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Protein

14-3-3 protein eta

Gene

Ywhah

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-111447. Activation of BAD and translocation to mitochondria.
R-RNO-1445148. Translocation of GLUT4 to the plasma membrane.
R-RNO-5625740. RHO GTPases activate PKNs.
R-RNO-5628897. TP53 Regulates Metabolic Genes.
R-RNO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein eta
Gene namesi
Name:Ywhah
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi3978. Ywhah.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 24624514-3-3 protein etaPRO_0000058625Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity
Modified residuei25 – 251PhosphoserineBy similarity
Modified residuei50 – 501N6-acetyllysineBy similarity
Modified residuei59 – 591PhosphoserineBy similarity
Modified residuei69 – 691N6-acetyllysineBy similarity
Modified residuei85 – 851Nitrated tyrosineBy similarity
Modified residuei120 – 1201N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.By similarity

Keywords - PTMi

Acetylation, Nitration, Phosphoprotein

Proteomic databases

PaxDbiP68511.
PRIDEiP68511.

PTM databases

iPTMnetiP68511.
PhosphoSiteiP68511.

Expressioni

Gene expression databases

GenevisibleiP68511. RN.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with ARHGEF28 and CDK16. Interacts with GAB2 (By similarity). Interacts with KCNK18 in a phosphorylation-dependent manner. Interacts with SAMSN1 (By similarity). Interacts with the 'Ser-241' phosphorylated form of PDPK1 (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei57 – 571Interaction with phosphoserine on interacting proteinBy similarity
Sitei132 – 1321Interaction with phosphoserine on interacting proteinBy similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NumbQ3MUI13EBI-444647,EBI-7007865

Protein-protein interaction databases

BioGridi247607. 4 interactions.
DIPiDIP-299N.
IntActiP68511. 6 interactions.
MINTiMINT-270381.
STRINGi10116.ENSRNOP00000024388.

Structurei

3D structure databases

ProteinModelPortaliP68511.
SMRiP68511. Positions 2-236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP68511.
KOiK16198.
OMAiAPEQACQ.
OrthoDBiEOG7HHWT3.
PhylomeDBiP68511.
TreeFamiTF102003.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68511-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK
60 70 80 90 100
NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV
110 120 130 140 150
LALLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS
160 170 180 190 200
EAAYKEAFEI SKEHMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA
210 220 230 240
FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN
Length:246
Mass (Da):28,212
Last modified:January 23, 2007 - v2
Checksum:iC12F62B4ABA76DA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17445 mRNA. Translation: BAA04259.1.
BC081825 mRNA. Translation: AAH81825.1.
PIRiA60031.
RefSeqiNP_037184.1. NM_013052.1.
UniGeneiRn.3324.

Genome annotation databases

EnsembliENSRNOT00000085735; ENSRNOP00000075701; ENSRNOG00000055471.
GeneIDi25576.
KEGGirno:25576.
UCSCiRGD:3978. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17445 mRNA. Translation: BAA04259.1.
BC081825 mRNA. Translation: AAH81825.1.
PIRiA60031.
RefSeqiNP_037184.1. NM_013052.1.
UniGeneiRn.3324.

3D structure databases

ProteinModelPortaliP68511.
SMRiP68511. Positions 2-236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247607. 4 interactions.
DIPiDIP-299N.
IntActiP68511. 6 interactions.
MINTiMINT-270381.
STRINGi10116.ENSRNOP00000024388.

PTM databases

iPTMnetiP68511.
PhosphoSiteiP68511.

Proteomic databases

PaxDbiP68511.
PRIDEiP68511.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000085735; ENSRNOP00000075701; ENSRNOG00000055471.
GeneIDi25576.
KEGGirno:25576.
UCSCiRGD:3978. rat.

Organism-specific databases

CTDi7533.
RGDi3978. Ywhah.

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP68511.
KOiK16198.
OMAiAPEQACQ.
OrthoDBiEOG7HHWT3.
PhylomeDBiP68511.
TreeFamiTF102003.

Enzyme and pathway databases

ReactomeiR-RNO-111447. Activation of BAD and translocation to mitochondria.
R-RNO-1445148. Translocation of GLUT4 to the plasma membrane.
R-RNO-5625740. RHO GTPases activate PKNs.
R-RNO-5628897. TP53 Regulates Metabolic Genes.
R-RNO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Miscellaneous databases

NextBioi607211.
PROiP68511.

Gene expression databases

GenevisibleiP68511. RN.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of cDNA to rat 14-3-3 eta chain polypeptide and the neuronal expression of the mRNA in the central nervous system."
    Watanabe M., Isobe T., Okuyama T., Ichimura T., Kuwano R., Takahashi Y., Kondo H.
    Brain Res. Mol. Brain Res. 10:151-158(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. Lubec G., Kang S.U., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 13-56; 62-69; 111-120; 126-132; 144-155; 163-172 AND 197-227, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry namei1433F_RAT
AccessioniPrimary (citable) accession number: P68511
Secondary accession number(s): P11576, P70198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.