Reviewed, UniProtKB/Swiss-Prot P68136 (ACTS_RAT)

Names and origin

Protein names

Recommended name:
    Actin, alpha skeletal muscle
Alternative name(s):
    Alpha-actin-1

Gene names

Name:	Acta1
Synonyms:	Acta

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit structure	Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID By similarity.
Subcellular location	Cytoplasm › cytoskeleton.
Miscellaneous	In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Sequence similarities	Belongs to the actin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	ATP-binding Nucleotide-binding
Molecular function	Muscle protein
PTM	Acetylation Methylation Phosphoprotein
Gene Ontology (GO)
Biological process	cell growth Inferred from expression pattern. Source: RGD muscle thin filament assembly Inferred from sequence or structural similarity. Source: UniProtKB response to extracellular stimulus Inferred from expression pattern. Source: RGD response to long exposure to lithium ion Inferred from expression pattern. Source: RGD response to mechanical stimulus Inferred from expression pattern. Source: RGD response to steroid hormone stimulus Inferred from expression pattern. Source: RGD skeletal muscle fiber adaptation Inferred from expression pattern. Source: RGD skeletal muscle fiber development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	actin filament Inferred from sequence or structural similarity. Source: UniProtKB stress fiber Inferred from sequence or structural similarity. Source: UniProtKB striated muscle thin filament Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 2	2	Removed in mature form By similarity		PRO_0000000852
	Chain	3 – 377	375	Actin, alpha skeletal muscle		PRO_0000000853
Amino acid modifications
	Modified residue	3	1	N-acetylaspartate By similarity
	Modified residue	55	1	Phosphotyrosine By similarity
	Modified residue	63	1	N6-acetyllysine By similarity
	Modified residue	70	1	N6-acetyllysine By similarity
	Modified residue	75	1	Tele-methylhistidine By similarity
	Modified residue	93	1	Phosphotyrosine By similarity
	Modified residue	193	1	N6-acetyllysine By similarity
	Modified residue	242	1	Phosphotyrosine By similarity
	Modified residue	328	1	N6-acetyllysine By similarity
	Modified residue	330	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68136-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: DF2A3A046346A179	FASTA	377	42,051
	10 20 30 40 50 60 MCDEDETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA 70 80 90 100 110 120 QSKRGILTLK YPIEHGIITN WDDMEKIWHH TFYNELRVAP EEHPTLLTEA PLNPKANREK 130 140 150 160 170 180 MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VTHNVPIYEG YALPHAIMRL 190 200 210 220 230 240 DLAGRDLTDY LMKILTERGY SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK 250 260 270 280 290 300 SYELPDGQVI TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV 310 320 330 340 350 360 MSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS LSTFQQMWIT 370 KQEYDEAGPS IVHRKCF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the rat skeletal muscle actin gene." Zakut R., Shani M., Givol D., Neuman S., Yaffe D., Nudel U. Nature 298:857-859(1982) [PubMed: 6287276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[3]	"Skeletal muscle actin mRNA. Characterization of the 3' untranslated region." Shani M., Nudel U., Zevin-Sonkin D., Zakut R., Givol D., Katcoff D., Carmon Y., Reiter J., Frischauf A.-M., Yaffe D. Nucleic Acids Res. 9:579-589(1981) [PubMed: 6894330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 353-377.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	V01218 Genomic DNA. Translation: CAA24529.1. BC061974 mRNA. Translation: AAH61974.1. V01224 mRNA. Translation: CAA24534.1.
IPI	IPI00189813.
PIR	ATRT. A93283.
RefSeq	NP_062085.1.
UniGene	Rn.82732
3D structure databases
SMR	P68136. Positions 6-373.
ModBase	Search...
Protein-protein interaction databases
STRING	P68136.
PTM databases
PhosphoSite	P68136.
Proteomic databases
PRIDE	P68136.
Genome annotation databases
Ensembl	ENSRNOT00000024084; ENSRNOP00000024084; ENSRNOG00000017786; Rattus norvegicus. [Genome view]
GeneID	29437.
KEGG	rno:29437.
UCSC	NM_019212. rat.
Organism-specific databases
CTD	29437.
RGD	2025. Acta1.
Phylogenomic databases
HOVERGEN	P68136.
OMA	FVGMESA
Gene expression databases
ArrayExpress	P68136.
Genevestigator	P68136.
GermOnline	ENSRNOG00000017786. Rattus norvegicus.
Family and domain databases
InterPro	IPR004000. Actin-like. IPR004001. Actin_CS. [Graphical view]
PANTHER	PTHR11937. Actin_like. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS00406. ACTINS_1. 1 hit. PS00432. ACTINS_2. 1 hit. PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	609178.

Entry information

Entry name

ACTS_RAT

Accession

Primary (citable) accession number: P68136
Secondary accession number(s): P02568, P99020

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 24, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)