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P68460 (GLRX2_VACCW) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaredoxin-2
Gene names
Ordered Locus Names:VACWR081
ORF Names:G4L
OrganismVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) [Reference proteome]
Taxonomic identifier10254 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length124 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glutaredoxin necessary for virion morphogenesis and virus replication. Functions as a thiol-disulfide transfer protein between membrane-associated A2.5 and substrates L1 or F9. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of E10, A2.5 and G4. Exhibit thioltransferase and dehydroascorbate reductase activities in vitro. Ref.3 Ref.4 Ref.5 Ref.6

Subunit structure

Homodimer Probable. Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer. Interacts with L1; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer. Ref.6

Subcellular location

Host cytoplasm.

Sequence similarities

Belongs to the glutaredoxin family.

Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentHost cytoplasm
   DomainRedox-active center
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processoxidation-reduction process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 124124Glutaredoxin-2
PRO_0000141629

Amino acid modifications

Disulfide bond13 ↔ 16Redox-active Ref.5

Experimental info

Mutagenesis131C → S: Complete loss of substrate oxidation. Ref.5
Mutagenesis161C → S: Partial loss of substrate oxidation. Ref.5

Secondary structure

...................... 124
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P68460 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: DFBE2D7B3A1A9CA6

FASTA12413,987
        10         20         30         40         50         60 
MKNVLIIFGK PYCSICENVS DAVEELKSEY DILHVDILSF FLKDGDSSML GDVKRGTLIG 

        70         80         90        100        110        120 
NFAAHLSNYI VSIFKYNPQT KQMAFVDINK SLDFTKTDKS LVNLEILKSE IEKATYGVWP 


PVTE 

« Hide

References

« Hide 'large scale' references
[1]"Genetic and molecular biological characterization of a vaccinia virus gene which renders the virus dependent on isatin-beta-thiosemicarbazone (IBT)."
Meis R.J., Condit R.C.
Virology 182:442-454(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Vaccinia virus G4L gene encodes a second glutaredoxin."
Gvakharia B.O., Koonin E.K., Mathews C.K.
Virology 226:408-411(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"A glutaredoxin, encoded by the G4L gene of vaccinia virus, is essential for virion morphogenesis."
White C.L., Weisberg A.S., Moss B.
J. Virol. 74:9175-9183(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Vaccinia virus G4L glutaredoxin is an essential intermediate of a cytoplasmic disulfide bond pathway required for virion assembly."
White C.L., Senkevich T.G., Moss B.
J. Virol. 76:467-472(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISULFIDE BOND, MUTAGENESIS OF CYS-13 AND CYS-16.
[6]"Complete pathway for protein disulfide bond formation encoded by poxviruses."
Senkevich T.G., White C.L., Koonin E.V., Moss B.
Proc. Natl. Acad. Sci. U.S.A. 99:6667-6672(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH A2.5, INTERACTION WITH L1.
[7]"The structure of G4, the poxvirus disulfide oxidoreductase essential for virus maturation and infectivity."
Su H.P., Lin D.Y., Garboczi D.N.
J. Virol. 80:7706-7713(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03399 Genomic DNA. Translation: AAB59814.1.
AY243312 Genomic DNA. Translation: AAO89360.1.
RefSeqYP_232963.1. NC_006998.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2G2QX-ray2.50A/B/C1-124[»]
ProteinModelPortalP68460.
SMRP68460. Positions 1-121.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3707537.

Phylogenomic databases

ProtClustDBPHA3075.

Family and domain databases

Gene3D3.40.30.10. 1 hit.
InterProIPR008554. Glutaredoxin-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF05768. DUF836. 1 hit.
[Graphical view]
SUPFAMSSF52833. SSF52833. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP68460.

Entry information

Entry nameGLRX2_VACCW
AccessionPrimary (citable) accession number: P68460
Secondary accession number(s): P21025, Q76ZU1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: October 16, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references