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P67875 (RNMG_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease mitogillin

EC=3.1.27.-
Alternative name(s):
Allergen Asp f I
Allergen I/a
IgE-binding ribotoxin
Major allergen Asp f 1
Allergen=Asp f 1
Gene names
Name:mitF
Synonyms:aspF1
ORF Names:AFUA_5G02330
OrganismNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]
Taxonomic identifier330879 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length176 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This purine-specific ribonuclease cleaves 28S RNA in eukaryotic ribosomes, inhibits protein synthesis, and shows antitumor activity.

Subcellular location

Secreted.

Allergenic properties

Causes an allergic reaction in human. Binds to IgE. May promote colonization through cytotoxic activity and by causing inflammatory reactions involving IgE antibodies. Binds also to IgG.

Sequence similarities

Belongs to the ribonuclease U2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.5 Ref.6
Chain28 – 176149Ribonuclease mitogillin
PRO_0000030837

Sites

Active site761 By similarity
Active site1221Proton acceptor By similarity
Active site1631Proton donor By similarity

Amino acid modifications

Disulfide bond32 ↔ 174
Disulfide bond102 ↔ 158

Experimental info

Sequence conflict521S → N in CAA41217. Ref.2
Sequence conflict521S → N in AAB07779. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P67875 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: AAD431C57939E42C

FASTA17619,595
        10         20         30         40         50         60 
MVAIKNLFLL AATAVSVLAA PSPLDARATW TCINQQLNPK TNKWEDKRLL YSQAKAESNS 

        70         80         90        100        110        120 
HHAPLSDGKT GSSYPHWFTN GYDGNGKLIK GRTPIKFGKA DCDRPPKHSQ NGMGKDDHYL 

       130        140        150        160        170 
LEFPTFPDGH DYKFDSKKPK EDPGPARVIY TYPNKVFCGI VAHQRGNQGD LRLCSH 

« Hide

References

« Hide 'large scale' references
[1]"Secretion of a potential virulence factor, a fungal ribonucleotoxin, during human aspergillosis infections."
Lamy B., Moutaouakil M., Latge J.P., Davies J.
Mol. Microbiol. 5:1811-1815(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CBS 143.89.
[2]"Selective expression of a major allergen and cytotoxin, Asp f I, in Aspergillus fumigatus. Implications for the immunopathogenesis of Aspergillus-related diseases."
Arruda L.K., Mann B.J., Chapman M.D.
J. Immunol. 149:3354-3359(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. expand/collapse author list , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
[4]"Cloning and expression of recombinant Aspergillus fumigatus allergen I/a (rAsp f I/a) with IgE binding and type I skin test activity."
Moser M., Crameri R., Menz G., Schneider T., Dudler T., Virchow C., Gmachl M., Blaser K., Suter M.
J. Immunol. 149:454-460(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-176.
[5]"Aspergillus fumigatus allergen I, a major IgE-binding protein, is a member of the mitogillin family of cytotoxins."
Arruda L.K., Platts-Mills T.A.E., Fox J.W., Chapman M.D.
J. Exp. Med. 172:1529-1532(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY PROTEIN SEQUENCE OF 28-176.
Strain: 5167.
[6]Sarma P.U., Bir N., Fairwell T.
Submitted (AUG-1999) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 28-48.
Strain: AF-285 / Indian isolate.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X58278 Genomic DNA. Translation: CAA41217.1.
M83781 Genomic DNA. Translation: AAB07779.1.
AAHF01000011 Genomic DNA. Translation: EAL86071.1.
S39330 mRNA. Translation: AAB22442.1.
PIRA46497.
RefSeqXP_748109.1. XM_743016.1.

3D structure databases

ProteinModelPortalP67875.
SMRP67875. Positions 28-176.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome3107. Asp f 1.0101.
62. Asp f 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADAFUAT00002637; CADAFUAP00002637; CADAFUAG00002637.
GeneID3505541.
KEGGafm:AFUA_5G02330.

Phylogenomic databases

eggNOGNOG250988.
HOGENOMHOG000216956.
KOK16639.
OMASSYPHWF.
OrthoDBEOG7HHX4D.

Family and domain databases

Gene3D3.10.450.30. 1 hit.
InterProIPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamPF00545. Ribonuclease. 1 hit.
[Graphical view]
PIRSFPIRSF037430. RNase_U2. 1 hit.
PRINTSPR01704. FUNRIBOTOXIN.
SUPFAMSSF53933. SSF53933. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRNMG_ASPFU
AccessionPrimary (citable) accession number: P67875
Secondary accession number(s): P04389, P19792, Q4WEA6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Allergens

Nomenclature of allergens and list of entries