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P68563

- KITH_VACCW

UniProt

P68563 - KITH_VACCW

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Protein

Thymidine kinase

Gene

TK

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis (By similarity).By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Kineticsi

  1. KM=25 µM for 2'deoxythymidine1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei83 – 831Proton acceptorSequence Analysis
Binding sitei113 – 1131Substrate; via amide nitrogenBy similarity
Metal bindingi138 – 1381ZincBy similarity
Metal bindingi141 – 1411ZincBy similarity
Metal bindingi170 – 1701ZincBy similarity
Metal bindingi173 – 1731ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 188ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
Ordered Locus Names:VACWR094
ORF Names:J2R
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000000344: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 177177Thymidine kinasePRO_0000174940Add
BLAST

Interactioni

Subunit structurei

Homotetramer (By similarity). Two molecules of substrate bind to each enzyme tetramer.By similarity

Protein-protein interaction databases

MINTiMINT-131369.

Structurei

3D structure databases

ProteinModelPortaliP68563.
SMRiP68563. Positions 4-175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1615Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68563 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL
60 70 80 90 100
WTHDKNNFEA LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE
110 120 130 140 150
GKIVIVAALD GTFQRKPFNN ILNLIPLSEM VVKLTAVCMK CFKEASFSKR
160 170
LGEETEIEII GGNDMYQSVC RKCYIDS
Length:177
Mass (Da):20,100
Last modified:July 21, 1986 - v1
Checksum:i57E3595EBE6F3C54
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01978 Genomic DNA. Translation: CAA26016.1.
AY243312 Genomic DNA. Translation: AAO89373.1.
PIRiA00609. KIVZ.
RefSeqiYP_232976.1. NC_006998.1.

Genome annotation databases

GeneIDi3707550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01978 Genomic DNA. Translation: CAA26016.1 .
AY243312 Genomic DNA. Translation: AAO89373.1 .
PIRi A00609. KIVZ.
RefSeqi YP_232976.1. NC_006998.1.

3D structure databases

ProteinModelPortali P68563.
SMRi P68563. Positions 4-175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-131369.

Chemistry

ChEMBLi CHEMBL1075034.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3707550.

Family and domain databases

InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome."
    Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E.
    Nucleic Acids Res. 13:985-998(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations."
    Weir J.P., Moss B.
    J. Virol. 46:530-537(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Fine structure analysis and nucleotide sequence of the vaccinia virus thymidine kinase gene."
    Hruby D.E., Maki R.A., Miller D.B., Ball L.A.
    Proc. Natl. Acad. Sci. U.S.A. 80:3411-3415(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
    Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Biophysical characterization of vaccinia virus thymidine kinase substrate utilization."
    Smith R.F., Freyer M.W., Lewis E.A.
    J. Virol. Methods 142:151-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiKITH_VACCW
AccessioniPrimary (citable) accession number: P68563
Secondary accession number(s): P03297, Q76ZT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 1, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3