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P68563

- KITH_VACCW

UniProt

P68563 - KITH_VACCW

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Protein
Thymidine kinase
Gene
TK, VACWR094, J2R
Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis By similarity.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Kineticsi

  1. KM=25 µM for 2'deoxythymidine1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei83 – 831Proton acceptor Reviewed prediction
Binding sitei113 – 1131Substrate; via amide nitrogen By similarity
Metal bindingi138 – 1381Zinc By similarity
Metal bindingi141 – 1411Zinc By similarity
Metal bindingi170 – 1701Zinc By similarity
Metal bindingi173 – 1731Zinc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 188ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
Ordered Locus Names:VACWR094
ORF Names:J2R
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000000344: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 177177Thymidine kinase
PRO_0000174940Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity. Two molecules of substrate bind to each enzyme tetramer.

Protein-protein interaction databases

MINTiMINT-131369.

Structurei

3D structure databases

ProteinModelPortaliP68563.
SMRiP68563. Positions 4-175.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1615Substrate binding By similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68563-1 [UniParc]FASTAAdd to Basket

« Hide

MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL    50
WTHDKNNFEA LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE 100
GKIVIVAALD GTFQRKPFNN ILNLIPLSEM VVKLTAVCMK CFKEASFSKR 150
LGEETEIEII GGNDMYQSVC RKCYIDS 177
Length:177
Mass (Da):20,100
Last modified:July 21, 1986 - v1
Checksum:i57E3595EBE6F3C54
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01978 Genomic DNA. Translation: CAA26016.1.
AY243312 Genomic DNA. Translation: AAO89373.1.
PIRiA00609. KIVZ.
RefSeqiYP_232976.1. NC_006998.1.

Genome annotation databases

GeneIDi3707550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01978 Genomic DNA. Translation: CAA26016.1 .
AY243312 Genomic DNA. Translation: AAO89373.1 .
PIRi A00609. KIVZ.
RefSeqi YP_232976.1. NC_006998.1.

3D structure databases

ProteinModelPortali P68563.
SMRi P68563. Positions 4-175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-131369.

Chemistry

ChEMBLi CHEMBL1075034.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3707550.

Family and domain databases

InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome."
    Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E.
    Nucleic Acids Res. 13:985-998(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations."
    Weir J.P., Moss B.
    J. Virol. 46:530-537(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Fine structure analysis and nucleotide sequence of the vaccinia virus thymidine kinase gene."
    Hruby D.E., Maki R.A., Miller D.B., Ball L.A.
    Proc. Natl. Acad. Sci. U.S.A. 80:3411-3415(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
    Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Biophysical characterization of vaccinia virus thymidine kinase substrate utilization."
    Smith R.F., Freyer M.W., Lewis E.A.
    J. Virol. Methods 142:151-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiKITH_VACCW
AccessioniPrimary (citable) accession number: P68563
Secondary accession number(s): P03297, Q76ZT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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