P68252 (1433G_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3 protein gamma

Alternative name(s):
Protein kinase C inhibitor protein 1
Short name=KCIP-1

Cleaved into the following chain:

1. 14-3-3 protein gamma, N-terminally processed

Gene names

Name:

YWHAG

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Ref.3
Subunit structure	Homodimer. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2 and SAMSN1 By similarity. Interacts with PKA-phosphorylated AANAT By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB regulation of synaptic plasticity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	insulin-like growth factor receptor binding Inferred from sequence or structural similarity. Source: UniProtKB protein domain specific binding Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	14-3-3 protein gamma		PRO_0000367906
	Initiator methionine	1	1	Removed; alternate By similarity
	Chain	2 – 247	246	14-3-3 protein gamma, N-terminally processed		PRO_0000058605
Sites
	Site	57	1	Interaction with phosphoserine on interacting protein By similarity
	Site	132	1	Interaction with phosphoserine on interacting protein By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	2	1	N-acetylvaline; in 14-3-3 protein gamma, N-terminally processed
	Modified residue	145	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68252 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: A481633DFAF4455D	FASTA	247	28,253
	10 20 30 40 50 60 MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW 70 80 90 100 110 120 RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQIESK 130 140 150 160 170 180 VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS 190 200 210 220 230 240 VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN

References

[1]	"Expression of 14-3-3 proteins in bovine retinal photoreceptors." Jones J.M., Niikura T., Pinke R.M., Guo W., Molday L., Leykam J., McConnell D.G. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Distinct forms of the protein kinase-dependent activator of tyrosine and tryptophan hydroxylases." Isobe T., Ichimura T., Sunaya T., Okuyama T., Takahashi N., Kuwano R., Takahashi Y. J. Mol. Biol. 217:125-132(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-246.
[3]	"Activation of protein kinase C by purified bovine brain 14-3-3: comparison with tyrosine hydroxylase activation." Tanji M., Horwitz R., Rosenfeld G., Waymire J.C. J. Neurochem. 63:1908-1916(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF043737 mRNA. Translation: AAC02091.1.
IPI	IPI00693106.
PIR	S13610.
UniGene	Bt.107001.
3D structure databases
ProteinModelPortal	P68252.
SMR	P68252. Positions 2-234.
ModBase	Search...
Proteomic databases
PaxDb	P68252.
PRIDE	P68252.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG5040.
HOGENOM	HOG000240379.
HOVERGEN	HBG050423.
InParanoid	P68252.
OrthoDB	EOG48PMM0.
Enzyme and pathway databases
BioCyc	CATTLE:286862-MONOMER.
Family and domain databases
Gene3D	1.20.190.20. 1 hit.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

1433G_BOVIN

Accession

Primary (citable) accession number: P68252
Secondary accession number(s): P29359

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program