P68173 (SAHH_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosylhomocysteinase
Short name=AdoHcyase
EC=3.3.1.1

Alternative name(s):
Cytokinin-binding protein CBP57
S-adenosyl-L-homocysteine hydrolase

Gene names

Name:

SAHH

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine By similarity.
Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NAD
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Adenosylhomocysteinase		PRO_0000116932
Regions
	Nucleotide binding	206 – 208	3	NAD By similarity
	Nucleotide binding	269 – 274	6	NAD By similarity
	Nucleotide binding	348 – 350	3	NAD By similarity
Sites
	Binding site	64	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	205	1	Substrate By similarity
	Binding site	235	1	Substrate By similarity
	Binding site	239	1	Substrate By similarity
	Binding site	240	1	NAD By similarity
	Binding site	292	1	NAD By similarity
	Binding site	327	1	NAD By similarity
	Binding site	397	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68173 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: AA6D6844E9DF0A5C	FASTA	485	53,104
	10 20 30 40 50 60 MALLVEKTTS GREYKVKDMS QADFGRLEIE LAEVEMPGLM ACRTEFGPSQ PFKGAKITGS 70 80 90 100 110 120 LHMTIQTAVL IETLTALGAE VRWCSCNIFS TQDHAAAAIA RDSAAVFAWK GETLQEYWWC 130 140 150 160 170 180 TERALDWGPG GGPDLIVDDG GDATLLIHEG VKAEEEFAKN GTIPDPNSTD NAEFQLVLTI 190 200 210 220 230 240 IKESLKTDPL KYTKMKERLV GVSEETTTGV KRLYQMQANG TLLFPAINVN DSVTKSKFDN 250 260 270 280 290 300 LYGCRHSLPD GLMRATDVMI AGKVALVAGY GDVGKGCAAA LKQAGARVIV TEIDPICALQ 310 320 330 340 350 360 ATMEGLQVLT LEDVVSDVDI FVTTTGNKDI IMVDHMRKMK NNAIVCNIGH FDNEIDMLGL 370 380 390 400 410 420 ETYPGVKRIT IKPQTDRWVF PDTNSGIIVL AEGRLMNLGC ATGHPSFVMS CSFTNQVIAQ 430 440 450 460 470 480 LELWNEKSSG KYEKKVYVLP KHLDEKVAAL HLGKLGAKLT KLSKDQADYI SVPVEGPYKP AHYRY

References

[1]	"Inducible expression by plant hormones of S-adenosyl-homocysteine hydrolase gene from Nicotiana tabacum during early flower bud formation in vitro." Tanaka H., Masuta C., Kataoka J., Kuwata S., Koiwai A., Noma M. Plant Sci. 113:167-174(1996) Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Bright Yellow 4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D45204 mRNA. Translation: BAA08142.1. D49804 Genomic DNA. Translation: BAA23164.1.
3D structure databases
ProteinModelPortal	P68173.
SMR	P68173. Positions 12-485.
ModBase	Search...
Proteomic databases
PRIDE	P68173.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000043. Adenosylhomocysteinase. IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMART	SM00996. AdoHcyase. 1 hit. SM00997. AdoHcyase_NAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00936. AhcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_TOBAC

Accession

Primary (citable) accession number: P68173
Secondary accession number(s): P50248

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program