P68251 (1433B_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3 protein beta/alpha

Alternative name(s):
Protein kinase C inhibitor protein 1
Short name=KCIP-1

Cleaved into the following chain:

1. 14-3-3 protein beta/alpha, N-terminally processed

Gene names

Name:

YWHAB

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	193 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2 By similarity.
Subunit structure	Homodimer. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1 (phosphorylated form); the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2, YAP1 and KRPCE (phosphorylated form). Interacts with SAMSN1 By similarity. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2 By similarity. Ref.5
Subcellular location	Cytoplasm. Melanosome By similarity.
Post-translational modification	Form alpha differs from form beta in being phosphorylated. Ref.3
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative initiation
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein domain specific binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Note: It is uncertain whether isoform Short is produced by alternative initiation or another biological event.

Isoform Long (identifier: P68251-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P68251-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Note: Contains N-acetylmethionine at position 1.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	14-3-3 protein beta/alpha		PRO_0000367905
	Initiator methionine	1	1	Removed; alternate By similarity
	Chain	2 – 193	192	14-3-3 protein beta/alpha, N-terminally processed		PRO_0000000009
Sites
	Site	58	1	Interaction with phosphoserine on interacting protein By similarity
	Site	92	1	Interaction with phosphoserine on interacting protein By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine; in 14-3-3 protein beta/alpha; alternate By similarity
	Modified residue	2	1	N-acetylthreonine; in 14-3-3 protein beta/alpha, N-terminally processed
	Modified residue	6	1	Phosphoserine By similarity
	Modified residue	70	1	N6-acetyllysine By similarity
	Modified residue	149	1	Phosphoserine Ref.3
Natural variations
	Alternative sequence	1 – 2	2	Missing in isoform Short.		VSP_018636
Experimental info
	Non-adjacent residues	84 – 85	2
	Non-adjacent residues	150 – 151	2
	Non-terminal residue	193	1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 923914FE5226E608	FASTA	193	22,054
	10 20 30 40 50 60 MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS 70 80 90 100 110 120 WRVISSIEQK TERNEKKQQM GKEYKMKGDY FRYLSEVASG DNKQTTVSNS QQAYQEAFEI 130 140 150 160 170 180 SKKEMQPTHP IRLGLALNFS VFYYEILNSP EAIAELDTLN EESYKDSTLI MQLLRDNLTL 190 WTSENQGDEG DAG
	Isoform Short [UniParc]. Checksum: F767595DE749678B	FASTA	191	21,821
	10 20 30 40 50 60 MDKSELVQKA KLAEQAERYD DMAAAMKAVT EQGHELSNEE RNLLSVAYKN VVGARRSSWR 70 80 90 100 110 120 VISSIEQKTE RNEKKQQMGK EYKMKGDYFR YLSEVASGDN KQTTVSNSQQ AYQEAFEISK 130 140 150 160 170 180 KEMQPTHPIR LGLALNFSVF YYEILNSPEA IAELDTLNEE SYKDSTLIMQ LLRDNLTLWT 190 SENQGDEGDA G

References

[1]	"Multiple isoforms of a protein kinase C inhibitor (KCIP-1/14-3-3) from sheep brain. Amino acid sequence of phosphorylated forms." Toker A., Sellers L.A., Amess B., Patel Y., Harris A., Aitken A. Eur. J. Biochem. 206:453-461(1992) [PubMed: 1317796] [Abstract] Cited for: PROTEIN SEQUENCE OF 3-193. Tissue: Brain.
[2]	"Protein kinase C inhibitor proteins. Purification from sheep brain and sequence similarity to lipocortins and 14-3-3 protein." Toker A., Ellis C.A., Sellers L.A., Aitken A. Eur. J. Biochem. 191:421-429(1990) [PubMed: 2143472] [Abstract] Cited for: PROTEIN SEQUENCE OF 3-24 (ISOFORM LONG). Tissue: Brain.
[3]	"14-3-3 alpha and delta are the phosphorylated forms of raf-activating 14-3-3 beta and zeta. In vivo stoichiometric phosphorylation in brain at a Ser-Pro-Glu-Lys motif." Aitken A., Howell S., Jones D., Madrazo J., Patel Y. J. Biol. Chem. 270:5706-5709(1995) [PubMed: 7890696] [Abstract] Cited for: PHOSPHORYLATION AT SER-149, MASS SPECTROMETRY.
[4]	"Electrospray mass spectroscopy analysis with online trapping of posttranslationally modified mammalian and avian brain 14-3-3 isoforms." Aitken A., Patel Y., Martin H., Jones D., Robinson K., Madrazo J., Howell S. J. Protein Chem. 13:463-465(1994) Cited for: ACETYLATION OF N-TERMINUS, MASS SPECTROMETRY.
[5]	"Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis." Ganguly S., Gastel J.A., Weller J.L., Schwartz C., Jaffe H., Namboodiri M.A., Coon S.L., Hickman A.B., Rollag M., Obsil T., Beauverger P., Ferry G., Boutin J.A., Klein D.C. Proc. Natl. Acad. Sci. U.S.A. 98:8083-8088(2001) [PubMed: 11427721] [Abstract] Cited for: INTERACTION WITH AANAT.

Cross-references

3D structure databases
ProteinModelPortal	P68251.
SMR	P68251. Positions 3-184.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050423.
Family and domain databases
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
Gene3D	G3DSA:1.20.190.20. 14-3-3. 2 hits.
PANTHER	PTHR18860. 14-3-3. 1 hit.
Pfam	PF00244. 14-3-3. 2 hits. [Graphical view]
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

1433B_SHEEP

Accession

Primary (citable) accession number: P68251
Secondary accession number(s): P29358

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program