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P67876

- RNMG_ASPRE

UniProt

P67876 - RNMG_ASPRE

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Protein

Ribonuclease mitogillin

Gene
ret
Organism
Aspergillus restrictus
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This purine-specific ribonuclease cleaves 28S RNA in eukaryotic ribosomes, inhibits protein synthesis, and shows antitumor activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei76 – 761
Active sitei122 – 1221Proton acceptor
Active sitei163 – 1631Proton donor

GO - Molecular functioni

  1. endoribonuclease activity Source: InterPro
  2. RNA binding Source: InterPro

GO - Biological processi

  1. negative regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease, Protein synthesis inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease mitogillin (EC:3.1.27.-)
Alternative name(s):
Restrictocin
Gene namesi
Name:ret
OrganismiAspergillus restrictus
Taxonomic identifieri5064 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei3050. Asp r 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 176149Ribonuclease mitogillinPRO_0000030838Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 174
Disulfide bondi102 ↔ 158

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
176
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 357
Turni39 – 424
Beta strandi46 – 527
Helixi53 – 6210
Beta strandi65 – 684
Beta strandi70 – 723
Beta strandi75 – 784
Beta strandi84 – 863
Helixi100 – 1034
Beta strandi118 – 1247
Turni137 – 1404
Beta strandi145 – 1528
Beta strandi157 – 1659
Beta strandi171 – 1733

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AQZX-ray1.70A/B28-176[»]
1JBRX-ray2.15A/B28-176[»]
1JBSX-ray1.97A/B28-176[»]
1JBTX-ray2.70A/B28-176[»]
ProteinModelPortaliP67876.
SMRiP67876. Positions 28-176.

Miscellaneous databases

EvolutionaryTraceiP67876.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease U2 family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
PIRSFiPIRSF037430. RNase_U2. 1 hit.
PRINTSiPR01704. FUNRIBOTOXIN.
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P67876-1 [UniParc]FASTAAdd to Basket

« Hide

MVAIKNLFLL AATAVSVLAA PSPLDARATW TCINQQLNPK TNKWEDKRLL    50
YSQAKAESNS HHAPLSDGKT GSSYPHWFTN GYDGNGKLIK GRTPIKFGKA 100
DCDRPPKHSQ NGMGKDDHYL LEFPTFPDGH DYKFDSKKPK EDPGPARVIY 150
TYPNKVFCGI VAHQRGNQGD LRLCSH 176
Length:176
Mass (Da):19,595
Last modified:October 11, 2004 - v1
Checksum:iAAD431C57939E42C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521S → N in AAA32707. 1 Publication
Sequence conflicti142 – 1421D → N AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56176 Genomic DNA. Translation: CAA39637.1.
M55508 mRNA. Translation: AAA32706.1.
M65257 Genomic DNA. Translation: AAA32707.1.
PIRiS22294. NRASMR.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56176 Genomic DNA. Translation: CAA39637.1 .
M55508 mRNA. Translation: AAA32706.1 .
M65257 Genomic DNA. Translation: AAA32707.1 .
PIRi S22294. NRASMR.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AQZ X-ray 1.70 A/B 28-176 [» ]
1JBR X-ray 2.15 A/B 28-176 [» ]
1JBS X-ray 1.97 A/B 28-176 [» ]
1JBT X-ray 2.70 A/B 28-176 [» ]
ProteinModelPortali P67876.
SMRi P67876. Positions 28-176.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3050. Asp r 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P67876.

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
PIRSFi PIRSF037430. RNase_U2. 1 hit.
PRINTSi PR01704. FUNRIBOTOXIN.
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation and nucleotide sequence of the Aspergillus restrictus gene coding for the ribonucleolytic toxin restrictocin and its expression in Aspergillus nidulans: the leader sequence protects producing strains from suicide."
    Lamy B., Davies J.
    Nucleic Acids Res. 19:1001-1006(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 34475 / NRRL 2869.
  2. "Secretion of a potential virulence factor, a fungal ribonucleotoxin, during human aspergillosis infections."
    Lamy B., Moutaouakil M., Latge J.P., Davies J.
    Mol. Microbiol. 5:1811-1815(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 34475 / NRRL 2869.
  3. "Regulation and sequence of the restrictocin gene of Aspergillus restrictus."
    Yang R., Kenealy W.R.
    Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: ATCC 34475 / NRRL 2869.
  4. "Complete amino acid sequence of the Aspergillus cytotoxin mitogillin."
    Fernandez-Luna J.L., Lopez-Otin C., Soriano F., Mendez E.
    Biochemistry 24:861-867(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-176.
  5. "Insights into specificity of cleavage and mechanism of cell entry from the crystal structure of the highly specific Aspergillus ribotoxin, restrictocin."
    Yang X., Moffat K.
    Structure 4:837-852(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 28-176.
  6. "Crystal structures of restrictocin-inhibitor complexes with implications for RNA recognition and base flipping."
    Yang X., Gerczei T., Glover L.T., Correll C.C.
    Nat. Struct. Biol. 8:968-973(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 28-176.

Entry informationi

Entry nameiRNMG_ASPRE
AccessioniPrimary (citable) accession number: P67876
Secondary accession number(s): P04389, P19792
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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