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Protein

Strictosidine synthase

Gene

STR1

Organism
Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.

Catalytic activityi

3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.

Pathwayi

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Alkaloid metabolism

Enzyme and pathway databases

BRENDAi4.3.3.2. 5309.
UniPathwayiUPA00311; UER00447.

Names & Taxonomyi

Protein namesi
Recommended name:
Strictosidine synthase (EC:4.3.3.2)
Gene namesi
Name:STR1
OrganismiRauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum)
Taxonomic identifieri4060 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeRauvolfiinaeRauvolfia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 344322Strictosidine synthasePRO_0000033334Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed predominantly, but not exclusively, in the root.1 Publication

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
344
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 375Combined sources
Beta strandi39 – 413Combined sources
Beta strandi45 – 473Combined sources
Beta strandi53 – 586Combined sources
Beta strandi62 – 676Combined sources
Turni70 – 723Combined sources
Beta strandi74 – 807Combined sources
Helixi86 – 894Combined sources
Helixi95 – 973Combined sources
Helixi98 – 1014Combined sources
Beta strandi104 – 1107Combined sources
Turni111 – 1144Combined sources
Beta strandi115 – 1206Combined sources
Turni121 – 1233Combined sources
Beta strandi124 – 1285Combined sources
Beta strandi136 – 1427Combined sources
Beta strandi150 – 1556Combined sources
Turni157 – 1593Combined sources
Beta strandi162 – 1676Combined sources
Helixi173 – 1753Combined sources
Helixi176 – 1827Combined sources
Beta strandi187 – 1926Combined sources
Turni194 – 1963Combined sources
Beta strandi199 – 2079Combined sources
Beta strandi211 – 2144Combined sources
Beta strandi218 – 2258Combined sources
Turni226 – 2294Combined sources
Beta strandi230 – 2389Combined sources
Turni239 – 2424Combined sources
Beta strandi244 – 2496Combined sources
Beta strandi251 – 2588Combined sources
Beta strandi264 – 2718Combined sources
Beta strandi280 – 2878Combined sources
Beta strandi293 – 2986Combined sources
Turni301 – 3055Combined sources
Beta strandi310 – 3145Combined sources
Beta strandi317 – 3215Combined sources
Beta strandi326 – 3327Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FP8X-ray2.30A/B23-344[»]
2FP9X-ray2.96A/B23-344[»]
2FPBX-ray2.80A/B23-344[»]
2FPCX-ray3.00A/B23-344[»]
2V91X-ray3.01A/B32-333[»]
2VAQX-ray3.01A/B23-344[»]
3V1SX-ray2.33A/B23-344[»]
4IMBX-ray2.70A/B32-332[»]
4IYGX-ray2.70A/B32-333[»]
ProteinModelPortaliP68175.
SMRiP68175. Positions 29-333.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68175.

Family & Domainsi

Sequence similaritiesi

Belongs to the strictosidine synthase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426:SF22. PTHR10426:SF22. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLSDSQTM ALFTVFLLFL SSSLALSSPI LKEILIEAPS YAPNSFTFDS
60 70 80 90 100
TNKGFYTSVQ DGRVIKYEGP NSGFVDFAYA SPYWNKAFCE NSTDAEKRPL
110 120 130 140 150
CGRTYDISYN LQNNQLYIVD CYYHLSVVGS EGGHATQLAT SVDGVPFKWL
160 170 180 190 200
YAVTVDQRTG IVYFTDVSTL YDDRGVQQIM DTSDKTGRLI KYDPSTKETT
210 220 230 240 250
LLLKELHVPG GAEVSADSSF VLVAEFLSHQ IVKYWLEGPK KGTAEVLVKI
260 270 280 290 300
PNPGNIKRNA DGHFWVSSSE ELDGNMHGRV DPKGIKFDEF GNILEVIPLP
310 320 330 340
PPFAGEHFEQ IQEHDGLLYI GTLFHGSVGI LVYDKKGNSF VSSH
Length:344
Mass (Da):38,162
Last modified:October 25, 2004 - v1
Checksum:i7CD38882621F768A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00756 mRNA. Translation: CAA68725.1.
X62334 Genomic DNA. Translation: CAA44208.1.
PIRiS01325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00756 mRNA. Translation: CAA68725.1.
X62334 Genomic DNA. Translation: CAA44208.1.
PIRiS01325.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FP8X-ray2.30A/B23-344[»]
2FP9X-ray2.96A/B23-344[»]
2FPBX-ray2.80A/B23-344[»]
2FPCX-ray3.00A/B23-344[»]
2V91X-ray3.01A/B32-333[»]
2VAQX-ray3.01A/B23-344[»]
3V1SX-ray2.33A/B23-344[»]
4IMBX-ray2.70A/B32-332[»]
4IYGX-ray2.70A/B32-333[»]
ProteinModelPortaliP68175.
SMRiP68175. Positions 29-333.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00311; UER00447.
BRENDAi4.3.3.2. 5309.

Miscellaneous databases

EvolutionaryTraceiP68175.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426:SF22. PTHR10426:SF22. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The cDNA clone for strictosidine synthase from Rauvolfia serpentina. DNA sequence determination and expression in Escherichia coli."
    Kutchan T.M., Hampp N., Lottspeich F., Beyreuther K., Zenk M.H.
    FEBS Lett. 237:40-44(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Strictosidine synthase from Rauvolfia serpentina: analysis of a gene involved in indole alkaloid biosynthesis."
    Bracher D., Kutchan T.M.
    Arch. Biochem. Biophys. 294:717-723(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSTSY_RAUSE
AccessioniPrimary (citable) accession number: P68175
Secondary accession number(s): P15324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: April 1, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.