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Protein

Non-legume hemoglobin

Gene
N/A
Organism
Parasponia andersonii (Sponia andersonii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Provides oxygen to the bacteroids. This role is essential for symbiotic nitrogen fixation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron (heme distal ligand)PROSITE-ProRule annotation
Metal bindingi105 – 1051Iron (heme proximal ligand)PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nitrogen fixation, Oxygen transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-legume hemoglobin
OrganismiParasponia andersonii (Sponia andersonii)
Taxonomic identifieri3476 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesCannabaceaeParasponia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 162161Non-legume hemoglobinPRO_0000192981Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine

Keywords - PTMi

Acetylation

Expressioni

Tissue specificityi

Root nodules.

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
162
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 2514Combined sources
Helixi26 – 283Combined sources
Helixi29 – 4315Combined sources
Helixi45 – 506Combined sources
Helixi52 – 543Combined sources
Helixi67 – 8822Combined sources
Helixi95 – 10814Combined sources
Helixi112 – 12918Combined sources
Turni131 – 1333Combined sources
Helixi136 – 15419Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QQRX-ray2.16A/B1-162[»]
ProteinModelPortaliP68168.
SMRiP68168. Positions 9-159.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68168.

Family & Domainsi

Sequence similaritiesi

Belongs to the plant globin family.Curated

Family and domain databases

Gene3Di1.10.490.10. 1 hit.
InterProiIPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin/Proto.
IPR001032. Leghaemoglobin.
IPR019824. Leghaemoglobin_Fe_BS.
[Graphical view]
PfamiPF00042. Globin. 1 hit.
[Graphical view]
PRINTSiPR00188. PLANTGLOBIN.
SUPFAMiSSF46458. SSF46458. 1 hit.
PROSITEiPS01033. GLOBIN. 1 hit.
PS00208. PLANT_GLOBIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSEVNKVF TEEQEALVVK AWAVMKKNSA ELGLQFFLKI FEIAPSAKNL
60 70 80 90 100
FSYLKDSPVP LEQNPKLKPH ATTVFVMTCE SAVQLRKAGK ATVKESDLKR
110 120 130 140 150
IGAIHFKTGV VNEHFEVTRF ALLETIKEAV PEMWSPEMKN AWGVAYDQLV
160
AAIKFEMKPS ST
Length:162
Mass (Da):18,150
Last modified:January 23, 2007 - v2
Checksum:iC68B7F8F47E3DFB4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911A → V in AAB86653 (Ref. 1) Curated

Polymorphismi

Two variants of the protein, hemoglobin I and hemoglobin II seem to exist.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti31 – 311E → D Polymorphism; in 30% hemoglobin Iin 30% hemoglobin I. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27194 Genomic DNA. Translation: AAB86653.1.
PIRiS05541. GPDRNL.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27194 Genomic DNA. Translation: AAB86653.1.
PIRiS05541. GPDRNL.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QQRX-ray2.16A/B1-162[»]
ProteinModelPortaliP68168.
SMRiP68168. Positions 9-159.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP68168.

Family and domain databases

Gene3Di1.10.490.10. 1 hit.
InterProiIPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin/Proto.
IPR001032. Leghaemoglobin.
IPR019824. Leghaemoglobin_Fe_BS.
[Graphical view]
PfamiPF00042. Globin. 1 hit.
[Graphical view]
PRINTSiPR00188. PLANTGLOBIN.
SUPFAMiSSF46458. SSF46458. 1 hit.
PROSITEiPS01033. GLOBIN. 1 hit.
PS00208. PLANT_GLOBIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Common evolutionary origin of legume and non-legume plant haemoglobins."
    Landsmann J., Dennis E.S., Higgins T.J.V., Appleby C.A., Kortt A.A., Peacock W.J.
    Nature 324:166-168(1986)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The amino acid sequence of hemoglobin I from Parasponia andersonii, a nonleguminous plant."
    Kortt A.A., Burns J.E., Trinick M.J., Appleby C.A.
    FEBS Lett. 180:55-60(1985)
    Cited for: PROTEIN SEQUENCE OF 6-160.
  3. "Amino acid sequences of hemoglobins I and II from root nodules of the non-leguminous Parasponia rigida-rhizobium symbiosis, and a correction of the sequence of hemoglobin I from Parasponia andersonii."
    Kortt A.A., Trinick M.J., Appleby C.A.
    Eur. J. Biochem. 175:141-149(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-40 AND 67-86, SEQUENCE REVISION, POLYMORPHISM, VARIANT ASP-31.

Entry informationi

Entry nameiHBPL_PARAD
AccessioniPrimary (citable) accession number: P68168
Secondary accession number(s): P04396
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.