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Protein

Strictosidine synthase

Gene

STR1

Organism
Rauvolfia mannii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.

Catalytic activityi

3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.

Pathwayi

GO - Molecular functioni

  1. strictosidine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. alkaloid metabolic process Source: UniProtKB-KW
  2. biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Alkaloid metabolism

Enzyme and pathway databases

UniPathwayiUPA00311; UER00447.

Names & Taxonomyi

Protein namesi
Recommended name:
Strictosidine synthase (EC:4.3.3.2)
Gene namesi
Name:STR1
OrganismiRauvolfia mannii
Taxonomic identifieri4062 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeRauvolfiinaeRauvolfia

Subcellular locationi

GO - Cellular componenti

  1. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 20›20By similarityAdd
BLAST
Chaini21 – 342322Strictosidine synthasePRO_0000033333Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP68174.
SMRiP68174. Positions 27-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the strictosidine synthase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426:SF22. PTHR10426:SF22. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
KLSDSQTMAL FTVFLLFLSS SLALSSPILK EILIEAPSYA PNSFTFDSTN
60 70 80 90 100
KGFYTSVQDG RVIKYEGPNS GFVDFAYASP YWNKAFCENS TDAEKRPLCG
110 120 130 140 150
RTYDISYNLQ NNQLYIVDCY YHLSVVGSEG GHATQLATSV DGVPFKWLYA
160 170 180 190 200
VTVDQRTGIV YFTDVSTLYD DRGVQQIMDT SDKTGRLIKY DPSTKETTLL
210 220 230 240 250
LKELHVPGGA EVSADSSFVL VAEFLSHQIV KYWLEGPKKG TAEVLVKIPN
260 270 280 290 300
PGNIKRNADG HFWVSSSEEL DGNMHGRVDP KGIKFDEFGN ILEVIPLPPP
310 320 330 340
FAGEHFEQIQ EHDGLLYIGT LFHGSVGILV YDKKGNSFVS SH
Length:342
Mass (Da):37,960
Last modified:October 25, 2004 - v1
Checksum:i61F4B51826E269E8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63431 Genomic DNA. Translation: CAA45025.1.
PIRiS29894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63431 Genomic DNA. Translation: CAA45025.1.
PIRiS29894.

3D structure databases

ProteinModelPortaliP68174.
SMRiP68174. Positions 27-331.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00311; UER00447.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426:SF22. PTHR10426:SF22. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Strictosidine synthase from Rauvolfia serpentina: analysis of a gene involved in indole alkaloid biosynthesis."
    Bracher D., Kutchan T.M.
    Arch. Biochem. Biophys. 294:717-723(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiSTSY_RAUMA
AccessioniPrimary (citable) accession number: P68174
Secondary accession number(s): P15324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: January 7, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.