Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

14-3-3 protein theta

Gene

Ywhaq

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity).By similarity

GO - Molecular functioni

  • protein domain specific binding Source: MGI
  • protein N-terminus binding Source: MGI

GO - Biological processi

  • negative regulation of ion transmembrane transport Source: Ensembl
  • negative regulation of transcription, DNA-templated Source: MGI
  • protein targeting Source: MGI
  • signal transduction Source: MGI
  • small GTPase mediated signal transduction Source: MGI
  • substantia nigra development Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein theta
Alternative name(s):
14-3-3 protein tau
Gene namesi
Name:Ywhaq
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:891963. Ywhaq.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586371 – 24514-3-3 protein thetaAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei3N6-acetyllysineBy similarity1
Modified residuei49N6-acetyllysineBy similarity1
Modified residuei68N6-acetyllysineBy similarity1
Modified residuei92PhosphoserineCombined sources1
Modified residuei115N6-acetyllysineBy similarity1
Modified residuei232Phosphoserine; by CK1Curated1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP68254.
PaxDbiP68254.
PeptideAtlasiP68254.
PRIDEiP68254.

2D gel databases

REPRODUCTION-2DPAGEP68254.

PTM databases

iPTMnetiP68254.
PhosphoSitePlusiP68254.
SwissPalmiP68254.

Expressioni

Gene expression databases

BgeeiENSMUSG00000076432.
ExpressionAtlasiP68254. baseline and differential.
GenevisibleiP68254. MM.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 (By similarity). Interacts with GAB2. Interacts with RGS7 (phosphorylated form) (By similarity). Interacts with CDK16 (PubMed:9197417). Interacts with the 'Ser-241' phosphorylated form of PDPK1 (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Interaction with phosphoserine on interacting proteinBy similarity1
Sitei127Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
Gja1P232423EBI-400675,EBI-298630
Itga5P116882EBI-400675,EBI-6477055
Lrrk2Q5S0063EBI-400675,EBI-2693710

GO - Molecular functioni

  • protein domain specific binding Source: MGI
  • protein N-terminus binding Source: MGI

Protein-protein interaction databases

BioGridi204622. 11 interactors.
IntActiP68254. 16 interactors.
MINTiMINT-1520413.
STRINGi10090.ENSMUSP00000100067.

Structurei

3D structure databases

ProteinModelPortaliP68254.
SMRiP68254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOVERGENiHBG050423.
InParanoidiP68254.
KOiK16197.
PhylomeDBiP68254.
TreeFamiTF102002.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P68254-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN
60 70 80 90 100
VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL
110 120 130 140 150
LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ
160 170 180 190 200
EAFDISKKEM QPTHPIRLGL ALNFSVFYYE ILNNPELACT LAKTAFDEAI
210 220 230 240
AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN
Length:245
Mass (Da):27,778
Last modified:October 25, 2004 - v1
Checksum:iE30471260E8B366E
GO
Isoform 2 (identifier: P68254-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     227-245: LWTSDSAGEECDAAEGAEN → FTCVELETVSVCFSLLS

Note: No experimental confirmation available.
Show »
Length:243
Mass (Da):27,701
Checksum:iE03CA8AFF03BA947
GO

Sequence cautioni

The sequence AAH80802 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH85299 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26M → I in BAE35397 (PubMed:16141072).Curated1
Sequence conflicti227L → S in BAE27120 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016340227 – 245LWTSD…EGAEN → FTCVELETVSVCFSLLS in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57312 mRNA. Translation: AAC53257.1.
U56243 mRNA. Translation: AAB72023.1.
D87662 mRNA. Translation: BAA13423.1.
AK145568 mRNA. Translation: BAE26517.1.
AK146371 mRNA. Translation: BAE27120.1.
AK150850 mRNA. Translation: BAE29907.1.
AK151437 mRNA. Translation: BAE30400.1.
AK151715 mRNA. Translation: BAE30634.1.
AK151864 mRNA. Translation: BAE30752.1.
AK159403 mRNA. Translation: BAE35055.1.
AK159817 mRNA. Translation: BAE35397.1.
AK163237 mRNA. Translation: BAE37249.1.
AK167397 mRNA. Translation: BAE39486.1.
AL929409 Genomic DNA. Translation: CAI25590.1.
BC080802 mRNA. Translation: AAH80802.1. Different initiation.
BC085299 mRNA. Translation: AAH85299.1. Different initiation.
BC090838 mRNA. Translation: AAH90838.1.
BC106164 mRNA. Translation: AAI06165.1.
CCDSiCCDS25837.1. [P68254-1]
RefSeqiNP_035869.1. NM_011739.3. [P68254-1]
UniGeneiMm.289630.

Genome annotation databases

EnsembliENSMUST00000049531; ENSMUSP00000106602; ENSMUSG00000076432. [P68254-1]
ENSMUST00000103002; ENSMUSP00000100067; ENSMUSG00000076432. [P68254-1]
GeneIDi22630.
KEGGimmu:22630.
UCSCiuc007ndw.1. mouse. [P68254-1]
uc007ndx.1. mouse. [P68254-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57312 mRNA. Translation: AAC53257.1.
U56243 mRNA. Translation: AAB72023.1.
D87662 mRNA. Translation: BAA13423.1.
AK145568 mRNA. Translation: BAE26517.1.
AK146371 mRNA. Translation: BAE27120.1.
AK150850 mRNA. Translation: BAE29907.1.
AK151437 mRNA. Translation: BAE30400.1.
AK151715 mRNA. Translation: BAE30634.1.
AK151864 mRNA. Translation: BAE30752.1.
AK159403 mRNA. Translation: BAE35055.1.
AK159817 mRNA. Translation: BAE35397.1.
AK163237 mRNA. Translation: BAE37249.1.
AK167397 mRNA. Translation: BAE39486.1.
AL929409 Genomic DNA. Translation: CAI25590.1.
BC080802 mRNA. Translation: AAH80802.1. Different initiation.
BC085299 mRNA. Translation: AAH85299.1. Different initiation.
BC090838 mRNA. Translation: AAH90838.1.
BC106164 mRNA. Translation: AAI06165.1.
CCDSiCCDS25837.1. [P68254-1]
RefSeqiNP_035869.1. NM_011739.3. [P68254-1]
UniGeneiMm.289630.

3D structure databases

ProteinModelPortaliP68254.
SMRiP68254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204622. 11 interactors.
IntActiP68254. 16 interactors.
MINTiMINT-1520413.
STRINGi10090.ENSMUSP00000100067.

PTM databases

iPTMnetiP68254.
PhosphoSitePlusiP68254.
SwissPalmiP68254.

2D gel databases

REPRODUCTION-2DPAGEP68254.

Proteomic databases

MaxQBiP68254.
PaxDbiP68254.
PeptideAtlasiP68254.
PRIDEiP68254.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049531; ENSMUSP00000106602; ENSMUSG00000076432. [P68254-1]
ENSMUST00000103002; ENSMUSP00000100067; ENSMUSG00000076432. [P68254-1]
GeneIDi22630.
KEGGimmu:22630.
UCSCiuc007ndw.1. mouse. [P68254-1]
uc007ndx.1. mouse. [P68254-2]

Organism-specific databases

CTDi10971.
55755.
MGIiMGI:891963. Ywhaq.

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOVERGENiHBG050423.
InParanoidiP68254.
KOiK16197.
PhylomeDBiP68254.
TreeFamiTF102002.

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Miscellaneous databases

PROiP68254.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000076432.
ExpressionAtlasiP68254. baseline and differential.
GenevisibleiP68254. MM.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei1433T_MOUSE
AccessioniPrimary (citable) accession number: P68254
Secondary accession number(s): P35216
, Q3TW69, Q3UJN5, Q5SP76, Q5U423, Q66JR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.