Reviewed, UniProtKB/Swiss-Prot P68040 (GBLP_MOUSE)

Names and origin

Protein names

Recommended name:
    Guanine nucleotide-binding protein subunit beta-2-like 1
Alternative name(s):
    Receptor of activated protein kinase C 1
      Short name=RACK1
    Receptor for activated C kinase
    p205
    12-3

Gene names

Name:	Gnb2l1
Synonyms:	Gnb2-rs1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation By similarity. May play an important role in the developing brain and neuronal differentiation.
Subunit structure	Binds SLC9A3R1. Forms a ternary complex with TRIM63 and PRKCE. Interacts with HABP4 and KRT1 By similarity.
Subcellular location	Cell membrane By similarity.
Tissue specificity	Strongly and ubiquitously expressed in the embryonic and early postnatal brain. This expression decreases during postnatal development and is localized in the dentate gyrus, habenula, piriform cortex, paraventricular nucleus of the hypothalamus and supraoptic nucleus of the adult brain. Ref.1
Domain	The WD repeats domain 5 mediates interaction with TRIM63 By similarity.
Sequence similarities	Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.
Sequence caution	The sequence AAG29506.1 differs from that shown. Reason: Frameshift at positions 232 and 239.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat WD repeat
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cell soma Inferred from direct assay. Source: MGI cytoplasm Inferred from direct assay. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ACHE	P22303	1	EBI-296749,EBI-1637793	From a different organism.
Ikbkb	O88351	3	EBI-296749,EBI-447960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.8
	Chain	2 – 317	316	Guanine nucleotide-binding protein subunit beta-2-like 1		PRO_0000127732
Regions
	Repeat	13 – 44	32	WD 1
	Repeat	61 – 91	31	WD 2
	Repeat	103 – 133	31	WD 3
	Repeat	146 – 178	33	WD 4
	Repeat	190 – 220	31	WD 5
	Repeat	231 – 260	30	WD 6
	Repeat	281 – 311	31	WD 7
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine Ref.8
	Modified residue	130	1	N6-acetyllysine By similarity
	Modified residue	228	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	9	1	G → R in BAE40068. Ref.3
	Sequence conflict	9	1	G → R in BAE40156. Ref.3
	Sequence conflict	125	1	R → Q in BAE40059. Ref.3
	Sequence conflict	125	1	R → Q in BAE40068. Ref.3
	Sequence conflict	125	1	R → Q in BAE40156. Ref.3
	Sequence conflict	248	1	L → P in BAE35378. Ref.3
	Sequence conflict	266	1	I → M in AAG29506. Ref.6
	Sequence conflict	273	1	E → A in BAA06185. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P68040-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 257F91E369ED2044	FASTA	317	35,077
	10 20 30 40 50 60 MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR 70 80 90 100 110 120 GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI 130 140 150 160 170 180 VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA 190 200 210 220 230 240 NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC 250 260 270 280 290 300 FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA 310 GYTDNLVRVW QVTIGTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a neural differentiation-associated gene, p205, in the embryonal carcinoma cell line P19 and in the developing mouse." Imai Y., Suzuki Y., Tohyama M., Wanaka A., Takagi T. Brain Res. Mol. Brain Res. 24:313-319(1994) [PubMed: 7968370] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"Primary sequence of the mouse guanine nucleotide binding protein related gene." Raj N.B.K., Su Y., Au W.C., Pitha P.M. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Spleen.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: BALB/c, C57BL/6J and DBA/2. Tissue: Blastocyst, Embryo, Embryonic head, Embryonic spinal ganglion, Embryonic stem cell, Head, Kidney, Mammary gland and Osteoclast.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[7]	"Genomic structure and expression of mouse receptor for activated C kinase (RACK1)." Choi D., Messing R.O. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-296. Strain: 129/SvJ.
[8]	Kanor S., Quadroni M., Bienvenut W.V. Submitted (MAR-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-11 AND 271-279, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, MASS SPECTROMETRY. Strain: C57BL/6J. Tissue: Skeletal muscle.
[9]	Lubec G., Klug S., Yang J.W., Zigmond M. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 226-317, MASS SPECTROMETRY. Tissue: Brain and Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D29802 mRNA. Translation: BAA06185.1. X75313 mRNA. Translation: CAA53062.1. AK002493 mRNA. Translation: BAB22141.1. AK012242 mRNA. Translation: BAB28114.1. AK017772 mRNA. Translation: BAB30920.1. AK051226 mRNA. Translation: BAC34564.1. AK159797 mRNA. Translation: BAE35378.1. AK160528 mRNA. Translation: BAE35846.1. AK160739 mRNA. Translation: BAE35980.1. AK161234 mRNA. Translation: BAE36257.1. AK166417 mRNA. Translation: BAE38762.1. AK166786 mRNA. Translation: BAE39017.1. AK166800 mRNA. Translation: BAE39028.1. AK166889 mRNA. Translation: BAE39095.1. AK166945 mRNA. Translation: BAE39133.1. AK168086 mRNA. Translation: BAE40059.1. AK168096 mRNA. Translation: BAE40068.1. AK168196 mRNA. Translation: BAE40156.1. AK168349 mRNA. Translation: BAE40286.1. AL645849 Genomic DNA. Translation: CAI35106.1. CH466575 Genomic DNA. Translation: EDL33791.1. BC046760 mRNA. Translation: AAH46760.1. AF295529 Genomic DNA. Translation: AAG29506.1. Frameshift.
IPI	IPI00317740.
PIR	S38398.
RefSeq	NP_032169.1.
UniGene	Mm.5305
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P68040. 8 interactions.
STRING	P68040.
PTM databases
PhosphoSite	P68040.
2-D gel databases
SWISS-2DPAGE	P68040.
REPRODUCTION-2DPAGE	P68040.
Proteomic databases
PRIDE	P68040.
Genome annotation databases
Ensembl	ENSMUST00000020640; ENSMUSP00000020640; ENSMUSG00000020372; Mus musculus. [Genome view]
GeneID	14694.
KEGG	mmu:14694.
UCSC	uc007ipa.1. mouse.
Organism-specific databases
CTD	14694.
MGI	MGI:101849. Gnb2l1.
Phylogenomic databases
HOGENOM	P68040.
HOVERGEN	P68040.
OMA	NHFVSDV
Gene expression databases
ArrayExpress	P68040.
Bgee	P68040.
Genevestigator	P68040.
GermOnline	ENSMUSG00000020372. Mus musculus.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep_reg. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00400. WD40. 7 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. 4 hits. PS50082. WD_REPEATS_2. 6 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	286639.
SOURCE	Search...

Entry information

Entry name

GBLP_MOUSE

Accession

Primary (citable) accession number: P68040
Secondary accession number(s): P25388 , P99049, Q3THP0, Q3THY7, Q3TKQ0, Q3TW88, Q5NCC5, Q5NCC6, Q9CSQ0, Q9ERM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)