14-3-3 protein beta/alpha - Bos taurus (Bovine)

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Cleaved into the following chain:

Molecule processing

Sites

Amino acid modifications

Natural variations

Experimental info

Preferred order of sections

Cleaved into the following chain:

Molecule processing

Sites

Amino acid modifications

Natural variations

Experimental info

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

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P68250 (1433B_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 72. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
14-3-3 protein beta/alpha

Alternative name(s):
Protein kinase C inhibitor protein 1
Short name=KCIP-1

14-3-3 protein beta/alpha, N-terminally processed

Gene names

Name:

YWHAB

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2 By similarity. Ref.4
Subunit structure	Homodimer. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with YAP1 and KRPCE (phosphorylated form). Interacts with SAMSN1 By similarity. Interacts with PKA-phosphorylated AANAT By similarity. Interacts with the phosphorylated (by AKT1) form of SRPK2 By similarity.
Subcellular location	Cytoplasm. Melanosome By similarity.
Post-translational modification	The alpha, brain-specific form differs from the beta form in being phosphorylated By similarity. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion By similarity.
Sequence similarities	Belongs to the 14-3-3 family.

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative initiation
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic sequestering of protein Inferred from electronic annotation. Source: Compara negative regulation of protein dephosphorylation Inferred from electronic annotation. Source: Compara positive regulation of catalytic activity Inferred from electronic annotation. Source: Compara protein targeting Inferred from sequence or structural similarity. Source: AgBase
Cellular_component	melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: AgBase
Molecular_function	protein domain specific binding Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 246

246

14-3-3 protein beta/alpha

PRO_0000367899

Initiator methionine

Removed; alternate Ref.2

Chain

2 – 246

245

14-3-3 protein beta/alpha, N-terminally processed

PRO_0000000001

Site

Interaction with phosphoserine on interacting protein By similarity

Site

129

Interaction with phosphoserine on interacting protein By similarity

Modified residue

N-acetylmethionine By similarity

Modified residue

N-acetylthreonine; in 14-3-3 protein beta/alpha, N-terminally processed By similarity

Modified residue

Phosphoserine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

117

N6-acetyllysine By similarity

Modified residue

186

Phosphoserine By similarity

Alternative sequence

1 – 2

Missing in isoform Short.

VSP_018631

Sequence conflict

101

Q → E in AAI20113. Ref.1

Sequence		Length	Mass (Da)
Isoform Long [UniParc]. Last modified January 23, 2007. Version 2. Checksum: CABA32314D86800D Show »	FASTA	246	28,081
10 20 30 40 50 60 MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS 70 80 90 100 110 120 WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL QLLDKYLIPN ATQPESKVFY 130 140 150 160 170 180 LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY 190 200 210 220 230 240 YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN « Hide
Isoform Short [UniParc]. Checksum: 6F51C23156F3FE35 Show »	FASTA	244	27,849
10 20 30 40 50 60 MDKSELVQKA KLAEQAERYD DMAAAMKAVT EQGHELSNEE RNLLSVAYKN VVGARRSSWR 70 80 90 100 110 120 VISSIEQKTE RNEKKQQMGK EYREKIEAEL QDICNDVLQL LDKYLIPNAT QPESKVFYLK 130 140 150 160 170 180 MKGDYFRYLS EVASGDNKQT TVSNSQQAYQ EAFEISKKEM QPTHPIRLGL ALNFSVFYYE 190 200 210 220 230 240 ILNSPEKACS LAKTAFDEAI AELDTLNEES YKDSTLIMQL LRDNLTLWTS ENQGDEGDAG EGEN « Hide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Strain: Hereford. Tissue: Fetal pons.
[2]	"Distinct forms of the protein kinase-dependent activator of tyrosine and tryptophan hydroxylases." Isobe T., Ichimura T., Sunaya T., Okuyama T., Takahashi N., Kuwano R., Takahashi Y. J. Mol. Biol. 217:125-132(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-246.
[3]	"Expression of 14-3-3 proteins in bovine retinal photoreceptors." Jones J.M., Niikura T., Pinke R.M., Guo W., Molday L., Leykam J., McConnell D.G. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[4]	"Activation of protein kinase C by purified bovine brain 14-3-3: comparison with tyrosine hydroxylase activation." Tanji M., Horwitz R., Rosenfeld G., Waymire J.C. J. Neurochem. 63:1908-1916(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Sequence databases
EMBL GenBank DDBJ	BC120112 mRNA. Translation: AAI20113.1. AF043736 mRNA. Translation: AAC02090.1.
IPI	IPI00760424. IPI00829553.
PIR	S13467.
RefSeq	NP_777219.2. NM_174794.2.
UniGene	Bt.5535.
3D structure databases
ProteinModelPortal	P68250.
SMR	P68250. Positions 3-233.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000022411.
Proteomic databases
PaxDb	P68250.
PRIDE	P68250.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000022411; ENSBTAP00000022411; ENSBTAG00000016846.
GeneID	286863.
KEGG	bta:286863.
Organism-specific databases
CTD	7529.
Phylogenomic databases
eggNOG	COG5040.
GeneTree	ENSGT00690000101832.
HOGENOM	HOG000240379.
HOVERGEN	HBG050423.
InParanoid	P68250.
KO	K16197.
OrthoDB	EOG4N30PR.
Family and domain databases
Gene3D	1.20.190.20. 1 hit.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806512.

Entry name

1433B_BOVIN

Accession

Primary (citable) accession number: P68250
Secondary accession number(s): P29358, Q0VCL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Note: It is uncertain whether isoform Short is produced by alternative initiation or another biological event.

Isoform Long (identifier: P68250-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P68250-2)

The sequence of this isoform differs from the canonical sequence as follows:
1-2: Missing.

Note: Contains a N-acetylmethionine at position 1 (By similarity).