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Protein

Elongation factor 1-alpha 1

Gene

EEF1A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218GTPBy similarity
Nucleotide bindingi91 – 955GTPBy similarity
Nucleotide bindingi153 – 1564GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • translation elongation factor activity Source: UniProtKB
  • tRNA binding Source: UniProtKB

GO - Biological processi

  • cellular response to epidermal growth factor stimulus Source: UniProtKB
  • regulation of chaperone-mediated autophagy Source: ParkinsonsUK-UCL
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • translational elongation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis, Transcription, Transcription regulation

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-156842. Eukaryotic Translation Elongation.
R-HSA-156902. Peptide chain elongation.
R-HSA-3371511. HSF1 activation.
SignaLinkiP68104.
SIGNORiP68104.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alpha 1
Short name:
EF-1-alpha-1
Alternative name(s):
Elongation factor Tu
Short name:
EF-Tu
Eukaryotic elongation factor 1 A-1
Short name:
eEF1A-1
Leukocyte receptor cluster member 7
Gene namesi
Name:EEF1A1
Synonyms:EEF1A, EF1A, LENG7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:3189. EEF1A1.

Subcellular locationi

  • Cytoplasm
  • Nucleus
  • Nucleusnucleolus

  • Note: Colocalizes with DLC1 at actin-rich regions in the cell periphery. Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens.

GO - Cellular componenti

  • cortical actin cytoskeleton Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytoplasmic side of lysosomal membrane Source: ParkinsonsUK-UCL
  • cytosol Source: UniProtKB
  • eukaryotic translation elongation factor 1 complex Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • membrane Source: UniProtKB
  • nucleolus Source: UniProtKB
  • nucleus Source: UniProtKB
  • ruffle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi432 – 4321T → A: Abolishes phosphorylation by PASK. 1 Publication

Organism-specific databases

PharmGKBiPA27625.

Chemistry

ChEMBLiCHEMBL1795120.

Polymorphism and mutation databases

BioMutaiEEF1A1.
DMDMi55584035.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Elongation factor 1-alpha 1PRO_0000090885Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361N6,N6,N6-trimethyllysineBy similarity
Modified residuei55 – 551N6,N6-dimethyllysine1 Publication
Modified residuei79 – 791N6,N6,N6-trimethyllysineBy similarity
Modified residuei165 – 1651N6,N6-dimethyllysine; alternate1 Publication
Modified residuei165 – 1651N6-acetyllysine; alternateBy similarity
Modified residuei172 – 1721N6-acetyllysineBy similarity
Modified residuei273 – 2731N6-acetyllysineBy similarity
Modified residuei300 – 3001Phosphoserine; by TGFBR11 Publication
Modified residuei301 – 30115-glutamyl glycerylphosphorylethanolamine1 Publication
Modified residuei318 – 3181N6,N6,N6-trimethyllysineBy similarity
Modified residuei374 – 37415-glutamyl glycerylphosphorylethanolamine1 Publication
Modified residuei392 – 3921N6-acetyllysine; alternateBy similarity
Modified residuei392 – 3921N6-succinyllysine; alternateBy similarity
Modified residuei432 – 4321Phosphothreonine; by PASK1 Publication
Modified residuei439 – 4391N6-acetyllysineBy similarity

Post-translational modificationi

ISGylated.1 Publication
Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency.3 Publications

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP68104.
PaxDbiP68104.
PeptideAtlasiP68104.
PRIDEiP68104.

2D gel databases

OGPiP68104.
SWISS-2DPAGEP68104.
UCD-2DPAGEP68104.

PTM databases

iPTMnetiP68104.
PhosphoSiteiP68104.
SwissPalmiP68104.

Miscellaneous databases

PMAP-CutDBP68104.

Expressioni

Tissue specificityi

Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.

Inductioni

By homocysteine (HC), may mediate accelerated synthesis of free thiol-containing proteins in response to HC-induced oxidative stress.1 Publication

Gene expression databases

BgeeiENSG00000156508.
CleanExiHS_EEF1A1.
ExpressionAtlasiP68104. baseline and differential.
GenevisibleiP68104. HS.

Organism-specific databases

HPAiHPA051759.
HPA053862.
HPA056990.

Interactioni

Subunit structurei

Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with PARP1, TXK, XPO5 and KARS. May interact with ERGIC2. Interacts with IFIT1 (via TPR repeats 4-7) (By similarity). Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation. Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner.By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BBS1Q8NFJ93EBI-352162,EBI-1805484
MDM2Q009879EBI-352162,EBI-389668
RAD23AP547252EBI-352162,EBI-746453
SPHK1Q9NYA12EBI-352162,EBI-985303
YWHAZP631042EBI-352162,EBI-347088
ZBTB16Q055164EBI-352162,EBI-711925
ZDHHC17Q8IUH52EBI-352162,EBI-524753

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108237. 385 interactions.
DIPiDIP-31277N.
IntActiP68104. 181 interactions.
MINTiMINT-1180846.
STRINGi9606.ENSP00000330054.

Chemistry

BindingDBiP68104.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SYWmodel-A2-443[»]
3C5JX-ray1.80C343-355[»]
ProteinModelPortaliP68104.
SMRiP68104. Positions 4-455.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 242238tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 218G1By similarity
Regioni70 – 745G2By similarity
Regioni91 – 944G3By similarity
Regioni153 – 1564G4By similarity
Regioni194 – 1963G5By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0052. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00670000097815.
HOGENOMiHOG000229291.
HOVERGENiHBG000179.
InParanoidiP68104.
KOiK03231.
OMAiFEQISHE.
OrthoDBiEOG091G05LW.
PhylomeDBiP68104.
TreeFamiTF300304.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P68104-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV
160 170 180 190 200
GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN
210 220 230 240 250
MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL
260 270 280 290 300
QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS
310 320 330 340 350
EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP
360 370 380 390 400
GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA
410 420 430 440 450
IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK
460
VTKSAQKAQK AK
Length:462
Mass (Da):50,141
Last modified:August 13, 1987 - v1
Checksum:iD465615545AF686A
GO
Isoform 2 (identifier: P68104-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-161: Missing.

Note: No experimental confirmation available.
Show »
Length:441
Mass (Da):47,869
Checksum:i7BF0B64CAEB4B0F6
GO

Sequence cautioni

The sequence AAA52367 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831S → A in CAA27325 (PubMed:3960725).Curated
Sequence conflicti232 – 2321L → V in CAA34756 (PubMed:2183196).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei141 – 16121Missing in isoform 2. 1 PublicationVSP_057184Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03558 mRNA. Translation: CAA27245.1.
J04617 Genomic DNA. Translation: AAA52343.1.
X16869 mRNA. Translation: CAA34756.1.
AY043301 mRNA. Translation: AAK95378.1.
BC008587 mRNA. Translation: AAH08587.1.
BC009733 mRNA. Translation: AAH09733.1.
BC009875 mRNA. Translation: AAH09875.1.
BC010735 mRNA. Translation: AAH10735.1.
BC012891 mRNA. Translation: AAH12891.1.
BC014224 mRNA. Translation: AAH14224.1.
BC018150 mRNA. Translation: AAH18150.1.
BC018641 mRNA. Translation: AAH18641.1.
BC021686 mRNA. Translation: AAH21686.1.
BC028674 mRNA. Translation: AAH28674.1.
BC038339 mRNA. Translation: AAH38339.1.
BC057391 mRNA. Translation: AAH57391.1.
BC066893 mRNA. Translation: AAH66893.1.
BC071619 mRNA. Translation: AAH71619.1.
BC072385 mRNA. Translation: AAH72385.1.
BC082268 mRNA. Translation: AAH82268.1.
X03689 mRNA. Translation: CAA27325.1.
M29548 mRNA. Translation: AAA52367.1. Different initiation.
CCDSiCCDS4980.1. [P68104-1]
PIRiB24977. EFHU1.
RefSeqiNP_001393.1. NM_001402.5. [P68104-1]
XP_011533816.1. XM_011535514.2. [P68104-1]
UniGeneiHs.535192.
Hs.586423.
Hs.745122.

Genome annotation databases

EnsembliENST00000309268; ENSP00000339053; ENSG00000156508. [P68104-1]
ENST00000316292; ENSP00000339063; ENSG00000156508. [P68104-1]
ENST00000331523; ENSP00000330054; ENSG00000156508. [P68104-1]
GeneIDi1915.
KEGGihsa:1915.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03558 mRNA. Translation: CAA27245.1.
J04617 Genomic DNA. Translation: AAA52343.1.
X16869 mRNA. Translation: CAA34756.1.
AY043301 mRNA. Translation: AAK95378.1.
BC008587 mRNA. Translation: AAH08587.1.
BC009733 mRNA. Translation: AAH09733.1.
BC009875 mRNA. Translation: AAH09875.1.
BC010735 mRNA. Translation: AAH10735.1.
BC012891 mRNA. Translation: AAH12891.1.
BC014224 mRNA. Translation: AAH14224.1.
BC018150 mRNA. Translation: AAH18150.1.
BC018641 mRNA. Translation: AAH18641.1.
BC021686 mRNA. Translation: AAH21686.1.
BC028674 mRNA. Translation: AAH28674.1.
BC038339 mRNA. Translation: AAH38339.1.
BC057391 mRNA. Translation: AAH57391.1.
BC066893 mRNA. Translation: AAH66893.1.
BC071619 mRNA. Translation: AAH71619.1.
BC072385 mRNA. Translation: AAH72385.1.
BC082268 mRNA. Translation: AAH82268.1.
X03689 mRNA. Translation: CAA27325.1.
M29548 mRNA. Translation: AAA52367.1. Different initiation.
CCDSiCCDS4980.1. [P68104-1]
PIRiB24977. EFHU1.
RefSeqiNP_001393.1. NM_001402.5. [P68104-1]
XP_011533816.1. XM_011535514.2. [P68104-1]
UniGeneiHs.535192.
Hs.586423.
Hs.745122.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SYWmodel-A2-443[»]
3C5JX-ray1.80C343-355[»]
ProteinModelPortaliP68104.
SMRiP68104. Positions 4-455.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108237. 385 interactions.
DIPiDIP-31277N.
IntActiP68104. 181 interactions.
MINTiMINT-1180846.
STRINGi9606.ENSP00000330054.

Chemistry

BindingDBiP68104.
ChEMBLiCHEMBL1795120.

PTM databases

iPTMnetiP68104.
PhosphoSiteiP68104.
SwissPalmiP68104.

Polymorphism and mutation databases

BioMutaiEEF1A1.
DMDMi55584035.

2D gel databases

OGPiP68104.
SWISS-2DPAGEP68104.
UCD-2DPAGEP68104.

Proteomic databases

EPDiP68104.
PaxDbiP68104.
PeptideAtlasiP68104.
PRIDEiP68104.

Protocols and materials databases

DNASUi1915.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309268; ENSP00000339053; ENSG00000156508. [P68104-1]
ENST00000316292; ENSP00000339063; ENSG00000156508. [P68104-1]
ENST00000331523; ENSP00000330054; ENSG00000156508. [P68104-1]
GeneIDi1915.
KEGGihsa:1915.

Organism-specific databases

CTDi1915.
GeneCardsiEEF1A1.
H-InvDBHIX0020005.
HIX0032108.
HIX0033669.
HIX0169110.
HGNCiHGNC:3189. EEF1A1.
HPAiHPA051759.
HPA053862.
HPA056990.
MIMi130590. gene.
neXtProtiNX_P68104.
PharmGKBiPA27625.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0052. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00670000097815.
HOGENOMiHOG000229291.
HOVERGENiHBG000179.
InParanoidiP68104.
KOiK03231.
OMAiFEQISHE.
OrthoDBiEOG091G05LW.
PhylomeDBiP68104.
TreeFamiTF300304.

Enzyme and pathway databases

ReactomeiR-HSA-156842. Eukaryotic Translation Elongation.
R-HSA-156902. Peptide chain elongation.
R-HSA-3371511. HSF1 activation.
SignaLinkiP68104.
SIGNORiP68104.

Miscellaneous databases

ChiTaRSiEEF1A1. human.
GeneWikiiEukaryotic_translation_elongation_factor_1_alpha_1.
GenomeRNAii1915.
PMAP-CutDBP68104.
PROiP68104.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000156508.
CleanExiHS_EEF1A1.
ExpressionAtlasiP68104. baseline and differential.
GenevisibleiP68104. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF1A1_HUMAN
AccessioniPrimary (citable) accession number: P68104
Secondary accession number(s): P04719, P04720, Q6IQ15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 7, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.