Reviewed, UniProtKB/Swiss-Prot P67918 (BLA1_HAEIN)

Names and origin

Protein names

Recommended name:
    Beta-lactamase ROB-1
    EC=3.5.2.6

Gene names

Name:	rob1
Synonyms:	bla

Encoded on

Plasmid RRob Ref.1

Organism

Haemophilus influenzae

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 305	272	Beta-lactamase ROB-1		PRO_0000017040
Regions
	Region	248 – 250	3	Substrate binding By similarity
Sites
	Active site	86	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67918-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6146E12B76607422	FASTA	305	33,869
	10 20 30 40 50 60 MLNKLKIGTL LLLTLTACSP NSVHSVTSNP QPASAPVQQS ATQATFQQTL ANLEQQYQAR 70 80 90 100 110 120 IGVYVWDTET GHSLSYRADE RFAYASTFKA LLAGAVLQSL PEKDLNRTIS YSQKDLVSYS 130 140 150 160 170 180 PETQKYVGKG MTIAQLCEAA VRFSDNSATN LLLKELGGVE QYQRILRQLG DNVTHTNRLE 190 200 210 220 230 240 PDLNQAKPND IRDTSTPKQM AMNLNAYLLG NTLTESQKTI LWNWLDNNAT GNPLIRAATP 250 260 270 280 290 300 TSWKVYDKSG AGKYGVRNDI AVVRIPNRKP IVMAIMSTQF TEEAKFNNKL VEDAAKQVFH TLQLN

References

[1]	"Sequence analysis and evolutionary perspectives of ROB-1 beta-lactamase." Juteau J.-M., Levesque R.C. Antimicrob. Agents Chemother. 34:1354-1359(1990) [PubMed: 2201253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: F990.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF022114 Genomic DNA. Translation: AAB72007.1.
PIR	A60680.
3D structure databases
HSSP	HSSP built from PDB template 1I2W based on UniProtKB P00808.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 109.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA1_HAEIN

Accession

Primary (citable) accession number: P67918
Secondary accession number(s): P33949

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)