Reviewed, UniProtKB/Swiss-Prot P67936 (TPM4_HUMAN)

Names and origin

Protein names

Recommended name:
    Tropomyosin alpha-4 chain
Alternative name(s):
    Tropomyosin-4
    TM30p1

Gene names

Name:

TPM4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Subcellular location	Cytoplasm › cytoskeleton.
Domain	The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
Sequence similarities	Belongs to the tropomyosin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Ligand	Actin-binding
Molecular function	Muscle protein
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell motion Traceable author statement. Source: UniProtKB
Cellular component	muscle thin filament tropomyosin Traceable author statement. Source: ProtInc
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of muscle Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P67936-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P67936-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: MAGLNSLEAV...LDGERERREK → MEAIKKKMQM...LELTEKKASD

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 248	247	Tropomyosin alpha-4 chain		PRO_0000205635
Regions
	Coiled coil	2 – 248	247	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine
	Modified residue	126	1	Phosphotyrosine
Natural variations
	Alternative sequence	1 – 44	44	MAGLN…ERREK → MEAIKKKMQMLKLDKENAID RAEQAEADKKAAEDKCKQVE EELTHLQKKLKGTEDELDKY SEDLKDAQEKLELTEKKASD in isoform 2.		VSP_006611
	Natural variant	204	1	E → Q in a breast cancer sample; somatic mutation.		VAR_036535
Experimental info
	Sequence conflict	59	1	V → F in AAH02827. Ref.3
	Sequence conflict	152	1	L → S in AAA36774. Ref.7
	Sequence conflict	162 – 163	2	KN → RT in AAA36774. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5D5CDF6BF76552A8	FASTA	248	28,522
	10 20 30 40 50 60 MAGLNSLEAV KRKIQALQQQ ADEAEDRAQG LQRELDGERE RREKAEGDVA ALNRRIQLVE 70 80 90 100 110 120 EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRAMK DEEKMEIQEM QLKEAKHIAE 130 140 150 160 170 180 EADRKYEEVA RKLVILEGEL ERAEERAEVS ELKCGDLEEE LKNVTNNLKS LEAASEKYSE 190 200 210 220 230 240 KEDKYEEEIK LLSDKLKEAE TRAEFAERTV AKLEKTIDDL EEKLAQAKEE NVGLHQTLDQ TLNELNCI
	Isoform 2 [UniParc] [UniParc]. Checksum: 29C0F6ADF4C87F0C		284	32,723
	10 20 30 40 50 60 MEAIKKKMQM LKLDKENAID RAEQAEADKK AAEDKCKQVE EELTHLQKKL KGTEDELDKY 70 80 90 100 110 120 SEDLKDAQEK LELTEKKASD AEGDVAALNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 130 140 150 160 170 180 DESERGMKVI ENRAMKDEEK MEIQEMQLKE AKHIAEEADR KYEEVARKLV ILEGELERAE 190 200 210 220 230 240 ERAEVSELKC GDLEEELKNV TNNLKSLEAA SEKYSEKEDK YEEEIKLLSD KLKEAETRAE 250 260 270 280 FAERTVAKLE KTIDDLEEKL AQAKEENVGL HQTLDQTLNE LNCI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a cDNA defining a gene family encoding TM30p1, a human fibroblast tropomyosin." McLeod A.R., Talbot K., Smillie L.B., Houlker C. J. Mol. Biol. 194:1-10(1987) [PubMed: 3612796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Fibroblast.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Ovarian carcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye, Placenta and Testis.
[4]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-12. Tissue: Platelet.
[5]	Bienvenut W.V., Claeys D. Submitted (NOV-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-11; 45-54; 56-69; 77-89; 133-146 AND 216-223, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Platelet.
[6]	Lubec G., Afjehi-Sadat L. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 56-65 AND 132-142, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[7]	"A muscle-type tropomyosin in human fibroblasts: evidence for expression by an alternative RNA splicing mechanism." MacLeod A.R., Houlker C., Reinach F.C., Smillie L.B., Talbot K., Modi G., Walsh F.S. Proc. Natl. Acad. Sci. U.S.A. 82:7835-7839(1985) [PubMed: 3865200] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 118-248. Tissue: Fibroblast.
[8]	"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes." Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J. Electrophoresis 13:960-969(1992) [PubMed: 1286667] [Abstract] Cited for: PROTEIN SEQUENCE OF 177-189 AND 228-247. Tissue: Keratinocyte.
[9]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-126, MASS SPECTROMETRY.
[10]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-204.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	X05276 mRNA. Translation: CAA28888.1. AK023385 mRNA. Translation: BAB14554.1. BC002827 mRNA. Translation: AAH02827.1. BC037576 mRNA. Translation: AAH37576.1. BC067225 mRNA. Translation: AAH67225.1. M12127 mRNA. Translation: AAA36774.1.
PIR	S07282.
RefSeq	NP_003281.1.
UniGene	Hs.631618
3D structure databases
HSSP	HSSP built from PDB template 1C1G based on UniProtKB P42639.
ModBase	Search...
PTM databases
PhosphoSite	P67936.
2-D gel databases
Aarhus/Ghent-2DPAGE	9122. IEF.
DOSAC-COBS-2DPAGE	P67936.
OGP	P07226.
Proteomic databases
PeptideAtlas	P67936.
Genome annotation databases
Ensembl	ENSG00000167460. Homo sapiens. [Contig view]
GeneID	7171.
KEGG	hsa:7171.
Organism-specific databases
H-InvDB	HIX0014859. HIX0022609. HIX0055475.
HGNC	HGNC:12013. TPM4.
HPA	CAB004685.
MIM	600317. gene.
PharmGKB	PA36693.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	P67936.
Gene expression databases
ArrayExpress	P67936.
CleanEx	HS_TPM4.
GermOnline	ENSG00000167460. Homo sapiens.
Family and domain databases
InterPro	IPR000533. Tropomyosin. [Graphical view]
Pfam	PF00261. Tropomyosin. 1 hit. [Graphical view]
PRINTS	PR00194. TROPOMYOSIN.
PROSITE	PS00326. TROPOMYOSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P67936.
NextBio	28104.
SOURCE	Search...

Entry information

Entry name

TPM4_HUMAN

Accession

Primary (citable) accession number: P67936
Secondary accession number(s): P07226 , Q15659, Q9BU85, Q9H8Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 49 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)