P68255 (1433T_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3 protein theta

Alternative name(s):
14-3-3 protein tau

Gene names

Name:

Ywhaq

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 By similarity.
Subunit structure	Homodimer By similarity. Interacts with CDK16. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 By similarity. Interacts with GAB2 By similarity. Interacts with the 'Ser-241' phosphorylated form of PDPK1 By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell protein complex Inferred from direct assay PubMed 17457363. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	14-3-3 protein theta		PRO_0000058640
Sites
	Site	56	1	Interaction with phosphoserine on interacting protein By similarity
	Site	127	1	Interaction with phosphoserine on interacting protein By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	3	1	N6-acetyllysine By similarity
	Modified residue	49	1	N6-acetyllysine By similarity
	Modified residue	68	1	N6-acetyllysine By similarity
	Modified residue	115	1	N6-acetyllysine By similarity
	Modified residue	232	1	Phosphoserine; by CK1 Probable

Sequences

	Sequence		Length	Mass (Da)
	P68255 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: E30471260E8B366E	FASTA	245	27,778
	10 20 30 40 50 60 MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN VVGGRRSAWR 70 80 90 100 110 120 VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL LDKYLIANAT NPESKVFYLK 130 140 150 160 170 180 MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ EAFDISKKEM QPTHPIRLGL ALNFSVFYYE 190 200 210 220 230 240 ILNNPELACT LAKTAFDEAI AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of rat cDNAs for the zeta and theta subtypes of 14-3-3 protein and differential distributions of their mRNAs in the brain." Watanabe M., Isobe T., Ichimura T., Kuwano R., Takahashi Y., Kondo H., Inoue Y. Brain Res. Mol. Brain Res. 25:113-121(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[3]	Lubec G., Kang S.U., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 4-9; 12-18; 28-49; 61-68; 84-115; 128-157; 159-167 AND 194-222, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17614 mRNA. Translation: BAA04533.1. BC062409 mRNA. Translation: AAH62409.1.
IPI	IPI00196661.
PIR	S59927. I52647.
RefSeq	NP_037185.1. NM_013053.1.
UniGene	Rn.2502.
3D structure databases
ProteinModelPortal	P68255.
SMR	P68255. Positions 1-230.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-947N.
IntAct	P68255. 1 interaction.
MINT	MINT-203396.
PTM databases
PhosphoSite	P68255.
Proteomic databases
PaxDb	P68255.
PRIDE	P68255.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000011501; ENSRNOP00000011501; ENSRNOG00000008104.
GeneID	25577.
KEGG	rno:25577.
Organism-specific databases
CTD	10971.
RGD	3979. Ywhaq.
Phylogenomic databases
eggNOG	COG5040.
GeneTree	ENSGT00690000101991.
HOGENOM	HOG000240379.
HOVERGEN	HBG050423.
InParanoid	P68255.
KO	K16197.
OMA	AMEKTEL.
OrthoDB	EOG480HXJ.
Gene expression databases
ArrayExpress	P68255.
Genevestigator	P68255.
GermOnline	ENSRNOG00000008104. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.190.20. 1 hit.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607215.

Entry information

Entry name

1433T_RAT

Accession

Primary (citable) accession number: P68255
Secondary accession number(s): P35216

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program