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P67875

- RNMG_ASPFU

UniProt

P67875 - RNMG_ASPFU

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Protein

Ribonuclease mitogillin

Gene

mitF

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This purine-specific ribonuclease cleaves 28S RNA in eukaryotic ribosomes, inhibits protein synthesis, and shows antitumor activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei76 – 761By similarity
Active sitei122 – 1221Proton acceptorBy similarity
Active sitei163 – 1631Proton donorBy similarity

GO - Molecular functioni

  1. endoribonuclease activity Source: InterPro
  2. IgE binding Source: ASPGD
  3. ribonuclease activity Source: ASPGD
  4. RNA binding Source: InterPro

GO - Biological processi

  1. negative regulation of translation Source: UniProtKB-KW
  2. regulation of cytokine activity Source: ASPGD
  3. RNA phosphodiester bond hydrolysis Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease, Protein synthesis inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease mitogillin (EC:3.1.27.-)
Alternative name(s):
Allergen Asp f I
Allergen I/a
IgE-binding ribotoxin
Major allergen Asp f 1
Allergen: Asp f 1
Gene namesi
Name:mitF
Synonyms:aspF1
ORF Names:AFUA_5G02330
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000002530: Chromosome 5

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE. May promote colonization through cytotoxic activity and by causing inflammatory reactions involving IgE antibodies. Binds also to IgG.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3107. Asp f 1.0101.
62. Asp f 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 176149Ribonuclease mitogillinPRO_0000030837Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 174
Disulfide bondi102 ↔ 158

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP67875.
SMRiP67875. Positions 28-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease U2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG250988.
HOGENOMiHOG000216956.
InParanoidiP67875.
KOiK16639.
OMAiSSYPHWF.
OrthoDBiEOG7HHX4D.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
PIRSFiPIRSF037430. RNase_U2. 1 hit.
PRINTSiPR01704. FUNRIBOTOXIN.
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P67875-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVAIKNLFLL AATAVSVLAA PSPLDARATW TCINQQLNPK TNKWEDKRLL
60 70 80 90 100
YSQAKAESNS HHAPLSDGKT GSSYPHWFTN GYDGNGKLIK GRTPIKFGKA
110 120 130 140 150
DCDRPPKHSQ NGMGKDDHYL LEFPTFPDGH DYKFDSKKPK EDPGPARVIY
160 170
TYPNKVFCGI VAHQRGNQGD LRLCSH
Length:176
Mass (Da):19,595
Last modified:October 11, 2004 - v1
Checksum:iAAD431C57939E42C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521S → N in CAA41217. (PubMed:1431110)Curated
Sequence conflicti52 – 521S → N in AAB07779. (PubMed:1431110)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58278 Genomic DNA. Translation: CAA41217.1.
M83781 Genomic DNA. Translation: AAB07779.1.
AAHF01000011 Genomic DNA. Translation: EAL86071.1.
S39330 mRNA. Translation: AAB22442.1.
PIRiA46497.
RefSeqiXP_748109.1. XM_743016.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00002637; CADAFUAP00002637; CADAFUAG00002637.
GeneIDi3505541.
KEGGiafm:AFUA_5G02330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58278 Genomic DNA. Translation: CAA41217.1 .
M83781 Genomic DNA. Translation: AAB07779.1 .
AAHF01000011 Genomic DNA. Translation: EAL86071.1 .
S39330 mRNA. Translation: AAB22442.1 .
PIRi A46497.
RefSeqi XP_748109.1. XM_743016.1.

3D structure databases

ProteinModelPortali P67875.
SMRi P67875. Positions 28-176.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3107. Asp f 1.0101.
62. Asp f 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADAFUAT00002637 ; CADAFUAP00002637 ; CADAFUAG00002637 .
GeneIDi 3505541.
KEGGi afm:AFUA_5G02330.

Phylogenomic databases

eggNOGi NOG250988.
HOGENOMi HOG000216956.
InParanoidi P67875.
KOi K16639.
OMAi SSYPHWF.
OrthoDBi EOG7HHX4D.

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR004025. Fun_ribotoxin.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
PIRSFi PIRSF037430. RNase_U2. 1 hit.
PRINTSi PR01704. FUNRIBOTOXIN.
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Secretion of a potential virulence factor, a fungal ribonucleotoxin, during human aspergillosis infections."
    Lamy B., Moutaouakil M., Latge J.P., Davies J.
    Mol. Microbiol. 5:1811-1815(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CBS 143.89.
  2. "Selective expression of a major allergen and cytotoxin, Asp f I, in Aspergillus fumigatus. Implications for the immunopathogenesis of Aspergillus-related diseases."
    Arruda L.K., Mann B.J., Chapman M.D.
    J. Immunol. 149:3354-3359(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
  4. "Cloning and expression of recombinant Aspergillus fumigatus allergen I/a (rAsp f I/a) with IgE binding and type I skin test activity."
    Moser M., Crameri R., Menz G., Schneider T., Dudler T., Virchow C., Gmachl M., Blaser K., Suter M.
    J. Immunol. 149:454-460(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-176.
  5. "Aspergillus fumigatus allergen I, a major IgE-binding protein, is a member of the mitogillin family of cytotoxins."
    Arruda L.K., Platts-Mills T.A.E., Fox J.W., Chapman M.D.
    J. Exp. Med. 172:1529-1532(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 28-176.
    Strain: 5167.
  6. Sarma P.U., Bir N., Fairwell T.
    Submitted (AUG-1999) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 28-48.
    Strain: AF-285 / Indian isolate.

Entry informationi

Entry nameiRNMG_ASPFU
AccessioniPrimary (citable) accession number: P67875
Secondary accession number(s): P04389, P19792, Q4WEA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: October 11, 2004
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3