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P68401

- PA1B2_BOVIN

UniProt

P68401 - PA1B2_BOVIN

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Protein

Platelet-activating factor acetylhydrolase IB subunit beta

Gene

PAFAH1B2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.

Catalytic activityi

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei48 – 481By similarity
Active sitei193 – 1931By similarity
Active sitei196 – 1961By similarity

GO - Molecular functioni

  1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. positive regulation of macroautophagy Source: Ensembl
  3. spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase IB subunit beta (EC:3.1.1.47)
Alternative name(s):
PAF acetylhydrolase 30 kDa subunit
Short name:
PAF-AH 30 kDa subunit
PAF-AH subunit beta
Short name:
PAFAH subunit beta
Gene namesi
Name:PAFAH1B2
Synonyms:PAFAHB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 15

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. nucleolus Source: Ensembl
  4. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 229228Platelet-activating factor acetylhydrolase IB subunit betaPRO_0000058149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP68401.
PRIDEiP68401.

Interactioni

Subunit structurei

Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

Protein-protein interaction databases

IntActiP68401. 1 interaction.

Structurei

Secondary structure

1
229
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 103
Beta strandi18 – 214
Helixi23 – 3715
Beta strandi41 – 477
Helixi48 – 514
Helixi52 – 554
Helixi57 – 626
Helixi64 – 663
Beta strandi68 – 725
Helixi78 – 869
Turni87 – 904
Beta strandi96 – 1016
Helixi111 – 12818
Beta strandi133 – 1375
Beta strandi143 – 1453
Helixi148 – 16316
Beta strandi164 – 17310
Turni188 – 1903
Beta strandi194 – 1974
Helixi199 – 21618

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FXWX-ray2.10F1-229[»]
ProteinModelPortaliP68401.
SMRiP68401. Positions 6-217.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68401.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG69837.
HOGENOMiHOG000232143.
HOVERGENiHBG053477.
InParanoidiP68401.
KOiK16795.
OMAiNIRPKVV.
OrthoDBiEOG7HB5BS.
TreeFamiTF323955.

Family and domain databases

Gene3Di3.40.50.1110. 1 hit.
InterProiIPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68401-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQGDSNPAA IPHAAEDIQG DDRWMSQHNR FVLDCKDKEP DVLFVGDSMV
60 70 80 90 100
QLMQQYEIWR ELFSPLHALN FGIGGDTTRH VLWRLKNGEL ENIKPKVIVV
110 120 130 140 150
WVGTNNHENT AEEVAGGIEA IVQLINTRQP QAKIIVLGLL PRGEKPNPLR
160 170 180 190 200
QKNAKVNQLL KVSLPKLANV QLLDTDGGFV HSDGAISCHD MFDFLHLTGG
210 220
GYAKICKPLH ELIMQLLEET PEEKQTTIA
Length:229
Mass (Da):25,569
Last modified:November 23, 2004 - v1
Checksum:i14CF5D48621AA504
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D49678 mRNA. Translation: BAA08534.1.
BC103452 mRNA. Translation: AAI03453.1.
RefSeqiNP_777089.1. NM_174664.2.
XP_005215864.1. XM_005215807.1.
UniGeneiBt.49728.

Genome annotation databases

EnsembliENSBTAT00000007398; ENSBTAP00000007398; ENSBTAG00000005627.
GeneIDi282514.
KEGGibta:282514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D49678 mRNA. Translation: BAA08534.1 .
BC103452 mRNA. Translation: AAI03453.1 .
RefSeqi NP_777089.1. NM_174664.2.
XP_005215864.1. XM_005215807.1.
UniGenei Bt.49728.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FXW X-ray 2.10 F 1-229 [» ]
ProteinModelPortali P68401.
SMRi P68401. Positions 6-217.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P68401. 1 interaction.

Proteomic databases

PaxDbi P68401.
PRIDEi P68401.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000007398 ; ENSBTAP00000007398 ; ENSBTAG00000005627 .
GeneIDi 282514.
KEGGi bta:282514.

Organism-specific databases

CTDi 5049.

Phylogenomic databases

eggNOGi NOG69837.
HOGENOMi HOG000232143.
HOVERGENi HBG053477.
InParanoidi P68401.
KOi K16795.
OMAi NIRPKVV.
OrthoDBi EOG7HB5BS.
TreeFami TF323955.

Miscellaneous databases

EvolutionaryTracei P68401.
NextBioi 20806264.

Family and domain databases

Gene3Di 3.40.50.1110. 1 hit.
InterProi IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a cDNA encoding the beta-subunit (30-kDa subunit) of bovine brain platelet-activating factor acetylhydrolase."
    Hattori M., Adachi H., Aoki J., Tsujimoto M., Arai H., Inoue K.
    J. Biol. Chem. 270:31345-31352(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-53; 134-152 AND 167-196.
    Tissue: Brain.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.
  3. "Preparation and crystal structure of the recombinant alpha(1)/alpha(2) catalytic heterodimer of bovine brain platelet-activating factor acetylhydrolase Ib."
    Sheffield P.J., McMullen T.W., Li J., Ho Y.S., Garrard S.M., Derewenda U., Derewenda Z.S.
    Protein Eng. 14:513-519(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
    Tissue: Brain.

Entry informationi

Entry nameiPA1B2_BOVIN
AccessioniPrimary (citable) accession number: P68401
Secondary accession number(s): O00687, Q29459, Q3ZBB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3