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P68530

- COX2_BOVIN

UniProt

P68530 - COX2_BOVIN

Protein

Cytochrome c oxidase subunit 2

Gene

MT-CO2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

    Cofactori

    Copper A.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi161 – 1611Copper A1
    Metal bindingi196 – 1961Copper A1
    Metal bindingi196 – 1961Copper A2
    Metal bindingi198 – 1981Copper A2; via carbonyl oxygen
    Metal bindingi198 – 1981Magnesium; shared with chain I
    Metal bindingi200 – 2001Copper A1
    Metal bindingi200 – 2001Copper A2
    Metal bindingi204 – 2041Copper A2
    Metal bindingi207 – 2071Copper A1

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. cytochrome-c oxidase activity Source: InterPro

    GO - Biological processi

    1. electron transport chain Source: InterPro
    2. hydrogen ion transmembrane transport Source: GOC

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Metal-binding

    Protein family/group databases

    TCDBi3.D.4.7.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 2
    Alternative name(s):
    Cytochrome c oxidase polypeptide II
    Gene namesi
    Name:MT-CO2
    Synonyms:COII, COXII, MTCO2
    Encoded oniMitochondrion
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Mitochondrion

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: UniProtKB-SubCell
    3. respiratory chain complex IV Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 227227Cytochrome c oxidase subunit 2PRO_0000183517Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-formylmethionine1 Publication

    Keywords - PTMi

    Formylation

    Proteomic databases

    PaxDbiP68530.
    PRIDEiP68530.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-39024N.
    IntActiP68530. 1 interaction.

    Structurei

    Secondary structure

    1
    227
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi15 – 4632
    Helixi61 – 8828
    Beta strandi94 – 1029
    Beta strandi105 – 1106
    Beta strandi112 – 1143
    Beta strandi116 – 1205
    Helixi125 – 1273
    Turni134 – 1363
    Beta strandi138 – 1403
    Beta strandi142 – 1454
    Beta strandi150 – 15910
    Beta strandi161 – 1655
    Helixi166 – 1683
    Beta strandi170 – 1745
    Beta strandi180 – 1845
    Beta strandi190 – 1945
    Helixi204 – 2063
    Beta strandi209 – 2146
    Helixi216 – 22510

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OCCX-ray2.80B/O1-227[»]
    1OCOX-ray2.80B/O1-227[»]
    1OCRX-ray2.35B/O1-227[»]
    1OCZX-ray2.90B/O1-227[»]
    1V54X-ray1.80B/O1-227[»]
    1V55X-ray1.90B/O1-227[»]
    2DYRX-ray1.80B/O1-227[»]
    2DYSX-ray2.20B/O1-227[»]
    2EIJX-ray1.90B/O1-227[»]
    2EIKX-ray2.10B/O1-227[»]
    2EILX-ray2.10B/O1-227[»]
    2EIMX-ray2.60B/O1-227[»]
    2EINX-ray2.70B/O1-227[»]
    2OCCX-ray2.30B/O1-227[»]
    2Y69X-ray1.95B/O1-227[»]
    2YBBelectron microscopy19.00M1-227[»]
    2ZXWX-ray2.50B/O1-227[»]
    3ABKX-ray2.00B/O1-227[»]
    3ABLX-ray2.10B/O1-227[»]
    3ABMX-ray1.95B/O1-227[»]
    3AG1X-ray2.20B/O1-227[»]
    3AG2X-ray1.80B/O1-227[»]
    3AG3X-ray1.80B/O1-227[»]
    3AG4X-ray2.05B/O1-227[»]
    3ASNX-ray3.00B/O1-227[»]
    3ASOX-ray2.30B/O1-227[»]
    3WG7X-ray1.90B/O1-227[»]
    ProteinModelPortaliP68530.
    SMRiP68530. Positions 1-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP68530.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1414Mitochondrial intermembraneAdd
    BLAST
    Topological domaini46 – 5914Mitochondrial matrixAdd
    BLAST
    Topological domaini88 – 227140Mitochondrial intermembraneAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 4531Helical; Name=IAdd
    BLAST
    Transmembranei60 – 8728Helical; Name=IIAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1622.
    HOGENOMiHOG000264988.
    HOVERGENiHBG012727.
    InParanoidiP68530.
    KOiK02261.
    OMAiDHALMIV.
    OrthoDBiEOG7TJ3JX.
    TreeFamiTF344269.

    Family and domain databases

    Gene3Di1.10.287.90. 1 hit.
    2.60.40.420. 1 hit.
    InterProiIPR001505. Copper_CuA.
    IPR008972. Cupredoxin.
    IPR014222. Cyt_c_oxidase_su2.
    IPR002429. Cyt_c_oxidase_su2_C.
    IPR011759. Cyt_c_oxidase_su2_TM_dom.
    [Graphical view]
    PfamiPF00116. COX2. 1 hit.
    PF02790. COX2_TM. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 1 hit.
    SSF81464. SSF81464. 1 hit.
    TIGRFAMsiTIGR02866. CoxB. 1 hit.
    PROSITEiPS00078. COX2. 1 hit.
    PS50857. COX2_CUA. 1 hit.
    PS50999. COX2_TM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P68530-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYPMQLGFQ DATSPIMEEL LHFHDHTLMI VFLISSLVLY IISLMLTTKL    50
    THTSTMDAQE VETIWTILPA IILILIALPS LRILYMMDEI NNPSLTVKTM 100
    GHQWYWSYEY TDYEDLSFDS YMIPTSELKP GELRLLEVDN RVVLPMEMTI 150
    RMLVSSEDVL HSWAVPSLGL KTDAIPGRLN QTTLMSSRPG LYYGQCSEIC 200
    GSNHSFMPIV LELVPLKYFE KWSASML 227
    Length:227
    Mass (Da):26,021
    Last modified:July 21, 1986 - v1
    Checksum:iC562D5B39FA9771A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti58 – 581A → P in AAA31644. (PubMed:6265319)Curated
    Sequence conflicti118 – 1181F → L in AAA31644. (PubMed:6265319)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00654 Genomic DNA. Translation: CAA24000.1.
    M10544 Genomic DNA. Translation: AAA31644.1.
    AF490528 Genomic DNA. Translation: AAM08331.1.
    AF490529 Genomic DNA. Translation: AAM08344.1.
    AF493541 Genomic DNA. Translation: AAM12792.1.
    AF493542 Genomic DNA. Translation: AAM12805.1.
    PIRiB00152. OBBO2.
    RefSeqiYP_209208.1. NC_006853.1.

    Genome annotation databases

    EnsembliENSBTAT00000060549; ENSBTAP00000053151; ENSBTAG00000043556.
    GeneIDi3283880.
    KEGGibta:3283880.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00654 Genomic DNA. Translation: CAA24000.1 .
    M10544 Genomic DNA. Translation: AAA31644.1 .
    AF490528 Genomic DNA. Translation: AAM08331.1 .
    AF490529 Genomic DNA. Translation: AAM08344.1 .
    AF493541 Genomic DNA. Translation: AAM12792.1 .
    AF493542 Genomic DNA. Translation: AAM12805.1 .
    PIRi B00152. OBBO2.
    RefSeqi YP_209208.1. NC_006853.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OCC X-ray 2.80 B/O 1-227 [» ]
    1OCO X-ray 2.80 B/O 1-227 [» ]
    1OCR X-ray 2.35 B/O 1-227 [» ]
    1OCZ X-ray 2.90 B/O 1-227 [» ]
    1V54 X-ray 1.80 B/O 1-227 [» ]
    1V55 X-ray 1.90 B/O 1-227 [» ]
    2DYR X-ray 1.80 B/O 1-227 [» ]
    2DYS X-ray 2.20 B/O 1-227 [» ]
    2EIJ X-ray 1.90 B/O 1-227 [» ]
    2EIK X-ray 2.10 B/O 1-227 [» ]
    2EIL X-ray 2.10 B/O 1-227 [» ]
    2EIM X-ray 2.60 B/O 1-227 [» ]
    2EIN X-ray 2.70 B/O 1-227 [» ]
    2OCC X-ray 2.30 B/O 1-227 [» ]
    2Y69 X-ray 1.95 B/O 1-227 [» ]
    2YBB electron microscopy 19.00 M 1-227 [» ]
    2ZXW X-ray 2.50 B/O 1-227 [» ]
    3ABK X-ray 2.00 B/O 1-227 [» ]
    3ABL X-ray 2.10 B/O 1-227 [» ]
    3ABM X-ray 1.95 B/O 1-227 [» ]
    3AG1 X-ray 2.20 B/O 1-227 [» ]
    3AG2 X-ray 1.80 B/O 1-227 [» ]
    3AG3 X-ray 1.80 B/O 1-227 [» ]
    3AG4 X-ray 2.05 B/O 1-227 [» ]
    3ASN X-ray 3.00 B/O 1-227 [» ]
    3ASO X-ray 2.30 B/O 1-227 [» ]
    3WG7 X-ray 1.90 B/O 1-227 [» ]
    ProteinModelPortali P68530.
    SMRi P68530. Positions 1-227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-39024N.
    IntActi P68530. 1 interaction.

    Protein family/group databases

    TCDBi 3.D.4.7.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Proteomic databases

    PaxDbi P68530.
    PRIDEi P68530.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000060549 ; ENSBTAP00000053151 ; ENSBTAG00000043556 .
    GeneIDi 3283880.
    KEGGi bta:3283880.

    Organism-specific databases

    CTDi 4513.

    Phylogenomic databases

    eggNOGi COG1622.
    HOGENOMi HOG000264988.
    HOVERGENi HBG012727.
    InParanoidi P68530.
    KOi K02261.
    OMAi DHALMIV.
    OrthoDBi EOG7TJ3JX.
    TreeFami TF344269.

    Miscellaneous databases

    EvolutionaryTracei P68530.
    NextBioi 20810187.

    Family and domain databases

    Gene3Di 1.10.287.90. 1 hit.
    2.60.40.420. 1 hit.
    InterProi IPR001505. Copper_CuA.
    IPR008972. Cupredoxin.
    IPR014222. Cyt_c_oxidase_su2.
    IPR002429. Cyt_c_oxidase_su2_C.
    IPR011759. Cyt_c_oxidase_su2_TM_dom.
    [Graphical view ]
    Pfami PF00116. COX2. 1 hit.
    PF02790. COX2_TM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49503. SSF49503. 1 hit.
    SSF81464. SSF81464. 1 hit.
    TIGRFAMsi TIGR02866. CoxB. 1 hit.
    PROSITEi PS00078. COX2. 1 hit.
    PS50857. COX2_CUA. 1 hit.
    PS50999. COX2_TM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Studies on cytochrome c oxidase, IV[1-3]. Primary structure and function of subunit II."
      Steffens G.J., Buse G.
      Hoppe-Seyler's Z. Physiol. Chem. 360:613-619(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FORMYLATION AT MET-1.
      Tissue: Heart.
    2. "Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome."
      Anderson S., de Bruijn M.H.L., Coulson A.R., Eperon I.C., Sanger F., Young I.G.
      J. Mol. Biol. 156:683-717(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Heart.
    3. "The genetic code in bovine mitochondria: sequence of genes for the cytochrome oxidase subunit II and two tRNAs."
      Young I.G., Anderson S.
      Gene 12:257-265(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Bos taurus mitochondrial protein coding regions."
      Wettstein P.J.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 65, 66, D and F.
    5. "The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A."
      Tsukihara T., Aoyama H., Yamashita E., Tomizaki T., Yamaguchi H., Shinzawa-Itoh K., Nakashima R., Yaono R., Yoshikawa S.
      Science 272:1136-1144(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    6. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
      Tissue: Heart.
    7. "X-ray structure of azide-bound fully oxidized cytochrome c oxidase from bovine heart at 2.9 A resolution."
      Fei M.J., Yamashita E., Inoue N., Yao M., Yamaguchi H., Tsukihara T., Shinzawa-Itoh K., Nakashima R., Yoshikawa S.
      Acta Crystallogr. D 56:529-535(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
      Tissue: Heart.

    Entry informationi

    Entry nameiCOX2_BOVIN
    AccessioniPrimary (citable) accession number: P68530
    Secondary accession number(s): P00404
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3