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P68175

- STSY_RAUSE

UniProt

P68175 - STSY_RAUSE

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Protein

Strictosidine synthase

Gene

STR1

Organism
Rauvolfia serpentina (Serpentwood) (Devilpepper)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.

Catalytic activityi

3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.

Pathwayi

GO - Molecular functioni

  1. strictosidine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. alkaloid metabolic process Source: UniProtKB-KW
  2. biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Alkaloid metabolism

Enzyme and pathway databases

UniPathwayiUPA00311; UER00447.

Names & Taxonomyi

Protein namesi
Recommended name:
Strictosidine synthase (EC:4.3.3.2)
Gene namesi
Name:STR1
OrganismiRauvolfia serpentina (Serpentwood) (Devilpepper)
Taxonomic identifieri4060 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeRauvolfiinaeRauvolfia

Subcellular locationi

GO - Cellular componenti

  1. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 344322Strictosidine synthasePRO_0000033334Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed predominantly, but not exclusively, in the root.1 Publication

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
344
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 375
Beta strandi39 – 413
Beta strandi45 – 473
Beta strandi53 – 586
Beta strandi62 – 676
Turni70 – 723
Beta strandi74 – 807
Helixi86 – 894
Helixi95 – 973
Helixi98 – 1014
Beta strandi104 – 1107
Turni111 – 1144
Beta strandi115 – 1206
Turni121 – 1233
Beta strandi124 – 1285
Beta strandi136 – 1427
Beta strandi150 – 1556
Turni157 – 1593
Beta strandi162 – 1676
Helixi173 – 1753
Helixi176 – 1827
Beta strandi187 – 1926
Turni194 – 1963
Beta strandi199 – 2079
Beta strandi211 – 2144
Beta strandi218 – 2258
Turni226 – 2294
Beta strandi230 – 2389
Turni239 – 2424
Beta strandi244 – 2496
Beta strandi251 – 2588
Beta strandi264 – 2718
Beta strandi280 – 2878
Beta strandi293 – 2986
Turni301 – 3055
Beta strandi310 – 3145
Beta strandi317 – 3215
Beta strandi326 – 3327

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FP8X-ray2.30A/B23-344[»]
2FP9X-ray2.96A/B23-344[»]
2FPBX-ray2.80A/B23-344[»]
2FPCX-ray3.00A/B23-344[»]
2V91X-ray3.01A/B32-333[»]
2VAQX-ray3.01A/B23-344[»]
3V1SX-ray2.33A/B23-344[»]
4IMBX-ray2.70A/B32-332[»]
4IYGX-ray2.70A/B32-333[»]
ProteinModelPortaliP68175.
SMRiP68175. Positions 29-333.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68175.

Family & Domainsi

Sequence similaritiesi

Belongs to the strictosidine synthase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426:SF22. PTHR10426:SF22. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68175-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKLSDSQTM ALFTVFLLFL SSSLALSSPI LKEILIEAPS YAPNSFTFDS
60 70 80 90 100
TNKGFYTSVQ DGRVIKYEGP NSGFVDFAYA SPYWNKAFCE NSTDAEKRPL
110 120 130 140 150
CGRTYDISYN LQNNQLYIVD CYYHLSVVGS EGGHATQLAT SVDGVPFKWL
160 170 180 190 200
YAVTVDQRTG IVYFTDVSTL YDDRGVQQIM DTSDKTGRLI KYDPSTKETT
210 220 230 240 250
LLLKELHVPG GAEVSADSSF VLVAEFLSHQ IVKYWLEGPK KGTAEVLVKI
260 270 280 290 300
PNPGNIKRNA DGHFWVSSSE ELDGNMHGRV DPKGIKFDEF GNILEVIPLP
310 320 330 340
PPFAGEHFEQ IQEHDGLLYI GTLFHGSVGI LVYDKKGNSF VSSH
Length:344
Mass (Da):38,162
Last modified:October 25, 2004 - v1
Checksum:i7CD38882621F768A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00756 mRNA. Translation: CAA68725.1.
X62334 Genomic DNA. Translation: CAA44208.1.
PIRiS01325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00756 mRNA. Translation: CAA68725.1 .
X62334 Genomic DNA. Translation: CAA44208.1 .
PIRi S01325.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FP8 X-ray 2.30 A/B 23-344 [» ]
2FP9 X-ray 2.96 A/B 23-344 [» ]
2FPB X-ray 2.80 A/B 23-344 [» ]
2FPC X-ray 3.00 A/B 23-344 [» ]
2V91 X-ray 3.01 A/B 32-333 [» ]
2VAQ X-ray 3.01 A/B 23-344 [» ]
3V1S X-ray 2.33 A/B 23-344 [» ]
4IMB X-ray 2.70 A/B 32-332 [» ]
4IYG X-ray 2.70 A/B 32-333 [» ]
ProteinModelPortali P68175.
SMRi P68175. Positions 29-333.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00311 ; UER00447 .

Miscellaneous databases

EvolutionaryTracei P68175.

Family and domain databases

Gene3Di 2.120.10.30. 1 hit.
InterProi IPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view ]
PANTHERi PTHR10426:SF22. PTHR10426:SF22. 1 hit.
Pfami PF03088. Str_synth. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The cDNA clone for strictosidine synthase from Rauvolfia serpentina. DNA sequence determination and expression in Escherichia coli."
    Kutchan T.M., Hampp N., Lottspeich F., Beyreuther K., Zenk M.H.
    FEBS Lett. 237:40-44(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Strictosidine synthase from Rauvolfia serpentina: analysis of a gene involved in indole alkaloid biosynthesis."
    Bracher D., Kutchan T.M.
    Arch. Biochem. Biophys. 294:717-723(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSTSY_RAUSE
AccessioniPrimary (citable) accession number: P68175
Secondary accession number(s): P15324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3