P68253 (1433G_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3 protein gamma

Alternative name(s):
Protein kinase C inhibitor protein 1
Short name=KCIP-1

Gene names

Name:

YWHAG

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	158 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Subunit structure	Homodimer. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2 and SAMSN1 By similarity. Interacts with PKA-phosphorylated AANAT. Ref.2
Subcellular location	Cytoplasm.
Post-translational modification	N-terminus residue is acetylated By similarity.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	regulation of neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB regulation of synaptic plasticity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	insulin-like growth factor receptor binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›158	›158	14-3-3 protein gamma		PRO_0000058610
Sites
	Site	34	1	Interaction with phosphoserine on interacting protein By similarity
	Site	59	1	Interaction with phosphoserine on interacting protein By similarity
Amino acid modifications
	Modified residue	72	1	Phosphothreonine By similarity
Experimental info
	Non-adjacent residues	48 – 49	2
	Non-adjacent residues	113 – 114	2
	Non-terminal residue	1	1
	Non-terminal residue	158	1

Sequences

	Sequence		Length	Mass (Da)
	P68253 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 69EC73EBA23B7B6F	FASTA	158	18,000
	10 20 30 40 50 60 AAAMKNVTEL NEPLSNEERN LLSVAYKNVV GARRSSWRVI SSIEQKTSFY LKMKGDYYRY 70 80 90 100 110 120 LAEVATGEKR ATVVESSEKA YSEAHEISKE HMQPTHPIRL GLALNYSVFY YEITAFDDAI 130 140 150 AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DQQDDDGG

References

[1]

"Multiple isoforms of a protein kinase C inhibitor (KCIP-1/14-3-3) from sheep brain. Amino acid sequence of phosphorylated forms." Toker A., Sellers L.A., Amess B., Patel Y., Harris A., Aitken A. Eur. J. Biochem. 206:453-461(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Brain.

[2]

"Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis." Ganguly S., Gastel J.A., Weller J.L., Schwartz C., Jaffe H., Namboodiri M.A., Coon S.L., Hickman A.B., Rollag M., Obsil T., Beauverger P., Ferry G., Boutin J.A., Klein D.C. Proc. Natl. Acad. Sci. U.S.A. 98:8083-8088(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AANAT.

Cross-references

Sequence databases
PIR	S78574.
3D structure databases
ProteinModelPortal	P68253.
SMR	P68253. Positions 1-149.
ModBase	Search...
Proteomic databases
PRIDE	P68253.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050423.
Family and domain databases
Gene3D	1.20.190.20. 2 hits.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 2 hits. [Graphical view]
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

1433G_SHEEP

Accession

Primary (citable) accession number: P68253
Secondary accession number(s): P29359

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program