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Protein

tRNA-specific adenosine deaminase

Gene

tadA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2). Essential for cell viability.UniRule annotation2 Publications

Catalytic activityi

Adenine(34) in tRNA + H2O = hypoxanthine(34) in tRNA + NH3.UniRule annotation2 Publications

Cofactori

Zn2+UniRule annotation1 PublicationNote: Binds 1 zinc ion per subunit.UniRule annotation1 Publication

Kineticsi

kcat is 13 min(-1).1 Publication
  1. KM=0.83 µM for tRNA(Arg2)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi57Zinc; via pros nitrogen; catalyticUniRule annotation1 Publication1
    Active sitei59Proton donor1 Publication1
    Metal bindingi87Zinc; catalyticUniRule annotation1 Publication1
    Metal bindingi90Zinc; catalyticUniRule annotation1 Publication1

    GO - Molecular functioni

    • tRNA-specific adenosine-34 deaminase activity Source: EcoCyc
    • zinc ion binding Source: EcoCyc

    GO - Biological processi

    • tRNA wobble adenosine to inosine editing Source: EcoCyc

    Keywordsi

    Molecular functionHydrolase
    Biological processtRNA processing
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11372-MONOMER
    MetaCyc:EG11372-MONOMER
    BRENDAi3.5.4.33 2026

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-specific adenosine deaminaseUniRule annotation (EC:3.5.4.33UniRule annotation)
    Gene namesi
    Name:tadAUniRule annotation
    Synonyms:yfhC
    Ordered Locus Names:b2559, JW2543
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11372 tadA

    Pathology & Biotechi

    Disruption phenotypei

    Mutation makes E.coli resistant to the toxic proteins encoded by the gef gene family.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi53D → E: Resistance to the cell-killing function encoded by the gef gene family. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001717331 – 167tRNA-specific adenosine deaminaseAdd BLAST167

    Proteomic databases

    PaxDbiP68398
    PRIDEiP68398

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation2 Publications

    Protein-protein interaction databases

    BioGridi4261245, 59 interactors
    IntActiP68398, 4 interactors
    STRINGi316385.ECDH10B_2727

    Structurei

    Secondary structure

    1167
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi8 – 24Combined sources17
    Beta strandi31 – 36Combined sources6
    Beta strandi39 – 45Combined sources7
    Helixi48 – 51Combined sources4
    Helixi58 – 70Combined sources13
    Beta strandi79 – 84Combined sources6
    Helixi88 – 97Combined sources10
    Beta strandi100 – 106Combined sources7
    Turni109 – 111Combined sources3
    Beta strandi131 – 133Combined sources3
    Helixi138 – 153Combined sources16

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1Z3AX-ray2.03A/B2-167[»]
    ProteinModelPortaliP68398
    SMRiP68398
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP68398

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini6 – 117CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST112

    Sequence similaritiesi

    Belongs to the cytidine and deoxycytidylate deaminase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4108Z6B Bacteria
    COG0590 LUCA
    HOGENOMiHOG000085050
    InParanoidiP68398
    KOiK11991
    OMAiLEPCQMC
    PhylomeDBiP68398

    Family and domain databases

    HAMAPiMF_00972 tRNA_aden_deaminase, 1 hit
    InterProiView protein in InterPro
    IPR016192 APOBEC/CMP_deaminase_Zn-bd
    IPR002125 CMP_dCMP_dom
    IPR016193 Cytidine_deaminase-like
    IPR028883 tRNA_aden_deaminase
    PfamiView protein in Pfam
    PF14437 MafB19-deam, 1 hit
    SUPFAMiSSF53927 SSF53927, 1 hit
    PROSITEiView protein in PROSITE
    PS00903 CYT_DCMP_DEAMINASES_1, 1 hit
    PS51747 CYT_DCMP_DEAMINASES_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P68398-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSEVEFSHEY WMRHALTLAK RAWDEREVPV GAVLVHNNRV IGEGWNRPIG
    60 70 80 90 100
    RHDPTAHAEI MALRQGGLVM QNYRLIDATL YVTLEPCVMC AGAMIHSRIG
    110 120 130 140 150
    RVVFGARDAK TGAAGSLMDV LHHPGMNHRV EITEGILADE CAALLSDFFR
    160
    MRRQEIKAQK KAQSSTD
    Length:167
    Mass (Da):18,718
    Last modified:June 26, 2007 - v2
    Checksum:i6EB59445A86964BC
    GO

    Sequence cautioni

    The sequence AAA79821 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAA10909 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAE76735 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence CAA51064 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X72336 Genomic DNA Translation: CAA51064.1 Different initiation.
    D64044 Genomic DNA Translation: BAA10909.1 Different initiation.
    U36841 Genomic DNA Translation: AAA79821.1 Different initiation.
    U00096 Genomic DNA Translation: AAC75612.2
    AP009048 Genomic DNA Translation: BAE76735.1 Different initiation.
    PIRiF65033
    RefSeqiNP_417054.2, NC_000913.3

    Genome annotation databases

    EnsemblBacteriaiAAC75612; AAC75612; b2559
    BAE76735; BAE76735; BAE76735
    GeneIDi947027
    KEGGiecj:JW2543
    eco:b2559
    PATRICifig|1411691.4.peg.4175

    Similar proteinsi

    Entry informationi

    Entry nameiTADA_ECOLI
    AccessioniPrimary (citable) accession number: P68398
    Secondary accession number(s): P30134, Q2MAH1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: June 26, 2007
    Last modified: March 28, 2018
    This is version 105 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

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