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Protein

Omega-theraphotoxin-Hs1a

Gene
N/A
Organism
Haplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly blocks N-type calcium channels (Cav2.2/CACNA1B) in rat dorsal root ganglion cells (IC50=40 nM). Elicits no toxic symptoms in either vertebrates or invertebrates during a period of 48 hours post-injection, when it was assayed in vivo by direct injection into mice and cockroaches.1 Publication

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

Protein family/group databases

TCDBi8.B.4.1.5. the conotoxin t (conotoxin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-theraphotoxin-Hs1a
Short name:
Omega-TRTX-Hs1a
Alternative name(s):
Huwentoxin-10
Huwentoxin-X
Short name:
HwTx-X
OrganismiHaplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
Taxonomic identifieri29017 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeHaplopelma

Organism-specific databases

ArachnoServeriAS000325. omega-theraphotoxin-Hs1a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000024942021 – 401 PublicationAdd BLAST20
PeptideiPRO_000004501041 – 68Omega-theraphotoxin-Hs1aAdd BLAST28

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 591 Publication
Disulfide bondi49 ↔ 621 Publication
Disulfide bondi58 ↔ 671 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi58 – 60Combined sources3
Beta strandi62 – 65Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y29NMR-A41-68[»]
ProteinModelPortaliP68424.
SMRiP68424.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68424.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Keywords - Domaini

Knottin, Signal

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMKILVLVA VLCLVVSTHA ERHSKTDMED MEDSPMIQER KCLPPGKPCY
60
GATQKIPCCG VCSHNKCT
Length:68
Mass (Da):7,521
Last modified:September 5, 2006 - v2
Checksum:i5E1BB224C5B6B803
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27 – 29Missing in ABY77740 (PubMed:18482741).Curated3

Mass spectrometryi

Molecular mass is 2931.34 Da from positions 21 - 68. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU195287 mRNA. Translation: ABY77740.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU195287 mRNA. Translation: ABY77740.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y29NMR-A41-68[»]
ProteinModelPortaliP68424.
SMRiP68424.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.4.1.5. the conotoxin t (conotoxin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000325. omega-theraphotoxin-Hs1a.

Miscellaneous databases

EvolutionaryTraceiP68424.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiTXH10_HAPSC
AccessioniPrimary (citable) accession number: P68424
Secondary accession number(s): B3FIU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: September 5, 2006
Last modified: November 2, 2016
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.