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Protein

Succinate--CoA ligase [ADP-forming] subunit alpha-1, mitochondrial

Gene

At5g08300

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.UniRule annotation

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (AXX17_At2g15740), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (AXX17_At5g22800), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (At2g20420), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial, Succinate--CoA ligase [ADP-forming] subunit alpha-2, mitochondrial (At5g23250), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (AXX17_At5g08090), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (MKD15.11), Succinate--CoA ligase [ADP-forming] subunit alpha-1, mitochondrial (At5g08300)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92Coenzyme AUniRule annotation1
Binding sitei209Substrate; shared with subunit betaUniRule annotation1
Active sitei298Tele-phosphohistidine intermediateUniRule annotation1

GO - Molecular functioni

  • cofactor binding Source: InterPro
  • copper ion binding Source: TAIR
  • metal ion binding Source: TAIR
  • nucleotide binding Source: UniProtKB-KW
  • succinate-CoA ligase (ADP-forming) activity Source: UniProtKB-EC
  • succinate-CoA ligase (GDP-forming) activity Source: GO_Central

GO - Biological processi

  • response to cadmium ion Source: TAIR
  • tricarboxylic acid cycle Source: UniProtKB-UniPathway

Keywordsi

Molecular functionLigase
Biological processTricarboxylic acid cycle
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G08300-MONOMER.
ReactomeiR-ATH-71403. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00999.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [ADP-forming] subunit alpha-1, mitochondrialUniRule annotation (EC:6.2.1.5UniRule annotation)
Alternative name(s):
Succinyl-CoA synthetase subunit alpha-1UniRule annotation
Short name:
SCS-alpha-1UniRule annotation
Gene namesi
Ordered Locus Names:At5g08300
ORF Names:F8L15_30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G08300.
TAIRilocus:2150773. AT5G08300.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42Mitochondrion2 PublicationsAdd BLAST42
ChainiPRO_000003333543 – 347Succinate--CoA ligase [ADP-forming] subunit alpha-1, mitochondrialAdd BLAST305

Proteomic databases

PaxDbiP68209.
PRIDEiP68209.

PTM databases

iPTMnetiP68209.

Expressioni

Gene expression databases

ExpressionAtlasiP68209. baseline and differential.
GenevisibleiP68209. AT.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.UniRule annotation

Protein-protein interaction databases

STRINGi3702.AT5G08300.1.

Structurei

3D structure databases

ProteinModelPortaliP68209.
SMRiP68209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni66 – 69Coenzyme A bindingUniRule annotation4
Regioni145 – 147Coenzyme A bindingUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase alpha subunit family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1255. Eukaryota.
COG0074. LUCA.
HOGENOMiHOG000239685.
InParanoidiP68209.
KOiK01899.
OMAiEGRTMGH.
OrthoDBiEOG09360EDA.
PhylomeDBiP68209.

Family and domain databases

Gene3Di3.40.50.261. 1 hit.
HAMAPiMF_01988. Succ_CoA_alpha. 1 hit.
InterProiView protein in InterPro
IPR017440. Cit_synth/succinyl-CoA_lig_AS.
IPR033847. Citrt_syn/SCS-alpha_CS.
IPR003781. CoA-bd.
IPR005810. CoA_lig_alpha.
IPR005811. CoA_ligase.
IPR036291. NAD(P)-bd_dom_sf.
IPR016102. Succinyl-CoA_synth-like.
PfamiView protein in Pfam
PF02629. CoA_binding. 1 hit.
PF00549. Ligase_CoA. 1 hit.
PIRSFiPIRSF001553. SucCS_alpha. 1 hit.
SMARTiView protein in SMART
SM00881. CoA_binding. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01019. sucCoAalpha. 1 hit.
PROSITEiView protein in PROSITE
PS01216. SUCCINYL_COA_LIG_1. 1 hit.
PS00399. SUCCINYL_COA_LIG_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68209-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRQVARLIG SLSSKARRCS TGGSEVFPSC QSLTSLTQSR SFASDPHPPA
60 70 80 90 100
AVFVDKNTRV LCQGITGKNG TFHTEQAIEY GTKMVAGVTP KKGGTEHLGL
110 120 130 140 150
PVFNSVAEAK ADTKANASVI YVPAPFAAAA IMEGIEAELD LIVCITEGIP
160 170 180 190 200
QHDMVRVKHA LNSQSKTRLI GPNCPGIIKP GECKIGIMPG YIHKPGKIGI
210 220 230 240 250
VSRSGTLTYE AVFQTTAVGL GQSTCVGIGG DPFNGTNFVD CLEKFFVDPQ
260 270 280 290 300
TEGIVLIGEI GGTAEEDAAA LIKASGTEKP VVAFIAGLTA PPGRRMGHAG
310 320 330 340
AIVSGGKGTA QDKIKSLNDA GVKVVESPAK IGSAMYELFQ ERGLLKQ
Length:347
Mass (Da):36,152
Last modified:October 25, 2004 - v1
Checksum:i0604B07B08FC127D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001807 mRNA. Translation: CAA05023.1.
AL392174 Genomic DNA. Translation: CAC08330.1.
CP002688 Genomic DNA. Translation: AED91279.1.
AY072333 mRNA. Translation: AAL61940.1.
AY114613 mRNA. Translation: AAM47932.1.
AY084298 mRNA. Translation: AAM60889.1.
PIRiT51816.
RefSeqiNP_196447.1. NM_120913.6.
UniGeneiAt.23471.
At.75421.

Genome annotation databases

EnsemblPlantsiAT5G08300.1; AT5G08300.1; AT5G08300.
GeneIDi830726.
GrameneiAT5G08300.1; AT5G08300.1; AT5G08300.
KEGGiath:AT5G08300.

Similar proteinsi

Entry informationi

Entry nameiSUCA1_ARATH
AccessioniPrimary (citable) accession number: P68209
Secondary accession number(s): O82661, P53586
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: October 25, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families