P68563 (KITH_VACCW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	TK
Ordered Locus Names:	VACWR094
ORF Names:	J2R

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) [Reference proteome]

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis By similarity.
Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Subunit structure	Homotetramer By similarity. Two molecules of substrate bind to each enzyme tetramer.
Sequence similarities	Belongs to the thymidine kinase family.
Biophysicochemical properties	Kinetic parameters: KM=25 µM for 2'deoxythymidine Ref.5

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Thymidine kinase		PRO_0000174940
Regions
	Nucleotide binding	11 – 18	8	ATP By similarity
	Region	157 – 161	5	Substrate binding By similarity
Sites
	Active site	83	1	Proton acceptor Potential
	Metal binding	138	1	Zinc By similarity
	Metal binding	141	1	Zinc By similarity
	Metal binding	170	1	Zinc By similarity
	Metal binding	173	1	Zinc By similarity
	Binding site	113	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68563 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 57E3595EBE6F3C54	FASTA	177	20,100
	10 20 30 40 50 60 MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL WTHDKNNFEA 70 80 90 100 110 120 LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE GKIVIVAALD GTFQRKPFNN 130 140 150 160 170 ILNLIPLSEM VVKLTAVCMK CFKEASFSKR LGEETEIEII GGNDMYQSVC RKCYIDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome." Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E. Nucleic Acids Res. 13:985-998(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations." Weir J.P., Moss B. J. Virol. 46:530-537(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Fine structure analysis and nucleotide sequence of the vaccinia virus thymidine kinase gene." Hruby D.E., Maki R.A., Miller D.B., Ball L.A. Proc. Natl. Acad. Sci. U.S.A. 80:3411-3415(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Biophysical characterization of vaccinia virus thymidine kinase substrate utilization." Smith R.F., Freyer M.W., Lewis E.A. J. Virol. Methods 142:151-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01978 Genomic DNA. Translation: CAA26016.1. AY243312 Genomic DNA. Translation: AAO89373.1.
PIR	KIVZ. A00609.
RefSeq	YP_232976.1. NC_006998.1.
3D structure databases
ProteinModelPortal	P68563.
SMR	P68563. Positions 4-175.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-131369.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3707550.
Phylogenomic databases
ProtClustDB	CLSP2509781.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1075034.

Entry information

Entry name

KITH_VACCW

Accession

Primary (citable) accession number: P68563
Secondary accession number(s): P03297, Q76ZT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program