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Protein

Strictosidine synthase

Gene

STR1

Organism
Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.

Catalytic activityi

3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.

Pathwayi: 3alpha(S)-strictosidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 3alpha(S)-strictosidine from secologanin and tryptamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Strictosidine synthase (STR1)
This subpathway is part of the pathway 3alpha(S)-strictosidine biosynthesis, which is itself part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3alpha(S)-strictosidine from secologanin and tryptamine, the pathway 3alpha(S)-strictosidine biosynthesis and in Alkaloid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Alkaloid metabolism

Enzyme and pathway databases

BRENDAi4.3.3.2. 5309.
UniPathwayiUPA00311; UER00447.

Names & Taxonomyi

Protein namesi
Recommended name:
Strictosidine synthase (EC:4.3.3.2)
Gene namesi
Name:STR1
OrganismiRauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum)
Taxonomic identifieri4060 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeRauvolfiinaeRauvolfia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000003333423 – 344Strictosidine synthaseAdd BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi91N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed predominantly, but not exclusively, in the root.1 Publication

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1344
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 37Combined sources5
Beta strandi39 – 41Combined sources3
Beta strandi45 – 47Combined sources3
Beta strandi53 – 58Combined sources6
Beta strandi62 – 67Combined sources6
Turni70 – 72Combined sources3
Beta strandi74 – 80Combined sources7
Helixi86 – 89Combined sources4
Helixi95 – 97Combined sources3
Helixi98 – 101Combined sources4
Beta strandi104 – 110Combined sources7
Turni111 – 114Combined sources4
Beta strandi115 – 120Combined sources6
Turni121 – 123Combined sources3
Beta strandi124 – 128Combined sources5
Beta strandi136 – 142Combined sources7
Beta strandi150 – 155Combined sources6
Turni157 – 159Combined sources3
Beta strandi162 – 167Combined sources6
Helixi173 – 175Combined sources3
Helixi176 – 182Combined sources7
Beta strandi187 – 192Combined sources6
Turni194 – 196Combined sources3
Beta strandi199 – 207Combined sources9
Beta strandi211 – 214Combined sources4
Beta strandi218 – 225Combined sources8
Turni226 – 229Combined sources4
Beta strandi230 – 238Combined sources9
Turni239 – 242Combined sources4
Beta strandi244 – 249Combined sources6
Beta strandi251 – 258Combined sources8
Beta strandi264 – 271Combined sources8
Beta strandi280 – 287Combined sources8
Beta strandi293 – 298Combined sources6
Turni301 – 305Combined sources5
Beta strandi310 – 314Combined sources5
Beta strandi317 – 321Combined sources5
Beta strandi326 – 332Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FP8X-ray2.30A/B23-344[»]
2FP9X-ray2.96A/B23-344[»]
2FPBX-ray2.80A/B23-344[»]
2FPCX-ray3.00A/B23-344[»]
2V91X-ray3.01A/B32-333[»]
2VAQX-ray3.01A/B23-344[»]
3V1SX-ray2.33A/B23-344[»]
4IMBX-ray2.70A/B32-332[»]
4IYGX-ray2.70A/B32-333[»]
ProteinModelPortaliP68175.
SMRiP68175.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68175.

Family & Domainsi

Sequence similaritiesi

Belongs to the strictosidine synthase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426. PTHR10426. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLSDSQTM ALFTVFLLFL SSSLALSSPI LKEILIEAPS YAPNSFTFDS
60 70 80 90 100
TNKGFYTSVQ DGRVIKYEGP NSGFVDFAYA SPYWNKAFCE NSTDAEKRPL
110 120 130 140 150
CGRTYDISYN LQNNQLYIVD CYYHLSVVGS EGGHATQLAT SVDGVPFKWL
160 170 180 190 200
YAVTVDQRTG IVYFTDVSTL YDDRGVQQIM DTSDKTGRLI KYDPSTKETT
210 220 230 240 250
LLLKELHVPG GAEVSADSSF VLVAEFLSHQ IVKYWLEGPK KGTAEVLVKI
260 270 280 290 300
PNPGNIKRNA DGHFWVSSSE ELDGNMHGRV DPKGIKFDEF GNILEVIPLP
310 320 330 340
PPFAGEHFEQ IQEHDGLLYI GTLFHGSVGI LVYDKKGNSF VSSH
Length:344
Mass (Da):38,162
Last modified:October 25, 2004 - v1
Checksum:i7CD38882621F768A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00756 mRNA. Translation: CAA68725.1.
X62334 Genomic DNA. Translation: CAA44208.1.
PIRiS01325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00756 mRNA. Translation: CAA68725.1.
X62334 Genomic DNA. Translation: CAA44208.1.
PIRiS01325.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FP8X-ray2.30A/B23-344[»]
2FP9X-ray2.96A/B23-344[»]
2FPBX-ray2.80A/B23-344[»]
2FPCX-ray3.00A/B23-344[»]
2V91X-ray3.01A/B32-333[»]
2VAQX-ray3.01A/B23-344[»]
3V1SX-ray2.33A/B23-344[»]
4IMBX-ray2.70A/B32-332[»]
4IYGX-ray2.70A/B32-333[»]
ProteinModelPortaliP68175.
SMRiP68175.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00311; UER00447.
BRENDAi4.3.3.2. 5309.

Miscellaneous databases

EvolutionaryTraceiP68175.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426. PTHR10426. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTSY_RAUSE
AccessioniPrimary (citable) accession number: P68175
Secondary accession number(s): P15324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.