Reviewed,
UniProtKB/Swiss-Prot P67981 (MT2_CRIGR)
Last modified
November 24, 2009.
Version 27.
History...
Clusters with 100%,
90%,
50% identity |
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Metallothionein-2 Short name=MT-2 Alternative name(s): Metallothionein-II Short name=MT-II | ||
| Gene names |
| ||
| Organism | Cricetulus griseus (Chinese hamster) | ||
| Taxonomic identifier | 10029 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus |
Protein attributes
| Sequence length | 61 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. |
| Domain | Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines. |
| Sequence similarities | Belongs to the metallothionein superfamily. Type 1 family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Metal-binding Metal-thiolate cluster |
| PTM | Acetylation |
| Gene Ontology (GO) | |
| Molecular function | metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 61 | 61 | Metallothionein-2 | PRO_0000197201 | |||||
Regions | |||||||||
| Region | 1 – 29 | 29 | Beta | ||||||
| Region | 30 – 61 | 32 | Alpha | ||||||
Sites | |||||||||
| Metal binding | 5 | 1 | Cluster B | ||||||
| Metal binding | 7 | 1 | Cluster B | ||||||
| Metal binding | 13 | 1 | Cluster B | ||||||
| Metal binding | 15 | 1 | Cluster B | ||||||
| Metal binding | 19 | 1 | Cluster B | ||||||
| Metal binding | 21 | 1 | Cluster B | ||||||
| Metal binding | 24 | 1 | Cluster B | ||||||
| Metal binding | 26 | 1 | Cluster B | ||||||
| Metal binding | 29 | 1 | Cluster B | ||||||
| Metal binding | 33 | 1 | Cluster A | ||||||
| Metal binding | 34 | 1 | Cluster A | ||||||
| Metal binding | 36 | 1 | Cluster A | ||||||
| Metal binding | 37 | 1 | Cluster A | ||||||
| Metal binding | 41 | 1 | Cluster A | ||||||
| Metal binding | 44 | 1 | Cluster A | ||||||
| Metal binding | 48 | 1 | Cluster A | ||||||
| Metal binding | 50 | 1 | Cluster A | ||||||
| Metal binding | 57 | 1 | Cluster A | ||||||
| Metal binding | 59 | 1 | Cluster A | ||||||
| Metal binding | 60 | 1 | Cluster A | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine By similarity | ||||||
| Modified residue | 51 | 1 | N6-acetyllysine By similarity | ||||||
Sequences
References
| [1] | "cDNA cloning and nucleotide sequence comparison of Chinese hamster metallothionein I and II mRNAs." Griffith B.B., Walters R.A., Enger M.D., Hildebrand C.E., Griffith J.K. Nucleic Acids Res. 11:901-910(1983) [PubMed: 6687636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Molecular cloning of Chinese hamster metallothionein II gene and its 5' flanking region." Hung S.H., Yu C.W., Chou T.M., Huang P.C., Lin L.Y. Biochim. Biophys. Acta 1090:255-258(1991) [PubMed: 1932120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. |
| [3] | "Sequence homology of Chinese hamster metallothionein genes I and II to those of the mouse and rat, and their amplification in Cd-resistant cells." Yamada K., Kato H., Kanda N., Fujii-Kuriyama Y., Utakoji T., Itoh R. Biochim. Biophys. Acta 1219:581-591(1994) [PubMed: 7948015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lung. |
| [4] | "Splicing patterns of the Chinese hamster metallothionein II gene transcript." Grady D.L., Hildebrand C.E., Jackson P.J., Walters R.A., Moyzis R.K. Submitted (OCT-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. |
Cross-references
Sequence databases | |
|---|---|
| J00062 mRNA. Translation: AAA36997.1. M81319 Genomic DNA. Translation: AAA73166.1. D10552 Genomic DNA. Translation: BAA01409.1. X55065 Genomic DNA. Translation: CAA38898.1. | |
| PIR | SMHY2C. A03276. |
3D structure databases | |
| SMR | P67981. Positions 1-61. |
| ModBase | Search... |
Phylogenomic databases | |
| HOVERGEN | P67981. |
Family and domain databases | |
| InterPro | IPR017854. Metallothionein_sfam. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. IPR018064. Metallothionein_vert_metal_BS. [Graphical view] |
| Gene3D | G3DSA:4.10.10.10. Metallothionein_vert. 1 hit. |
| PANTHER | PTHR23299. Metallothionein_vert. 1 hit. |
| Pfam | PF00131. Metallothio. 1 hit. [Graphical view] |
| PRINTS | PR00860. MTVERTEBRATE. |
| PROSITE | PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MT2_CRIGR | ||||||||
| Accession | Primary (citable) accession number: P67981 Secondary accession number(s): P02799 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Metallothioneins Classification of metallothioneins and list of entries |
| SIMILARITY comments Index of protein domains and families |
