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P68182

- KAPCB_RAT

UniProt

P68182 - KAPCB_RAT

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Protein
cAMP-dependent protein kinase catalytic subunit beta
Gene
Prkacb, Pkacb
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by cAMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei73 – 731ATP By similarity
Active sitei167 – 1671Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi50 – 589ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. cAMP-dependent protein kinase activity Source: UniProtKB
  3. magnesium ion binding Source: UniProtKB
  4. protein kinase A regulatory subunit binding Source: RGD

GO - Biological processi

  1. negative regulation of meiotic cell cycle Source: RGD
  2. protein phosphorylation Source: UniProtKB
  3. response to clozapine Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, cAMP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_196232. Factors involved in megakaryocyte development and platelet production.
REACT_196394. CREB phosphorylation through the activation of Adenylate Cyclase.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase catalytic subunit beta (EC:2.7.11.11)
Short name:
PKA C-beta
Gene namesi
Name:Prkacb
Synonyms:Pkacb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi1310574. Prkacb.

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity. Nucleus By similarity
Note: Translocates into the nucleus (monomeric catalytic subunit) By similarity. The inactive holoenzyme is found in the cytoplasm By similarity.

GO - Cellular componenti

  1. cAMP-dependent protein kinase complex Source: RGD
  2. centrosome Source: Ensembl
  3. cytoplasm Source: RGD
  4. cytosol Source: RGD
  5. nucleus Source: RGD
  6. perinuclear region of cytoplasm Source: RGD
  7. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 351350cAMP-dependent protein kinase catalytic subunit beta
PRO_0000086063Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine By similarity
Modified residuei3 – 31Deamidated asparagine By similarity
Modified residuei11 – 111Phosphoserine By similarity
Modified residuei49 – 491Phosphothreonine By similarity
Modified residuei69 – 691Phosphotyrosine By similarity
Modified residuei140 – 1401Phosphoserine By similarity
Modified residuei196 – 1961Phosphothreonine By similarity
Modified residuei198 – 1981Phosphothreonine By similarity
Modified residuei202 – 2021Phosphothreonine By similarity
Modified residuei331 – 3311Phosphotyrosine By similarity
Modified residuei339 – 3391Phosphoserine By similarity

Post-translational modificationi

Asn-3 is deaminated to Asp in more than 25% of the proteins, giving rise to 2 major isoelectric variants, called CB and CA respectively (0.4 pH unit change). Deamidation proceeds via the so-called beta-aspartyl shift mechanism and yields either 'D-Asp-2' (major) or 'D-isoAsp-2' (minor), in addition to L-isomers. Deamidation occurs after the addition of myristate. The Asn-3 form reaches a significantly larger nuclear/cytoplasmic ratio than the 'Asp-2' form.

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiP68182.
PRIDEiP68182.

PTM databases

PhosphoSiteiP68182.

Expressioni

Gene expression databases

GenevestigatoriP68182.

Interactioni

Subunit structurei

A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The cAMP-dependent protein kinase catalytic subunit binds PJA2 By similarity.

Protein-protein interaction databases

IntActiP68182. 1 interaction.
MINTiMINT-4099447.
STRINGi10116.ENSRNOP00000043572.

Structurei

3D structure databases

ProteinModelPortaliP68182.
SMRiP68182. Positions 15-351.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 298255Protein kinase
Add
BLAST
Domaini299 – 35153AGC-kinase C-terminal
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074358.
HOGENOMiHOG000233033.
HOVERGENiHBG108317.
InParanoidiP68182.
KOiK04345.
OMAiGKEFCEF.
OrthoDBiEOG7VX8WD.
PhylomeDBiP68182.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68182-1 [UniParc]FASTAAdd to Basket

« Hide

MGNTAIAKKG SEVESVKEFL AKAKEDFLRK WENPPPSNAG LEDFERKKTL    50
GTGSFGRVML VKHKATEQYY AMKILDKQKV VKLKQIEHTL NEKRILQAVE 100
FPFLVRLEYS FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ 150
IVLTFEYLHS LDLIYRDLKP ENLLIDHQGY IQVTDFGFAK RVKGRTWTLC 200
GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK 250
IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVS DIKTHKWFAT 300
TDWIAIYQRK VEAPFIPKFR GSGDTSNFDD YEEEEIRVSI TEKCGKEFCE 350
F 351
Length:351
Mass (Da):40,708
Last modified:January 23, 2007 - v2
Checksum:iEBAC9B8041DF9F47
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10770 mRNA. Translation: BAA01601.1.
PIRiJQ1139. OKRTCB.
RefSeqiNP_001071113.1. NM_001077645.1.
UniGeneiRn.202491.

Genome annotation databases

EnsembliENSRNOT00000068739; ENSRNOP00000062228; ENSRNOG00000004978.
GeneIDi293508.
KEGGirno:293508.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10770 mRNA. Translation: BAA01601.1 .
PIRi JQ1139. OKRTCB.
RefSeqi NP_001071113.1. NM_001077645.1.
UniGenei Rn.202491.

3D structure databases

ProteinModelPortali P68182.
SMRi P68182. Positions 15-351.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P68182. 1 interaction.
MINTi MINT-4099447.
STRINGi 10116.ENSRNOP00000043572.

Chemistry

BindingDBi P68182.

PTM databases

PhosphoSitei P68182.

Proteomic databases

PaxDbi P68182.
PRIDEi P68182.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000068739 ; ENSRNOP00000062228 ; ENSRNOG00000004978 .
GeneIDi 293508.
KEGGi rno:293508.

Organism-specific databases

CTDi 5567.
RGDi 1310574. Prkacb.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074358.
HOGENOMi HOG000233033.
HOVERGENi HBG108317.
InParanoidi P68182.
KOi K04345.
OMAi GKEFCEF.
OrthoDBi EOG7VX8WD.
PhylomeDBi P68182.

Enzyme and pathway databases

Reactomei REACT_196232. Factors involved in megakaryocyte development and platelet production.
REACT_196394. CREB phosphorylation through the activation of Adenylate Cyclase.

Miscellaneous databases

NextBioi 636274.

Gene expression databases

Genevestigatori P68182.

Family and domain databases

InterProi IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular structure of the C beta catalytic subunit of rat cAMP-dependent protein kinase and differential expression of C alpha and C beta isoforms in rat tissues and cultured cells."
    Shuntoh H., Sakamoto N., Matsuyama S., Saitoh M., Tanaka C.
    Biochim. Biophys. Acta 1131:175-180(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "A conserved deamidation site at Asn 2 in the catalytic subunit of mammalian cAMP-dependent protein kinase detected by capillary LC-MS and tandem mass spectrometry."
    Jedrzejewski P.T., Girod A., Tholey A., Koenig N., Thullner S., Kinzel V., Bossemeyer D.
    Protein Sci. 7:457-469(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-8, DEAMIDATION AT ASN-3.

Entry informationi

Entry nameiKAPCB_RAT
AccessioniPrimary (citable) accession number: P68182
Secondary accession number(s): P05206
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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