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Protein

Platelet-activating factor acetylhydrolase IB subunit beta

Gene

PAFAH1B2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.

Catalytic activityi

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48By similarity1
Active sitei193By similarity1
Active sitei196By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.47. 908.
ReactomeiR-BTA-6798695. Neutrophil degranulation.
R-BTA-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Chemistry databases

SwissLipidsiSLP:000000692.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase IB subunit beta (EC:3.1.1.47)
Alternative name(s):
PAF acetylhydrolase 30 kDa subunit
Short name:
PAF-AH 30 kDa subunit
PAF-AH subunit beta
Short name:
PAFAH subunit beta
Gene namesi
Name:PAFAH1B2
Synonyms:PAFAHB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 15

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000581492 – 229Platelet-activating factor acetylhydrolase IB subunit betaAdd BLAST228

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei64PhosphoserineBy similarity1
Modified residuei220PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP68401.
PeptideAtlasiP68401.
PRIDEiP68401.

Expressioni

Gene expression databases

BgeeiENSBTAG00000005627.
ExpressionAtlasiP68401. differential.

Interactioni

Subunit structurei

Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

Protein-protein interaction databases

IntActiP68401. 1 interactor.
STRINGi9913.ENSBTAP00000007398.

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 10Combined sources3
Beta strandi18 – 21Combined sources4
Helixi23 – 37Combined sources15
Beta strandi41 – 47Combined sources7
Helixi48 – 51Combined sources4
Helixi52 – 55Combined sources4
Helixi57 – 62Combined sources6
Helixi64 – 66Combined sources3
Beta strandi68 – 72Combined sources5
Helixi78 – 86Combined sources9
Turni87 – 90Combined sources4
Beta strandi96 – 101Combined sources6
Helixi111 – 128Combined sources18
Beta strandi133 – 137Combined sources5
Beta strandi143 – 145Combined sources3
Helixi148 – 163Combined sources16
Beta strandi164 – 173Combined sources10
Turni188 – 190Combined sources3
Beta strandi194 – 197Combined sources4
Helixi199 – 216Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FXWX-ray2.10F1-229[»]
ProteinModelPortaliP68401.
SMRiP68401.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68401.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IMK6. Eukaryota.
ENOG410XPWQ. LUCA.
GeneTreeiENSGT00390000016520.
HOGENOMiHOG000232143.
HOVERGENiHBG053477.
InParanoidiP68401.
KOiK16795.
OMAiRGQQPNK.
OrthoDBiEOG091G0OII.
TreeFamiTF323955.

Family and domain databases

Gene3Di3.40.50.1110. 1 hit.
InterProiIPR013830. SGNH_hydro.
[Graphical view]
PfamiPF13472. Lipase_GDSL_2. 1 hit.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68401-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQGDSNPAA IPHAAEDIQG DDRWMSQHNR FVLDCKDKEP DVLFVGDSMV
60 70 80 90 100
QLMQQYEIWR ELFSPLHALN FGIGGDTTRH VLWRLKNGEL ENIKPKVIVV
110 120 130 140 150
WVGTNNHENT AEEVAGGIEA IVQLINTRQP QAKIIVLGLL PRGEKPNPLR
160 170 180 190 200
QKNAKVNQLL KVSLPKLANV QLLDTDGGFV HSDGAISCHD MFDFLHLTGG
210 220
GYAKICKPLH ELIMQLLEET PEEKQTTIA
Length:229
Mass (Da):25,569
Last modified:November 23, 2004 - v1
Checksum:i14CF5D48621AA504
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49678 mRNA. Translation: BAA08534.1.
BC103452 mRNA. Translation: AAI03453.1.
RefSeqiNP_777089.1. NM_174664.2.
UniGeneiBt.49728.

Genome annotation databases

EnsembliENSBTAT00000007398; ENSBTAP00000007398; ENSBTAG00000005627.
GeneIDi282514.
KEGGibta:282514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49678 mRNA. Translation: BAA08534.1.
BC103452 mRNA. Translation: AAI03453.1.
RefSeqiNP_777089.1. NM_174664.2.
UniGeneiBt.49728.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FXWX-ray2.10F1-229[»]
ProteinModelPortaliP68401.
SMRiP68401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP68401. 1 interactor.
STRINGi9913.ENSBTAP00000007398.

Chemistry databases

SwissLipidsiSLP:000000692.

Proteomic databases

PaxDbiP68401.
PeptideAtlasiP68401.
PRIDEiP68401.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007398; ENSBTAP00000007398; ENSBTAG00000005627.
GeneIDi282514.
KEGGibta:282514.

Organism-specific databases

CTDi5049.

Phylogenomic databases

eggNOGiENOG410IMK6. Eukaryota.
ENOG410XPWQ. LUCA.
GeneTreeiENSGT00390000016520.
HOGENOMiHOG000232143.
HOVERGENiHBG053477.
InParanoidiP68401.
KOiK16795.
OMAiRGQQPNK.
OrthoDBiEOG091G0OII.
TreeFamiTF323955.

Enzyme and pathway databases

BRENDAi3.1.1.47. 908.
ReactomeiR-BTA-6798695. Neutrophil degranulation.
R-BTA-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Miscellaneous databases

EvolutionaryTraceiP68401.

Gene expression databases

BgeeiENSBTAG00000005627.
ExpressionAtlasiP68401. differential.

Family and domain databases

Gene3Di3.40.50.1110. 1 hit.
InterProiIPR013830. SGNH_hydro.
[Graphical view]
PfamiPF13472. Lipase_GDSL_2. 1 hit.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPA1B2_BOVIN
AccessioniPrimary (citable) accession number: P68401
Secondary accession number(s): O00687, Q29459, Q3ZBB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.