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P68254

- 1433T_MOUSE

UniProt

P68254 - 1433T_MOUSE

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Protein

14-3-3 protein theta

Gene
Ywhaq
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei56 – 561Interaction with phosphoserine on interacting protein By similarity
Sitei127 – 1271Interaction with phosphoserine on interacting protein By similarity

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein domain specific binding Source: MGI

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: Ensembl
  2. protein targeting Source: MGI
  3. signal transduction Source: MGI
  4. small GTPase mediated signal transduction Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein theta
Alternative name(s):
14-3-3 protein tau
Gene namesi
Name:Ywhaq
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:891963. Ywhaq.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic vesicle membrane Source: Reactome
  2. cytosol Source: Reactome
  3. extracellular vesicular exosome Source: Ensembl
  4. protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24524514-3-3 protein thetaPRO_0000058637Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei3 – 31N6-acetyllysine By similarity
Modified residuei49 – 491N6-acetyllysine By similarity
Modified residuei68 – 681N6-acetyllysine By similarity
Modified residuei115 – 1151N6-acetyllysine By similarity
Modified residuei232 – 2321Phosphoserine; by CK1 Inferred

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP68254.
PaxDbiP68254.
PRIDEiP68254.

2D gel databases

REPRODUCTION-2DPAGEP68254.

PTM databases

PhosphoSiteiP68254.

Expressioni

Gene expression databases

ArrayExpressiP68254.
BgeeiP68254.
GenevestigatoriP68254.

Interactioni

Subunit structurei

Homodimer By similarity. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 By similarity. Interacts with GAB2 By similarity. Interacts with CDK16. Interacts with the 'Ser-241' phosphorylated form of PDPK1 By similarity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Lrrk2Q5S0063EBI-400675,EBI-2693710

Protein-protein interaction databases

BioGridi204622. 9 interactions.
IntActiP68254. 13 interactions.
MINTiMINT-1520413.

Structurei

3D structure databases

ProteinModelPortaliP68254.
SMRiP68254. Positions 1-230.

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.

Phylogenomic databases

eggNOGiCOG5040.
GeneTreeiENSGT00730000110424.
HOVERGENiHBG050423.
InParanoidiP68254.
KOiK16197.
PhylomeDBiP68254.
TreeFamiTF102002.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P68254-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN    50
VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL 100
LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ 150
EAFDISKKEM QPTHPIRLGL ALNFSVFYYE ILNNPELACT LAKTAFDEAI 200
AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN 245
Length:245
Mass (Da):27,778
Last modified:October 25, 2004 - v1
Checksum:iE30471260E8B366E
GO
Isoform 2 (identifier: P68254-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     227-245: LWTSDSAGEECDAAEGAEN → FTCVELETVSVCFSLLS

Note: No experimental confirmation available.

Show »
Length:243
Mass (Da):27,701
Checksum:iE03CA8AFF03BA947
GO

Sequence cautioni

The sequence AAH80802.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH85299.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei227 – 24519LWTSD…EGAEN → FTCVELETVSVCFSLLS in isoform 2. VSP_016340Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261M → I in BAE35397. 1 Publication
Sequence conflicti227 – 2271L → S in BAE27120. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U57312 mRNA. Translation: AAC53257.1.
U56243 mRNA. Translation: AAB72023.1.
D87662 mRNA. Translation: BAA13423.1.
AK145568 mRNA. Translation: BAE26517.1.
AK146371 mRNA. Translation: BAE27120.1.
AK150850 mRNA. Translation: BAE29907.1.
AK151437 mRNA. Translation: BAE30400.1.
AK151715 mRNA. Translation: BAE30634.1.
AK151864 mRNA. Translation: BAE30752.1.
AK159403 mRNA. Translation: BAE35055.1.
AK159817 mRNA. Translation: BAE35397.1.
AK163237 mRNA. Translation: BAE37249.1.
AK167397 mRNA. Translation: BAE39486.1.
AL929409 Genomic DNA. Translation: CAI25590.1.
BC080802 mRNA. Translation: AAH80802.1. Different initiation.
BC085299 mRNA. Translation: AAH85299.1. Different initiation.
BC090838 mRNA. Translation: AAH90838.1.
BC106164 mRNA. Translation: AAI06165.1.
CCDSiCCDS25837.1. [P68254-1]
RefSeqiNP_035869.1. NM_011739.3. [P68254-1]
XP_006538470.1. XM_006538407.1. [P68254-1]
XP_006543919.1. XM_006543856.1. [P68254-1]
UniGeneiMm.289630.

Genome annotation databases

EnsembliENSMUST00000049531; ENSMUSP00000106602; ENSMUSG00000076432. [P68254-1]
ENSMUST00000103002; ENSMUSP00000100067; ENSMUSG00000076432. [P68254-1]
GeneIDi102634437.
22630.
KEGGimmu:102634437.
mmu:22630.
UCSCiuc007ndw.1. mouse. [P68254-1]
uc007ndx.1. mouse. [P68254-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U57312 mRNA. Translation: AAC53257.1 .
U56243 mRNA. Translation: AAB72023.1 .
D87662 mRNA. Translation: BAA13423.1 .
AK145568 mRNA. Translation: BAE26517.1 .
AK146371 mRNA. Translation: BAE27120.1 .
AK150850 mRNA. Translation: BAE29907.1 .
AK151437 mRNA. Translation: BAE30400.1 .
AK151715 mRNA. Translation: BAE30634.1 .
AK151864 mRNA. Translation: BAE30752.1 .
AK159403 mRNA. Translation: BAE35055.1 .
AK159817 mRNA. Translation: BAE35397.1 .
AK163237 mRNA. Translation: BAE37249.1 .
AK167397 mRNA. Translation: BAE39486.1 .
AL929409 Genomic DNA. Translation: CAI25590.1 .
BC080802 mRNA. Translation: AAH80802.1 . Different initiation.
BC085299 mRNA. Translation: AAH85299.1 . Different initiation.
BC090838 mRNA. Translation: AAH90838.1 .
BC106164 mRNA. Translation: AAI06165.1 .
CCDSi CCDS25837.1. [P68254-1 ]
RefSeqi NP_035869.1. NM_011739.3. [P68254-1 ]
XP_006538470.1. XM_006538407.1. [P68254-1 ]
XP_006543919.1. XM_006543856.1. [P68254-1 ]
UniGenei Mm.289630.

3D structure databases

ProteinModelPortali P68254.
SMRi P68254. Positions 1-230.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204622. 9 interactions.
IntActi P68254. 13 interactions.
MINTi MINT-1520413.

PTM databases

PhosphoSitei P68254.

2D gel databases

REPRODUCTION-2DPAGE P68254.

Proteomic databases

MaxQBi P68254.
PaxDbi P68254.
PRIDEi P68254.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000049531 ; ENSMUSP00000106602 ; ENSMUSG00000076432 . [P68254-1 ]
ENSMUST00000103002 ; ENSMUSP00000100067 ; ENSMUSG00000076432 . [P68254-1 ]
GeneIDi 102634437.
22630.
KEGGi mmu:102634437.
mmu:22630.
UCSCi uc007ndw.1. mouse. [P68254-1 ]
uc007ndx.1. mouse. [P68254-2 ]

Organism-specific databases

CTDi 10971.
MGIi MGI:891963. Ywhaq.

Phylogenomic databases

eggNOGi COG5040.
GeneTreei ENSGT00730000110424.
HOVERGENi HBG050423.
InParanoidi P68254.
KOi K16197.
PhylomeDBi P68254.
TreeFami TF102002.

Enzyme and pathway databases

Reactomei REACT_199054. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

ChiTaRSi YWHAQ. mouse.
NextBioi 302999.
PROi P68254.
SOURCEi Search...

Gene expression databases

ArrayExpressi P68254.
Bgeei P68254.
Genevestigatori P68254.

Family and domain databases

Gene3Di 1.20.190.20. 1 hit.
InterProi IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view ]
PANTHERi PTHR18860. PTHR18860. 1 hit.
Pfami PF00244. 14-3-3. 1 hit.
[Graphical view ]
PIRSFi PIRSF000868. 14-3-3. 1 hit.
PRINTSi PR00305. 1433ZETA.
SMARTi SM00101. 14_3_3. 1 hit.
[Graphical view ]
SUPFAMi SSF48445. SSF48445. 1 hit.
PROSITEi PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins."
    Sladeczek F., Camonis J.H., Burnol A.-F., Le Bouffant F.
    Mol. Gen. Genet. 254:571-577(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CDK16.
    Tissue: Brain.
  2. "Molecular cloning and tissue-specific expression of the mouse homologue of the rat brain 14-3-3 theta protein: characterization of its cellular and developmental pattern of expression in the male germ line."
    Perego L., Berruti G.
    Mol. Reprod. Dev. 47:370-379(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "14-3-3 family members play an important role in tumorigenic transformation of NIH 3T3 cells and retinoic acid-mediated F9 cell differentiation."
    Takihara Y., Irie K., Nomura M., Motaleb M., Matsumoto K., Shimada K.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: 129/Sv.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Amnion, Blastocyst, Bone marrow and Egg.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain, Mammary tumor and Olfactory epithelium.
  7. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 12-49; 61-68; 75-80; 84-115; 128-167 AND 194-245, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry namei1433T_MOUSE
AccessioniPrimary (citable) accession number: P68254
Secondary accession number(s): P35216
, Q3TW69, Q3UJN5, Q5SP76, Q5U423, Q66JR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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