Platelet-activating factor acetylhydrolase IB subunit beta

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P68401 (PA1B2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 67. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Platelet-activating factor acetylhydrolase IB subunit beta
EC=3.1.1.47

Alternative name(s):
PAF acetylhydrolase 30 kDa subunit
Short name=PAF-AH 30 kDa subunit
PAF-AH subunit beta
Short name=PAFAH subunit beta

Gene names

Name:	PAFAH1B2
Synonyms:	PAFAHB

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.
Catalytic activity	1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H₂O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Subunit structure	Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of macroautophagy Inferred from electronic annotation. Source: Compara spermatogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	1-alkyl-2-acetylglycerophosphocholine esterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 229

228

Platelet-activating factor acetylhydrolase IB subunit beta

PRO_0000058149

Sites

Active site

By similarity

Active site

193

By similarity

Active site

196

By similarity

Amino acid modifications

Modified residue

N-acetylserine By similarity

Modified residue

Phosphoserine By similarity

Secondary structure

229

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P68401 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 14CF5D48621AA504
Show »

FASTA

229

25,569

        10         20         30         40         50         60 
MSQGDSNPAA IPHAAEDIQG DDRWMSQHNR FVLDCKDKEP DVLFVGDSMV QLMQQYEIWR 

        70         80         90        100        110        120 
ELFSPLHALN FGIGGDTTRH VLWRLKNGEL ENIKPKVIVV WVGTNNHENT AEEVAGGIEA 

       130        140        150        160        170        180 
IVQLINTRQP QAKIIVLGLL PRGEKPNPLR QKNAKVNQLL KVSLPKLANV QLLDTDGGFV 

       190        200        210        220 
HSDGAISCHD MFDFLHLTGG GYAKICKPLH ELIMQLLEET PEEKQTTIA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a cDNA encoding the beta-subunit (30-kDa subunit) of bovine brain platelet-activating factor acetylhydrolase." Hattori M., Adachi H., Aoki J., Tsujimoto M., Arai H., Inoue K. J. Biol. Chem. 270:31345-31352(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-53; 134-152 AND 167-196. Tissue: Brain.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.
[3]	"Preparation and crystal structure of the recombinant alpha(1)/alpha(2) catalytic heterodimer of bovine brain platelet-activating factor acetylhydrolase Ib." Sheffield P.J., McMullen T.W., Li J., Ho Y.S., Garrard S.M., Derewenda U., Derewenda Z.S. Protein Eng. 14:513-519(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS). Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D49678 mRNA. Translation: BAA08534.1.
BC103452 mRNA. Translation: AAI03453.1.

IPI

IPI00692295.

RefSeq

NP_777089.1. NM_174664.2.

UniGene

Bt.49728.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FXW	X-ray	2.10	F	1-229	[»]

ProteinModelPortal

P68401.

ModBase

Search...

Protein-protein interaction databases

IntAct

P68401. 1 interaction.

Proteomic databases

PaxDb

P68401.

PRIDE

P68401.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSBTAT00000007398; ENSBTAP00000007398; ENSBTAG00000005627.

GeneID

282514.

KEGG

bta:282514.

Organism-specific databases

CTD

5049.

Phylogenomic databases

eggNOG

NOG69837.

GeneTree

ENSGT00390000016520.

HOGENOM

HOG000232143.

HOVERGEN

HBG053477.

InParanoid

P68401.

K16795.

OMA

LQQYEIW.

OrthoDB

EOG45QHF4.

Family and domain databases

Gene3D

3.40.50.1110. 1 hit.

InterPro

IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]

PROSITE

PS01098. LIPASE_GDSL_SER. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P68401.

NextBio

20806264.

Entry information

Entry name

PA1B2_BOVIN

Accession

Primary (citable) accession number: P68401
Secondary accession number(s): O00687, Q29459, Q3ZBB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents