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Protein

Maltose transport system permease protein MalG

Gene

malG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the binding-protein-dependent transport system for maltose; probably responsible for the translocation of the substrate across the membrane.

Miscellaneous

When MalF EAA loop mutations are made concomitantly with MalG EAA loop mutations, a complete loss of transport and complex formation is observed. This suggests that the MalF-MalG interaction may be important for the proper assembly and also for the correct function of the transporter.

GO - Molecular functioni

  • maltose-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  • maltodextrin transport Source: EcoCyc
  • maltose transport Source: EcoCyc

Keywordsi

Biological processSugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MALG-MONOMER
MetaCyc:MALG-MONOMER

Protein family/group databases

TCDBi3.A.1.1.1 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Maltose transport system permease protein MalG
Gene namesi
Name:malG
Ordered Locus Names:b4032, JW3992
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10556 malG

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicCuratedAdd BLAST18
Transmembranei19 – 39HelicalCuratedAdd BLAST21
Topological domaini40 – 81PeriplasmicCuratedAdd BLAST42
Transmembranei82 – 102HelicalCuratedAdd BLAST21
Topological domaini103 – 123CytoplasmicCuratedAdd BLAST21
Transmembranei124 – 144HelicalCuratedAdd BLAST21
Topological domaini145 – 150PeriplasmicCurated6
Transmembranei151 – 171HelicalCuratedAdd BLAST21
Topological domaini172 – 204CytoplasmicCuratedAdd BLAST33
Transmembranei205 – 225HelicalCuratedAdd BLAST21
Topological domaini226 – 259PeriplasmicCuratedAdd BLAST34
Transmembranei260 – 280HelicalCuratedAdd BLAST21
Topological domaini281 – 296CytoplasmicCuratedAdd BLAST16

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • maltose transport complex Source: EcoCyc
  • membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi190E → A, C, K or L: Reduction of transport rate. 2 Publications1
Mutagenesisi192A → D, S or L: Loss of transport and MalK dissociation from the membrane. 2 Publications1
Mutagenesisi196G → A: No effect. 2 Publications1
Mutagenesisi196G → P: Loss of transport and MalK dissociation from the membrane. 2 Publications1
Mutagenesisi209P → A: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000600811 – 296Maltose transport system permease protein MalGAdd BLAST296

Proteomic databases

PaxDbiP68183
PRIDEiP68183

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (MalK), two transmembrane proteins (MalG and MalF) and a solute-binding protein (MalE). Protein stability and stable complex formation require YidC.2 Publications

Protein-protein interaction databases

BioGridi4262661, 10 interactors
DIPiDIP-59709N
IntActiP68183, 5 interactors
STRINGi316385.ECDH10B_4221

Structurei

Secondary structure

1296
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni7 – 9Combined sources3
Helixi12 – 38Combined sources27
Beta strandi40 – 42Combined sources3
Beta strandi52 – 54Combined sources3
Helixi57 – 62Combined sources6
Beta strandi67 – 69Combined sources3
Beta strandi70 – 75Combined sources6
Helixi81 – 112Combined sources32
Helixi118 – 129Combined sources12
Beta strandi133 – 135Combined sources3
Helixi136 – 148Combined sources13
Helixi152 – 154Combined sources3
Beta strandi155 – 157Combined sources3
Helixi159 – 166Combined sources8
Turni167 – 170Combined sources4
Helixi171 – 181Combined sources11
Helixi187 – 194Combined sources8
Helixi199 – 205Combined sources7
Helixi207 – 227Combined sources21
Helixi231 – 236Combined sources6
Helixi240 – 242Combined sources3
Helixi245 – 248Combined sources4
Helixi249 – 252Combined sources4
Helixi260 – 280Combined sources21
Helixi281 – 283Combined sources3
Turni289 – 293Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R6GX-ray2.80G1-296[»]
3FH6X-ray4.50G/I1-296[»]
3PUVX-ray2.40G1-296[»]
3PUWX-ray2.30G1-296[»]
3PUXX-ray2.30G1-296[»]
3PUYX-ray3.10G1-296[»]
3PUZX-ray2.90G1-296[»]
3PV0X-ray3.10G1-296[»]
3RLFX-ray2.20G1-296[»]
4JBWX-ray3.91G/I1-296[»]
4KI0X-ray2.38G1-296[»]
ProteinModelPortaliP68183
SMRiP68183
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68183

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 281ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST197

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EG2 Bacteria
COG3833 LUCA
HOGENOMiHOG000220526
InParanoidiP68183
KOiK10110
OMAiFNIWLLK
PhylomeDBiP68183

Family and domain databases

CDDicd06261 TM_PBP2, 1 hit
Gene3Di1.10.3720.10, 1 hit
InterProiView protein in InterPro
IPR000515 MetI-like
IPR035906 MetI-like_sf
PfamiView protein in Pfam
PF00528 BPD_transp_1, 1 hit
SUPFAMiSSF161098 SSF161098, 1 hit
PROSITEiView protein in PROSITE
PS50928 ABC_TM1, 1 hit

Sequencei

Sequence statusi: Complete.

P68183-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMVQPKSQK ARLFITHLLL LLFIAAIMFP LLMVVAISLR QGNFATGSLI
60 70 80 90 100
PEQISWDHWK LALGFSVEQA DGRITPPPFP VLLWLWNSVK VAGISAIGIV
110 120 130 140 150
ALSTTCAYAF ARMRFPGKAT LLKGMLIFQM FPAVLSLVAL YALFDRLGEY
160 170 180 190 200
IPFIGLNTHG GVIFAYLGGI ALHVWTIKGY FETIDSSLEE AAALDGATPW
210 220 230 240 250
QAFRLVLLPL SVPILAVVFI LSFIAAITEV PVASLLLRDV NSYTLAVGMQ
260 270 280 290
QYLNPQNYLW GDFAAAAVMS ALPITIVFLL AQRWLVNGLT AGGVKG
Length:296
Mass (Da):32,225
Last modified:April 1, 1988 - v1
Checksum:iE54418E91E344A29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02871 Genomic DNA Translation: CAA26628.1
U00006 Genomic DNA Translation: AAC43126.1
U00096 Genomic DNA Translation: AAC77002.1
AP009048 Genomic DNA Translation: BAE78034.1
J02812 Genomic DNA Translation: AAA79015.1
X06663 Genomic DNA Translation: CAA29861.1
PIRiA24361 MMECMG
RefSeqiNP_418456.1, NC_000913.3
WP_001252058.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77002; AAC77002; b4032
BAE78034; BAE78034; BAE78034
GeneIDi948530
KEGGiecj:JW3992
eco:b4032
PATRICifig|1411691.4.peg.2679

Similar proteinsi

Entry informationi

Entry nameiMALG_ECOLI
AccessioniPrimary (citable) accession number: P68183
Secondary accession number(s): P07622, Q2M6S2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health