Reviewed, UniProtKB/Swiss-Prot P68174 (STSY_RAUMA)

Names and origin

Protein names

Recommended name:
    Strictosidine synthase
    EC=4.3.3.2

Gene names

Name:

STR1

Organism

Rauvolfia mannii

Taxonomic identifier

4062 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Rauvolfia

Protein attributes

Sequence length	342 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.
Catalytic activity	3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.
Pathway	Alkaloid biosynthesis; 3alpha(S)-strictosidine biosynthesis; 3alpha(S)-strictosidine from secologanin and tryptamine: step 1/1.
Subunit structure	Monomer.
Subcellular location	Vacuole.
Sequence similarities	Belongs to the strictosidine synthase family.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Cellular component	Vacuole
Domain	Signal
Molecular function	Lyase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	strictosidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 20	›20	By similarity
	Chain	21 – 342	322	Strictosidine synthase		PRO_0000033333
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P68174-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 61F4B51826E269E8	FASTA	342	37,960
	10 20 30 40 50 60 KLSDSQTMAL FTVFLLFLSS SLALSSPILK EILIEAPSYA PNSFTFDSTN KGFYTSVQDG 70 80 90 100 110 120 RVIKYEGPNS GFVDFAYASP YWNKAFCENS TDAEKRPLCG RTYDISYNLQ NNQLYIVDCY 130 140 150 160 170 180 YHLSVVGSEG GHATQLATSV DGVPFKWLYA VTVDQRTGIV YFTDVSTLYD DRGVQQIMDT 190 200 210 220 230 240 SDKTGRLIKY DPSTKETTLL LKELHVPGGA EVSADSSFVL VAEFLSHQIV KYWLEGPKKG 250 260 270 280 290 300 TAEVLVKIPN PGNIKRNADG HFWVSSSEEL DGNMHGRVDP KGIKFDEFGN ILEVIPLPPP 310 320 330 340 FAGEHFEQIQ EHDGLLYIGT LFHGSVGILV YDKKGNSFVS SH

References

[1]	"Strictosidine synthase from Rauvolfia serpentina: analysis of a gene involved in indole alkaloid biosynthesis." Bracher D., Kutchan T.M. Arch. Biochem. Biophys. 294:717-723(1992) [PubMed: 1567228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X63431 Genomic DNA. Translation: CAA45025.1.
PIR	S29894.
3D structure databases
SMR	P68174. Positions 29-331.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.3.3.2. 261291.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR018119. Strictosidine_synth_cons-reg. IPR004141. Strictosidine_synthase. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
PANTHER	PTHR10426. Str_synth. 1 hit.
Pfam	PF03088. Str_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STSY_RAUMA

Accession

Primary (citable) accession number: P68174
Secondary accession number(s): P15324

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	September 22, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)