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P68254 (1433T_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
14-3-3 protein theta
Alternative name(s):
14-3-3 protein tau
Gene names
Name:Ywhaq
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 By similarity.

Subunit structure

Homodimer By similarity. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 By similarity. Interacts with GAB2 By similarity. Interacts with CDK16. Interacts with the 'Ser-241' phosphorylated form of PDPK1 By similarity. Ref.1

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the 14-3-3 family.

Sequence caution

The sequence AAH80802.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH85299.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Lrrk2Q5S0063EBI-400675,EBI-2693710

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P68254-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P68254-2)

The sequence of this isoform differs from the canonical sequence as follows:
     227-245: LWTSDSAGEECDAAEGAEN → FTCVELETVSVCFSLLS
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 24524514-3-3 protein theta
PRO_0000058637

Sites

Site561Interaction with phosphoserine on interacting protein By similarity
Site1271Interaction with phosphoserine on interacting protein By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue31N6-acetyllysine By similarity
Modified residue491N6-acetyllysine By similarity
Modified residue681N6-acetyllysine By similarity
Modified residue1151N6-acetyllysine By similarity
Modified residue2321Phosphoserine; by CK1 Probable

Natural variations

Alternative sequence227 – 24519LWTSD…EGAEN → FTCVELETVSVCFSLLS in isoform 2.
VSP_016340

Experimental info

Sequence conflict261M → I in BAE35397. Ref.4
Sequence conflict2271L → S in BAE27120. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: E30471260E8B366E

FASTA24527,778
        10         20         30         40         50         60 
MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN VVGGRRSAWR 

        70         80         90        100        110        120 
VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL LDKYLIANAT NPESKVFYLK 

       130        140        150        160        170        180 
MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ EAFDISKKEM QPTHPIRLGL ALNFSVFYYE 

       190        200        210        220        230        240 
ILNNPELACT LAKTAFDEAI AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA 


EGAEN 

« Hide

Isoform 2 [UniParc].

Checksum: E03CA8AFF03BA947
Show »

FASTA24327,701

References

« Hide 'large scale' references
[1]"The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins."
Sladeczek F., Camonis J.H., Burnol A.-F., Le Bouffant F.
Mol. Gen. Genet. 254:571-577(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CDK16.
Tissue: Brain.
[2]"Molecular cloning and tissue-specific expression of the mouse homologue of the rat brain 14-3-3 theta protein: characterization of its cellular and developmental pattern of expression in the male germ line."
Perego L., Berruti G.
Mol. Reprod. Dev. 47:370-379(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"14-3-3 family members play an important role in tumorigenic transformation of NIH 3T3 cells and retinoic acid-mediated F9 cell differentiation."
Takihara Y., Irie K., Nomura M., Motaleb M., Matsumoto K., Shimada K.
Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: 129/Sv.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Amnion, Blastocyst, Bone marrow and Egg.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6 and FVB/N.
Tissue: Brain, Mammary tumor and Olfactory epithelium.
[7]Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 12-49; 61-68; 75-80; 84-115; 128-167 AND 194-245, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6 and OF1.
Tissue: Brain and Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U57312 mRNA. Translation: AAC53257.1.
U56243 mRNA. Translation: AAB72023.1.
D87662 mRNA. Translation: BAA13423.1.
AK145568 mRNA. Translation: BAE26517.1.
AK146371 mRNA. Translation: BAE27120.1.
AK150850 mRNA. Translation: BAE29907.1.
AK151437 mRNA. Translation: BAE30400.1.
AK151715 mRNA. Translation: BAE30634.1.
AK151864 mRNA. Translation: BAE30752.1.
AK159403 mRNA. Translation: BAE35055.1.
AK159817 mRNA. Translation: BAE35397.1.
AK163237 mRNA. Translation: BAE37249.1.
AK167397 mRNA. Translation: BAE39486.1.
AL929409 Genomic DNA. Translation: CAI25590.1.
BC080802 mRNA. Translation: AAH80802.1. Different initiation.
BC085299 mRNA. Translation: AAH85299.1. Different initiation.
BC090838 mRNA. Translation: AAH90838.1.
BC106164 mRNA. Translation: AAI06165.1.
CCDSCCDS25837.1. [P68254-1]
RefSeqNP_035869.1. NM_011739.3. [P68254-1]
XP_006538470.1. XM_006538407.1. [P68254-1]
XP_006543919.1. XM_006543856.1. [P68254-1]
UniGeneMm.289630.

3D structure databases

ProteinModelPortalP68254.
SMRP68254. Positions 1-230.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204622. 9 interactions.
IntActP68254. 13 interactions.
MINTMINT-1520413.

PTM databases

PhosphoSiteP68254.

2D gel databases

REPRODUCTION-2DPAGEP68254.

Proteomic databases

MaxQBP68254.
PaxDbP68254.
PRIDEP68254.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000049531; ENSMUSP00000106602; ENSMUSG00000076432. [P68254-1]
ENSMUST00000103002; ENSMUSP00000100067; ENSMUSG00000076432. [P68254-1]
GeneID102634437.
22630.
KEGGmmu:102634437.
mmu:22630.
UCSCuc007ndw.1. mouse. [P68254-1]
uc007ndx.1. mouse. [P68254-2]

Organism-specific databases

CTD10971.
MGIMGI:891963. Ywhaq.

Phylogenomic databases

eggNOGCOG5040.
GeneTreeENSGT00730000110424.
HOVERGENHBG050423.
InParanoidP68254.
KOK16197.
PhylomeDBP68254.
TreeFamTF102002.

Enzyme and pathway databases

ReactomeREACT_147847. Translocation of Glut4 to the Plasma Membrane.

Gene expression databases

ArrayExpressP68254.
BgeeP68254.
GenevestigatorP68254.

Family and domain databases

Gene3D1.20.190.20. 1 hit.
InterProIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERPTHR18860. PTHR18860. 1 hit.
PfamPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFPIRSF000868. 14-3-3. 1 hit.
PRINTSPR00305. 1433ZETA.
SMARTSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMSSF48445. SSF48445. 1 hit.
PROSITEPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSYWHAQ. mouse.
NextBio302999.
PROP68254.
SOURCESearch...

Entry information

Entry name1433T_MOUSE
AccessionPrimary (citable) accession number: P68254
Secondary accession number(s): P35216 expand/collapse secondary AC list , Q3TW69, Q3UJN5, Q5SP76, Q5U423, Q66JR6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot