P67875 (RNMG_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease mitogillin
EC=3.1.27.-

Alternative name(s):
Allergen Asp f I
Allergen I/a
IgE-binding ribotoxin
Major allergen Asp f 1
Allergen=Asp f 1

Gene names

Name:	mitF
Synonyms:	aspF1
ORF Names:	AFUA_5G02330

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This purine-specific ribonuclease cleaves 28S RNA in eukaryotic ribosomes, inhibits protein synthesis, and shows antitumor activity.
Subcellular location	Secreted.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. May promote colonization through cytotoxic activity and by causing inflammatory reactions involving IgE antibodies. Binds also to IgG.
Sequence similarities	Belongs to the ribonuclease U2 family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Hydrolase Nuclease Protein synthesis inhibitor
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro endoribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.5 Ref.6
	Chain	28 – 176	149	Ribonuclease mitogillin		PRO_0000030837
Sites
	Active site	76	1	By similarity
	Active site	122	1	Proton acceptor By similarity
	Active site	163	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	32 ↔ 174
	Disulfide bond	102 ↔ 158
Experimental info
	Sequence conflict	52	1	S → N in CAA41217. Ref.2
	Sequence conflict	52	1	S → N in AAB07779. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P67875 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: AAD431C57939E42C	FASTA	176	19,595
	10 20 30 40 50 60 MVAIKNLFLL AATAVSVLAA PSPLDARATW TCINQQLNPK TNKWEDKRLL YSQAKAESNS 70 80 90 100 110 120 HHAPLSDGKT GSSYPHWFTN GYDGNGKLIK GRTPIKFGKA DCDRPPKHSQ NGMGKDDHYL 130 140 150 160 170 LEFPTFPDGH DYKFDSKKPK EDPGPARVIY TYPNKVFCGI VAHQRGNQGD LRLCSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Secretion of a potential virulence factor, a fungal ribonucleotoxin, during human aspergillosis infections." Lamy B., Moutaouakil M., Latge J.P., Davies J. Mol. Microbiol. 5:1811-1815(1991) [PubMed: 1943712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CBS 143.89.
[2]	"Selective expression of a major allergen and cytotoxin, Asp f I, in Aspergillus fumigatus. Implications for the immunopathogenesis of Aspergillus-related diseases." Arruda L.K., Mann B.J., Chapman M.D. J. Immunol. 149:3354-3359(1992) [PubMed: 1431110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
[4]	"Cloning and expression of recombinant Aspergillus fumigatus allergen I/a (rAsp f I/a) with IgE binding and type I skin test activity." Moser M., Crameri R., Menz G., Schneider T., Dudler T., Virchow C., Gmachl M., Blaser K., Suter M. J. Immunol. 149:454-460(1992) [PubMed: 1624793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-176.
[5]	"Aspergillus fumigatus allergen I, a major IgE-binding protein, is a member of the mitogillin family of cytotoxins." Arruda L.K., Platts-Mills T.A.E., Fox J.W., Chapman M.D. J. Exp. Med. 172:1529-1532(1990) [PubMed: 2230656] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 28-176. Strain: 5167.
[6]	Sarma P.U., Bir N., Fairwell T. Submitted (AUG-1999) to UniProtKB Cited for: PROTEIN SEQUENCE OF 28-48. Strain: AF-285 / Indian isolate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58278 Genomic DNA. Translation: CAA41217.1. M83781 Genomic DNA. Translation: AAB07779.1. AAHF01000011 Genomic DNA. Translation: EAL86071.1. S39330 mRNA. Translation: AAB22442.1.
PIR	A46497.
RefSeq	XP_748109.1. XM_743016.1.
3D structure databases
ProteinModelPortal	P67875.
SMR	P67875. Positions 28-176.
ModBase	Search...
Protein family/group databases
Allergome	3107. Asp f 1.0101. 62. Asp f 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUAT00002637; CADAFUAP00002637; CADAFUAG00002637.
GeneID	3505541.
GenomeReviews	Gene locus mitF in contig CM000173_GR.
KEGG	afm:AFUA_5G02330.
Phylogenomic databases
OrthoDB	EOG4C5GTQ.
Family and domain databases
InterPro	IPR004025. Fun_ribotoxin. IPR000026. Gua-sp_ribonuclease_N1/T1. IPR016191. Ribonuclease/ribotoxin. [Graphical view]
Gene3D	G3DSA:3.10.450.30. Ribonuclease/ribotoxin. 1 hit.
KO	K01172.
Pfam	PF00545. Ribonuclease. 1 hit. [Graphical view]
PIRSF	PIRSF037430. RNase_U2. 1 hit.
PRINTS	PR01704. FUNRIBOTOXIN.
SUPFAM	SSF53933. Ribonuclease/ribotoxin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNMG_ASPFU

Accession

Primary (citable) accession number: P67875
Secondary accession number(s): P04389, P19792, Q4WEA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program