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P67875

- RNMG_ASPFU

UniProt

P67875 - RNMG_ASPFU

Protein

Ribonuclease mitogillin

Gene

mitF

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    This purine-specific ribonuclease cleaves 28S RNA in eukaryotic ribosomes, inhibits protein synthesis, and shows antitumor activity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei76 – 761By similarity
    Active sitei122 – 1221Proton acceptorBy similarity
    Active sitei163 – 1631Proton donorBy similarity

    GO - Molecular functioni

    1. endoribonuclease activity Source: InterPro
    2. IgE binding Source: ASPGD
    3. ribonuclease activity Source: ASPGD
    4. RNA binding Source: InterPro

    GO - Biological processi

    1. negative regulation of translation Source: UniProtKB-KW
    2. regulation of cytokine activity Source: ASPGD
    3. RNA phosphodiester bond hydrolysis Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Nuclease, Protein synthesis inhibitor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease mitogillin (EC:3.1.27.-)
    Alternative name(s):
    Allergen Asp f I
    Allergen I/a
    IgE-binding ribotoxin
    Major allergen Asp f 1
    Allergen: Asp f 1
    Gene namesi
    Name:mitF
    Synonyms:aspF1
    ORF Names:AFUA_5G02330
    OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
    Taxonomic identifieri330879 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000002530: Chromosome 5

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. Binds to IgE. May promote colonization through cytotoxic activity and by causing inflammatory reactions involving IgE antibodies. Binds also to IgG.

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei3107. Asp f 1.0101.
    62. Asp f 1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 27271 PublicationAdd
    BLAST
    Chaini28 – 176149Ribonuclease mitogillinPRO_0000030837Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi32 ↔ 174
    Disulfide bondi102 ↔ 158

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP67875.
    SMRiP67875. Positions 28-176.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribonuclease U2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG250988.
    HOGENOMiHOG000216956.
    KOiK16639.
    OMAiSSYPHWF.
    OrthoDBiEOG7HHX4D.

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR004025. Fun_ribotoxin.
    IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037430. RNase_U2. 1 hit.
    PRINTSiPR01704. FUNRIBOTOXIN.
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P67875-1 [UniParc]FASTAAdd to Basket

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    MVAIKNLFLL AATAVSVLAA PSPLDARATW TCINQQLNPK TNKWEDKRLL    50
    YSQAKAESNS HHAPLSDGKT GSSYPHWFTN GYDGNGKLIK GRTPIKFGKA 100
    DCDRPPKHSQ NGMGKDDHYL LEFPTFPDGH DYKFDSKKPK EDPGPARVIY 150
    TYPNKVFCGI VAHQRGNQGD LRLCSH 176
    Length:176
    Mass (Da):19,595
    Last modified:October 11, 2004 - v1
    Checksum:iAAD431C57939E42C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 521S → N in CAA41217. (PubMed:1431110)Curated
    Sequence conflicti52 – 521S → N in AAB07779. (PubMed:1431110)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58278 Genomic DNA. Translation: CAA41217.1.
    M83781 Genomic DNA. Translation: AAB07779.1.
    AAHF01000011 Genomic DNA. Translation: EAL86071.1.
    S39330 mRNA. Translation: AAB22442.1.
    PIRiA46497.
    RefSeqiXP_748109.1. XM_743016.1.

    Genome annotation databases

    EnsemblFungiiCADAFUAT00002637; CADAFUAP00002637; CADAFUAG00002637.
    GeneIDi3505541.
    KEGGiafm:AFUA_5G02330.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58278 Genomic DNA. Translation: CAA41217.1 .
    M83781 Genomic DNA. Translation: AAB07779.1 .
    AAHF01000011 Genomic DNA. Translation: EAL86071.1 .
    S39330 mRNA. Translation: AAB22442.1 .
    PIRi A46497.
    RefSeqi XP_748109.1. XM_743016.1.

    3D structure databases

    ProteinModelPortali P67875.
    SMRi P67875. Positions 28-176.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 3107. Asp f 1.0101.
    62. Asp f 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAFUAT00002637 ; CADAFUAP00002637 ; CADAFUAG00002637 .
    GeneIDi 3505541.
    KEGGi afm:AFUA_5G02330.

    Phylogenomic databases

    eggNOGi NOG250988.
    HOGENOMi HOG000216956.
    KOi K16639.
    OMAi SSYPHWF.
    OrthoDBi EOG7HHX4D.

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR004025. Fun_ribotoxin.
    IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037430. RNase_U2. 1 hit.
    PRINTSi PR01704. FUNRIBOTOXIN.
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Secretion of a potential virulence factor, a fungal ribonucleotoxin, during human aspergillosis infections."
      Lamy B., Moutaouakil M., Latge J.P., Davies J.
      Mol. Microbiol. 5:1811-1815(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CBS 143.89.
    2. "Selective expression of a major allergen and cytotoxin, Asp f I, in Aspergillus fumigatus. Implications for the immunopathogenesis of Aspergillus-related diseases."
      Arruda L.K., Mann B.J., Chapman M.D.
      J. Immunol. 149:3354-3359(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
      Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
      , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
      Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
    4. "Cloning and expression of recombinant Aspergillus fumigatus allergen I/a (rAsp f I/a) with IgE binding and type I skin test activity."
      Moser M., Crameri R., Menz G., Schneider T., Dudler T., Virchow C., Gmachl M., Blaser K., Suter M.
      J. Immunol. 149:454-460(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-176.
    5. "Aspergillus fumigatus allergen I, a major IgE-binding protein, is a member of the mitogillin family of cytotoxins."
      Arruda L.K., Platts-Mills T.A.E., Fox J.W., Chapman M.D.
      J. Exp. Med. 172:1529-1532(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PROTEIN SEQUENCE OF 28-176.
      Strain: 5167.
    6. Sarma P.U., Bir N., Fairwell T.
      Submitted (AUG-1999) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 28-48.
      Strain: AF-285 / Indian isolate.

    Entry informationi

    Entry nameiRNMG_ASPFU
    AccessioniPrimary (citable) accession number: P67875
    Secondary accession number(s): P04389, P19792, Q4WEA6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3