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Protein

Elongation factor 1-alpha 1

Gene

EEF1A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTPBy similarity8
Nucleotide bindingi91 – 95GTPBy similarity5
Nucleotide bindingi153 – 156GTPBy similarity4

GO - Molecular functioni

  • calmodulin binding Source: Ensembl
  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • translation elongation factor activity Source: UniProtKB
  • tRNA binding Source: UniProtKB

GO - Biological processi

  • cellular response to epidermal growth factor stimulus Source: UniProtKB
  • neutrophil degranulation Source: Reactome
  • regulation of chaperone-mediated autophagy Source: ParkinsonsUK-UCL
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • translational elongation Source: UniProtKB

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis, Transcription, Transcription regulation
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-156842 Eukaryotic Translation Elongation
R-HSA-156902 Peptide chain elongation
R-HSA-3371511 HSF1 activation
R-HSA-6798695 Neutrophil degranulation
R-HSA-8876725 Protein methylation
SignaLinkiP68104
SIGNORiP68104

Protein family/group databases

MoonProtiP68104

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alpha 1
Short name:
EF-1-alpha-1
Alternative name(s):
Elongation factor Tu
Short name:
EF-Tu
Eukaryotic elongation factor 1 A-1
Short name:
eEF1A-1
Leukocyte receptor cluster member 7
Gene namesi
Name:EEF1A1
Synonyms:EEF1A, EF1A, LENG7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000156508.17
HGNCiHGNC:3189 EEF1A1
MIMi130590 gene
neXtProtiNX_P68104

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36K → R: No effect on methylation by EEF1AKMT2. Abolishes EEF1AKMT4-mediated methylation. 2 Publications1
Mutagenesisi55K → R: No effect on methylation by EEF1AKMT2. 1 Publication1
Mutagenesisi79K → R: No effect on methylation by EEF1AKMT2. 1 Publication1
Mutagenesisi165K → A: Abolishes methylation by EEF1AKMT3. 1 Publication1
Mutagenesisi165K → R: No effect on methylation by EEF1AKMT2. 1 Publication1
Mutagenesisi318K → R: Abolishes methylation by EEF1AKMT2. 1 Publication1
Mutagenesisi432T → A: Abolishes phosphorylation by PASK. 1 Publication1

Organism-specific databases

DisGeNETi1915
OpenTargetsiENSG00000156508
PharmGKBiPA27625

Chemistry databases

ChEMBLiCHEMBL1795120
DrugBankiDB04315 Guanosine-5'-Diphosphate

Polymorphism and mutation databases

BioMutaiEEF1A1
DMDMi55584035

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000908852 – 462Elongation factor 1-alpha 1Add BLAST461

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylglycine1 Publication1
Modified residuei36N6,N6,N6-trimethyllysine; alternate; by EEF1AKMT41 Publication1
Modified residuei36N6,N6-dimethyllysine; alternate; by EEF1AKMT41 Publication1
Modified residuei36N6-methyllysine; alternate; by EEF1AKMT41 Publication1
Modified residuei55N6,N6-dimethyllysine1 Publication1
Modified residuei79N6,N6,N6-trimethyllysine; by EEF1AKMT11 Publication1
Modified residuei165N6,N6,N6-trimethyllysine; alternate; by EEF1AKMT31 Publication1
Modified residuei165N6,N6-dimethyllysine; alternate; by EEF1AKMT32 Publications1
Modified residuei165N6-acetyllysine; alternateBy similarity1
Modified residuei165N6-methyllysine; alternate; by EEF1AKMT31 Publication1
Modified residuei172N6-acetyllysineBy similarity1
Modified residuei273N6-acetyllysineBy similarity1
Modified residuei300Phosphoserine; by TGFBR11 Publication1
Modified residuei3015-glutamyl glycerylphosphorylethanolamine1 Publication1
Modified residuei318N6,N6,N6-trimethyllysine; by EEF1AKMT21 Publication1
Modified residuei3745-glutamyl glycerylphosphorylethanolamine1 Publication1
Modified residuei392N6-acetyllysine; alternateBy similarity1
Modified residuei392N6-succinyllysine; alternateBy similarity1
Modified residuei432Phosphothreonine; by PASK1 Publication1
Modified residuei439N6-acetyllysineBy similarity1

Post-translational modificationi

ISGylated.1 Publication
Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2 (PubMed:26497934).4 Publications
Trimethylated at Lys-79 by EEF1AKMT1 (PubMed:26545399). Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation (PubMed:28108655). Trimethylated at Lys-318 by EEF1AKMT2 (PubMed:25144183). Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs (PubMed:28520920).4 Publications

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP68104
PaxDbiP68104
PeptideAtlasiP68104
PRIDEiP68104
ProteomicsDBi57529

2D gel databases

OGPiP68104
SWISS-2DPAGEiP68104
UCD-2DPAGEiP68104

PTM databases

iPTMnetiP68104
PhosphoSitePlusiP68104
SwissPalmiP68104

Miscellaneous databases

PMAP-CutDBiP68104

Expressioni

Tissue specificityi

Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.

Inductioni

By homocysteine (HC), may mediate accelerated synthesis of free thiol-containing proteins in response to HC-induced oxidative stress.1 Publication

Gene expression databases

BgeeiENSG00000156508
CleanExiHS_EEF1A1
ExpressionAtlasiP68104 baseline and differential
GenevisibleiP68104 HS

Organism-specific databases

HPAiHPA051759
HPA053862
HPA056990

Interactioni

Subunit structurei

Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with PARP1, TXK, XPO5 and KARS. May interact with ERGIC2. Interacts with IFIT1 (via TPR repeats 4-7) (By similarity). Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation. Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner. Interacts with PPP1R16B (PubMed:26497934).By similarity8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • calmodulin binding Source: Ensembl
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108237, 403 interactors
CORUMiP68104
DIPiDIP-31277N
IntActiP68104, 189 interactors
MINTiP68104
STRINGi9606.ENSP00000330054

Chemistry databases

BindingDBiP68104

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SYWmodel-A2-443[»]
3C5JX-ray1.80C343-355[»]
ProteinModelPortaliP68104
SMRiP68104
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 242tr-type GAdd BLAST238

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 21G1By similarity8
Regioni70 – 74G2By similarity5
Regioni91 – 94G3By similarity4
Regioni153 – 156G4By similarity4
Regioni194 – 196G5By similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0052 Eukaryota
COG5256 LUCA
GeneTreeiENSGT00910000144046
HOGENOMiHOG000229291
HOVERGENiHBG000179
InParanoidiP68104
KOiK03231
OMAiPAKETKM
OrthoDBiEOG091G05LW
PhylomeDBiP68104
TreeFamiTF300304

Family and domain databases

HAMAPiMF_00118_A EF_Tu_A, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004539 Transl_elong_EF1A_euk/arc
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00483 EF-1_alpha, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P68104-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV
160 170 180 190 200
GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN
210 220 230 240 250
MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL
260 270 280 290 300
QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS
310 320 330 340 350
EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP
360 370 380 390 400
GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA
410 420 430 440 450
IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK
460
VTKSAQKAQK AK
Length:462
Mass (Da):50,141
Last modified:August 13, 1987 - v1
Checksum:iD465615545AF686A
GO
Isoform 2 (identifier: P68104-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-161: Missing.

Note: No experimental confirmation available.
Show »
Length:441
Mass (Da):47,869
Checksum:i7BF0B64CAEB4B0F6
GO

Sequence cautioni

The sequence AAA52367 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83S → A in CAA27325 (PubMed:3960725).Curated1
Sequence conflicti232L → V in CAA34756 (PubMed:2183196).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057184141 – 161Missing in isoform 2. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03558 mRNA Translation: CAA27245.1
J04617 Genomic DNA Translation: AAA52343.1
X16869 mRNA Translation: CAA34756.1
AY043301 mRNA Translation: AAK95378.1
BC008587 mRNA Translation: AAH08587.1
BC009733 mRNA Translation: AAH09733.1
BC009875 mRNA Translation: AAH09875.1
BC010735 mRNA Translation: AAH10735.1
BC012891 mRNA Translation: AAH12891.1
BC014224 mRNA Translation: AAH14224.1
BC018150 mRNA Translation: AAH18150.1
BC018641 mRNA Translation: AAH18641.1
BC021686 mRNA Translation: AAH21686.1
BC028674 mRNA Translation: AAH28674.1
BC038339 mRNA Translation: AAH38339.1
BC057391 mRNA Translation: AAH57391.1
BC066893 mRNA Translation: AAH66893.1
BC071619 mRNA Translation: AAH71619.1
BC072385 mRNA Translation: AAH72385.1
BC082268 mRNA Translation: AAH82268.1
X03689 mRNA Translation: CAA27325.1
M29548 mRNA Translation: AAA52367.1 Different initiation.
CCDSiCCDS4980.1 [P68104-1]
PIRiB24977 EFHU1
RefSeqiNP_001393.1, NM_001402.5 [P68104-1]
XP_011533816.1, XM_011535514.2 [P68104-1]
UniGeneiHs.535192
Hs.586423
Hs.745122

Genome annotation databases

EnsembliENST00000309268; ENSP00000339053; ENSG00000156508 [P68104-1]
ENST00000316292; ENSP00000339063; ENSG00000156508 [P68104-1]
ENST00000331523; ENSP00000330054; ENSG00000156508 [P68104-1]
GeneIDi1915
KEGGihsa:1915

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEF1A1_HUMAN
AccessioniPrimary (citable) accession number: P68104
Secondary accession number(s): P04719, P04720, Q6IQ15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: June 20, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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