Reviewed, UniProtKB/Swiss-Prot P68540 (COX1_AEGCO)

Names and origin

Protein names

Recommended name:
    Cytochrome c oxidase subunit 1
    EC=1.9.3.1
Alternative name(s):
    Cytochrome c oxidase polypeptide I

Gene names

Name:	COX1
Synonyms:	COI, COXI

Encoded on

Mitochondrion

Organism

Aegilops columnaris (Goatgrass)

Taxonomic identifier

4493 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Aegilops

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Pathway	Energy metabolism; oxidative phosphorylation.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the heme-copper respiratory oxidase family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane
Ligand	Copper Heme Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: InterPro respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Cytochrome c oxidase subunit 1		PRO_0000183276
Regions
	Transmembrane	18 – 38	21	Potential
	Transmembrane	66 – 86	21	Potential
	Transmembrane	103 – 123	21	Potential
	Transmembrane	148 – 168	21	Potential
	Transmembrane	186 – 206	21	Potential
	Transmembrane	237 – 257	21	Potential
	Transmembrane	269 – 289	21	Potential
	Transmembrane	312 – 332	21	Potential
	Transmembrane	340 – 360	21	Potential
	Transmembrane	379 – 399	21	Potential
	Transmembrane	414 – 434	21	Potential
	Transmembrane	447 – 467	21	Potential
Sites
	Metal binding	64	1	Iron (heme A axial ligand) Probable
	Metal binding	243	1	Copper B Probable
	Metal binding	247	1	Copper B Probable
	Metal binding	292	1	Copper B Probable
	Metal binding	293	1	Copper B Probable
	Metal binding	378	1	Iron (heme A3 axial ligand) Probable
	Metal binding	380	1	Iron (heme A axial ligand) Probable
Amino acid modifications
	Cross-link	243 ↔ 247		1'-histidyl-3'-tyrosine (His-Tyr) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68540-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: ECD6E0AAB74F1DE1	FASTA	524	57,757
	10 20 30 40 50 60 MTNMVRWLFS TNHKDIGTLY FIFGAIAGVM GTCFSVLIRM ELARPGDQIL GGNHQLYNVL 70 80 90 100 110 120 ITAHAFLMIF FMVMPAMIGG FGNWFVPILI GAPDMAFPRL NNISFWLLPP SLLLLLSSAL 130 140 150 160 170 180 VEVGSGTGWT VYPPLSGITS HSGGAVDLAI FSLHLSGISS ILGSINFITT IFNMRGPGMT 190 200 210 220 230 240 MHRLPLFVWS VLVTAFLLLL SLPVLAGAIT MLLTDRNFNT TFFDPAGGGD PILYQHLFWF 250 260 270 280 290 300 FGHPEVYILI LPGFGIISHI VSTFSRKPVF GYLGMVYAMI SIGVLGFLVW AHHMFTVGLD 310 320 330 340 350 360 VDTRAYFTAA TMIIAVPTGI KIFSWIATMW GGSIQYKTPM LFAVGFIFLF TIGGLTGIVL 370 380 390 400 410 420 ANSGLDIALH DTYYVVAHFH YVLSMGAVFA LFAGFYYWVG KIFGRTYPET LGQIHFWITF 430 440 450 460 470 480 FGVNLTFFPM HFLGLSGMPR RIPDYPDAYA GWNALSSFGS YISVVGIRRF FVVVAITSSS 490 500 510 520 GKNQKCAESP WAVEQNPTTL EWLVQSPPAF HTFGELPAVK ETKS

References

[1]	"Deficiency of cox1 gene expression in a wheat having Aegilops columnaris cytoplasm results in an impaired cytochrome c oxidase activity." Ikeda T.M., Tsunewaki K. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U46764 Genomic DNA. Translation: AAA91209.1.
3D structure databases
HSSP	HSSP built from PDB template 2OCC based on UniProtKB P00396.
ModBase	Search...
Organism-specific databases
Gramene	P68540.
Enzyme and pathway databases
BRENDA	1.9.3.1. 299467.
Family and domain databases
InterPro	IPR000883. Cyt_c_oxidase_su1. IPR014241. Cyt_c_oxidase_su1_bac. [Graphical view]
Gene3D	G3DSA:1.20.210.10. COX1. 1 hit.
PANTHER	PTHR10422. COX1. 1 hit.
Pfam	PF00115. COX1. 1 hit. [Graphical view]
PRINTS	PR01165. CYCOXIDASEI.
TIGRFAMs	TIGR02891. CtaD_CoxA. 1 hit.
PROSITE	PS50855. COX1. 1 hit. PS00077. COX1_CUB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX1_AEGCO

Accession

Primary (citable) accession number: P68540
Secondary accession number(s): P08741

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	November 23, 2004
Last modified:	October 13, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)