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Protein

Autonomous glycyl radical cofactor

Gene

grcA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a radical domain for damaged PFL and possibly other radical proteins.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:EG11784-MONOMER.
ECOL316407:JW2563-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autonomous glycyl radical cofactor
Gene namesi
Name:grcA
Synonyms:yfiD
Ordered Locus Names:b2579, JW2563
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11784. grcA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells undergo an apoptotic-like death upon DNA damage characterized by membrane depolarization; further disruption of recA prevents membrane depolarization.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 127127Autonomous glycyl radical cofactorPRO_0000166697Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481N6-acetyllysine1 Publication
Modified residuei88 – 881N6-acetyllysine1 Publication
Modified residuei92 – 921N6-acetyllysine1 Publication
Modified residuei102 – 1021Glycine radicalBy similarity

Keywords - PTMi

Acetylation, Organic radical

Proteomic databases

EPDiP68066.
PaxDbiP68066.
PRIDEiP68066.

2D gel databases

SWISS-2DPAGEP68066.

Expressioni

Inductioni

By acid stress.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263475. 6 interactions.
DIPiDIP-47913N.
IntActiP68066. 12 interactions.
STRINGi511145.b2579.

Structurei

3D structure databases

ProteinModelPortaliP68066.
SMRiP68066. Positions 13-127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 127123Glycine radicalAdd
BLAST

Sequence similaritiesi

Contains 1 glycine radical domain.Curated

Phylogenomic databases

eggNOGiENOG4108VKA. Bacteria.
COG3445. LUCA.
HOGENOMiHOG000281256.
KOiK06866.
OMAiGQFEYRE.
OrthoDBiEOG62C9MX.

Family and domain databases

HAMAPiMF_00806. GrcA.
InterProiIPR019777. Form_AcTrfase_GR_CS.
IPR001150. Gly_radical.
IPR011140. Glycyl_radical_cofactor_GrcA.
[Graphical view]
PfamiPF01228. Gly_radical. 1 hit.
[Graphical view]
PIRSFiPIRSF000378. Gly_radicl_yfiD. 1 hit.
TIGRFAMsiTIGR04365. spare_glycyl. 1 hit.
PROSITEiPS00850. GLY_RADICAL_1. 1 hit.
PS51149. GLY_RADICAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITGIQITKA ANDDLLNSFW LLDSEKGEAR CIVAKAGYAE DEVVAVSKLG
60 70 80 90 100
DIEYREVPVE VKPEVRVEGG QHLNVNVLRR ETLEDAVKHP EKYPQLTIRV
110 120
SGYAVRFNSL TPEQQRDVIA RTFTESL
Length:127
Mass (Da):14,284
Last modified:December 21, 2004 - v1
Checksum:i6BA617E67D6540CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13169 Genomic DNA. No translation available.
D64044 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75632.1.
AP009048 Genomic DNA. Translation: BAA16465.1.
J03725 Genomic DNA. No translation available.
PIRiB65036.
RefSeqiNP_417074.1. NC_000913.3.
WP_000627807.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75632; AAC75632; b2579.
BAA16465; BAA16465; BAA16465.
GeneIDi947068.
KEGGiecj:JW2563.
eco:b2579.
PATRICi32120559. VBIEscCol129921_2682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13169 Genomic DNA. No translation available.
D64044 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75632.1.
AP009048 Genomic DNA. Translation: BAA16465.1.
J03725 Genomic DNA. No translation available.
PIRiB65036.
RefSeqiNP_417074.1. NC_000913.3.
WP_000627807.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP68066.
SMRiP68066. Positions 13-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263475. 6 interactions.
DIPiDIP-47913N.
IntActiP68066. 12 interactions.
STRINGi511145.b2579.

2D gel databases

SWISS-2DPAGEP68066.

Proteomic databases

EPDiP68066.
PaxDbiP68066.
PRIDEiP68066.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75632; AAC75632; b2579.
BAA16465; BAA16465; BAA16465.
GeneIDi947068.
KEGGiecj:JW2563.
eco:b2579.
PATRICi32120559. VBIEscCol129921_2682.

Organism-specific databases

EchoBASEiEB1732.
EcoGeneiEG11784. grcA.

Phylogenomic databases

eggNOGiENOG4108VKA. Bacteria.
COG3445. LUCA.
HOGENOMiHOG000281256.
KOiK06866.
OMAiGQFEYRE.
OrthoDBiEOG62C9MX.

Enzyme and pathway databases

BioCyciEcoCyc:EG11784-MONOMER.
ECOL316407:JW2563-MONOMER.

Miscellaneous databases

PROiP68066.

Family and domain databases

HAMAPiMF_00806. GrcA.
InterProiIPR019777. Form_AcTrfase_GR_CS.
IPR001150. Gly_radical.
IPR011140. Glycyl_radical_cofactor_GrcA.
[Graphical view]
PfamiPF01228. Gly_radical. 1 hit.
[Graphical view]
PIRSFiPIRSF000378. Gly_radicl_yfiD. 1 hit.
TIGRFAMsiTIGR04365. spare_glycyl. 1 hit.
PROSITEiPS00850. GLY_RADICAL_1. 1 hit.
PS51149. GLY_RADICAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Non-ribosomal proteins affecting the assembly of ribosomes in Escherichia coli."
    Nashimoto H.
    (In) Nierhaus K.H. (eds.); The translational apparatus, pp.185-195, Plenum Press, New York (1993)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Nashimoto H., Saito N.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Sequence analysis, expression, and conservation of Escherichia coli uracil DNA glycosylase and its gene (ung)."
    Varshney U., Hutcheon T., de Sande J.H.
    J. Biol. Chem. 263:7776-7784(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-76.
  7. "Small genes/gene-products in Escherichia coli K-12."
    Wasinger V.C., Humphery-Smith I.
    FEMS Microbiol. Lett. 169:375-382(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10.
    Strain: K12.
  8. Cited for: PROTEIN SEQUENCE OF 1-4.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  9. "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
    Borodovsky M., Rudd K.E., Koonin E.V.
    Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  10. "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
    Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
    Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: B / BL21.
  11. "Expression of the Escherichia coli yfiD gene responds to intracellular pH and reduces the accumulation of acidic metabolic end products."
    Wyborn N.R., Messenger S.L., Henderson R.A., Sawers G., Roberts R.E., Attwood M.M., Green J.
    Microbiology 148:1015-1026(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  12. "YfiD of Escherichia coli and Y06I of bacteriophage T4 as autonomous glycyl radical cofactors reconstituting the catalytic center of oxygen-fragmented pyruvate formate-lyase."
    Wagner A.F.V., Schultz S., Bomke J., Pils T., Lehmann W.D., Knappe J.
    Biochem. Biophys. Res. Commun. 285:456-462(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
    Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
    Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-48; LYS-88 AND LYS-92, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.
  14. "Two programmed cell death systems in Escherichia coli: an apoptotic-like death is inhibited by the mazEF-mediated death pathway."
    Erental A., Sharon I., Engelberg-Kulka H.
    PLoS Biol. 10:E1001281-E1001281(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiGRCA_ECOLI
AccessioniPrimary (citable) accession number: P68066
Secondary accession number(s): P33633
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: March 16, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.