Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidine kinase

Gene

TK

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis (By similarity).By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Kineticsi

  1. KM=25 µM for 2'deoxythymidine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei83Proton acceptorSequence analysis1
    Binding sitei113Substrate; via amide nitrogenBy similarity1
    Metal bindingi138ZincBy similarity1
    Metal bindingi141ZincBy similarity1
    Metal bindingi170ZincBy similarity1
    Metal bindingi173ZincBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi11 – 18ATPBy similarity8

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionKinase, Transferase
    Biological processDNA synthesis
    LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    Name:TK
    Ordered Locus Names:VACWR094
    ORF Names:J2R
    OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
    Taxonomic identifieri10254 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    Proteomesi
    • UP000000344 Componenti: Genome

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL1075034

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001749401 – 177Thymidine kinaseAdd BLAST177

    Interactioni

    Subunit structurei

    Homotetramer (By similarity). Two molecules of substrate bind to each enzyme tetramer.By similarity

    Protein-protein interaction databases

    MINTiP68563

    Structurei

    3D structure databases

    ProteinModelPortaliP68563
    SMRiP68563
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni157 – 161Substrate bindingBy similarity5

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Phylogenomic databases

    KOiK00857
    OrthoDBiVOG090000XT

    Family and domain databases

    InterProiView protein in InterPro
    IPR027417 P-loop_NTPase
    IPR001267 Thymidine_kinase
    IPR020633 Thymidine_kinase_CS
    PANTHERiPTHR11441 PTHR11441, 1 hit
    PfamiView protein in Pfam
    PF00265 TK, 1 hit
    PIRSFiPIRSF035805 TK_cell, 1 hit
    SUPFAMiSSF52540 SSF52540, 1 hit
    PROSITEiView protein in PROSITE
    PS00603 TK_CELLULAR_TYPE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P68563-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL
    60 70 80 90 100
    WTHDKNNFEA LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE
    110 120 130 140 150
    GKIVIVAALD GTFQRKPFNN ILNLIPLSEM VVKLTAVCMK CFKEASFSKR
    160 170
    LGEETEIEII GGNDMYQSVC RKCYIDS
    Length:177
    Mass (Da):20,100
    Last modified:July 21, 1986 - v1
    Checksum:i57E3595EBE6F3C54
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X01978 Genomic DNA Translation: CAA26016.1
    AY243312 Genomic DNA Translation: AAO89373.1
    PIRiA00609 KIVZ
    RefSeqiYP_232976.1, NC_006998.1

    Genome annotation databases

    GeneIDi3707550
    KEGGivg:3707550

    Similar proteinsi

    Entry informationi

    Entry nameiKITH_VACCW
    AccessioniPrimary (citable) accession number: P68563
    Secondary accession number(s): P03297, Q76ZT2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: February 28, 2018
    This is version 58 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health