P68509 (1433F_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3 protein eta

Alternative name(s):
Protein kinase C inhibitor protein 1
Short name=KCIP-1

Gene names

Name:

YWHAH

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 By similarity. Ref.4
Subunit structure	Homodimer By similarity. Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with ARHGEF28 and CDK16. Interacts with GAB2 By similarity. Interacts with KCNK18 in a phosphorylation-dependent manner. Interacts with SAMSN1 By similarity. Interacts with the 'Ser-241' phosphorylated form of PDPK1 By similarity.
Subcellular location	Cytoplasm.
Post-translational modification	Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion By similarity.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 246	245	14-3-3 protein eta		PRO_0000058622
Sites
	Site	57	1	Interaction with phosphoserine on interacting protein By similarity
	Site	132	1	Interaction with phosphoserine on interacting protein By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylglycine By similarity
	Modified residue	25	1	Phosphoserine By similarity
	Modified residue	59	1	Phosphoserine By similarity
	Modified residue	117	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	141	1	E → Q in AAI02983. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P68509 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C12F62B4ABA76DA3	FASTA	246	28,212
	10 20 30 40 50 60 MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW 70 80 90 100 110 120 RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LALLDKFLIK NCNDFQYESK 130 140 150 160 170 180 VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEHMQPTHP IRLGLALNFS 190 200 210 220 230 240 VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of cDNA coding for brain-specific 14-3-3 protein, a protein kinase-dependent activator of tyrosine and tryptophan hydroxylases." Ichimura T., Isobe T., Okuyama T., Takahashi N., Araki K., Kuwano R., Takahashi Y. Proc. Natl. Acad. Sci. U.S.A. 85:7084-7088(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Cerebellum.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.
[3]	"Kinase and neurotransmitters." Aitken A., Ellis C.A., Harris A., Sellers L.A., Toker A. Nature 344:594-594(1990) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY TO KINASE INHIBITOR.
[4]	"Activation of protein kinase C by purified bovine brain 14-3-3: comparison with tyrosine hydroxylase activation." Tanji M., Horwitz R., Rosenfeld G., Waymire J.C. J. Neurochem. 63:1908-1916(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03868 mRNA. Translation: AAA30347.1. BC102982 mRNA. Translation: AAI02983.1.
IPI	IPI00695448.
PIR	A40484.
RefSeq	NP_776917.2. NM_174492.2.
UniGene	Bt.7225.
3D structure databases
ProteinModelPortal	P68509.
SMR	P68509. Positions 2-236.
ModBase	Search...
Proteomic databases
PaxDb	P68509.
PRIDE	P68509.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	282126.
KEGG	bta:282126.
Organism-specific databases
CTD	7533.
Phylogenomic databases
eggNOG	COG5040.
HOGENOM	HOG000240379.
HOVERGEN	HBG050423.
InParanoid	P68509.
KO	K16198.
OrthoDB	EOG48PMM0.
Family and domain databases
Gene3D	1.20.190.20. 1 hit.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20805963.

Entry information

Entry name

1433F_BOVIN

Accession

Primary (citable) accession number: P68509
Secondary accession number(s): P11576, P70198, Q3ZC14

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program