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Protein

14-3-3 protein eta

Gene

Ywhah

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei57 – 571Interaction with phosphoserine on interacting proteinBy similarity
Sitei132 – 1321Interaction with phosphoserine on interacting proteinBy similarity

GO - Molecular functioni

  1. actin binding Source: ProtInc
  2. enzyme binding Source: MGI
  3. glucocorticoid receptor binding Source: UniProtKB
  4. ion channel binding Source: BHF-UCL
  5. protein domain specific binding Source: MGI
  6. protein heterodimerization activity Source: MGI
  7. sodium channel regulator activity Source: BHF-UCL

GO - Biological processi

  1. cytoskeleton organization Source: ProtInc
  2. glucocorticoid catabolic process Source: UniProtKB
  3. glucocorticoid receptor signaling pathway Source: UniProtKB
  4. intracellular protein transport Source: MGI
  5. membrane depolarization during action potential Source: BHF-UCL
  6. negative regulation of apoptotic process Source: ProtInc
  7. negative regulation of dendrite morphogenesis Source: MGI
  8. positive regulation of transcription, DNA-templated Source: UniProtKB
  9. regulation of mitosis Source: ProtInc
  10. regulation of sodium ion transmembrane transporter activity Source: BHF-UCL
  11. regulation of sodium ion transport Source: BHF-UCL
  12. substantia nigra development Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein eta
Gene namesi
Name:Ywhah
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:109194. Ywhah.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytoplasmic vesicle membrane Source: Reactome
  3. cytosol Source: Reactome
  4. extracellular vesicular exosome Source: MGI
  5. intercalated disc Source: BHF-UCL
  6. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 24624514-3-3 protein etaPRO_0000058624Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity
Modified residuei25 – 251PhosphoserineBy similarity
Modified residuei50 – 501N6-acetyllysineBy similarity
Modified residuei59 – 591Phosphoserine1 Publication
Modified residuei69 – 691N6-acetyllysineBy similarity
Modified residuei85 – 851Nitrated tyrosineBy similarity
Modified residuei120 – 1201N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.1 Publication

Keywords - PTMi

Acetylation, Nitration, Phosphoprotein

Proteomic databases

MaxQBiP68510.
PaxDbiP68510.
PRIDEiP68510.

2D gel databases

UCD-2DPAGEP68510.

PTM databases

PhosphoSiteiP68510.

Expressioni

Gene expression databases

BgeeiP68510.
GenevestigatoriP68510.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B (By similarity). Interacts with ARHGEF28 and CDK16. Interacts with KCNK18 in a phosphorylation-dependent manner. Interacts with SAMSN1. Interacts with the 'Ser-241' phosphorylated form of PDPK1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Lrrk2Q5S0064EBI-444641,EBI-2693710
Park2Q9WVS68EBI-444641,EBI-973635
SncaO550422EBI-444641,EBI-2310271
TNFAIP3P215803EBI-444641,EBI-527670From a different organism.

Protein-protein interaction databases

BioGridi204621. 9 interactions.
IntActiP68510. 315 interactions.
MINTiMINT-198030.

Structurei

3D structure databases

ProteinModelPortaliP68510.
SMRiP68510. Positions 2-236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiCOG5040.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP68510.
KOiK16198.
OMAiAPEQACQ.
OrthoDBiEOG7HHWT3.
PhylomeDBiP68510.
TreeFamiTF102003.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68510-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK
60 70 80 90 100
NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV
110 120 130 140 150
LALLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS
160 170 180 190 200
EAAYKEAFEI SKEHMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA
210 220 230 240
FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN
Length:246
Mass (Da):28,212
Last modified:January 23, 2007 - v2
Checksum:iC12F62B4ABA76DA3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 162EQ → DE in BAA13422 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57311 mRNA. Translation: AAC53256.1.
D87661 mRNA. Translation: BAA13422.1.
AF077002 mRNA. Translation: AAC36290.1.
AB063572 Genomic DNA. Translation: BAB79599.1.
AK077596 mRNA. Translation: BAC36887.1.
AK149224 mRNA. Translation: BAE28768.1.
AK168520 mRNA. Translation: BAE40399.1.
AK169035 mRNA. Translation: BAE40826.1.
AK169189 mRNA. Translation: BAE40965.1.
BC008187 mRNA. Translation: AAH08187.1.
BC061497 mRNA. Translation: AAH61497.1.
CCDSiCCDS19198.1.
RefSeqiNP_035868.1. NM_011738.2.
UniGeneiMm.332314.

Genome annotation databases

EnsembliENSMUST00000019109; ENSMUSP00000019109; ENSMUSG00000018965.
GeneIDi22629.
KEGGimmu:22629.
UCSCiuc008xag.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57311 mRNA. Translation: AAC53256.1.
D87661 mRNA. Translation: BAA13422.1.
AF077002 mRNA. Translation: AAC36290.1.
AB063572 Genomic DNA. Translation: BAB79599.1.
AK077596 mRNA. Translation: BAC36887.1.
AK149224 mRNA. Translation: BAE28768.1.
AK168520 mRNA. Translation: BAE40399.1.
AK169035 mRNA. Translation: BAE40826.1.
AK169189 mRNA. Translation: BAE40965.1.
BC008187 mRNA. Translation: AAH08187.1.
BC061497 mRNA. Translation: AAH61497.1.
CCDSiCCDS19198.1.
RefSeqiNP_035868.1. NM_011738.2.
UniGeneiMm.332314.

3D structure databases

ProteinModelPortaliP68510.
SMRiP68510. Positions 2-236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204621. 9 interactions.
IntActiP68510. 315 interactions.
MINTiMINT-198030.

PTM databases

PhosphoSiteiP68510.

2D gel databases

UCD-2DPAGEP68510.

Proteomic databases

MaxQBiP68510.
PaxDbiP68510.
PRIDEiP68510.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019109; ENSMUSP00000019109; ENSMUSG00000018965.
GeneIDi22629.
KEGGimmu:22629.
UCSCiuc008xag.2. mouse.

Organism-specific databases

CTDi7533.
MGIiMGI:109194. Ywhah.

Phylogenomic databases

eggNOGiCOG5040.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP68510.
KOiK16198.
OMAiAPEQACQ.
OrthoDBiEOG7HHWT3.
PhylomeDBiP68510.
TreeFamiTF102003.

Enzyme and pathway databases

ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

ChiTaRSiYwhah. mouse.
NextBioi302995.
PROiP68510.
SOURCEiSearch...

Gene expression databases

BgeeiP68510.
GenevestigatoriP68510.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins."
    Sladeczek F., Camonis J.H., Burnol A.-F., Le Bouffant F.
    Mol. Gen. Genet. 254:571-577(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CDK16.
    Tissue: Brain.
  2. Takihara Y., Irie K., Nomura M., Motaleb M., Matsumoto K., Shimada K.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Sv.
  3. "Association of the TLX-2 homeodomain and 14-3-3eta signaling proteins."
    Tang S.J., Seun T.-C., McInnes R.R., Buchwald M.
    J. Biol. Chem. 273:25356-25363(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain."
    Toyooka K., Muratake T., Watanabe H., Hayashi S., Ichikawa T., Usui H., Washiyama K., Kumanishi T., Takahashi Y.
    Brain Res. Mol. Brain Res. 100:13-20(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart, Kidney and Sympathetic ganglion.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  7. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 13-56; 62-69; 92-106; 111-120; 133-172 AND 199-227, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.
  8. "A novel sphingosine-dependent protein kinase (SDK1) specifically phosphorylates certain isoforms of 14-3-3 protein."
    Megidish T., Cooper J., Zhang L., Fu H., Hakomori S.
    J. Biol. Chem. 273:21834-21845(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-59.
  9. "Identification of a novel interaction of 14-3-3 with p190RhoGEF."
    Zhai J., Lin H., Shamim M., Schlaepfer W.W., Canete-Soler R.
    J. Biol. Chem. 276:41318-41324(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARHGEF28.
  10. "Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK regulation."
    Czirjak G., Vuity D., Enyedi P.
    J. Biol. Chem. 283:15672-15680(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCNK18.
  11. Cited for: INTERACTION WITH SAMSN1.

Entry informationi

Entry namei1433F_MOUSE
AccessioniPrimary (citable) accession number: P68510
Secondary accession number(s): P11576, P70198, Q3TGZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.