P68460 (GLRX2_VACCW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaredoxin-2

Gene names

Ordered Locus Names:	VACWR081
ORF Names:	G4L

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) [Reference proteome]

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glutaredoxin necessary for virion morphogenesis and virus replication. Functions as a thiol-disulfide transfer protein between membrane-associated A2.5 and substrates L1 or F9. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of E10, A2.5 and G4. Exhibit thioltransferase and dehydroascorbate reductase activities in vitro. Ref.3 Ref.4 Ref.5 Ref.6
Subunit structure	Homodimer Probable. Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer. Interacts with L1; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer. Ref.6
Subcellular location	Host cytoplasm.
Sequence similarities	Belongs to the glutaredoxin family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Host cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 124

124

Glutaredoxin-2

PRO_0000141629

Amino acid modifications

Disulfide bond

13 ↔ 16

Redox-active Ref.5

Experimental info

Mutagenesis

13

1

C → S: Complete loss of substrate oxidation. Ref.5

Mutagenesis

16

1

C → S: Partial loss of substrate oxidation. Ref.5

Secondary structure

1

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124

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Helix

14 – 24

11

Turn

25 – 29

5

Beta strand

30 – 36

7

Helix

46 – 48

3

Helix

58 – 65

8

Helix

66 – 69

4

Beta strand

71 – 77

7

Turn

78 – 81

4

Beta strand

82 – 85

4

Helix

89 – 91

3

Helix

99 – 101

3

Helix

104 – 113

10

Sequences

	Sequence		Length	Mass (Da)
	P68460 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: DFBE2D7B3A1A9CA6	FASTA	124	13,987
	10 20 30 40 50 60 MKNVLIIFGK PYCSICENVS DAVEELKSEY DILHVDILSF FLKDGDSSML GDVKRGTLIG 70 80 90 100 110 120 NFAAHLSNYI VSIFKYNPQT KQMAFVDINK SLDFTKTDKS LVNLEILKSE IEKATYGVWP PVTE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic and molecular biological characterization of a vaccinia virus gene which renders the virus dependent on isatin-beta-thiosemicarbazone (IBT)." Meis R.J., Condit R.C. Virology 182:442-454(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Vaccinia virus G4L gene encodes a second glutaredoxin." Gvakharia B.O., Koonin E.K., Mathews C.K. Virology 226:408-411(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"A glutaredoxin, encoded by the G4L gene of vaccinia virus, is essential for virion morphogenesis." White C.L., Weisberg A.S., Moss B. J. Virol. 74:9175-9183(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Vaccinia virus G4L glutaredoxin is an essential intermediate of a cytoplasmic disulfide bond pathway required for virion assembly." White C.L., Senkevich T.G., Moss B. J. Virol. 76:467-472(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISULFIDE BOND, MUTAGENESIS OF CYS-13 AND CYS-16.
[6]	"Complete pathway for protein disulfide bond formation encoded by poxviruses." Senkevich T.G., White C.L., Koonin E.V., Moss B. Proc. Natl. Acad. Sci. U.S.A. 99:6667-6672(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH A2.5, INTERACTION WITH L1.
[7]	"The structure of G4, the poxvirus disulfide oxidoreductase essential for virus maturation and infectivity." Su H.P., Lin D.Y., Garboczi D.N. J. Virol. 80:7706-7713(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J03399 Genomic DNA. Translation: AAB59814.1.
AY243312 Genomic DNA. Translation: AAO89360.1.

RefSeq

YP_232963.1. NC_006998.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2G2Q	X-ray	2.50	A/B/C	1-124	[»]

ProteinModelPortal

P68460.

SMR

P68460. Positions 1-121.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3707537.

Phylogenomic databases

ProtClustDB

PHA3075.

Family and domain databases

Gene3D

3.40.30.10. 1 hit.

InterPro

IPR008554. Glutaredoxin-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Pfam

PF05768. DUF836. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P68460.

Entry information

Entry name

GLRX2_VACCW

Accession

Primary (citable) accession number: P68460
Secondary accession number(s): P21025, Q76ZU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program