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Protein

Autonomous glycyl radical cofactor

Gene

grcA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a radical domain for damaged PFL and possibly other radical proteins.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:EG11784-MONOMER.
ECOL316407:JW2563-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autonomous glycyl radical cofactor
Gene namesi
Name:grcA
Synonyms:yfiD
Ordered Locus Names:b2579, JW2563
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11784. grcA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells undergo an apoptotic-like death upon DNA damage characterized by membrane depolarization; further disruption of recA prevents membrane depolarization.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001666971 – 127Autonomous glycyl radical cofactorAdd BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48N6-acetyllysine1 Publication1
Modified residuei88N6-acetyllysine1 Publication1
Modified residuei92N6-acetyllysine1 Publication1
Modified residuei102Glycine radicalBy similarity1

Keywords - PTMi

Acetylation, Organic radical

Proteomic databases

EPDiP68066.
PaxDbiP68066.
PRIDEiP68066.

2D gel databases

SWISS-2DPAGEP68066.

Expressioni

Inductioni

By acid stress.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263475. 6 interactors.
DIPiDIP-47913N.
IntActiP68066. 12 interactors.
STRINGi511145.b2579.

Structurei

3D structure databases

ProteinModelPortaliP68066.
SMRiP68066.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 127Glycine radicalAdd BLAST123

Sequence similaritiesi

Contains 1 glycine radical domain.Curated

Phylogenomic databases

eggNOGiENOG4108VKA. Bacteria.
COG3445. LUCA.
HOGENOMiHOG000281256.
KOiK06866.
OMAiGQFEYRE.

Family and domain databases

HAMAPiMF_00806. GrcA. 1 hit.
InterProiIPR019777. Form_AcTrfase_GR_CS.
IPR001150. Gly_radical.
IPR011140. Glycyl_radical_cofactor_GrcA.
[Graphical view]
PfamiPF01228. Gly_radical. 1 hit.
[Graphical view]
PIRSFiPIRSF000378. Gly_radicl_yfiD. 1 hit.
TIGRFAMsiTIGR04365. spare_glycyl. 1 hit.
PROSITEiPS00850. GLY_RADICAL_1. 1 hit.
PS51149. GLY_RADICAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITGIQITKA ANDDLLNSFW LLDSEKGEAR CIVAKAGYAE DEVVAVSKLG
60 70 80 90 100
DIEYREVPVE VKPEVRVEGG QHLNVNVLRR ETLEDAVKHP EKYPQLTIRV
110 120
SGYAVRFNSL TPEQQRDVIA RTFTESL
Length:127
Mass (Da):14,284
Last modified:December 21, 2004 - v1
Checksum:i6BA617E67D6540CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13169 Genomic DNA. No translation available.
D64044 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75632.1.
AP009048 Genomic DNA. Translation: BAA16465.1.
J03725 Genomic DNA. No translation available.
PIRiB65036.
RefSeqiNP_417074.1. NC_000913.3.
WP_000627807.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75632; AAC75632; b2579.
BAA16465; BAA16465; BAA16465.
GeneIDi947068.
KEGGiecj:JW2563.
eco:b2579.
PATRICi32120559. VBIEscCol129921_2682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13169 Genomic DNA. No translation available.
D64044 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75632.1.
AP009048 Genomic DNA. Translation: BAA16465.1.
J03725 Genomic DNA. No translation available.
PIRiB65036.
RefSeqiNP_417074.1. NC_000913.3.
WP_000627807.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP68066.
SMRiP68066.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263475. 6 interactors.
DIPiDIP-47913N.
IntActiP68066. 12 interactors.
STRINGi511145.b2579.

2D gel databases

SWISS-2DPAGEP68066.

Proteomic databases

EPDiP68066.
PaxDbiP68066.
PRIDEiP68066.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75632; AAC75632; b2579.
BAA16465; BAA16465; BAA16465.
GeneIDi947068.
KEGGiecj:JW2563.
eco:b2579.
PATRICi32120559. VBIEscCol129921_2682.

Organism-specific databases

EchoBASEiEB1732.
EcoGeneiEG11784. grcA.

Phylogenomic databases

eggNOGiENOG4108VKA. Bacteria.
COG3445. LUCA.
HOGENOMiHOG000281256.
KOiK06866.
OMAiGQFEYRE.

Enzyme and pathway databases

BioCyciEcoCyc:EG11784-MONOMER.
ECOL316407:JW2563-MONOMER.

Miscellaneous databases

PROiP68066.

Family and domain databases

HAMAPiMF_00806. GrcA. 1 hit.
InterProiIPR019777. Form_AcTrfase_GR_CS.
IPR001150. Gly_radical.
IPR011140. Glycyl_radical_cofactor_GrcA.
[Graphical view]
PfamiPF01228. Gly_radical. 1 hit.
[Graphical view]
PIRSFiPIRSF000378. Gly_radicl_yfiD. 1 hit.
TIGRFAMsiTIGR04365. spare_glycyl. 1 hit.
PROSITEiPS00850. GLY_RADICAL_1. 1 hit.
PS51149. GLY_RADICAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRCA_ECOLI
AccessioniPrimary (citable) accession number: P68066
Secondary accession number(s): P33633
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.