P68301 (MT2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metallothionein-2
Short name=MT-2

Alternative name(s):
Metallothionein-II
Short name=MT-II

Gene names

Name:	MT2
Synonyms:	MT2A

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of growth Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Metallothionein-2		PRO_0000197225
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Divalent metal cation; cluster B
	Metal binding	7	1	Divalent metal cation; cluster B
	Metal binding	13	1	Divalent metal cation; cluster B
	Metal binding	15	1	Divalent metal cation; cluster B
	Metal binding	19	1	Divalent metal cation; cluster B
	Metal binding	21	1	Divalent metal cation; cluster B
	Metal binding	24	1	Divalent metal cation; cluster B
	Metal binding	26	1	Divalent metal cation; cluster B
	Metal binding	29	1	Divalent metal cation; cluster B
	Metal binding	33	1	Divalent metal cation; cluster A
	Metal binding	34	1	Divalent metal cation; cluster A
	Metal binding	36	1	Divalent metal cation; cluster A
	Metal binding	37	1	Divalent metal cation; cluster A
	Metal binding	41	1	Divalent metal cation; cluster A
	Metal binding	44	1	Divalent metal cation; cluster A
	Metal binding	48	1	Divalent metal cation; cluster A
	Metal binding	50	1	Divalent metal cation; cluster A
	Metal binding	57	1	Divalent metal cation; cluster A
	Metal binding	59	1	Divalent metal cation; cluster A
	Metal binding	60	1	Divalent metal cation; cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.1
	Modified residue	51	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68301 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 7B0A626F8121F1D3	FASTA	61	6,028
	10 20 30 40 50 60 MDPNCSCTAG ESCTCAGSCK CKDCKCASCK KSCCSCCPVG CAKCAQGCVC KGASDKCSCC A

References

[1]	"(Cu,Zn)-metallothioneins from fetal bovine liver. Chemical and spectroscopic properties." Muenger K., Germann U.A., Beltramini M., Niedermann D., Baitella-Eberle G., Kaegi J.H.R., Lerch K. J. Biol. Chem. 260:10032-10038(1985) [PubMed: 4019500] [Abstract] Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1. Tissue: Liver.
[2]	"Bovine metallothioneins in Italian population." Salvini M., Conte G., Taddei S., Durante M. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ489256 mRNA. Translation: CAD33256.1.
IPI	IPI00706794.
PIR	B23889.
RefSeq	NP_001068608.1. NM_001075140.1.
UniGene	Bt.2529.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P68301.
Proteomic databases
PRIDE	P68301.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000034373; ENSBTAP00000034269; ENSBTAG00000023659.
GeneID	404070.
KEGG	bta:404070.
Organism-specific databases
CTD	4502.
Phylogenomic databases
InParanoid	P68301.
OMA	NCAGSCK.
OrthoDB	EOG46WZB6.
Family and domain databases
InterPro	IPR017854. Metalthion_dom. IPR023587. Metalthion_dom_vert. IPR003019. Metalthion_sfam_euk. IPR000006. Metalthion_vert. IPR018064. Metalthion_vert_metal_BS. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
KO	K14739.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
SUPFAM	SSF57868. Metallothionein_sfam. 1 hit.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MT2_BOVIN

Accession

Primary (citable) accession number: P68301
Secondary accession number(s): P09579, Q546G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program