P68567 (RSMJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase J
EC=2.1.1.242

Alternative name(s):
16S rRNA m2G1516 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase

Gene names

Name:	rsmJ
Synonyms:	yhiQ
Ordered Locus Names:	b3497, JW5672

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1516 of 16S rRNA. Ref.4
Catalytic activity	S-adenosyl-L-methionine + guanine(1516) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1516) in 16S rRNA. Ref.4
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01523.
Disruption phenotype	Mutants exhibit a cold-sensitive phenotype. Ref.4
Sequence similarities	Belongs to the methyltransferase superfamily. RsmJ family.
Sequence caution	The sequence AAB18473.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA base methylation Inferred from mutant phenotype Ref.4. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	16S rRNA (guanine(1516)-N(2))-methyltransferase activity Inferred from direct assay Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Ribosomal RNA small subunit methyltransferase J HAMAP-Rule MF_01523		PRO_0000212064
Regions
	Region	101 – 102	2	S-adenosyl-L-methionine binding By similarity
	Region	117 – 118	2	S-adenosyl-L-methionine binding By similarity
	Region	153 – 154	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	171	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68567 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 628C4329BAC1C97C	FASTA	250	26,949
	10 20 30 40 50 60 MKICLIDETG TGDGALSVLA ARWGLEHDED NLMALVLTPE HLELRKRDEP KLGGIFVDFV 70 80 90 100 110 120 GGAMAHRRKF GGGRGEAVAK AVGIKGDYLP DVVDATAGLG RDAFVLASVG CRVRMLERNP 130 140 150 160 170 180 VVAALLDDGL ARGYADAEIG GWLQERLQLI HASSLTALTD ITPRPQVVYL DPMFPHKQKS 190 200 210 220 230 240 ALVKKEMRVF QSLVGPDLDA DGLLEPARLL ATKRVVVKRP DYAPPLANVA TPNAVVTKGH 250 RFDIYAGTPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"YhiQ is RsmJ, the methyltransferase responsible for methylation of G1516 in 16S rRNA of E. coli." Basturea G.N., Dague D.R., Deutscher M.P., Rudd K.E. J. Mol. Biol. 415:16-21(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00039 Genomic DNA. Translation: AAB18473.1. Different initiation. U00096 Genomic DNA. Translation: AAC76522.1. AP009048 Genomic DNA. Translation: BAE77797.1.
PIR	D65147.
RefSeq	NP_417954.1. NC_000913.2. YP_491938.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P68567.
SMR	P68567. Positions 1-250.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b3497.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76522; AAC76522; b3497. BAE77797; BAE77797; BAE77797.
GeneID	12930391. 948005.
KEGG	ecj:Y75_p3680. eco:b3497.
PATRIC	32122444. VBIEscCol129921_3599.
Organism-specific databases
EchoBASE	EB2145.
EcoGene	EG12233. rsmJ.
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HOG000218236.
KO	K15984.
OMA	GQMIAKA.
ProtClustDB	PRK10742.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12233-MONOMER. ECOL316407:JW5672-MONOMER. MetaCyc:EG12233-MONOMER.
Gene expression databases
Genevestigator	P68567.
Family and domain databases
HAMAP	MF_01523. 16SrRNA_methyltr_J.
InterPro	IPR007536. 16SrRNA_methylTrfase_J. [Graphical view]
Pfam	PF04445. SAM_MT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
EvolutionaryTrace	P68567.

Entry information

Entry name

RSMJ_ECOLI

Accession

Primary (citable) accession number: P68567
Secondary accession number(s): P37633, P76707, Q2M7F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	December 7, 2004
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program