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Protein

Maltose transport system permease protein MalG

Gene

malG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the binding-protein-dependent transport system for maltose; probably responsible for the translocation of the substrate across the membrane.

GO - Molecular functioni

  • maltose-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  • maltodextrin transport Source: EcoCyc
  • maltose transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MALG-MONOMER.
ECOL316407:JW3992-MONOMER.
MetaCyc:MALG-MONOMER.

Protein family/group databases

TCDBi3.A.1.1.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Maltose transport system permease protein MalG
Gene namesi
Name:malG
Ordered Locus Names:b4032, JW3992
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10556. malG.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicCuratedAdd
BLAST
Transmembranei19 – 3921HelicalCuratedAdd
BLAST
Topological domaini40 – 8142PeriplasmicCuratedAdd
BLAST
Transmembranei82 – 10221HelicalCuratedAdd
BLAST
Topological domaini103 – 12321CytoplasmicCuratedAdd
BLAST
Transmembranei124 – 14421HelicalCuratedAdd
BLAST
Topological domaini145 – 1506PeriplasmicCurated
Transmembranei151 – 17121HelicalCuratedAdd
BLAST
Topological domaini172 – 20433CytoplasmicCuratedAdd
BLAST
Transmembranei205 – 22521HelicalCuratedAdd
BLAST
Topological domaini226 – 25934PeriplasmicCuratedAdd
BLAST
Transmembranei260 – 28021HelicalCuratedAdd
BLAST
Topological domaini281 – 29616CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: EcoCyc
  • ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing Source: GO_Central
  • integral component of plasma membrane Source: EcoCyc
  • maltose transport complex Source: EcoCyc
  • membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi190 – 1901E → A, C, K or L: Reduction of transport rate. 2 Publications
Mutagenesisi192 – 1921A → D, S or L: Loss of transport and MalK dissociation from the membrane. 2 Publications
Mutagenesisi196 – 1961G → A: No effect. 2 Publications
Mutagenesisi196 – 1961G → P: Loss of transport and MalK dissociation from the membrane. 2 Publications
Mutagenesisi209 – 2091P → A: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Maltose transport system permease protein MalGPRO_0000060081Add
BLAST

Proteomic databases

PaxDbiP68183.
PRIDEiP68183.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (MalK), two transmembrane proteins (MalG and MalF) and a solute-binding protein (MalE). Protein stability and stable complex formation require YidC.2 Publications

Protein-protein interaction databases

BioGridi4262661. 4 interactions.
DIPiDIP-59709N.
IntActiP68183. 4 interactions.
STRINGi511145.b4032.

Structurei

Secondary structure

1
296
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni7 – 93Combined sources
Helixi12 – 3827Combined sources
Beta strandi40 – 423Combined sources
Beta strandi52 – 543Combined sources
Helixi57 – 626Combined sources
Beta strandi67 – 693Combined sources
Beta strandi70 – 756Combined sources
Helixi81 – 11232Combined sources
Helixi118 – 12912Combined sources
Beta strandi133 – 1353Combined sources
Helixi136 – 14813Combined sources
Helixi152 – 1543Combined sources
Beta strandi155 – 1573Combined sources
Helixi159 – 1668Combined sources
Turni167 – 1704Combined sources
Helixi171 – 18111Combined sources
Helixi187 – 1948Combined sources
Helixi199 – 2057Combined sources
Helixi207 – 22721Combined sources
Helixi231 – 2366Combined sources
Helixi240 – 2423Combined sources
Helixi245 – 2484Combined sources
Helixi249 – 2524Combined sources
Helixi260 – 28021Combined sources
Helixi281 – 2833Combined sources
Turni289 – 2935Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R6GX-ray2.80G1-296[»]
3FH6X-ray4.50G/I1-296[»]
3PUVX-ray2.40G1-296[»]
3PUWX-ray2.30G1-296[»]
3PUXX-ray2.30G1-296[»]
3PUYX-ray3.10G1-296[»]
3PUZX-ray2.90G1-296[»]
3PV0X-ray3.10G1-296[»]
3RLFX-ray2.20G1-296[»]
4JBWX-ray3.91G/I1-296[»]
4KI0X-ray2.38G1-296[»]
ProteinModelPortaliP68183.
SMRiP68183. Positions 11-296.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68183.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 281197ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EG2. Bacteria.
COG3833. LUCA.
HOGENOMiHOG000220526.
InParanoidiP68183.
KOiK10110.
OMAiGIFRYII.
PhylomeDBiP68183.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68183-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMVQPKSQK ARLFITHLLL LLFIAAIMFP LLMVVAISLR QGNFATGSLI
60 70 80 90 100
PEQISWDHWK LALGFSVEQA DGRITPPPFP VLLWLWNSVK VAGISAIGIV
110 120 130 140 150
ALSTTCAYAF ARMRFPGKAT LLKGMLIFQM FPAVLSLVAL YALFDRLGEY
160 170 180 190 200
IPFIGLNTHG GVIFAYLGGI ALHVWTIKGY FETIDSSLEE AAALDGATPW
210 220 230 240 250
QAFRLVLLPL SVPILAVVFI LSFIAAITEV PVASLLLRDV NSYTLAVGMQ
260 270 280 290
QYLNPQNYLW GDFAAAAVMS ALPITIVFLL AQRWLVNGLT AGGVKG
Length:296
Mass (Da):32,225
Last modified:April 1, 1988 - v1
Checksum:iE54418E91E344A29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02871 Genomic DNA. Translation: CAA26628.1.
U00006 Genomic DNA. Translation: AAC43126.1.
U00096 Genomic DNA. Translation: AAC77002.1.
AP009048 Genomic DNA. Translation: BAE78034.1.
J02812 Genomic DNA. Translation: AAA79015.1.
X06663 Genomic DNA. Translation: CAA29861.1.
PIRiA24361. MMECMG.
RefSeqiNP_418456.1. NC_000913.3.
WP_001252058.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77002; AAC77002; b4032.
BAE78034; BAE78034; BAE78034.
GeneIDi948530.
KEGGiecj:JW3992.
eco:b4032.
PATRICi32123597. VBIEscCol129921_4147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02871 Genomic DNA. Translation: CAA26628.1.
U00006 Genomic DNA. Translation: AAC43126.1.
U00096 Genomic DNA. Translation: AAC77002.1.
AP009048 Genomic DNA. Translation: BAE78034.1.
J02812 Genomic DNA. Translation: AAA79015.1.
X06663 Genomic DNA. Translation: CAA29861.1.
PIRiA24361. MMECMG.
RefSeqiNP_418456.1. NC_000913.3.
WP_001252058.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R6GX-ray2.80G1-296[»]
3FH6X-ray4.50G/I1-296[»]
3PUVX-ray2.40G1-296[»]
3PUWX-ray2.30G1-296[»]
3PUXX-ray2.30G1-296[»]
3PUYX-ray3.10G1-296[»]
3PUZX-ray2.90G1-296[»]
3PV0X-ray3.10G1-296[»]
3RLFX-ray2.20G1-296[»]
4JBWX-ray3.91G/I1-296[»]
4KI0X-ray2.38G1-296[»]
ProteinModelPortaliP68183.
SMRiP68183. Positions 11-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262661. 4 interactions.
DIPiDIP-59709N.
IntActiP68183. 4 interactions.
STRINGi511145.b4032.

Protein family/group databases

TCDBi3.A.1.1.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP68183.
PRIDEiP68183.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77002; AAC77002; b4032.
BAE78034; BAE78034; BAE78034.
GeneIDi948530.
KEGGiecj:JW3992.
eco:b4032.
PATRICi32123597. VBIEscCol129921_4147.

Organism-specific databases

EchoBASEiEB0551.
EcoGeneiEG10556. malG.

Phylogenomic databases

eggNOGiENOG4105EG2. Bacteria.
COG3833. LUCA.
HOGENOMiHOG000220526.
InParanoidiP68183.
KOiK10110.
OMAiGIFRYII.
PhylomeDBiP68183.

Enzyme and pathway databases

BioCyciEcoCyc:MALG-MONOMER.
ECOL316407:JW3992-MONOMER.
MetaCyc:MALG-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP68183.
PROiP68183.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMALG_ECOLI
AccessioniPrimary (citable) accession number: P68183
Secondary accession number(s): P07622, Q2M6S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

When MalF EAA loop mutations are made concomitantly with MalG EAA loop mutations, a complete loss of transport and complex formation is observed. This suggests that the MalF-MalG interaction may be important for the proper assembly and also for the correct function of the transporter.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.