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Protein

Receptor of activated protein C kinase 1

Gene

Rack1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (By similarity).By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Apoptosis, Biological rhythms, Cell cycle, Gastrulation, Growth regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-5357905. Regulation of TNFR1 signaling.
R-MMU-5357956. TNFR1-induced NFkappaB signaling pathway.
R-MMU-5626978. TNFR1-mediated ceramide production.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor of activated protein C kinase 1
Alternative name(s):
12-3
Guanine nucleotide-binding protein subunit beta-2-like 1
Receptor for activated C kinase
Receptor of activated protein kinase C 1
p205
Cleaved into the following chain:
Alternative name(s):
Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed
Gene namesi
Name:Rack1Imported
Synonyms:Gnb2-rs1, Gnb2l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:101849. Rack1.

Subcellular locationi

  • Cell membrane By similarity; Peripheral membrane protein By similarity
  • Cytoplasm 1 Publication
  • Cytoplasmperinuclear region By similarity
  • Nucleus 1 Publication
  • Perikaryon 1 Publication
  • Cell projectiondendrite 1 Publication

  • Note: Recruited to the plasma membrane through interaction with KRT1 which binds to membrane-bound ITGB1. PKC activation induces translocation from the perinuclear region to the cell periphery (By similarity). In the brain, detected mainly in cell bodies and dendrites with little expression in axonal fibers or nuclei (PubMed:16414032).By similarity1 Publication

GO - Cellular componenti

  • cell body Source: ParkinsonsUK-UCL
  • cell-cell adherens junction Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • dendrite Source: UniProtKB
  • extracellular exosome Source: MGI
  • IRE1-RACK1-PP2A complex Source: MGI
  • midbody Source: UniProtKB
  • mitochondrion Source: MGI
  • neuronal cell body Source: MGI
  • neuron projection Source: ParkinsonsUK-UCL
  • nucleus Source: UniProtKB
  • perikaryon Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB
  • phagocytic cup Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004244821 – 317Receptor of activated protein C kinase 1Add BLAST317
Initiator methionineiRemoved; alternate1 Publication
ChainiPRO_00001277322 – 317Receptor of activated protein C kinase 1, N-terminally processedAdd BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed1 Publication1
Modified residuei6PhosphothreonineBy similarity1
Modified residuei10PhosphothreonineBy similarity1
Modified residuei52Phosphotyrosine; by ABL1By similarity1
Modified residuei96PhosphothreonineBy similarity1
Modified residuei130N6-acetyllysineCombined sources1
Modified residuei183N6-acetyllysineCombined sources1
Modified residuei228PhosphotyrosineBy similarity1
Modified residuei276PhosphoserineBy similarity1
Modified residuei316PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP68040.
MaxQBiP68040.
PaxDbiP68040.
PeptideAtlasiP68040.
PRIDEiP68040.
TopDownProteomicsiP68040.

2D gel databases

REPRODUCTION-2DPAGEP68040.
SWISS-2DPAGEP68040.

PTM databases

iPTMnetiP68040.
PhosphoSitePlusiP68040.
SwissPalmiP68040.

Expressioni

Tissue specificityi

Strongly and ubiquitously expressed in the embryonic and early postnatal brain. At E11.5, expressed in a high-dorsal to low-ventral gradient throughout the brain. At E13.5, most abundant in the telecephalon. At E18.5, expressed most abundantly in layers 1-4 of the cortex, striatum, hippocampus, dentate gyrus, and specific thalamic nuclei. This expression decreases during postnatal development and is localized in the dentate gyrus, habenula, piriform cortex, paraventricular nucleus of the hypothalamus and supraoptic nucleus of the adult brain.2 Publications

Developmental stagei

Expressed throughout embryonic brain development with high levels detected at E11.5, E13.5 and E18.5. Also detected at high levels in the adult brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000020372.
GenevisibleiP68040. MM.

Interactioni

Subunit structurei

Interacts with CPNE3 (By similarity). May interact with ABCB4 (By similarity). Component of the small (40S) ribosomal subunit. Interacts with LARP4B. Interacts with PKD2L1 (By similarity). Exists as a monomer and also forms oligomers. Binds SLC9A3R1. Forms a ternary complex with TRIM63 and PRKCE. Interacts with HABP4, KRT1 and OTUB1. Interacts with SRC (via SH2 domain); the interaction is enhanced by tyrosine phosphorylation of RACK1. Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and PRKCA. Interacts with AR. Interacts with IGF1R but not with INSR. Interacts with ADAM12. Interacts with CLEC1B (via N-terminal region) and with HIF1A; the interaction promotes their degradation. Interacts with RHOA; this enhances RHOA activation and promotes cell migration. Interacts with CHRM2; the interaction regulates CHRM2 internalization. Interacts with TRPM6 (via kinase domain). Interacts with PTK2/FAK1; required for PTK2/FAK1 phosphorylation and dephosphorylation. Interacts with FLT1. Interacts with HRAS.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IkbkbO883514EBI-296749,EBI-447960

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199978. 8 interactors.
IntActiP68040. 21 interactors.
MINTiMINT-248042.
STRINGi10090.ENSMUSP00000020640.

Structurei

3D structure databases

ProteinModelPortaliP68040.
SMRiP68040.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati13 – 44WD 1Add BLAST32
Repeati61 – 91WD 2Add BLAST31
Repeati103 – 133WD 3Add BLAST31
Repeati146 – 178WD 4Add BLAST33
Repeati190 – 220WD 5Add BLAST31
Repeati231 – 260WD 6Add BLAST30
Repeati281 – 311WD 7Add BLAST31

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0279. Eukaryota.
ENOG410XQGZ. LUCA.
GeneTreeiENSGT00840000129847.
HOVERGENiHBG000277.
InParanoidiP68040.
KOiK14753.
OMAiATSMENP.
OrthoDBiEOG091G0KZQ.
PhylomeDBiP68040.
TreeFamiTF300600.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET
60 70 80 90 100
NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR
110 120 130 140 150
FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW
160 170 180 190 200
VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA NCKLKTNHIG HTGYLNTVTV
210 220 230 240 250
SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA
260 270 280 290 300
ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA
310
GYTDNLVRVW QVTIGTR
Length:317
Mass (Da):35,077
Last modified:January 23, 2007 - v3
Checksum:i257F91E369ED2044
GO

Sequence cautioni

The sequence AAG29506 differs from that shown. Reason: Frameshift at positions 232 and 239.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9G → R in BAE40068 (PubMed:16141072).Curated1
Sequence conflicti9G → R in BAE40156 (PubMed:16141072).Curated1
Sequence conflicti125R → Q in BAE40059 (PubMed:16141072).Curated1
Sequence conflicti125R → Q in BAE40068 (PubMed:16141072).Curated1
Sequence conflicti125R → Q in BAE40156 (PubMed:16141072).Curated1
Sequence conflicti248L → P in BAE35378 (PubMed:16141072).Curated1
Sequence conflicti266I → M in AAG29506 (PubMed:15489334).Curated1
Sequence conflicti273E → A in BAA06185 (PubMed:7968370).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29802 mRNA. Translation: BAA06185.1.
X75313 mRNA. Translation: CAA53062.1.
AK002493 mRNA. Translation: BAB22141.1.
AK012242 mRNA. Translation: BAB28114.1.
AK017772 mRNA. Translation: BAB30920.1.
AK051226 mRNA. Translation: BAC34564.1.
AK159797 mRNA. Translation: BAE35378.1.
AK160528 mRNA. Translation: BAE35846.1.
AK160739 mRNA. Translation: BAE35980.1.
AK161234 mRNA. Translation: BAE36257.1.
AK166417 mRNA. Translation: BAE38762.1.
AK166786 mRNA. Translation: BAE39017.1.
AK166800 mRNA. Translation: BAE39028.1.
AK166889 mRNA. Translation: BAE39095.1.
AK166945 mRNA. Translation: BAE39133.1.
AK168086 mRNA. Translation: BAE40059.1.
AK168096 mRNA. Translation: BAE40068.1.
AK168196 mRNA. Translation: BAE40156.1.
AK168349 mRNA. Translation: BAE40286.1.
AL645849 Genomic DNA. Translation: CAI35106.1.
CH466575 Genomic DNA. Translation: EDL33791.1.
BC046760 mRNA. Translation: AAH46760.1.
AF295529 Genomic DNA. Translation: AAG29506.1. Frameshift.
CCDSiCCDS24585.1.
PIRiS38398.
RefSeqiNP_032169.1. NM_008143.3.
UniGeneiMm.5305.

Genome annotation databases

EnsembliENSMUST00000020640; ENSMUSP00000020640; ENSMUSG00000020372.
GeneIDi14694.
KEGGimmu:14694.
UCSCiuc007ipa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29802 mRNA. Translation: BAA06185.1.
X75313 mRNA. Translation: CAA53062.1.
AK002493 mRNA. Translation: BAB22141.1.
AK012242 mRNA. Translation: BAB28114.1.
AK017772 mRNA. Translation: BAB30920.1.
AK051226 mRNA. Translation: BAC34564.1.
AK159797 mRNA. Translation: BAE35378.1.
AK160528 mRNA. Translation: BAE35846.1.
AK160739 mRNA. Translation: BAE35980.1.
AK161234 mRNA. Translation: BAE36257.1.
AK166417 mRNA. Translation: BAE38762.1.
AK166786 mRNA. Translation: BAE39017.1.
AK166800 mRNA. Translation: BAE39028.1.
AK166889 mRNA. Translation: BAE39095.1.
AK166945 mRNA. Translation: BAE39133.1.
AK168086 mRNA. Translation: BAE40059.1.
AK168096 mRNA. Translation: BAE40068.1.
AK168196 mRNA. Translation: BAE40156.1.
AK168349 mRNA. Translation: BAE40286.1.
AL645849 Genomic DNA. Translation: CAI35106.1.
CH466575 Genomic DNA. Translation: EDL33791.1.
BC046760 mRNA. Translation: AAH46760.1.
AF295529 Genomic DNA. Translation: AAG29506.1. Frameshift.
CCDSiCCDS24585.1.
PIRiS38398.
RefSeqiNP_032169.1. NM_008143.3.
UniGeneiMm.5305.

3D structure databases

ProteinModelPortaliP68040.
SMRiP68040.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199978. 8 interactors.
IntActiP68040. 21 interactors.
MINTiMINT-248042.
STRINGi10090.ENSMUSP00000020640.

PTM databases

iPTMnetiP68040.
PhosphoSitePlusiP68040.
SwissPalmiP68040.

2D gel databases

REPRODUCTION-2DPAGEP68040.
SWISS-2DPAGEP68040.

Proteomic databases

EPDiP68040.
MaxQBiP68040.
PaxDbiP68040.
PeptideAtlasiP68040.
PRIDEiP68040.
TopDownProteomicsiP68040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020640; ENSMUSP00000020640; ENSMUSG00000020372.
GeneIDi14694.
KEGGimmu:14694.
UCSCiuc007ipa.1. mouse.

Organism-specific databases

CTDi10399.
MGIiMGI:101849. Rack1.

Phylogenomic databases

eggNOGiKOG0279. Eukaryota.
ENOG410XQGZ. LUCA.
GeneTreeiENSGT00840000129847.
HOVERGENiHBG000277.
InParanoidiP68040.
KOiK14753.
OMAiATSMENP.
OrthoDBiEOG091G0KZQ.
PhylomeDBiP68040.
TreeFamiTF300600.

Enzyme and pathway databases

ReactomeiR-MMU-5357905. Regulation of TNFR1 signaling.
R-MMU-5357956. TNFR1-induced NFkappaB signaling pathway.
R-MMU-5626978. TNFR1-mediated ceramide production.

Miscellaneous databases

ChiTaRSiGnb2l1. mouse.
PROiP68040.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020372.
GenevisibleiP68040. MM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRACK1_MOUSE
AccessioniPrimary (citable) accession number: P68040
Secondary accession number(s): P25388
, P99049, Q3THP0, Q3THY7, Q3TKQ0, Q3TW88, Q5NCC5, Q5NCC6, Q9CSQ0, Q9ERM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.