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Protein

14-3-3 protein eta

Gene

Ywhah

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity).By similarity

GO - Molecular functioni

  • actin binding Source: ProtInc
  • enzyme binding Source: MGI
  • glucocorticoid receptor binding Source: UniProtKB
  • identical protein binding Source: MGI
  • ion channel binding Source: BHF-UCL
  • protein domain specific binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • sodium channel regulator activity Source: BHF-UCL

GO - Biological processi

  • cytoskeleton organization Source: ProtInc
  • glucocorticoid catabolic process Source: UniProtKB
  • glucocorticoid receptor signaling pathway Source: UniProtKB
  • intracellular protein transport Source: MGI
  • membrane depolarization during action potential Source: BHF-UCL
  • negative regulation of apoptotic process Source: ProtInc
  • negative regulation of dendrite morphogenesis Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of mitotic nuclear division Source: ProtInc
  • regulation of sodium ion transmembrane transporter activity Source: BHF-UCL
  • regulation of sodium ion transport Source: BHF-UCL

Enzyme and pathway databases

ReactomeiR-MMU-111447 Activation of BAD and translocation to mitochondria
R-MMU-1445148 Translocation of GLUT4 to the plasma membrane
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein eta
Gene namesi
Name:Ywhah
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:109194 Ywhah

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000586242 – 24614-3-3 protein etaAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1
Modified residuei25PhosphoserineBy similarity1
Modified residuei50N6-acetyllysine; alternateBy similarity1
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei59Phosphoserine1 Publication1
Modified residuei69N6-acetyllysineBy similarity1
Modified residuei85Nitrated tyrosineBy similarity1
Modified residuei120N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Nitration, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP68510
MaxQBiP68510
PaxDbiP68510
PeptideAtlasiP68510
PRIDEiP68510
TopDownProteomicsiP68510

2D gel databases

UCD-2DPAGEiP68510

PTM databases

iPTMnetiP68510
PhosphoSitePlusiP68510
SwissPalmiP68510

Expressioni

Gene expression databases

BgeeiENSMUSG00000018965
GenevisibleiP68510 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B (By similarity). Interacts with ARHGEF28 and CDK16. Interacts with KCNK18 in a phosphorylation-dependent manner. Interacts with SAMSN1. Interacts with the 'Ser-241' phosphorylated form of PDPK1 (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with SLITRK1 (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Interaction with phosphoserine on interacting proteinBy similarity1
Sitei132Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin binding Source: ProtInc
  • enzyme binding Source: MGI
  • glucocorticoid receptor binding Source: UniProtKB
  • identical protein binding Source: MGI
  • ion channel binding Source: BHF-UCL
  • protein domain specific binding Source: MGI
  • protein heterodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi204621, 13 interactors
IntActiP68510, 321 interactors
MINTiP68510
STRINGi10090.ENSMUSP00000019109

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 16Combined sources14
Helixi20 – 32Combined sources13
Helixi39 – 62Combined sources24
Turni66 – 68Combined sources3
Turni76 – 78Combined sources3
Helixi82 – 106Combined sources25
Turni107 – 111Combined sources5
Helixi117 – 137Combined sources21
Helixi140 – 164Combined sources25
Helixi170 – 185Combined sources16
Helixi190 – 207Combined sources18
Helixi218 – 232Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5YQGX-ray2.10A/B/C/D1-246[»]
ProteinModelPortaliP68510
SMRiP68510
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841 Eukaryota
COG5040 LUCA
GeneTreeiENSGT00760000119116
HOGENOMiHOG000240379
HOVERGENiHBG050423
InParanoidiP68510
KOiK16198
OMAiCERYEDM
OrthoDBiEOG091G0VKY
PhylomeDBiP68510
TreeFamiTF102003

Family and domain databases

Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68510-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK
60 70 80 90 100
NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV
110 120 130 140 150
LALLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS
160 170 180 190 200
EAAYKEAFEI SKEHMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA
210 220 230 240
FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN
Length:246
Mass (Da):28,212
Last modified:January 23, 2007 - v2
Checksum:iC12F62B4ABA76DA3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15 – 16EQ → DE in BAA13422 (Ref. 2) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57311 mRNA Translation: AAC53256.1
D87661 mRNA Translation: BAA13422.1
AF077002 mRNA Translation: AAC36290.1
AB063572 Genomic DNA Translation: BAB79599.1
AK077596 mRNA Translation: BAC36887.1
AK149224 mRNA Translation: BAE28768.1
AK168520 mRNA Translation: BAE40399.1
AK169035 mRNA Translation: BAE40826.1
AK169189 mRNA Translation: BAE40965.1
BC008187 mRNA Translation: AAH08187.1
BC061497 mRNA Translation: AAH61497.1
CCDSiCCDS19198.1
RefSeqiNP_035868.1, NM_011738.2
UniGeneiMm.332314

Genome annotation databases

EnsembliENSMUST00000019109; ENSMUSP00000019109; ENSMUSG00000018965
GeneIDi22629
KEGGimmu:22629
UCSCiuc008xag.2 mouse

Similar proteinsi

Entry informationi

Entry namei1433F_MOUSE
AccessioniPrimary (citable) accession number: P68510
Secondary accession number(s): P11576, P70198, Q3TGZ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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