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Protein

14-3-3 protein theta

Gene

Ywhaq

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei56 – 561Interaction with phosphoserine on interacting proteinBy similarity
Sitei127 – 1271Interaction with phosphoserine on interacting proteinBy similarity

GO - Molecular functioni

  1. 14-3-3 protein binding Source: RGD
  2. ion channel binding Source: RGD
  3. protein C-terminus binding Source: RGD
  4. protein N-terminus binding Source: RGD

GO - Biological processi

  1. negative regulation of ion transmembrane transport Source: RGD
  2. protein targeting Source: Ensembl
  3. small GTPase mediated signal transduction Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein theta
Alternative name(s):
14-3-3 protein tau
Gene namesi
Name:Ywhaq
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi3979. Ywhaq.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24524514-3-3 protein thetaPRO_0000058640Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei3 – 31N6-acetyllysineBy similarity
Modified residuei49 – 491N6-acetyllysineBy similarity
Modified residuei68 – 681N6-acetyllysineBy similarity
Modified residuei82 – 821Nitrated tyrosineBy similarity
Modified residuei104 – 1041Nitrated tyrosineBy similarity
Modified residuei115 – 1151N6-acetyllysineBy similarity
Modified residuei232 – 2321Phosphoserine; by CK1Curated

Keywords - PTMi

Acetylation, Nitration, Phosphoprotein

Proteomic databases

PaxDbiP68255.
PRIDEiP68255.

PTM databases

PhosphoSiteiP68255.

Expressioni

Gene expression databases

GenevestigatoriP68255.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with CDK16. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 (By similarity). Interacts with GAB2 (By similarity). Interacts with the 'Ser-241' phosphorylated form of PDPK1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi247608. 4 interactions.
DIPiDIP-947N.
IntActiP68255. 5 interactions.
MINTiMINT-203396.

Structurei

3D structure databases

SMRiP68255. Positions 1-230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiCOG5040.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP68255.
KOiK16197.
OMAiSGDDRKQ.
OrthoDBiEOG7HHWT3.
PhylomeDBiP68255.
TreeFamiTF102002.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN
60 70 80 90 100
VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL
110 120 130 140 150
LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ
160 170 180 190 200
EAFDISKKEM QPTHPIRLGL ALNFSVFYYE ILNNPELACT LAKTAFDEAI
210 220 230 240
AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN
Length:245
Mass (Da):27,778
Last modified:October 25, 2004 - v1
Checksum:iE30471260E8B366E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17614 mRNA. Translation: BAA04533.1.
BC062409 mRNA. Translation: AAH62409.1.
PIRiI52647. S59927.
RefSeqiNP_037185.1. NM_013053.1.
UniGeneiRn.2502.

Genome annotation databases

EnsembliENSRNOT00000011501; ENSRNOP00000011501; ENSRNOG00000008104.
GeneIDi25577.
KEGGirno:25577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17614 mRNA. Translation: BAA04533.1.
BC062409 mRNA. Translation: AAH62409.1.
PIRiI52647. S59927.
RefSeqiNP_037185.1. NM_013053.1.
UniGeneiRn.2502.

3D structure databases

SMRiP68255. Positions 1-230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247608. 4 interactions.
DIPiDIP-947N.
IntActiP68255. 5 interactions.
MINTiMINT-203396.

PTM databases

PhosphoSiteiP68255.

Proteomic databases

PaxDbiP68255.
PRIDEiP68255.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011501; ENSRNOP00000011501; ENSRNOG00000008104.
GeneIDi25577.
KEGGirno:25577.

Organism-specific databases

CTDi10971.
RGDi3979. Ywhaq.

Phylogenomic databases

eggNOGiCOG5040.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP68255.
KOiK16197.
OMAiSGDDRKQ.
OrthoDBiEOG7HHWT3.
PhylomeDBiP68255.
TreeFamiTF102002.

Miscellaneous databases

NextBioi607215.
PROiP68255.

Gene expression databases

GenevestigatoriP68255.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of rat cDNAs for the zeta and theta subtypes of 14-3-3 protein and differential distributions of their mRNAs in the brain."
    Watanabe M., Isobe T., Ichimura T., Kuwano R., Takahashi Y., Kondo H., Inoue Y.
    Brain Res. Mol. Brain Res. 25:113-121(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  3. Lubec G., Kang S.U., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 4-9; 12-18; 28-49; 61-68; 84-115; 128-157; 159-167 AND 194-222, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry namei1433T_RAT
AccessioniPrimary (citable) accession number: P68255
Secondary accession number(s): P35216
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: April 29, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.