:December 7, 2004 - v1
Checksum:i628C4329BAC1C97C
GO

Sequence cautioni

The sequence AAB18473 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18473.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76522.1.
AP009048 Genomic DNA. Translation: BAE77797.1.
PIRiD65147.
RefSeqiNP_417954.1. NC_000913.3.
WP_000686620.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76522; AAC76522; b3497.
BAE77797; BAE77797; BAE77797.
GeneIDi948005.
KEGGiecj:JW5672.
eco:b3497.
PATRICi32122444. VBIEscCol129921_3599.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18473.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76522.1.
AP009048 Genomic DNA. Translation: BAE77797.1.
PIRiD65147.
RefSeqiNP_417954.1. NC_000913.3.
WP_000686620.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP68567.
SMRiP68567.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259385. 5 interactors.
IntActiP68567. 7 interactors.
STRINGi511145.b3497.

Proteomic databases

PaxDbiP68567.
PRIDEiP68567.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76522; AAC76522; b3497.
BAE77797; BAE77797; BAE77797.
GeneIDi948005.
KEGGiecj:JW5672.
eco:b3497.
PATRICi32122444. VBIEscCol129921_3599.

Organism-specific databases

EchoBASEiEB2145.
EcoGeneiEG12233. rsmJ.

Phylogenomic databases

eggNOGiENOG4105FFN. Bacteria.
ENOG410XPRJ. LUCA.
HOGENOMiHOG000218236.
InParanoidiP68567.
KOiK15984.
OMAiSSRYDIY.
PhylomeDBiP68567.

Enzyme and pathway databases

BioCyciEcoCyc:EG12233-MONOMER.
ECOL316407:JW5672-MONOMER.
MetaCyc:EG12233-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP68567.
PROiP68567.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01523. 16SrRNA_methyltr_J. 1 hit.
InterProiIPR007536. 16SrRNA_methylTrfase_J.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04445. SAM_MT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMJ_ECOLI
AccessioniPrimary (citable) accession number: P68567
Secondary accession number(s): P37633, P76707, Q2M7F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.