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Protein

Thymidine kinase

Gene

TK

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis (By similarity).By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Kineticsi

  1. KM=25 µM for 2'deoxythymidine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei83Proton acceptorSequence analysis1
    Binding sitei113Substrate; via amide nitrogenBy similarity1
    Metal bindingi138ZincBy similarity1
    Metal bindingi141ZincBy similarity1
    Metal bindingi170ZincBy similarity1
    Metal bindingi173ZincBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi11 – 18ATPBy similarity8

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionKinase, Transferase
    Biological processDNA synthesis
    LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    Name:TK
    Ordered Locus Names:VACWR094
    ORF Names:J2R
    OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
    Taxonomic identifieri10254 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    Proteomesi
    • UP000000344 Componenti: Genome

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL1075034.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001749401 – 177Thymidine kinaseAdd BLAST177

    Interactioni

    Subunit structurei

    Homotetramer (By similarity). Two molecules of substrate bind to each enzyme tetramer.By similarity

    Protein-protein interaction databases

    MINTiMINT-131369.

    Structurei

    3D structure databases

    ProteinModelPortaliP68563.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni157 – 161Substrate bindingBy similarity5

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Phylogenomic databases

    KOiK00857.
    OrthoDBiVOG090000XT.

    Family and domain databases

    InterProiView protein in InterPro
    IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiView protein in Pfam
    PF00265. TK. 1 hit.
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiView protein in PROSITE
    PS00603. TK_CELLULAR_TYPE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P68563-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL
    60 70 80 90 100
    WTHDKNNFEA LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE
    110 120 130 140 150
    GKIVIVAALD GTFQRKPFNN ILNLIPLSEM VVKLTAVCMK CFKEASFSKR
    160 170
    LGEETEIEII GGNDMYQSVC RKCYIDS
    Length:177
    Mass (Da):20,100
    Last modified:July 21, 1986 - v1
    Checksum:i57E3595EBE6F3C54
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X01978 Genomic DNA. Translation: CAA26016.1.
    AY243312 Genomic DNA. Translation: AAO89373.1.
    PIRiA00609. KIVZ.
    RefSeqiYP_232976.1. NC_006998.1.

    Genome annotation databases

    GeneIDi3707550.
    KEGGivg:3707550.

    Similar proteinsi

    Entry informationi

    Entry nameiKITH_VACCW
    AccessioniPrimary (citable) accession number: P68563
    Secondary accession number(s): P03297, Q76ZT2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 25, 2017
    This is version 56 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families