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Protein

14-3-3 protein theta

Gene

Ywhaq

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei56 – 561Interaction with phosphoserine on interacting proteinBy similarity
Sitei127 – 1271Interaction with phosphoserine on interacting proteinBy similarity

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: Ensembl
  2. substantia nigra development Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_302653. Translocation of GLUT4 to the plasma membrane.
REACT_318722. Activation of BAD and translocation to mitochondria.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein theta
Alternative name(s):
14-3-3 protein tau
Gene namesi
Name:Ywhaq
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:891963. Ywhaq.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic vesicle membrane Source: Reactome
  2. cytosol Source: Reactome
  3. extracellular vesicular exosome Source: Ensembl
  4. focal adhesion Source: Ensembl
  5. protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24524514-3-3 protein thetaPRO_0000058637Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei3 – 31N6-acetyllysineBy similarity
Modified residuei49 – 491N6-acetyllysineBy similarity
Modified residuei68 – 681N6-acetyllysineBy similarity
Modified residuei82 – 821Nitrated tyrosineBy similarity
Modified residuei104 – 1041Nitrated tyrosineBy similarity
Modified residuei115 – 1151N6-acetyllysineBy similarity
Modified residuei232 – 2321Phosphoserine; by CK1Curated

Keywords - PTMi

Acetylation, Nitration, Phosphoprotein

Proteomic databases

MaxQBiP68254.
PaxDbiP68254.
PRIDEiP68254.

2D gel databases

REPRODUCTION-2DPAGEP68254.

PTM databases

PhosphoSiteiP68254.

Expressioni

Gene expression databases

BgeeiP68254.
ExpressionAtlasiP68254. baseline and differential.
GenevestigatoriP68254.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 (By similarity). Interacts with GAB2 (By similarity). Interacts with CDK16. Interacts with the 'Ser-241' phosphorylated form of PDPK1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Lrrk2Q5S0063EBI-400675,EBI-2693710

Protein-protein interaction databases

BioGridi204622. 11 interactions.
IntActiP68254. 13 interactions.
MINTiMINT-1520413.

Structurei

3D structure databases

SMRiP68254. Positions 1-230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiCOG5040.
GeneTreeiENSGT00760000119116.
HOVERGENiHBG050423.
InParanoidiP68254.
KOiK16197.
PhylomeDBiP68254.
TreeFamiTF102002.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P68254-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN
60 70 80 90 100
VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL
110 120 130 140 150
LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ
160 170 180 190 200
EAFDISKKEM QPTHPIRLGL ALNFSVFYYE ILNNPELACT LAKTAFDEAI
210 220 230 240
AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN
Length:245
Mass (Da):27,778
Last modified:October 24, 2004 - v1
Checksum:iE30471260E8B366E
GO
Isoform 2 (identifier: P68254-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     227-245: LWTSDSAGEECDAAEGAEN → FTCVELETVSVCFSLLS

Note: No experimental confirmation available.

Show »
Length:243
Mass (Da):27,701
Checksum:iE03CA8AFF03BA947
GO

Sequence cautioni

The sequence AAH80802.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH85299.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261M → I in BAE35397 (PubMed:16141072).Curated
Sequence conflicti227 – 2271L → S in BAE27120 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei227 – 24519LWTSD…EGAEN → FTCVELETVSVCFSLLS in isoform 2. 1 PublicationVSP_016340Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57312 mRNA. Translation: AAC53257.1.
U56243 mRNA. Translation: AAB72023.1.
D87662 mRNA. Translation: BAA13423.1.
AK145568 mRNA. Translation: BAE26517.1.
AK146371 mRNA. Translation: BAE27120.1.
AK150850 mRNA. Translation: BAE29907.1.
AK151437 mRNA. Translation: BAE30400.1.
AK151715 mRNA. Translation: BAE30634.1.
AK151864 mRNA. Translation: BAE30752.1.
AK159403 mRNA. Translation: BAE35055.1.
AK159817 mRNA. Translation: BAE35397.1.
AK163237 mRNA. Translation: BAE37249.1.
AK167397 mRNA. Translation: BAE39486.1.
AL929409 Genomic DNA. Translation: CAI25590.1.
BC080802 mRNA. Translation: AAH80802.1. Different initiation.
BC085299 mRNA. Translation: AAH85299.1. Different initiation.
BC090838 mRNA. Translation: AAH90838.1.
BC106164 mRNA. Translation: AAI06165.1.
CCDSiCCDS25837.1. [P68254-1]
RefSeqiNP_035869.1. NM_011739.3. [P68254-1]
UniGeneiMm.289630.

Genome annotation databases

EnsembliENSMUST00000049531; ENSMUSP00000106602; ENSMUSG00000076432. [P68254-1]
ENSMUST00000103002; ENSMUSP00000100067; ENSMUSG00000076432. [P68254-1]
GeneIDi22630.
KEGGimmu:102634437.
mmu:22630.
UCSCiuc007ndw.1. mouse. [P68254-1]
uc007ndx.1. mouse. [P68254-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57312 mRNA. Translation: AAC53257.1.
U56243 mRNA. Translation: AAB72023.1.
D87662 mRNA. Translation: BAA13423.1.
AK145568 mRNA. Translation: BAE26517.1.
AK146371 mRNA. Translation: BAE27120.1.
AK150850 mRNA. Translation: BAE29907.1.
AK151437 mRNA. Translation: BAE30400.1.
AK151715 mRNA. Translation: BAE30634.1.
AK151864 mRNA. Translation: BAE30752.1.
AK159403 mRNA. Translation: BAE35055.1.
AK159817 mRNA. Translation: BAE35397.1.
AK163237 mRNA. Translation: BAE37249.1.
AK167397 mRNA. Translation: BAE39486.1.
AL929409 Genomic DNA. Translation: CAI25590.1.
BC080802 mRNA. Translation: AAH80802.1. Different initiation.
BC085299 mRNA. Translation: AAH85299.1. Different initiation.
BC090838 mRNA. Translation: AAH90838.1.
BC106164 mRNA. Translation: AAI06165.1.
CCDSiCCDS25837.1. [P68254-1]
RefSeqiNP_035869.1. NM_011739.3. [P68254-1]
UniGeneiMm.289630.

3D structure databases

SMRiP68254. Positions 1-230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204622. 11 interactions.
IntActiP68254. 13 interactions.
MINTiMINT-1520413.

PTM databases

PhosphoSiteiP68254.

2D gel databases

REPRODUCTION-2DPAGEP68254.

Proteomic databases

MaxQBiP68254.
PaxDbiP68254.
PRIDEiP68254.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049531; ENSMUSP00000106602; ENSMUSG00000076432. [P68254-1]
ENSMUST00000103002; ENSMUSP00000100067; ENSMUSG00000076432. [P68254-1]
GeneIDi22630.
KEGGimmu:102634437.
mmu:22630.
UCSCiuc007ndw.1. mouse. [P68254-1]
uc007ndx.1. mouse. [P68254-2]

Organism-specific databases

CTDi10971.
MGIiMGI:891963. Ywhaq.

Phylogenomic databases

eggNOGiCOG5040.
GeneTreeiENSGT00760000119116.
HOVERGENiHBG050423.
InParanoidiP68254.
KOiK16197.
PhylomeDBiP68254.
TreeFamiTF102002.

Enzyme and pathway databases

ReactomeiREACT_302653. Translocation of GLUT4 to the plasma membrane.
REACT_318722. Activation of BAD and translocation to mitochondria.

Miscellaneous databases

NextBioi302999.
PROiP68254.
SOURCEiSearch...

Gene expression databases

BgeeiP68254.
ExpressionAtlasiP68254. baseline and differential.
GenevestigatoriP68254.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins."
    Sladeczek F., Camonis J.H., Burnol A.-F., Le Bouffant F.
    Mol. Gen. Genet. 254:571-577(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CDK16.
    Tissue: Brain.
  2. "Molecular cloning and tissue-specific expression of the mouse homologue of the rat brain 14-3-3 theta protein: characterization of its cellular and developmental pattern of expression in the male germ line."
    Perego L., Berruti G.
    Mol. Reprod. Dev. 47:370-379(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "14-3-3 family members play an important role in tumorigenic transformation of NIH 3T3 cells and retinoic acid-mediated F9 cell differentiation."
    Takihara Y., Irie K., Nomura M., Motaleb M., Matsumoto K., Shimada K.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: 129/Sv.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Amnion, Blastocyst, Bone marrow and Egg.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain, Mammary tumor and Olfactory epithelium.
  7. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 12-49; 61-68; 75-80; 84-115; 128-167 AND 194-245, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry namei1433T_MOUSE
AccessioniPrimary (citable) accession number: P68254
Secondary accession number(s): P35216
, Q3TW69, Q3UJN5, Q5SP76, Q5U423, Q66JR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2004
Last sequence update: October 24, 2004
Last modified: March 31, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.