Reviewed,
UniProtKB/Swiss-Prot P68301 (MT2_BOVIN)
Last modified
November 24, 2009.
Version 32.
History...
Clusters with 100%,
90%,
50% identity |
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Metallothionein-2 Short name=MT-2 Alternative name(s): Metallothionein-II Short name=MT-II | ||||
| Gene names |
| ||||
| Organism | Bos taurus (Bovine) | ||||
| Taxonomic identifier | 9913 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 61 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. |
| Domain | Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines. |
| Sequence similarities | Belongs to the metallothionein superfamily. Type 1 family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Metal-binding Metal-thiolate cluster |
| PTM | Acetylation |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Molecular function | metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 61 | 61 | Metallothionein-2 | PRO_0000197225 | |||||
Regions | |||||||||
| Region | 1 – 29 | 29 | Beta | ||||||
| Region | 30 – 61 | 32 | Alpha | ||||||
Sites | |||||||||
| Metal binding | 5 | 1 | Cluster B | ||||||
| Metal binding | 7 | 1 | Cluster B | ||||||
| Metal binding | 13 | 1 | Cluster B | ||||||
| Metal binding | 15 | 1 | Cluster B | ||||||
| Metal binding | 19 | 1 | Cluster B | ||||||
| Metal binding | 21 | 1 | Cluster B | ||||||
| Metal binding | 24 | 1 | Cluster B | ||||||
| Metal binding | 26 | 1 | Cluster B | ||||||
| Metal binding | 29 | 1 | Cluster B | ||||||
| Metal binding | 33 | 1 | Cluster A | ||||||
| Metal binding | 34 | 1 | Cluster A | ||||||
| Metal binding | 36 | 1 | Cluster A | ||||||
| Metal binding | 37 | 1 | Cluster A | ||||||
| Metal binding | 41 | 1 | Cluster A | ||||||
| Metal binding | 44 | 1 | Cluster A | ||||||
| Metal binding | 48 | 1 | Cluster A | ||||||
| Metal binding | 50 | 1 | Cluster A | ||||||
| Metal binding | 57 | 1 | Cluster A | ||||||
| Metal binding | 59 | 1 | Cluster A | ||||||
| Metal binding | 60 | 1 | Cluster A | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine Ref.1 | ||||||
| Modified residue | 51 | 1 | N6-acetyllysine By similarity | ||||||
Sequences
References
| [1] | "(Cu,Zn)-metallothioneins from fetal bovine liver. Chemical and spectroscopic properties." Muenger K., Germann U.A., Beltramini M., Niedermann D., Baitella-Eberle G., Kaegi J.H.R., Lerch K. J. Biol. Chem. 260:10032-10038(1985) [PubMed: 4019500] [Abstract] Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1. Tissue: Liver. |
| [2] | "Bovine metallothioneins in Italian population." Salvini M., Conte G., Taddei S., Durante M. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
Cross-references
Sequence databases | |
|---|---|
| AJ489256 mRNA. Translation: CAD33256.1. | |
| IPI | IPI00706794. |
| PIR | B23889. |
| RefSeq | XP_586929.3. |
| UniGene | Bt.2529 |
3D structure databases | |
| SMR | P68301. Positions 1-61. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P68301. |
Proteomic databases | |
| PRIDE | P68301. |
Genome annotation databases | |
| Ensembl | ENSBTAT00000034373; ENSBTAP00000034269; ENSBTAG00000023659; Bos taurus. [Genome view] |
| GeneID | 404070. |
| KEGG | bta:404070. |
Organism-specific databases | |
| CTD | 404070. |
Phylogenomic databases | |
| HOVERGEN | P68301. |
| OMA | SQGCICK |
Family and domain databases | |
| InterPro | IPR017854. Metallothionein_sfam. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. IPR018064. Metallothionein_vert_metal_BS. [Graphical view] |
| Gene3D | G3DSA:4.10.10.10. Metallothionein_vert. 1 hit. |
| PANTHER | PTHR23299. Metallothionein_vert. 1 hit. |
| Pfam | PF00131. Metallothio. 1 hit. [Graphical view] |
| PRINTS | PR00860. MTVERTEBRATE. |
| PROSITE | PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MT2_BOVIN | ||||||||
| Accession | Primary (citable) accession number: P68301 Secondary accession number(s): P09579, Q546G1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Metallothioneins Classification of metallothioneins and list of entries |
| SIMILARITY comments Index of protein domains and families |
