Reviewed, UniProtKB/Swiss-Prot P68175 (STSY_RAUSE)

Names and origin

Protein names

Recommended name:
    Strictosidine synthase
    EC=4.3.3.2

Gene names

Name:

STR1

Organism

Rauvolfia serpentina (Serpentwood) (Devilpepper)

Taxonomic identifier

4060 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Rauvolfia

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.
Catalytic activity	3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.
Pathway	Alkaloid biosynthesis; 3alpha(S)-strictosidine biosynthesis; 3alpha(S)-strictosidine from secologanin and tryptamine: step 1/1.
Subunit structure	Monomer.
Subcellular location	Vacuole.
Tissue specificity	Expressed predominantly, but not exclusively, in the root. Ref.2
Sequence similarities	Belongs to the strictosidine synthase family.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Cellular component	Vacuole
Domain	Signal
Molecular function	Lyase
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	strictosidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

By similarity

Chain

23 – 344

322

Strictosidine synthase

PRO_0000033334

Amino acid modifications

Glycosylation

91

1

N-linked (GlcNAc...) Potential

Secondary structure

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344

Helix Strand Turn

Details...

Beta strand

33 – 37

5

Beta strand

39 – 41

3

Beta strand

53 – 58

6

Beta strand

62 – 67

6

Turn

70 – 72

3

Beta strand

74 – 80

7

Helix

86 – 89

4

Helix

95 – 97

3

Helix

98 – 101

4

Beta strand

104 – 110

7

Turn

111 – 114

4

Beta strand

115 – 120

6

Turn

121 – 123

3

Beta strand

124 – 128

5

Beta strand

136 – 142

7

Beta strand

150 – 155

6

Turn

157 – 159

3

Beta strand

162 – 167

6

Helix

176 – 182

7

Beta strand

187 – 192

6

Turn

194 – 196

3

Beta strand

199 – 207

9

Beta strand

211 – 214

4

Beta strand

218 – 225

8

Turn

226 – 229

4

Beta strand

230 – 238

9

Turn

239 – 242

4

Beta strand

244 – 249

6

Beta strand

251 – 258

8

Beta strand

264 – 271

8

Beta strand

280 – 287

8

Beta strand

293 – 298

6

Turn

301 – 305

5

Beta strand

310 – 314

5

Beta strand

317 – 321

5

Beta strand

326 – 332

7

Sequences

	Sequence		Length	Mass (Da)
	P68175-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 7CD38882621F768A	FASTA	344	38,162
	10 20 30 40 50 60 MAKLSDSQTM ALFTVFLLFL SSSLALSSPI LKEILIEAPS YAPNSFTFDS TNKGFYTSVQ 70 80 90 100 110 120 DGRVIKYEGP NSGFVDFAYA SPYWNKAFCE NSTDAEKRPL CGRTYDISYN LQNNQLYIVD 130 140 150 160 170 180 CYYHLSVVGS EGGHATQLAT SVDGVPFKWL YAVTVDQRTG IVYFTDVSTL YDDRGVQQIM 190 200 210 220 230 240 DTSDKTGRLI KYDPSTKETT LLLKELHVPG GAEVSADSSF VLVAEFLSHQ IVKYWLEGPK 250 260 270 280 290 300 KGTAEVLVKI PNPGNIKRNA DGHFWVSSSE ELDGNMHGRV DPKGIKFDEF GNILEVIPLP 310 320 330 340 PPFAGEHFEQ IQEHDGLLYI GTLFHGSVGI LVYDKKGNSF VSSH

References

[1]	"The cDNA clone for strictosidine synthase from Rauvolfia serpentina. DNA sequence determination and expression in Escherichia coli." Kutchan T.M., Hampp N., Lottspeich F., Beyreuther K., Zenk M.H. FEBS Lett. 237:40-44(1988) [PubMed: 3049153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Strictosidine synthase from Rauvolfia serpentina: analysis of a gene involved in indole alkaloid biosynthesis." Bracher D., Kutchan T.M. Arch. Biochem. Biophys. 294:717-723(1992) [PubMed: 1567228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y00756 mRNA. Translation: CAA68725.1.
X62334 Genomic DNA. Translation: CAA44208.1.

PIR

S01325.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FP8	X-ray	2.30	A/B	23-344	[»]
2FP9	X-ray	2.96	A/B	23-344	[»]
2FPB	X-ray	2.80	A/B	23-344	[»]
2FPC	X-ray	3.00	A/B	23-344	[»]
2V91	X-ray	3.01	A/B	32-333	[»]
2VAQ	X-ray	3.01	A/B	23-344	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

4.3.3.2. 257570.

Family and domain databases

InterPro

IPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]

Gene3D

G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.

PANTHER

PTHR10426. Str_synth. 1 hit.

Pfam

PF03088. Str_synth. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

STSY_RAUSE

Accession

Primary (citable) accession number: P68175
Secondary accession number(s): P15324

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	September 22, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)