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P68175

- STSY_RAUSE

UniProt

P68175 - STSY_RAUSE

Protein

Strictosidine synthase

Gene

STR1

Organism
Rauvolfia serpentina (Serpentwood) (Devilpepper)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.

    Catalytic activityi

    3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.

    Pathwayi

    GO - Molecular functioni

    1. strictosidine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. alkaloid metabolic process Source: UniProtKB-KW
    2. biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Alkaloid metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00311; UER00447.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Strictosidine synthase (EC:4.3.3.2)
    Gene namesi
    Name:STR1
    OrganismiRauvolfia serpentina (Serpentwood) (Devilpepper)
    Taxonomic identifieri4060 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeRauvolfiinaeRauvolfia

    Subcellular locationi

    GO - Cellular componenti

    1. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222By similarityAdd
    BLAST
    Chaini23 – 344322Strictosidine synthasePRO_0000033334Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed predominantly, but not exclusively, in the root.1 Publication

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    344
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi33 – 375
    Beta strandi39 – 413
    Beta strandi45 – 473
    Beta strandi53 – 586
    Beta strandi62 – 676
    Turni70 – 723
    Beta strandi74 – 807
    Helixi86 – 894
    Helixi95 – 973
    Helixi98 – 1014
    Beta strandi104 – 1107
    Turni111 – 1144
    Beta strandi115 – 1206
    Turni121 – 1233
    Beta strandi124 – 1285
    Beta strandi136 – 1427
    Beta strandi150 – 1556
    Turni157 – 1593
    Beta strandi162 – 1676
    Helixi173 – 1753
    Helixi176 – 1827
    Beta strandi187 – 1926
    Turni194 – 1963
    Beta strandi199 – 2079
    Beta strandi211 – 2144
    Beta strandi218 – 2258
    Turni226 – 2294
    Beta strandi230 – 2389
    Turni239 – 2424
    Beta strandi244 – 2496
    Beta strandi251 – 2588
    Beta strandi264 – 2718
    Beta strandi280 – 2878
    Beta strandi293 – 2986
    Turni301 – 3055
    Beta strandi310 – 3145
    Beta strandi317 – 3215
    Beta strandi326 – 3327

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FP8X-ray2.30A/B23-344[»]
    2FP9X-ray2.96A/B23-344[»]
    2FPBX-ray2.80A/B23-344[»]
    2FPCX-ray3.00A/B23-344[»]
    2V91X-ray3.01A/B32-333[»]
    2VAQX-ray3.01A/B23-344[»]
    3V1SX-ray2.33A/B23-344[»]
    4IMBX-ray2.70A/B32-332[»]
    4IYGX-ray2.70A/B32-333[»]
    ProteinModelPortaliP68175.
    SMRiP68175. Positions 29-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP68175.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the strictosidine synthase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.120.10.30. 1 hit.
    InterProiIPR011042. 6-blade_b-propeller_TolB-like.
    IPR018119. Strictosidine_synth_cons-reg.
    IPR004141. Strictosidine_synthase.
    [Graphical view]
    PANTHERiPTHR10426:SF22. PTHR10426:SF22. 1 hit.
    PfamiPF03088. Str_synth. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P68175-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKLSDSQTM ALFTVFLLFL SSSLALSSPI LKEILIEAPS YAPNSFTFDS    50
    TNKGFYTSVQ DGRVIKYEGP NSGFVDFAYA SPYWNKAFCE NSTDAEKRPL 100
    CGRTYDISYN LQNNQLYIVD CYYHLSVVGS EGGHATQLAT SVDGVPFKWL 150
    YAVTVDQRTG IVYFTDVSTL YDDRGVQQIM DTSDKTGRLI KYDPSTKETT 200
    LLLKELHVPG GAEVSADSSF VLVAEFLSHQ IVKYWLEGPK KGTAEVLVKI 250
    PNPGNIKRNA DGHFWVSSSE ELDGNMHGRV DPKGIKFDEF GNILEVIPLP 300
    PPFAGEHFEQ IQEHDGLLYI GTLFHGSVGI LVYDKKGNSF VSSH 344
    Length:344
    Mass (Da):38,162
    Last modified:October 25, 2004 - v1
    Checksum:i7CD38882621F768A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00756 mRNA. Translation: CAA68725.1.
    X62334 Genomic DNA. Translation: CAA44208.1.
    PIRiS01325.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00756 mRNA. Translation: CAA68725.1 .
    X62334 Genomic DNA. Translation: CAA44208.1 .
    PIRi S01325.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FP8 X-ray 2.30 A/B 23-344 [» ]
    2FP9 X-ray 2.96 A/B 23-344 [» ]
    2FPB X-ray 2.80 A/B 23-344 [» ]
    2FPC X-ray 3.00 A/B 23-344 [» ]
    2V91 X-ray 3.01 A/B 32-333 [» ]
    2VAQ X-ray 3.01 A/B 23-344 [» ]
    3V1S X-ray 2.33 A/B 23-344 [» ]
    4IMB X-ray 2.70 A/B 32-332 [» ]
    4IYG X-ray 2.70 A/B 32-333 [» ]
    ProteinModelPortali P68175.
    SMRi P68175. Positions 29-333.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00311 ; UER00447 .

    Miscellaneous databases

    EvolutionaryTracei P68175.

    Family and domain databases

    Gene3Di 2.120.10.30. 1 hit.
    InterProi IPR011042. 6-blade_b-propeller_TolB-like.
    IPR018119. Strictosidine_synth_cons-reg.
    IPR004141. Strictosidine_synthase.
    [Graphical view ]
    PANTHERi PTHR10426:SF22. PTHR10426:SF22. 1 hit.
    Pfami PF03088. Str_synth. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cDNA clone for strictosidine synthase from Rauvolfia serpentina. DNA sequence determination and expression in Escherichia coli."
      Kutchan T.M., Hampp N., Lottspeich F., Beyreuther K., Zenk M.H.
      FEBS Lett. 237:40-44(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "Strictosidine synthase from Rauvolfia serpentina: analysis of a gene involved in indole alkaloid biosynthesis."
      Bracher D., Kutchan T.M.
      Arch. Biochem. Biophys. 294:717-723(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiSTSY_RAUSE
    AccessioniPrimary (citable) accession number: P68175
    Secondary accession number(s): P15324
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2004
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3