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Protein

tRNA-specific adenosine deaminase

Gene

tadA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2). Essential for cell viability.UniRule annotation2 Publications

Catalytic activityi

Adenine(34) in tRNA + H2O = hypoxanthine(34) in tRNA + NH3.UniRule annotation2 Publications

Cofactori

Zn2+UniRule annotation1 PublicationNote: Binds 1 zinc ion per subunit.UniRule annotation1 Publication

Kineticsi

kcat is 13 min(-1).1 Publication

Manual assertion based on experiment ini

  1. KM=0.83 µM for tRNA(Arg2)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi57Zinc; via pros nitrogen; catalyticUniRule annotation1 Publication1
    Active sitei59Proton donor1 Publication1
    Metal bindingi87Zinc; catalyticUniRule annotation1 Publication1
    Metal bindingi90Zinc; catalyticUniRule annotation1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    • tRNA wobble adenosine to inosine editing Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11372-MONOMER.
    ECOL316407:JW2543-MONOMER.
    MetaCyc:EG11372-MONOMER.
    BRENDAi3.5.4.33. 2026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-specific adenosine deaminaseUniRule annotation (EC:3.5.4.33UniRule annotation)
    Gene namesi
    Name:tadAUniRule annotation
    Synonyms:yfhC
    Ordered Locus Names:b2559, JW2543
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11372. tadA.

    Pathology & Biotechi

    Disruption phenotypei

    Mutation makes E.coli resistant to the toxic proteins encoded by the gef gene family.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi53D → E: Resistance to the cell-killing function encoded by the gef gene family. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001717331 – 167tRNA-specific adenosine deaminaseAdd BLAST167

    Proteomic databases

    PaxDbiP68398.
    PRIDEiP68398.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation2 Publications

    Protein-protein interaction databases

    BioGridi4261245. 24 interactors.
    IntActiP68398. 4 interactors.
    STRINGi511145.b2559.

    Structurei

    Secondary structure

    1167
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi8 – 24Combined sources17
    Beta strandi31 – 36Combined sources6
    Beta strandi39 – 45Combined sources7
    Helixi48 – 51Combined sources4
    Helixi58 – 70Combined sources13
    Beta strandi79 – 84Combined sources6
    Helixi88 – 97Combined sources10
    Beta strandi100 – 106Combined sources7
    Turni109 – 111Combined sources3
    Beta strandi131 – 133Combined sources3
    Helixi138 – 153Combined sources16

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1Z3AX-ray2.03A/B2-167[»]
    ProteinModelPortaliP68398.
    SMRiP68398.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP68398.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini6 – 117CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST112

    Sequence similaritiesi

    Belongs to the cytidine and deoxycytidylate deaminase family.UniRule annotation
    Contains 1 CMP/dCMP-type deaminase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiENOG4108Z6B. Bacteria.
    COG0590. LUCA.
    HOGENOMiHOG000085050.
    InParanoidiP68398.
    KOiK11991.
    OMAiRIGRIFF.
    PhylomeDBiP68398.

    Family and domain databases

    HAMAPiMF_00972. tRNA_aden_deaminase. 1 hit.
    InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR002125. CMP_dCMP_Zn-bd.
    IPR016193. Cytidine_deaminase-like.
    IPR028883. tRNA_aden_deaminase.
    [Graphical view]
    PfamiPF14437. MafB19-deam. 1 hit.
    [Graphical view]
    SUPFAMiSSF53927. SSF53927. 1 hit.
    PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
    PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P68398-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSEVEFSHEY WMRHALTLAK RAWDEREVPV GAVLVHNNRV IGEGWNRPIG
    60 70 80 90 100
    RHDPTAHAEI MALRQGGLVM QNYRLIDATL YVTLEPCVMC AGAMIHSRIG
    110 120 130 140 150
    RVVFGARDAK TGAAGSLMDV LHHPGMNHRV EITEGILADE CAALLSDFFR
    160
    MRRQEIKAQK KAQSSTD
    Length:167
    Mass (Da):18,718
    Last modified:June 26, 2007 - v2
    Checksum:i6EB59445A86964BC
    GO

    Sequence cautioni

    The sequence AAA79821 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAA10909 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAE76735 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence CAA51064 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X72336 Genomic DNA. Translation: CAA51064.1. Different initiation.
    D64044 Genomic DNA. Translation: BAA10909.1. Different initiation.
    U36841 Genomic DNA. Translation: AAA79821.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC75612.2.
    AP009048 Genomic DNA. Translation: BAE76735.1. Different initiation.
    PIRiF65033.
    RefSeqiNP_417054.2. NC_000913.3.
    WP_001297409.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75612; AAC75612; b2559.
    BAE76735; BAE76735; BAE76735.
    GeneIDi947027.
    KEGGiecj:JW2543.
    eco:b2559.
    PATRICi32120517. VBIEscCol129921_2661.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X72336 Genomic DNA. Translation: CAA51064.1. Different initiation.
    D64044 Genomic DNA. Translation: BAA10909.1. Different initiation.
    U36841 Genomic DNA. Translation: AAA79821.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC75612.2.
    AP009048 Genomic DNA. Translation: BAE76735.1. Different initiation.
    PIRiF65033.
    RefSeqiNP_417054.2. NC_000913.3.
    WP_001297409.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1Z3AX-ray2.03A/B2-167[»]
    ProteinModelPortaliP68398.
    SMRiP68398.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261245. 24 interactors.
    IntActiP68398. 4 interactors.
    STRINGi511145.b2559.

    Proteomic databases

    PaxDbiP68398.
    PRIDEiP68398.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75612; AAC75612; b2559.
    BAE76735; BAE76735; BAE76735.
    GeneIDi947027.
    KEGGiecj:JW2543.
    eco:b2559.
    PATRICi32120517. VBIEscCol129921_2661.

    Organism-specific databases

    EchoBASEiEB1346.
    EcoGeneiEG11372. tadA.

    Phylogenomic databases

    eggNOGiENOG4108Z6B. Bacteria.
    COG0590. LUCA.
    HOGENOMiHOG000085050.
    InParanoidiP68398.
    KOiK11991.
    OMAiRIGRIFF.
    PhylomeDBiP68398.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11372-MONOMER.
    ECOL316407:JW2543-MONOMER.
    MetaCyc:EG11372-MONOMER.
    BRENDAi3.5.4.33. 2026.

    Miscellaneous databases

    EvolutionaryTraceiP68398.
    PROiP68398.

    Family and domain databases

    HAMAPiMF_00972. tRNA_aden_deaminase. 1 hit.
    InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR002125. CMP_dCMP_Zn-bd.
    IPR016193. Cytidine_deaminase-like.
    IPR028883. tRNA_aden_deaminase.
    [Graphical view]
    PfamiPF14437. MafB19-deam. 1 hit.
    [Graphical view]
    SUPFAMiSSF53927. SSF53927. 1 hit.
    PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
    PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiTADA_ECOLI
    AccessioniPrimary (citable) accession number: P68398
    Secondary accession number(s): P30134, Q2MAH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: June 26, 2007
    Last modified: November 2, 2016
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.