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P68510 (1433F_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
14-3-3 protein eta
Gene names
Name:Ywhah
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 By similarity.

Subunit structure

Homodimer By similarity. Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B By similarity. Interacts with ARHGEF28 and CDK16. Interacts with KCNK18 in a phosphorylation-dependent manner. Interacts with SAMSN1. Interacts with the 'Ser-241' phosphorylated form of PDPK1 By similarity. Ref.1 Ref.9 Ref.10 Ref.11

Subcellular location

Cytoplasm.

Post-translational modification

Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Ref.8

Sequence similarities

Belongs to the 14-3-3 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcytoskeleton organization

Non-traceable author statement PubMed 12176032. Source: ProtInc

glucocorticoid catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

glucocorticoid receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

intracellular protein transport

Inferred from direct assay PubMed 12446771. Source: MGI

membrane depolarization during action potential

Inferred from sequence or structural similarity PubMed 16728661. Source: BHF-UCL

negative regulation of apoptotic process

Non-traceable author statement PubMed 12176032. Source: ProtInc

negative regulation of dendrite morphogenesis

Inferred from direct assay Ref.3. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of mitosis

Non-traceable author statement PubMed 12176032. Source: ProtInc

regulation of sodium ion transmembrane transporter activity

Inferred from sequence or structural similarity PubMed 16728661. Source: BHF-UCL

regulation of sodium ion transport

Inferred from sequence or structural similarity PubMed 16728661. Source: BHF-UCL

   Cellular_componentcytoplasm

Inferred from direct assay Ref.3. Source: MGI

cytoplasmic vesicle membrane

Traceable author statement. Source: Reactome

cytosol

Traceable author statement. Source: Reactome

extracellular vesicular exosome

Inferred from electronic annotation. Source: Ensembl

intercalated disc

Inferred by curator PubMed 16728661. Source: BHF-UCL

plasma membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionactin binding

Inferred from direct assay PubMed 12176032. Source: ProtInc

glucocorticoid receptor binding

Inferred from sequence or structural similarity. Source: UniProtKB

ion channel binding

Inferred from physical interaction PubMed 16728661. Source: BHF-UCL

protein binding

Inferred from physical interaction PubMed 16096643PubMed 21390248PubMed 9299557. Source: IntAct

protein domain specific binding

Inferred from direct assay PubMed 12446771. Source: MGI

sodium channel regulator activity

Inferred from sequence or structural similarity PubMed 16728661. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Lrrk2Q5S0064EBI-444641,EBI-2693710
Park2Q9WVS68EBI-444641,EBI-973635
SncaO550422EBI-444641,EBI-2310271
TNFAIP3P215803EBI-444641,EBI-527670From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 24624514-3-3 protein eta
PRO_0000058624

Sites

Site571Interaction with phosphoserine on interacting protein By similarity
Site1321Interaction with phosphoserine on interacting protein By similarity

Amino acid modifications

Modified residue21N-acetylglycine By similarity
Modified residue251Phosphoserine By similarity
Modified residue591Phosphoserine Ref.8

Experimental info

Sequence conflict15 – 162EQ → DE in BAA13422. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P68510 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: C12F62B4ABA76DA3

FASTA24628,212
        10         20         30         40         50         60 
MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW 

        70         80         90        100        110        120 
RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LALLDKFLIK NCNDFQYESK 

       130        140        150        160        170        180 
VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEHMQPTHP IRLGLALNFS 

       190        200        210        220        230        240 
VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE 


EAGEGN 

« Hide

References

« Hide 'large scale' references
[1]"The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins."
Sladeczek F., Camonis J.H., Burnol A.-F., Le Bouffant F.
Mol. Gen. Genet. 254:571-577(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CDK16.
Tissue: Brain.
[2]Takihara Y., Irie K., Nomura M., Motaleb M., Matsumoto K., Shimada K.
Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Sv.
[3]"Association of the TLX-2 homeodomain and 14-3-3eta signaling proteins."
Tang S.J., Seun T.-C., McInnes R.R., Buchwald M.
J. Biol. Chem. 273:25356-25363(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain."
Toyooka K., Muratake T., Watanabe H., Hayashi S., Ichikawa T., Usui H., Washiyama K., Kumanishi T., Takahashi Y.
Brain Res. Mol. Brain Res. 100:13-20(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Heart, Kidney and Sympathetic ganglion.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[7]Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 13-56; 62-69; 92-106; 111-120; 133-172 AND 199-227, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6 and OF1.
Tissue: Brain and Hippocampus.
[8]"A novel sphingosine-dependent protein kinase (SDK1) specifically phosphorylates certain isoforms of 14-3-3 protein."
Megidish T., Cooper J., Zhang L., Fu H., Hakomori S.
J. Biol. Chem. 273:21834-21845(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-59.
[9]"Identification of a novel interaction of 14-3-3 with p190RhoGEF."
Zhai J., Lin H., Shamim M., Schlaepfer W.W., Canete-Soler R.
J. Biol. Chem. 276:41318-41324(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARHGEF28.
[10]"Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK regulation."
Czirjak G., Vuity D., Enyedi P.
J. Biol. Chem. 283:15672-15680(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KCNK18.
[11]"SLy2 targets the nuclear SAP30/HDAC1 complex."
Brandt S., Ellwanger K., Beuter-Gunia C., Schuster M., Hausser A., Schmitz I., Beer-Hammer S.
Int. J. Biochem. Cell Biol. 42:1472-1481(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SAMSN1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U57311 mRNA. Translation: AAC53256.1.
D87661 mRNA. Translation: BAA13422.1.
AF077002 mRNA. Translation: AAC36290.1.
AB063572 Genomic DNA. Translation: BAB79599.1.
AK077596 mRNA. Translation: BAC36887.1.
AK149224 mRNA. Translation: BAE28768.1.
AK168520 mRNA. Translation: BAE40399.1.
AK169035 mRNA. Translation: BAE40826.1.
AK169189 mRNA. Translation: BAE40965.1.
BC008187 mRNA. Translation: AAH08187.1.
BC061497 mRNA. Translation: AAH61497.1.
CCDSCCDS19198.1.
RefSeqNP_035868.1. NM_011738.2.
UniGeneMm.332314.

3D structure databases

ProteinModelPortalP68510.
SMRP68510. Positions 2-236.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204621. 7 interactions.
IntActP68510. 315 interactions.
MINTMINT-198030.

PTM databases

PhosphoSiteP68510.

2D gel databases

UCD-2DPAGEP68510.

Proteomic databases

MaxQBP68510.
PaxDbP68510.
PRIDEP68510.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019109; ENSMUSP00000019109; ENSMUSG00000018965.
GeneID22629.
KEGGmmu:22629.
UCSCuc008xag.2. mouse.

Organism-specific databases

CTD7533.
MGIMGI:109194. Ywhah.

Phylogenomic databases

eggNOGCOG5040.
GeneTreeENSGT00730000110613.
HOGENOMHOG000240379.
HOVERGENHBG050423.
InParanoidP68510.
KOK16198.
OMAESSEAAY.
OrthoDBEOG7HHWT3.
PhylomeDBP68510.
TreeFamTF102003.

Enzyme and pathway databases

ReactomeREACT_147847. Translocation of Glut4 to the Plasma Membrane.

Gene expression databases

BgeeP68510.
GenevestigatorP68510.

Family and domain databases

Gene3D1.20.190.20. 1 hit.
InterProIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERPTHR18860. PTHR18860. 1 hit.
PfamPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFPIRSF000868. 14-3-3. 1 hit.
PRINTSPR00305. 1433ZETA.
SMARTSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMSSF48445. SSF48445. 1 hit.
PROSITEPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302995.
PROP68510.
SOURCESearch...

Entry information

Entry name1433F_MOUSE
AccessionPrimary (citable) accession number: P68510
Secondary accession number(s): P11576, P70198, Q3TGZ9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot