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Protein

Metallothionein B

Gene

mtb

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi4 – 41Divalent metal cation; cluster B
Metal bindingi6 – 61Divalent metal cation; cluster B
Metal bindingi12 – 121Divalent metal cation; cluster B
Metal bindingi14 – 141Divalent metal cation; cluster B
Metal bindingi18 – 181Divalent metal cation; cluster B
Metal bindingi20 – 201Divalent metal cation; cluster B
Metal bindingi23 – 231Divalent metal cation; cluster B
Metal bindingi25 – 251Divalent metal cation; cluster B
Metal bindingi28 – 281Divalent metal cation; cluster B
Metal bindingi32 – 321Divalent metal cation; cluster A
Metal bindingi33 – 331Divalent metal cation; cluster A
Metal bindingi35 – 351Divalent metal cation; cluster A
Metal bindingi36 – 361Divalent metal cation; cluster A
Metal bindingi40 – 401Divalent metal cation; cluster A
Metal bindingi43 – 431Divalent metal cation; cluster A
Metal bindingi47 – 471Divalent metal cation; cluster A
Metal bindingi49 – 491Divalent metal cation; cluster A
Metal bindingi54 – 541Divalent metal cation; cluster A
Metal bindingi58 – 581Divalent metal cation; cluster A
Metal bindingi59 – 591Divalent metal cation; cluster A

GO - Molecular functioni

GO - Biological processi

  • response to cadmium ion Source: AgBase
  • response to copper ion Source: AgBase
  • response to mercury ion Source: AgBase
  • response to zinc ion Source: AgBase
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Metal-thiolate cluster

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein B
Short name:
MT-B
Gene namesi
Name:mtb
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Subcellular locationi

GO - Cellular componenti

  • cell Source: AgBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6060Metallothionein BPRO_0000197296Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP68501.
SMRiP68501. Positions 2-29, 31-60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2828BetaAdd
BLAST
Regioni29 – 6032AlphaAdd
BLAST

Domaini

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG096123.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR003019. Metalthion.
IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68501-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPCECSKTG SCNCGGSCKC SNCACTSCKK SCCPCCPSDC SKCASGCVCK
60
GKTCDTSCCQ
Length:60
Mass (Da):6,033
Last modified:November 23, 2004 - v1
Checksum:i9EA1E70FBE59B4EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18104 mRNA. Translation: AAA49566.1.
M22487 Genomic DNA. Translation: AAA49567.1.
X59394 mRNA. Translation: CAA42037.1.
PIRiA30818. B27490.
UniGeneiOmy.11783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18104 mRNA. Translation: AAA49566.1.
M22487 Genomic DNA. Translation: AAA49567.1.
X59394 mRNA. Translation: CAA42037.1.
PIRiA30818. B27490.
UniGeneiOmy.11783.

3D structure databases

ProteinModelPortaliP68501.
SMRiP68501. Positions 2-29, 31-60.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG096123.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR003019. Metalthion.
IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The rainbow trout metallothioneins: molecular cloning and characterization of two distinct cDNA sequences."
    Bonham K., Zafarullah M., Gedamu L.
    DNA 6:519-528(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure of the rainbow trout metallothionein B gene and characterization of its metal-responsive region."
    Zafarullah M., Bonham K., Gedamu L.
    Mol. Cell. Biol. 8:4469-4476(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Elucidation of cDNA sequences for metallothioneins from rainbow trout, stone loach and pike liver using the polymerase chain reaction."
    Kille P., Stephens P.E., Kay J.
    Biochim. Biophys. Acta 1089:407-410(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiMTB_ONCMY
AccessioniPrimary (citable) accession number: P68501
Secondary accession number(s): P09862
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: December 9, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.