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Protein

Elongation factor 1-alpha 1

Gene

EEF1A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTPBy similarity8
Nucleotide bindingi91 – 95GTPBy similarity5
Nucleotide bindingi153 – 156GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • translation elongation factor activity Source: UniProtKB
  • tRNA binding Source: UniProtKB

GO - Biological processi

  • cellular response to epidermal growth factor stimulus Source: UniProtKB
  • neutrophil degranulation Source: Reactome
  • protein methylation Source: Reactome
  • regulation of chaperone-mediated autophagy Source: ParkinsonsUK-UCL
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • translational elongation Source: UniProtKB

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis, Transcription, Transcription regulation
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-156842. Eukaryotic Translation Elongation.
R-HSA-156902. Peptide chain elongation.
R-HSA-3371511. HSF1 activation.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-8876725. Protein methylation.
SignaLinkiP68104.
SIGNORiP68104.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alpha 1
Short name:
EF-1-alpha-1
Alternative name(s):
Elongation factor Tu
Short name:
EF-Tu
Eukaryotic elongation factor 1 A-1
Short name:
eEF1A-1
Leukocyte receptor cluster member 7
Gene namesi
Name:EEF1A1
Synonyms:EEF1A, EF1A, LENG7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000156508.17.
HGNCiHGNC:3189. EEF1A1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36K → R: No effect on methylation by EEF1AKMT2. 1 Publication1
Mutagenesisi55K → R: No effect on methylation by EEF1AKMT2. 1 Publication1
Mutagenesisi79K → R: No effect on methylation by EEF1AKMT2. 1 Publication1
Mutagenesisi165K → A: Abolishes methylation by EEF1AKMT3. 1 Publication1
Mutagenesisi165K → R: No effect on methylation by EEF1AKMT2. 1 Publication1
Mutagenesisi318K → R: Abolishes methylation by EEF1AKMT2. 1 Publication1
Mutagenesisi432T → A: Abolishes phosphorylation by PASK. 1 Publication1

Organism-specific databases

DisGeNETi1915.
OpenTargetsiENSG00000156508.
PharmGKBiPA27625.

Chemistry databases

ChEMBLiCHEMBL1795120.
DrugBankiDB04315. Guanosine-5'-Diphosphate.

Polymorphism and mutation databases

BioMutaiEEF1A1.
DMDMi55584035.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000908852 – 462Elongation factor 1-alpha 1Add BLAST461

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylglycine1 Publication1
Modified residuei36N6,N6,N6-trimethyllysineBy similarity1
Modified residuei55N6,N6-dimethyllysine1 Publication1
Modified residuei79N6,N6,N6-trimethyllysine; by EEF1AKMT11 Publication1
Modified residuei165N6,N6,N6-trimethyllysine; alternate; by EEF1AKMT31 Publication1
Modified residuei165N6,N6-dimethyllysine; alternate; by EEF1AKMT32 Publications1
Modified residuei165N6-acetyllysine; alternateBy similarity1
Modified residuei165N6-methyllysine; alternate; by EEF1AKMT31 Publication1
Modified residuei172N6-acetyllysineBy similarity1
Modified residuei224PhosphoserineBy similarity1
Modified residuei273N6-acetyllysineBy similarity1
Modified residuei300Phosphoserine; by TGFBR11 Publication1
Modified residuei3015-glutamyl glycerylphosphorylethanolamine1 Publication1
Modified residuei318N6,N6,N6-trimethyllysine; by EEF1AKMT21 Publication1
Modified residuei3745-glutamyl glycerylphosphorylethanolamine1 Publication1
Modified residuei392N6-acetyllysine; alternateBy similarity1
Modified residuei392N6-succinyllysine; alternateBy similarity1
Modified residuei432Phosphothreonine; by PASK1 Publication1
Modified residuei439N6-acetyllysineBy similarity1

Post-translational modificationi

ISGylated.1 Publication
Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2 (PubMed:26497934).4 Publications
Trimethylated at Lys-79 by EEF1AKMT1 (PubMed:26545399). Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation (PubMed:28108655). Trimethylated at Lys-318 by EEF1AKMT2 (PubMed:25144183).3 Publications

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP68104.
PaxDbiP68104.
PeptideAtlasiP68104.
PRIDEiP68104.

2D gel databases

OGPiP68104.
SWISS-2DPAGEiP68104.
UCD-2DPAGEiP68104.

PTM databases

iPTMnetiP68104.
PhosphoSitePlusiP68104.
SwissPalmiP68104.

Miscellaneous databases

PMAP-CutDBiP68104.

Expressioni

Tissue specificityi

Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.

Inductioni

By homocysteine (HC), may mediate accelerated synthesis of free thiol-containing proteins in response to HC-induced oxidative stress.1 Publication

Gene expression databases

BgeeiENSG00000156508.
CleanExiHS_EEF1A1.
ExpressionAtlasiP68104. baseline and differential.
GenevisibleiP68104. HS.

Organism-specific databases

HPAiHPA051759.
HPA053862.
HPA056990.

Interactioni

Subunit structurei

Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with PARP1, TXK, XPO5 and KARS. May interact with ERGIC2. Interacts with IFIT1 (via TPR repeats 4-7) (By similarity). Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation. Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner. Interacts with PPP1R16B (PubMed:26497934).By similarity8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108237. 401 interactors.
CORUMiP68104.
DIPiDIP-31277N.
IntActiP68104. 187 interactors.
MINTiMINT-1180846.
STRINGi9606.ENSP00000330054.

Chemistry databases

BindingDBiP68104.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SYWmodel-A2-443[»]
3C5JX-ray1.80C343-355[»]
ProteinModelPortaliP68104.
SMRiP68104.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 242tr-type GAdd BLAST238

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 21G1By similarity8
Regioni70 – 74G2By similarity5
Regioni91 – 94G3By similarity4
Regioni153 – 156G4By similarity4
Regioni194 – 196G5By similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0052. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00670000097815.
HOGENOMiHOG000229291.
HOVERGENiHBG000179.
InParanoidiP68104.
KOiK03231.
OMAiPAKETKM.
OrthoDBiEOG091G05LW.
PhylomeDBiP68104.
TreeFamiTF300304.

Family and domain databases

HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiView protein in InterPro
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel_sf.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
PfamiView protein in Pfam
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiView protein in PROSITE
PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P68104-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV
160 170 180 190 200
GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN
210 220 230 240 250
MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL
260 270 280 290 300
QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS
310 320 330 340 350
EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP
360 370 380 390 400
GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA
410 420 430 440 450
IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK
460
VTKSAQKAQK AK
Length:462
Mass (Da):50,141
Last modified:August 13, 1987 - v1
Checksum:iD465615545AF686A
GO
Isoform 2 (identifier: P68104-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-161: Missing.

Note: No experimental confirmation available.
Show »
Length:441
Mass (Da):47,869
Checksum:i7BF0B64CAEB4B0F6
GO

Sequence cautioni

The sequence AAA52367 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83S → A in CAA27325 (PubMed:3960725).Curated1
Sequence conflicti232L → V in CAA34756 (PubMed:2183196).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057184141 – 161Missing in isoform 2. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03558 mRNA. Translation: CAA27245.1.
J04617 Genomic DNA. Translation: AAA52343.1.
X16869 mRNA. Translation: CAA34756.1.
AY043301 mRNA. Translation: AAK95378.1.
BC008587 mRNA. Translation: AAH08587.1.
BC009733 mRNA. Translation: AAH09733.1.
BC009875 mRNA. Translation: AAH09875.1.
BC010735 mRNA. Translation: AAH10735.1.
BC012891 mRNA. Translation: AAH12891.1.
BC014224 mRNA. Translation: AAH14224.1.
BC018150 mRNA. Translation: AAH18150.1.
BC018641 mRNA. Translation: AAH18641.1.
BC021686 mRNA. Translation: AAH21686.1.
BC028674 mRNA. Translation: AAH28674.1.
BC038339 mRNA. Translation: AAH38339.1.
BC057391 mRNA. Translation: AAH57391.1.
BC066893 mRNA. Translation: AAH66893.1.
BC071619 mRNA. Translation: AAH71619.1.
BC072385 mRNA. Translation: AAH72385.1.
BC082268 mRNA. Translation: AAH82268.1.
X03689 mRNA. Translation: CAA27325.1.
M29548 mRNA. Translation: AAA52367.1. Different initiation.
CCDSiCCDS4980.1. [P68104-1]
PIRiB24977. EFHU1.
RefSeqiNP_001393.1. NM_001402.5. [P68104-1]
XP_011533816.1. XM_011535514.2. [P68104-1]
UniGeneiHs.535192.
Hs.586423.
Hs.745122.

Genome annotation databases

EnsembliENST00000309268; ENSP00000339053; ENSG00000156508. [P68104-1]
ENST00000316292; ENSP00000339063; ENSG00000156508. [P68104-1]
ENST00000331523; ENSP00000330054; ENSG00000156508. [P68104-1]
GeneIDi1915.
KEGGihsa:1915.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEF1A1_HUMAN
AccessioniPrimary (citable) accession number: P68104
Secondary accession number(s): P04719, P04720, Q6IQ15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 22, 2017
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families