P67981 (MT2_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metallothionein-2
Short name=MT-2

Alternative name(s):
Metallothionein-II
Short name=MT-II

Gene names

Name:

MT2

Organism

Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster
PTM	Acetylation
Gene Ontology (GO)
Biological process	negative regulation of growth Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Metallothionein-2		PRO_0000197201
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Divalent metal cation; cluster B
	Metal binding	7	1	Divalent metal cation; cluster B
	Metal binding	13	1	Divalent metal cation; cluster B
	Metal binding	15	1	Divalent metal cation; cluster B
	Metal binding	19	1	Divalent metal cation; cluster B
	Metal binding	21	1	Divalent metal cation; cluster B
	Metal binding	24	1	Divalent metal cation; cluster B
	Metal binding	26	1	Divalent metal cation; cluster B
	Metal binding	29	1	Divalent metal cation; cluster B
	Metal binding	33	1	Divalent metal cation; cluster A
	Metal binding	34	1	Divalent metal cation; cluster A
	Metal binding	36	1	Divalent metal cation; cluster A
	Metal binding	37	1	Divalent metal cation; cluster A
	Metal binding	41	1	Divalent metal cation; cluster A
	Metal binding	44	1	Divalent metal cation; cluster A
	Metal binding	48	1	Divalent metal cation; cluster A
	Metal binding	50	1	Divalent metal cation; cluster A
	Metal binding	57	1	Divalent metal cation; cluster A
	Metal binding	59	1	Divalent metal cation; cluster A
	Metal binding	60	1	Divalent metal cation; cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	51	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67981 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 600D4243AD4E14F4	FASTA	61	6,146
	10 20 30 40 50 60 MDPNCSCATD GSCSCAGSCK CKECKCTTCK KSCCSCCPVG CAKCSQGCVC KEASDKCSCC A

References

[1]	"cDNA cloning and nucleotide sequence comparison of Chinese hamster metallothionein I and II mRNAs." Griffith B.B., Walters R.A., Enger M.D., Hildebrand C.E., Griffith J.K. Nucleic Acids Res. 11:901-910(1983) [PubMed: 6687636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of Chinese hamster metallothionein II gene and its 5' flanking region." Hung S.H., Yu C.W., Chou T.M., Huang P.C., Lin L.Y. Biochim. Biophys. Acta 1090:255-258(1991) [PubMed: 1932120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Sequence homology of Chinese hamster metallothionein genes I and II to those of the mouse and rat, and their amplification in Cd-resistant cells." Yamada K., Kato H., Kanda N., Fujii-Kuriyama Y., Utakoji T., Itoh R. Biochim. Biophys. Acta 1219:581-591(1994) [PubMed: 7948015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lung.
[4]	"Splicing patterns of the Chinese hamster metallothionein II gene transcript." Grady D.L., Hildebrand C.E., Jackson P.J., Walters R.A., Moyzis R.K. Submitted (OCT-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J00062 mRNA. Translation: AAA36997.1. M81319 Genomic DNA. Translation: AAA73166.1. D10552 Genomic DNA. Translation: BAA01409.1. X55065 Genomic DNA. Translation: CAA38898.1.
PIR	SMHY2C. A03276.
RefSeq	NP_001231506.1. NM_001244577.1.
3D structure databases
ProteinModelPortal	P67981.
SMR	P67981. Positions 1-61.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100689479.
Phylogenomic databases
HOVERGEN	HBG096103.
Family and domain databases
InterPro	IPR017854. Metalthion_dom. IPR023587. Metalthion_dom_vert. IPR003019. Metalthion_sfam_euk. IPR000006. Metalthion_vert. IPR018064. Metalthion_vert_metal_BS. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
SUPFAM	SSF57868. Metallothionein_sfam. 1 hit.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MT2_CRIGR

Accession

Primary (citable) accession number: P67981
Secondary accession number(s): P02799

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program