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P68401

- PA1B2_BOVIN

UniProt

P68401 - PA1B2_BOVIN

Protein

Platelet-activating factor acetylhydrolase IB subunit beta

Gene

PAFAH1B2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    • Comment

    Functioni

    Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.

    Catalytic activityi

    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei48 – 481By similarity
    Active sitei193 – 1931By similarity
    Active sitei196 – 1961By similarity

    GO - Molecular functioni

    1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. positive regulation of macroautophagy Source: Ensembl
    3. spermatogenesis Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet-activating factor acetylhydrolase IB subunit beta (EC:3.1.1.47)
    Alternative name(s):
    PAF acetylhydrolase 30 kDa subunit
    Short name:
    PAF-AH 30 kDa subunit
    PAF-AH subunit beta
    Short name:
    PAFAH subunit beta
    Gene namesi
    Name:PAFAH1B2
    Synonyms:PAFAHB
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 15

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleolus Source: Ensembl
    3. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 229228Platelet-activating factor acetylhydrolase IB subunit betaPRO_0000058149Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP68401.
    PRIDEiP68401.

    Interactioni

    Subunit structurei

    Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

    Protein-protein interaction databases

    IntActiP68401. 1 interaction.

    Structurei

    Secondary structure

    1
    229
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 103
    Beta strandi18 – 214
    Helixi23 – 3715
    Beta strandi41 – 477
    Helixi48 – 514
    Helixi52 – 554
    Helixi57 – 626
    Helixi64 – 663
    Beta strandi68 – 725
    Helixi78 – 869
    Turni87 – 904
    Beta strandi96 – 1016
    Helixi111 – 12818
    Beta strandi133 – 1375
    Beta strandi143 – 1453
    Helixi148 – 16316
    Beta strandi164 – 17310
    Turni188 – 1903
    Beta strandi194 – 1974
    Helixi199 – 21618

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FXWX-ray2.10F1-229[»]
    ProteinModelPortaliP68401.
    SMRiP68401. Positions 6-217.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP68401.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG69837.
    HOGENOMiHOG000232143.
    HOVERGENiHBG053477.
    InParanoidiP68401.
    KOiK16795.
    OMAiNIRPKVV.
    OrthoDBiEOG7HB5BS.
    TreeFamiTF323955.

    Family and domain databases

    Gene3Di3.40.50.1110. 1 hit.
    InterProiIPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P68401-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQGDSNPAA IPHAAEDIQG DDRWMSQHNR FVLDCKDKEP DVLFVGDSMV    50
    QLMQQYEIWR ELFSPLHALN FGIGGDTTRH VLWRLKNGEL ENIKPKVIVV 100
    WVGTNNHENT AEEVAGGIEA IVQLINTRQP QAKIIVLGLL PRGEKPNPLR 150
    QKNAKVNQLL KVSLPKLANV QLLDTDGGFV HSDGAISCHD MFDFLHLTGG 200
    GYAKICKPLH ELIMQLLEET PEEKQTTIA 229
    Length:229
    Mass (Da):25,569
    Last modified:November 23, 2004 - v1
    Checksum:i14CF5D48621AA504
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D49678 mRNA. Translation: BAA08534.1.
    BC103452 mRNA. Translation: AAI03453.1.
    RefSeqiNP_777089.1. NM_174664.2.
    XP_005215864.1. XM_005215807.1.
    UniGeneiBt.49728.

    Genome annotation databases

    EnsembliENSBTAT00000007398; ENSBTAP00000007398; ENSBTAG00000005627.
    GeneIDi282514.
    KEGGibta:282514.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D49678 mRNA. Translation: BAA08534.1 .
    BC103452 mRNA. Translation: AAI03453.1 .
    RefSeqi NP_777089.1. NM_174664.2.
    XP_005215864.1. XM_005215807.1.
    UniGenei Bt.49728.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FXW X-ray 2.10 F 1-229 [» ]
    ProteinModelPortali P68401.
    SMRi P68401. Positions 6-217.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P68401. 1 interaction.

    Proteomic databases

    PaxDbi P68401.
    PRIDEi P68401.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000007398 ; ENSBTAP00000007398 ; ENSBTAG00000005627 .
    GeneIDi 282514.
    KEGGi bta:282514.

    Organism-specific databases

    CTDi 5049.

    Phylogenomic databases

    eggNOGi NOG69837.
    HOGENOMi HOG000232143.
    HOVERGENi HBG053477.
    InParanoidi P68401.
    KOi K16795.
    OMAi NIRPKVV.
    OrthoDBi EOG7HB5BS.
    TreeFami TF323955.

    Miscellaneous databases

    EvolutionaryTracei P68401.
    NextBioi 20806264.

    Family and domain databases

    Gene3Di 3.40.50.1110. 1 hit.
    InterProi IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a cDNA encoding the beta-subunit (30-kDa subunit) of bovine brain platelet-activating factor acetylhydrolase."
      Hattori M., Adachi H., Aoki J., Tsujimoto M., Arai H., Inoue K.
      J. Biol. Chem. 270:31345-31352(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-53; 134-152 AND 167-196.
      Tissue: Brain.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Hypothalamus.
    3. "Preparation and crystal structure of the recombinant alpha(1)/alpha(2) catalytic heterodimer of bovine brain platelet-activating factor acetylhydrolase Ib."
      Sheffield P.J., McMullen T.W., Li J., Ho Y.S., Garrard S.M., Derewenda U., Derewenda Z.S.
      Protein Eng. 14:513-519(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
      Tissue: Brain.

    Entry informationi

    Entry nameiPA1B2_BOVIN
    AccessioniPrimary (citable) accession number: P68401
    Secondary accession number(s): O00687, Q29459, Q3ZBB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3