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Protein

Omega-theraphotoxin-Hs1a

Gene
N/A
Organism
Haplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly blocks N-type calcium channels (Cav2.2/CACNA1B) in rat dorsal root ganglion cells (IC50=40 nM). Elicits no toxic symptoms in either vertebrates or invertebrates during a period of 48 hours post-injection, when it was assayed in vivo by direct injection into mice and cockroaches.1 Publication

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

Protein family/group databases

TCDBi8.B.4.1.5. the conotoxin t (conotoxin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-theraphotoxin-Hs1a
Short name:
Omega-TRTX-Hs1a
Alternative name(s):
Huwentoxin-10
Huwentoxin-X
Short name:
HwTx-X
OrganismiHaplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
Taxonomic identifieri29017 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeHaplopelma

Organism-specific databases

ArachnoServeriAS000325. omega-theraphotoxin-Hs1a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 40201 PublicationPRO_0000249420Add
BLAST
Peptidei41 – 6828Omega-theraphotoxin-Hs1aPRO_0000045010Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 591 Publication
Disulfide bondi49 ↔ 621 Publication
Disulfide bondi58 ↔ 671 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
68
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi58 – 603Combined sources
Beta strandi62 – 654Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y29NMR-A41-68[»]
ProteinModelPortaliP68424.
SMRiP68424. Positions 41-68.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68424.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Keywords - Domaini

Knottin, Signal

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMKILVLVA VLCLVVSTHA ERHSKTDMED MEDSPMIQER KCLPPGKPCY
60
GATQKIPCCG VCSHNKCT
Length:68
Mass (Da):7,521
Last modified:September 5, 2006 - v2
Checksum:i5E1BB224C5B6B803
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 293Missing in ABY77740 (PubMed:18482741).Curated

Mass spectrometryi

Molecular mass is 2931.34 Da from positions 21 - 68. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU195287 mRNA. Translation: ABY77740.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU195287 mRNA. Translation: ABY77740.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y29NMR-A41-68[»]
ProteinModelPortaliP68424.
SMRiP68424. Positions 41-68.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.4.1.5. the conotoxin t (conotoxin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000325. omega-theraphotoxin-Hs1a.

Miscellaneous databases

EvolutionaryTraceiP68424.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Function and solution structure of Huwentoxin-X, a specific blocker of N-type calcium channels, from the Chinese bird spider Ornithoctonus huwena."
    Liu Z., Dai J., Dai L., Deng M., Hu Z., Hu W., Liang S.
    J. Biol. Chem. 281:8628-8635(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 41-68, SYNTHESIS OF 41-68, FUNCTION, MASS SPECTROMETRY, STRUCTURE BY NMR OF 41-68, DISULFIDE BONDS.
    Tissue: Venom and Venom gland.
  2. "Molecular diversification based on analysis of expressed sequence tags from the venom glands of the Chinese bird spider Ornithoctonus huwena."
    Jiang L., Peng L., Chen J., Zhang Y., Xiong X., Liang S.
    Toxicon 51:1479-1489(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTXH10_HAPSC
AccessioniPrimary (citable) accession number: P68424
Secondary accession number(s): B3FIU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: September 5, 2006
Last modified: December 9, 2015
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.