P67871 (CSK2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Casein kinase II subunit beta
Short name=CK II beta

Alternative name(s):
Phosvitin

Gene names

Name:	Csnk2b
Synonyms:	Ck2n

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a complex role in regulating the basal catalytic activity of the alpha subunit By similarity. Participates in Wnt signaling. Ref.7 Ref.9
Subunit structure	Tetramer composed of an alpha subunit, an alpha' subunit and two beta subunits. The beta subunit dimerization is mediated by zinc ions. Interacts with CD163. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation By similarity. Interacts with TCTEX1D3. Interacts with MUSK; mediates phosphorylation of MUSK by CK2. Interacts with FGF1; this interaction is increased in the presence of FIBP, suggesting a possible cooperative interaction between CSNKB and FIBP in binding to FGF1 By similarity. Ref.8 Ref.9
Post-translational modification	Phosphorylated by alpha subunit By similarity.
Sequence similarities	Belongs to the casein kinase 2 subunit beta family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW regulation of protein kinase activity Inferred from electronic annotation. Source: GOC
Cellular_component	protein kinase CK2 complex Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Tcte3	P11985-2	2	EBI-348179,EBI-1781298

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 215	214	Casein kinase II subunit beta		PRO_0000068237
Regions
	Region	188 – 193	6	Interaction with alpha subunit By similarity
	Compositional bias	55 – 64	10	Asp/Glu-rich (acidic)
Sites
	Metal binding	109	1	Zinc By similarity
	Metal binding	114	1	Zinc By similarity
	Metal binding	137	1	Zinc By similarity
	Metal binding	140	1	Zinc By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	2	1	Phosphoserine; by autocatalysis Probable
	Modified residue	3	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	8	1	Phosphoserine By similarity
	Modified residue	69	1	Phosphoserine By similarity
	Modified residue	209	1	Phosphoserine By similarity
	Modified residue	212	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67871 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: E465B1E699B0E0EC	FASTA	215	24,942
	10 20 30 40 50 60 MSSSEEVSWI SWFCGLRGNE FFCEVDEDYI QDKFNLTGLN EQVPHYRQAL DMILDLEPDE 70 80 90 100 110 120 ELEDNPNQSD LIEQAAEMLY GLIHARYILT NRGIAQMLEK YQQGDFGYCP RVYCENQPML 130 140 150 160 170 180 PIGLSDIPGE AMVKLYCPKC MDVYTPKSSR HHHTDGAYFG TGFPHMLFMV HPEYRPKRPA 190 200 210 NQFVPRLYGF KIHPMAYQLQ LQAASNFKSP VKTIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of the mouse cDNA encoding the beta subunit of casein kinase II." Kopatz I., Naiman T., Eli D., Canaani D. Nucleic Acids Res. 18:3639-3639(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"The beta subunit of casein kinase II: cloning of cDNAs from murine and porcine origin and expression of the porcine sequence as a fusion protein." Boldyreff B., Piontek K., Schmidt-Spaniol I., Issinger O.-J. Biochim. Biophys. Acta 1088:439-441(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure of the gene encoding the murine protein kinase CK2 beta subunit." Boldyreff B., Issinger O.G. Genomics 29:253-256(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6 X CBA. Tissue: Spleen.
[4]	"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse." Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L. Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and DBA/2. Tissue: Kidney and Testis.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary tumor.
[7]	"Endogenous protein kinase CK2 participates in Wnt signaling in mammary epithelial cells." Song D.H., Sussman D.J., Seldin D.C. J. Biol. Chem. 275:23790-23797(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN WNT SIGNALING.
[8]	"The protein of a new gene, Tctex4, interacts with protein kinase CK2beta subunit and is highly expressed in mouse testis." Bai X., Xu X., Zhang F., Shu H.-B., Chan E.D. Biochem. Biophys. Res. Commun. 307:86-91(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TCTEX1D3.
[9]	"Casein kinase 2-dependent serine phosphorylation of MuSK regulates acetylcholine receptor aggregation at the neuromuscular junction." Cheusova T., Khan M.A., Schubert S.W., Gavin A.C., Buchou T., Jacob G., Sticht H., Allende J., Boldyreff B., Brenner H.R., Hashemolhosseini S. Genes Dev. 20:1800-1816(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN PHOSPHORYLATION OF MUSK, INTERACTION WITH MUSK.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52959 mRNA. Translation: CAA37132.1. X56502 mRNA. Translation: CAA39857.1. X80685 Genomic DNA. Translation: CAA56700.1. AF109719 Genomic DNA. Translation: AAF03911.1. AK002903 mRNA. Translation: BAB22445.1. AK010730 mRNA. Translation: BAB27147.1. AK012369 mRNA. Translation: BAB28193.1. AK146186 mRNA. Translation: BAE26962.1. AK161330 mRNA. Translation: BAE36328.1. BC003775 mRNA. Translation: AAH03775.1.
IPI	IPI00126762.
PIR	S14724.
RefSeq	NP_034105.1. NM_009975.2.
UniGene	Mm.378901.
3D structure databases
ProteinModelPortal	P67871.
ModBase	Search...
Protein-protein interaction databases
IntAct	P67871. 7 interactions.
MINT	MINT-4153257.
PTM databases
PhosphoSite	P67871.
Proteomic databases
PaxDb	P67871.
PRIDE	P67871.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025246; ENSMUSP00000025246; ENSMUSG00000024387. ENSMUST00000173114; ENSMUSP00000134218; ENSMUSG00000024387.
GeneID	13001.
KEGG	mmu:13001.
UCSC	uc008cfu.1. mouse.
Organism-specific databases
CTD	1460.
MGI	MGI:88548. Csnk2b.
Phylogenomic databases
eggNOG	COG5041.
GeneTree	ENSGT00390000003781.
HOGENOM	HOG000039270.
HOVERGEN	HBG051131.
InParanoid	P67871.
KO	K03115.
OrthoDB	EOG4FBHTQ.
Gene expression databases
ArrayExpress	P67871.
Bgee	P67871.
CleanEx	MM_CSNK2B.
Genevestigator	P67871.
GermOnline	ENSMUSG00000024387. Mus musculus.
Family and domain databases
Gene3D	1.10.1820.10. 1 hit. 2.20.25.20. 1 hit.
InterPro	IPR016149. Casein_kin_II_reg-sub_a-hlx. IPR016150. Casein_kin_II_reg-sub_b-sht. IPR000704. Casein_kinase_II_reg-sub. [Graphical view]
PANTHER	PTHR11740. PTHR11740. 1 hit.
Pfam	PF01214. CK_II_beta. 1 hit. [Graphical view]
PRINTS	PR00472. CASNKINASEII.
SMART	SM01085. CK_II_beta. 1 hit. [Graphical view]
SUPFAM	SSF57798. Casein_kinase_II_reg-sub. 1 hit.
PROSITE	PS01101. CK2_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P67871.
ChEMBL	CHEMBL4095.
NextBio	282816.
SOURCE	Search...

Entry information

Entry name

CSK2B_MOUSE

Accession

Primary (citable) accession number: P67871
Secondary accession number(s): P07312, P13862, Q3TTJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 29, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program