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P68563 (KITH_VACCW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase

EC=2.7.1.21
Gene names
Name:TK
Ordered Locus Names:VACWR094
ORF Names:J2R
OrganismVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) [Reference proteome]
Taxonomic identifier10254 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length177 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis By similarity.

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Subunit structure

Homotetramer By similarity. Two molecules of substrate bind to each enzyme tetramer.

Sequence similarities

Belongs to the thymidine kinase family.

Biophysicochemical properties

Kinetic parameters:

KM=25 µM for 2'deoxythymidine Ref.5

Ontologies

Keywords
   Biological processDNA synthesis
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

thymidine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 177177Thymidine kinase
PRO_0000174940

Regions

Nucleotide binding11 – 188ATP By similarity
Region157 – 1615Substrate binding By similarity

Sites

Active site831Proton acceptor Potential
Metal binding1381Zinc By similarity
Metal binding1411Zinc By similarity
Metal binding1701Zinc By similarity
Metal binding1731Zinc By similarity
Binding site1131Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P68563 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 57E3595EBE6F3C54

FASTA17720,100
        10         20         30         40         50         60 
MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL WTHDKNNFEA 

        70         80         90        100        110        120 
LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE GKIVIVAALD GTFQRKPFNN 

       130        140        150        160        170 
ILNLIPLSEM VVKLTAVCMK CFKEASFSKR LGEETEIEII GGNDMYQSVC RKCYIDS 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome."
Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E.
Nucleic Acids Res. 13:985-998(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations."
Weir J.P., Moss B.
J. Virol. 46:530-537(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Fine structure analysis and nucleotide sequence of the vaccinia virus thymidine kinase gene."
Hruby D.E., Maki R.A., Miller D.B., Ball L.A.
Proc. Natl. Acad. Sci. U.S.A. 80:3411-3415(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Biophysical characterization of vaccinia virus thymidine kinase substrate utilization."
Smith R.F., Freyer M.W., Lewis E.A.
J. Virol. Methods 142:151-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X01978 Genomic DNA. Translation: CAA26016.1.
AY243312 Genomic DNA. Translation: AAO89373.1.
PIRKIVZ. A00609.
RefSeqYP_232976.1. NC_006998.1.

3D structure databases

ProteinModelPortalP68563.
SMRP68563. Positions 4-175.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-131369.

Chemistry

ChEMBLCHEMBL1075034.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3707550.

Family and domain databases

InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKITH_VACCW
AccessionPrimary (citable) accession number: P68563
Secondary accession number(s): P03297, Q76ZT2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families