Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonuclease mitogillin

Gene

ret

Organism
Aspergillus restrictus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This purine-specific ribonuclease cleaves 28S RNA in eukaryotic ribosomes, inhibits protein synthesis, and shows antitumor activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei761
Active sitei122Proton acceptor1
Active sitei163Proton donor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease, Protein synthesis inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease mitogillin (EC:3.1.27.-)
Alternative name(s):
Restrictocin
Gene namesi
Name:ret
OrganismiAspergillus restrictus
Taxonomic identifieri5064 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei3050. Asp r 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000003083828 – 176Ribonuclease mitogillinAdd BLAST149

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 174
Disulfide bondi102 ↔ 158

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 35Combined sources7
Turni39 – 42Combined sources4
Beta strandi46 – 52Combined sources7
Helixi53 – 62Combined sources10
Beta strandi65 – 68Combined sources4
Beta strandi70 – 72Combined sources3
Beta strandi75 – 78Combined sources4
Beta strandi84 – 86Combined sources3
Helixi100 – 103Combined sources4
Beta strandi118 – 124Combined sources7
Turni137 – 140Combined sources4
Beta strandi145 – 152Combined sources8
Beta strandi157 – 165Combined sources9
Beta strandi171 – 173Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AQZX-ray1.70A/B28-176[»]
1JBRX-ray2.15A/B28-176[»]
1JBSX-ray1.97A/B28-176[»]
1JBTX-ray2.70A/B28-176[»]
ProteinModelPortaliP67876.
SMRiP67876.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP67876.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease U2 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PIRSFiPIRSF037430. RNase_U2. 1 hit.
PRINTSiPR01704. FUNRIBOTOXIN.
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P67876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAIKNLFLL AATAVSVLAA PSPLDARATW TCINQQLNPK TNKWEDKRLL
60 70 80 90 100
YSQAKAESNS HHAPLSDGKT GSSYPHWFTN GYDGNGKLIK GRTPIKFGKA
110 120 130 140 150
DCDRPPKHSQ NGMGKDDHYL LEFPTFPDGH DYKFDSKKPK EDPGPARVIY
160 170
TYPNKVFCGI VAHQRGNQGD LRLCSH
Length:176
Mass (Da):19,595
Last modified:October 11, 2004 - v1
Checksum:iAAD431C57939E42C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52S → N in AAA32707 (Ref. 3) Curated1
Sequence conflicti142D → N AA sequence (PubMed:3994994).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56176 Genomic DNA. Translation: CAA39637.1.
M55508 mRNA. Translation: AAA32706.1.
M65257 Genomic DNA. Translation: AAA32707.1.
PIRiS22294. NRASMR.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56176 Genomic DNA. Translation: CAA39637.1.
M55508 mRNA. Translation: AAA32706.1.
M65257 Genomic DNA. Translation: AAA32707.1.
PIRiS22294. NRASMR.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AQZX-ray1.70A/B28-176[»]
1JBRX-ray2.15A/B28-176[»]
1JBSX-ray1.97A/B28-176[»]
1JBTX-ray2.70A/B28-176[»]
ProteinModelPortaliP67876.
SMRiP67876.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3050. Asp r 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP67876.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PIRSFiPIRSF037430. RNase_U2. 1 hit.
PRINTSiPR01704. FUNRIBOTOXIN.
SUPFAMiSSF53933. SSF53933. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNMG_ASPRE
AccessioniPrimary (citable) accession number: P67876
Secondary accession number(s): P04389, P19792
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.