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P68254

- 1433T_MOUSE

UniProt

P68254 - 1433T_MOUSE

Protein

14-3-3 protein theta

Gene

Ywhaq

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei56 – 561Interaction with phosphoserine on interacting proteinBy similarity
    Sitei127 – 1271Interaction with phosphoserine on interacting proteinBy similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein domain specific binding Source: MGI

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: Ensembl
    2. protein targeting Source: MGI
    3. signal transduction Source: MGI
    4. small GTPase mediated signal transduction Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    14-3-3 protein theta
    Alternative name(s):
    14-3-3 protein tau
    Gene namesi
    Name:Ywhaq
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:891963. Ywhaq.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic vesicle membrane Source: Reactome
    2. cytosol Source: Reactome
    3. extracellular vesicular exosome Source: Ensembl
    4. protein complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 24524514-3-3 protein thetaPRO_0000058637Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei3 – 31N6-acetyllysineBy similarity
    Modified residuei49 – 491N6-acetyllysineBy similarity
    Modified residuei68 – 681N6-acetyllysineBy similarity
    Modified residuei115 – 1151N6-acetyllysineBy similarity
    Modified residuei232 – 2321Phosphoserine; by CK1Curated

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP68254.
    PaxDbiP68254.
    PRIDEiP68254.

    2D gel databases

    REPRODUCTION-2DPAGEP68254.

    PTM databases

    PhosphoSiteiP68254.

    Expressioni

    Gene expression databases

    ArrayExpressiP68254.
    BgeeiP68254.
    GenevestigatoriP68254.

    Interactioni

    Subunit structurei

    Homodimer By similarity. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 By similarity. Interacts with GAB2 By similarity. Interacts with CDK16. Interacts with the 'Ser-241' phosphorylated form of PDPK1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Lrrk2Q5S0063EBI-400675,EBI-2693710

    Protein-protein interaction databases

    BioGridi204622. 9 interactions.
    IntActiP68254. 13 interactions.
    MINTiMINT-1520413.

    Structurei

    3D structure databases

    ProteinModelPortaliP68254.
    SMRiP68254. Positions 1-230.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 14-3-3 family.Curated

    Phylogenomic databases

    eggNOGiCOG5040.
    GeneTreeiENSGT00730000110424.
    HOVERGENiHBG050423.
    InParanoidiP68254.
    KOiK16197.
    PhylomeDBiP68254.
    TreeFamiTF102002.

    Family and domain databases

    Gene3Di1.20.190.20. 1 hit.
    InterProiIPR000308. 14-3-3.
    IPR023409. 14-3-3_CS.
    IPR023410. 14-3-3_domain.
    [Graphical view]
    PANTHERiPTHR18860. PTHR18860. 1 hit.
    PfamiPF00244. 14-3-3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000868. 14-3-3. 1 hit.
    PRINTSiPR00305. 1433ZETA.
    SMARTiSM00101. 14_3_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48445. SSF48445. 1 hit.
    PROSITEiPS00796. 1433_1. 1 hit.
    PS00797. 1433_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P68254-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN    50
    VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL 100
    LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ 150
    EAFDISKKEM QPTHPIRLGL ALNFSVFYYE ILNNPELACT LAKTAFDEAI 200
    AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN 245
    Length:245
    Mass (Da):27,778
    Last modified:October 25, 2004 - v1
    Checksum:iE30471260E8B366E
    GO
    Isoform 2 (identifier: P68254-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         227-245: LWTSDSAGEECDAAEGAEN → FTCVELETVSVCFSLLS

    Note: No experimental confirmation available.

    Show »
    Length:243
    Mass (Da):27,701
    Checksum:iE03CA8AFF03BA947
    GO

    Sequence cautioni

    The sequence AAH80802.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH85299.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261M → I in BAE35397. (PubMed:16141072)Curated
    Sequence conflicti227 – 2271L → S in BAE27120. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei227 – 24519LWTSD…EGAEN → FTCVELETVSVCFSLLS in isoform 2. 1 PublicationVSP_016340Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57312 mRNA. Translation: AAC53257.1.
    U56243 mRNA. Translation: AAB72023.1.
    D87662 mRNA. Translation: BAA13423.1.
    AK145568 mRNA. Translation: BAE26517.1.
    AK146371 mRNA. Translation: BAE27120.1.
    AK150850 mRNA. Translation: BAE29907.1.
    AK151437 mRNA. Translation: BAE30400.1.
    AK151715 mRNA. Translation: BAE30634.1.
    AK151864 mRNA. Translation: BAE30752.1.
    AK159403 mRNA. Translation: BAE35055.1.
    AK159817 mRNA. Translation: BAE35397.1.
    AK163237 mRNA. Translation: BAE37249.1.
    AK167397 mRNA. Translation: BAE39486.1.
    AL929409 Genomic DNA. Translation: CAI25590.1.
    BC080802 mRNA. Translation: AAH80802.1. Different initiation.
    BC085299 mRNA. Translation: AAH85299.1. Different initiation.
    BC090838 mRNA. Translation: AAH90838.1.
    BC106164 mRNA. Translation: AAI06165.1.
    CCDSiCCDS25837.1. [P68254-1]
    RefSeqiNP_035869.1. NM_011739.3. [P68254-1]
    XP_006538470.1. XM_006538407.1. [P68254-1]
    XP_006543919.1. XM_006543856.1. [P68254-1]
    UniGeneiMm.289630.

    Genome annotation databases

    EnsembliENSMUST00000049531; ENSMUSP00000106602; ENSMUSG00000076432. [P68254-1]
    ENSMUST00000103002; ENSMUSP00000100067; ENSMUSG00000076432. [P68254-1]
    GeneIDi102634437.
    22630.
    KEGGimmu:102634437.
    mmu:22630.
    UCSCiuc007ndw.1. mouse. [P68254-1]
    uc007ndx.1. mouse. [P68254-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57312 mRNA. Translation: AAC53257.1 .
    U56243 mRNA. Translation: AAB72023.1 .
    D87662 mRNA. Translation: BAA13423.1 .
    AK145568 mRNA. Translation: BAE26517.1 .
    AK146371 mRNA. Translation: BAE27120.1 .
    AK150850 mRNA. Translation: BAE29907.1 .
    AK151437 mRNA. Translation: BAE30400.1 .
    AK151715 mRNA. Translation: BAE30634.1 .
    AK151864 mRNA. Translation: BAE30752.1 .
    AK159403 mRNA. Translation: BAE35055.1 .
    AK159817 mRNA. Translation: BAE35397.1 .
    AK163237 mRNA. Translation: BAE37249.1 .
    AK167397 mRNA. Translation: BAE39486.1 .
    AL929409 Genomic DNA. Translation: CAI25590.1 .
    BC080802 mRNA. Translation: AAH80802.1 . Different initiation.
    BC085299 mRNA. Translation: AAH85299.1 . Different initiation.
    BC090838 mRNA. Translation: AAH90838.1 .
    BC106164 mRNA. Translation: AAI06165.1 .
    CCDSi CCDS25837.1. [P68254-1 ]
    RefSeqi NP_035869.1. NM_011739.3. [P68254-1 ]
    XP_006538470.1. XM_006538407.1. [P68254-1 ]
    XP_006543919.1. XM_006543856.1. [P68254-1 ]
    UniGenei Mm.289630.

    3D structure databases

    ProteinModelPortali P68254.
    SMRi P68254. Positions 1-230.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204622. 9 interactions.
    IntActi P68254. 13 interactions.
    MINTi MINT-1520413.

    PTM databases

    PhosphoSitei P68254.

    2D gel databases

    REPRODUCTION-2DPAGE P68254.

    Proteomic databases

    MaxQBi P68254.
    PaxDbi P68254.
    PRIDEi P68254.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000049531 ; ENSMUSP00000106602 ; ENSMUSG00000076432 . [P68254-1 ]
    ENSMUST00000103002 ; ENSMUSP00000100067 ; ENSMUSG00000076432 . [P68254-1 ]
    GeneIDi 102634437.
    22630.
    KEGGi mmu:102634437.
    mmu:22630.
    UCSCi uc007ndw.1. mouse. [P68254-1 ]
    uc007ndx.1. mouse. [P68254-2 ]

    Organism-specific databases

    CTDi 10971.
    MGIi MGI:891963. Ywhaq.

    Phylogenomic databases

    eggNOGi COG5040.
    GeneTreei ENSGT00730000110424.
    HOVERGENi HBG050423.
    InParanoidi P68254.
    KOi K16197.
    PhylomeDBi P68254.
    TreeFami TF102002.

    Enzyme and pathway databases

    Reactomei REACT_199054. Translocation of GLUT4 to the plasma membrane.

    Miscellaneous databases

    ChiTaRSi YWHAQ. mouse.
    NextBioi 302999.
    PROi P68254.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P68254.
    Bgeei P68254.
    Genevestigatori P68254.

    Family and domain databases

    Gene3Di 1.20.190.20. 1 hit.
    InterProi IPR000308. 14-3-3.
    IPR023409. 14-3-3_CS.
    IPR023410. 14-3-3_domain.
    [Graphical view ]
    PANTHERi PTHR18860. PTHR18860. 1 hit.
    Pfami PF00244. 14-3-3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000868. 14-3-3. 1 hit.
    PRINTSi PR00305. 1433ZETA.
    SMARTi SM00101. 14_3_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48445. SSF48445. 1 hit.
    PROSITEi PS00796. 1433_1. 1 hit.
    PS00797. 1433_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins."
      Sladeczek F., Camonis J.H., Burnol A.-F., Le Bouffant F.
      Mol. Gen. Genet. 254:571-577(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CDK16.
      Tissue: Brain.
    2. "Molecular cloning and tissue-specific expression of the mouse homologue of the rat brain 14-3-3 theta protein: characterization of its cellular and developmental pattern of expression in the male germ line."
      Perego L., Berruti G.
      Mol. Reprod. Dev. 47:370-379(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "14-3-3 family members play an important role in tumorigenic transformation of NIH 3T3 cells and retinoic acid-mediated F9 cell differentiation."
      Takihara Y., Irie K., Nomura M., Motaleb M., Matsumoto K., Shimada K.
      Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: 129/Sv.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Amnion, Blastocyst, Bone marrow and Egg.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain, Mammary tumor and Olfactory epithelium.
    7. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 12-49; 61-68; 75-80; 84-115; 128-167 AND 194-245, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.

    Entry informationi

    Entry namei1433T_MOUSE
    AccessioniPrimary (citable) accession number: P68254
    Secondary accession number(s): P35216
    , Q3TW69, Q3UJN5, Q5SP76, Q5U423, Q66JR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2004
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3