P68402 (PA1B2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Platelet-activating factor acetylhydrolase IB subunit beta
EC=3.1.1.47

Alternative name(s):
PAF acetylhydrolase 30 kDa subunit
Short name=PAF-AH 30 kDa subunit
PAF-AH subunit beta
Short name=PAFAH subunit beta

Gene names

Name:	PAFAH1B2
Synonyms:	PAFAHB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.
Catalytic activity	1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Subunit structure	Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.
Subcellular location	Cytoplasm.
Tissue specificity	Ubiquitous. Ref.1
Sequence similarities	Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Traceable author statement. Source: UniProtKB
Molecular function	1-alkyl-2-acetylglycerophosphocholine esterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 229

229

Platelet-activating factor acetylhydrolase IB subunit beta

PRO_0000058151

Sites

Active site

48

1

By similarity

Active site

193

1

By similarity

Active site

196

1

By similarity

Amino acid modifications

Modified residue

2

1

Phosphoserine Ref.4

Secondary structure

1

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229

Helix Strand Turn

Details...

Helix

8 – 10

3

Beta strand

19 – 21

3

Helix

23 – 37

15

Beta strand

41 – 47

7

Helix

48 – 53

6

Helix

57 – 62

6

Helix

64 – 66

3

Beta strand

68 – 72

5

Helix

78 – 86

9

Turn

87 – 90

4

Beta strand

96 – 101

6

Helix

111 – 128

18

Beta strand

133 – 137

5

Beta strand

143 – 145

3

Helix

148 – 163

16

Beta strand

164 – 167

4

Beta strand

170 – 173

4

Turn

188 – 190

3

Beta strand

194 – 197

4

Helix

199 – 219

21

Sequences

	Sequence		Length	Mass (Da)
	P68402 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 14CF5D48621AA504	FASTA	229	25,569
	10 20 30 40 50 60 MSQGDSNPAA IPHAAEDIQG DDRWMSQHNR FVLDCKDKEP DVLFVGDSMV QLMQQYEIWR 70 80 90 100 110 120 ELFSPLHALN FGIGGDTTRH VLWRLKNGEL ENIKPKVIVV WVGTNNHENT AEEVAGGIEA 130 140 150 160 170 180 IVQLINTRQP QAKIIVLGLL PRGEKPNPLR QKNAKVNQLL KVSLPKLANV QLLDTDGGFV 190 200 210 220 HSDGAISCHD MFDFLHLTGG GYAKICKPLH ELIMQLLEET PEEKQTTIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential tissue distribution of the beta- and gamma-subunits of human cytosolic platelet-activating factor acetylhydrolase (isoform I)." Adachi H., Tsujimoto M., Hattori M., Arai H., Inoue K. Biochem. Biophys. Res. Commun. 233:10-13(1997) [PubMed: 9144386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Fetal liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	Lubec G., Afjehi-Sadat L. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 61-79, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[4]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D63390 mRNA. Translation: BAA19917.1.
BC000398 mRNA. Translation: AAH00398.1.
BC019301 mRNA. Translation: AAH19301.1.

IPI

IPI00026546.

PIR

JC5409.

RefSeq

NP_001171677.1. NM_001184748.1.
NP_002563.1. NM_002572.3.

UniGene

Hs.597488.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VYH	X-ray	3.40	A/B/E/F/I/J/M/N/Q/R	1-229	[»]

ProteinModelPortal

P68402.

SMR

P68402. Positions 6-217.

ModBase

Search...

Protein-protein interaction databases

IntAct

P68402. 14 interactions.

MINT

MINT-5002700.

STRING

P68402.

PTM databases

PhosphoSite

P68402.

Polymorphism databases

DMDM

55977294.

2D gel databases

REPRODUCTION-2DPAGE

IPI00026546.

Proteomic databases

PeptideAtlas

P68402.

PRIDE

P68402.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000304808; ENSP00000304006; ENSG00000168092.

GeneID

5049.

KEGG

hsa:5049.

UCSC

uc001pqe.1. human.

Organism-specific databases

CTD

5049.

GeneCards

GC11P117048.

H-InvDB

HIX0010160.
HIX0022939.

HGNC

HGNC:8575. PAFAH1B2.

MIM

602508. gene.

neXtProt

NX_P68402.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG09501.

HOGENOM

HBG715888.

HOVERGEN

HBG053477.

InParanoid

P68402.

OMA

RFVQECK.

PhylomeDB

P68402.

Enzyme and pathway databases

Pathway_Interaction_DB

lis1pathway. Lissencephaly gene (LIS1) in neuronal migration and development.

Gene expression databases

ArrayExpress

P68402.

Bgee

P68402.

CleanEx

HS_PAFAH1B2.

Genevestigator

P68402.

GermOnline

ENSG00000168092. Homo sapiens.

Family and domain databases

InterPro

IPR013830. Esterase_SGNH_hydro-type.
IPR013831. Esterase_SGNH_hydro-type_subgr.
IPR001087. Lipase_GDSL.
[Graphical view]

Gene3D

G3DSA:3.40.50.1110. Esterase_SGNH_hydro-type_subgr. 1 hit.

KO

K01062.

Pfam

PF00657. Lipase_GDSL. 1 hit.
[Graphical view]

SUPFAM

SSF52266. Esterase_SGNH_hydro-type. 1 hit.

PROSITE

PS01098. LIPASE_GDSL_SER. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

19456.

SOURCE

Search...

Entry information

Entry name

PA1B2_HUMAN

Accession

Primary (citable) accession number: P68402
Secondary accession number(s): O00687, Q29459

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.