P68511 (1433F_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3 protein eta

Gene names

Name:

Ywhah

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 By similarity.
Subunit structure	Homodimer By similarity. Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with ARHGEF28 and CDK16. Interacts with GAB2 By similarity. Interacts with KCNK18 in a phosphorylation-dependent manner. Interacts with SAMSN1 By similarity. Interacts with the 'Ser-241' phosphorylated form of PDPK1 By similarity.
Subcellular location	Cytoplasm.
Post-translational modification	Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion By similarity.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glucocorticoid catabolic process Inferred from electronic annotation. Source: Compara glucocorticoid receptor signaling pathway Inferred from electronic annotation. Source: Compara intracellular protein transport Inferred from electronic annotation. Source: Compara negative regulation of dendrite morphogenesis Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara regulation of sodium ion transmembrane transporter activity Inferred from sequence or structural similarity. Source: BHF-UCL
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ion channel binding Inferred from sequence or structural similarity. Source: BHF-UCL sodium channel regulator activity Inferred from sequence or structural similarity. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 246	245	14-3-3 protein eta		PRO_0000058625
Sites
	Site	57	1	Interaction with phosphoserine on interacting protein By similarity
	Site	132	1	Interaction with phosphoserine on interacting protein By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylglycine By similarity
	Modified residue	25	1	Phosphoserine By similarity
	Modified residue	59	1	Phosphoserine By similarity
	Modified residue	117	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68511 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C12F62B4ABA76DA3	FASTA	246	28,212
	10 20 30 40 50 60 MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW 70 80 90 100 110 120 RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LALLDKFLIK NCNDFQYESK 130 140 150 160 170 180 VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEHMQPTHP IRLGLALNFS 190 200 210 220 230 240 VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of cDNA to rat 14-3-3 eta chain polypeptide and the neuronal expression of the mRNA in the central nervous system." Watanabe M., Isobe T., Okuyama T., Ichimura T., Kuwano R., Takahashi Y., Kondo H. Brain Res. Mol. Brain Res. 10:151-158(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[3]	Lubec G., Kang S.U., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 13-56; 62-69; 111-120; 126-132; 144-155; 163-172 AND 197-227, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17445 mRNA. Translation: BAA04259.1. BC081825 mRNA. Translation: AAH81825.1.
IPI	IPI00231677.
PIR	A60031.
RefSeq	NP_037184.1. NM_013052.1.
UniGene	Rn.3324.
3D structure databases
ProteinModelPortal	P68511.
SMR	P68511. Positions 2-236.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-299N.
IntAct	P68511. 1 interaction.
MINT	MINT-270381.
STRING	10116.ENSRNOP00000024388.
PTM databases
PhosphoSite	P68511.
Proteomic databases
PaxDb	P68511.
PRIDE	P68511.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000024388; ENSRNOP00000024388; ENSRNOG00000026119.
GeneID	25576.
KEGG	rno:25576.
UCSC	RGD:3978. rat.
Organism-specific databases
CTD	7533.
RGD	3978. Ywhah.
Phylogenomic databases
eggNOG	COG5040.
GeneTree	ENSGT00690000101832.
HOGENOM	HOG000240379.
HOVERGEN	HBG050423.
InParanoid	P68511.
KO	K16198.
OMA	ESSEAAY.
OrthoDB	EOG48PMM0.
Gene expression databases
Genevestigator	P68511.
GermOnline	ENSRNOG00000026119. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.190.20. 1 hit.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607211.

Entry information

Entry name

1433F_RAT

Accession

Primary (citable) accession number: P68511
Secondary accession number(s): P11576, P70198

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program