P68254 (1433T_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3 protein theta

Alternative name(s):
14-3-3 protein tau

Gene names

Name:

Ywhaq

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 By similarity.
Subunit structure	Homodimer By similarity. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 By similarity. Interacts with GAB2 By similarity. Interacts with CDK16. Interacts with the 'Ser-241' phosphorylated form of PDPK1 By similarity. Ref.1
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the 14-3-3 family.
Sequence caution	The sequence AAH80802.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH85299.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara protein targeting Inferred from direct assay PubMed 12446771. Source: MGI small GTPase mediated signal transduction Inferred from physical interaction Ref.2. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein domain specific binding Inferred from direct assay PubMed 12446771. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Lrrk2	Q5S006	3	EBI-400675,EBI-2693710

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P68254-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P68254-2)

The sequence of this isoform differs from the canonical sequence as follows:
     227-245: LWTSDSAGEECDAAEGAEN → FTCVELETVSVCFSLLS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	14-3-3 protein theta		PRO_0000058637
Sites
	Site	56	1	Interaction with phosphoserine on interacting protein By similarity
	Site	127	1	Interaction with phosphoserine on interacting protein By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	3	1	N6-acetyllysine By similarity
	Modified residue	49	1	N6-acetyllysine By similarity
	Modified residue	68	1	N6-acetyllysine By similarity
	Modified residue	115	1	N6-acetyllysine By similarity
	Modified residue	232	1	Phosphoserine; by CK1 Probable
Natural variations
	Alternative sequence	227 – 245	19	LWTSD…EGAEN → FTCVELETVSVCFSLLS in isoform 2.		VSP_016340
Experimental info
	Sequence conflict	26	1	M → I in BAE35397. Ref.4
	Sequence conflict	227	1	L → S in BAE27120. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: E30471260E8B366E	FASTA	245	27,778
	10 20 30 40 50 60 MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN VVGGRRSAWR 70 80 90 100 110 120 VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL LDKYLIANAT NPESKVFYLK 130 140 150 160 170 180 MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ EAFDISKKEM QPTHPIRLGL ALNFSVFYYE 190 200 210 220 230 240 ILNNPELACT LAKTAFDEAI AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN
	Isoform 2 [UniParc]. Checksum: E03CA8AFF03BA947	FASTA	243	27,701
	10 20 30 40 50 60 MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN VVGGRRSAWR 70 80 90 100 110 120 VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL LDKYLIANAT NPESKVFYLK 130 140 150 160 170 180 MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ EAFDISKKEM QPTHPIRLGL ALNFSVFYYE 190 200 210 220 230 240 ILNNPELACT LAKTAFDEAI AELDTLNEDS YKDSTLIMQL LRDNLTFTCV ELETVSVCFS LLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins." Sladeczek F., Camonis J.H., Burnol A.-F., Le Bouffant F. Mol. Gen. Genet. 254:571-577(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CDK16. Tissue: Brain.
[2]	"Molecular cloning and tissue-specific expression of the mouse homologue of the rat brain 14-3-3 theta protein: characterization of its cellular and developmental pattern of expression in the male germ line." Perego L., Berruti G. Mol. Reprod. Dev. 47:370-379(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"14-3-3 family members play an important role in tumorigenic transformation of NIH 3T3 cells and retinoic acid-mediated F9 cell differentiation." Takihara Y., Irie K., Nomura M., Motaleb M., Matsumoto K., Shimada K. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: 129/Sv.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Amnion, Blastocyst, Bone marrow and Egg.
[5]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6 and FVB/N. Tissue: Brain, Mammary tumor and Olfactory epithelium.
[7]	Lubec G., Kang S.U., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 12-49; 61-68; 75-80; 84-115; 128-167 AND 194-245, MASS SPECTROMETRY. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U57312 mRNA. Translation: AAC53257.1. U56243 mRNA. Translation: AAB72023.1. D87662 mRNA. Translation: BAA13423.1. AK145568 mRNA. Translation: BAE26517.1. AK146371 mRNA. Translation: BAE27120.1. AK150850 mRNA. Translation: BAE29907.1. AK151437 mRNA. Translation: BAE30400.1. AK151715 mRNA. Translation: BAE30634.1. AK151864 mRNA. Translation: BAE30752.1. AK159403 mRNA. Translation: BAE35055.1. AK159817 mRNA. Translation: BAE35397.1. AK163237 mRNA. Translation: BAE37249.1. AK167397 mRNA. Translation: BAE39486.1. AL929409 Genomic DNA. Translation: CAI25590.1. BC080802 mRNA. Translation: AAH80802.1. Different initiation. BC085299 mRNA. Translation: AAH85299.1. Different initiation. BC090838 mRNA. Translation: AAH90838.1. BC106164 mRNA. Translation: AAI06165.1.
IPI	IPI00408378. IPI00656269.
RefSeq	NP_035869.1. NM_011739.3.
UniGene	Mm.289630. Mm.458320.
3D structure databases
ProteinModelPortal	P68254.
SMR	P68254. Positions 1-230.
ModBase	Search...
Protein-protein interaction databases
IntAct	P68254. 7 interactions.
PTM databases
PhosphoSite	P68254.
2D gel databases
REPRODUCTION-2DPAGE	P68254.
Proteomic databases
PaxDb	P68254.
PRIDE	P68254.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000049531; ENSMUSP00000106602; ENSMUSG00000076432. ENSMUST00000103002; ENSMUSP00000100067; ENSMUSG00000076432.
GeneID	22630.
KEGG	mmu:22630.
UCSC	uc007ndx.1. mouse.
Organism-specific databases
CTD	10971.
MGI	MGI:891963. Ywhaq.
Phylogenomic databases
eggNOG	COG5040.
GeneTree	ENSGT00690000101991.
HOVERGEN	HBG050423.
InParanoid	P68254.
KO	K16197.
OrthoDB	EOG480HXJ.
Enzyme and pathway databases
Reactome	REACT_147847. Translocation of Glut4 to the Plasma Membrane.
Gene expression databases
ArrayExpress	P68254.
Bgee	P68254.
Genevestigator	P68254.
Family and domain databases
Gene3D	1.20.190.20. 1 hit.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	YWHAQ. mouse.
NextBio	302999.
SOURCE	Search...

Entry information

Entry name

1433T_MOUSE

Accession

Primary (citable) accession number: P68254
Secondary accession number(s): P35216 , Q3TW69, Q3UJN5, Q5SP76, Q5U423, Q66JR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program