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Protein

Prohibitin

Gene

Phb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging (By similarity).By similarity

GO - Molecular functioni

  1. enzyme binding Source: MGI
  2. histone deacetylase binding Source: MGI
  3. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: MGI
  4. transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  1. cellular response to interleukin-6 Source: MGI
  2. DNA biosynthetic process Source: MGI
  3. histone deacetylation Source: MGI
  4. negative regulation of androgen receptor signaling pathway Source: MGI
  5. negative regulation of apoptotic process Source: Ensembl
  6. negative regulation of cell growth Source: MGI
  7. negative regulation of cell proliferation Source: MGI
  8. negative regulation of glucocorticoid receptor signaling pathway Source: MGI
  9. negative regulation of transcription, DNA-templated Source: MGI
  10. negative regulation of transcription by competitive promoter binding Source: MGI
  11. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  12. organ regeneration Source: Ensembl
  13. osteoblast differentiation Source: MGI
  14. ovarian follicle atresia Source: Ensembl
  15. ovarian follicle development Source: Ensembl
  16. positive regulation of transcription, DNA-templated Source: MGI
  17. progesterone receptor signaling pathway Source: MGI
  18. regulation of transcription, DNA-templated Source: MGI
  19. response to drug Source: Ensembl
  20. response to ethanol Source: Ensembl
  21. response to immobilization stress Source: Ensembl
  22. response to peptide hormone Source: Ensembl
  23. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Biological processi

DNA synthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Prohibitin
Alternative name(s):
B-cell receptor-associated protein 32
Short name:
BAP 32
Gene namesi
Name:Phb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:97572. Phb.

Subcellular locationi

Mitochondrion inner membrane 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular vesicular exosome Source: MGI
  3. extrinsic component of mitochondrial outer membrane Source: Ensembl
  4. integral component of plasma membrane Source: MGI
  5. membrane Source: MGI
  6. mitochondrial crista Source: Ensembl
  7. mitochondrial inner membrane Source: MGI
  8. mitochondrion Source: MGI
  9. myelin sheath Source: UniProtKB
  10. nucleoplasm Source: MGI
  11. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272ProhibitinPRO_0000213879Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei91 – 911PhosphothreonineBy similarity
Modified residuei128 – 1281N6-acetyllysine1 Publication
Modified residuei186 – 1861N6-acetyllysine1 Publication
Modified residuei202 – 2021N6-acetyllysine; alternate1 Publication
Modified residuei202 – 2021N6-succinyllysine; alternate1 Publication
Modified residuei249 – 2491Phosphotyrosine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP67778.
PaxDbiP67778.
PRIDEiP67778.

2D gel databases

REPRODUCTION-2DPAGEIPI00133440.
P67778.
SWISS-2DPAGEP67778.

PTM databases

PhosphoSiteiP67778.

Expressioni

Tissue specificityi

Widely expressed in different tissues.1 Publication

Developmental stagei

Throughout gestation, highly expressed in brown fat, heart, liver, developing renal tubules and neurons, and detected at lower levels in tissues such as lung and exocrine pancreas.1 Publication

Gene expression databases

BgeeiP67778.
CleanExiMM_PHB.
ExpressionAtlasiP67778. baseline and differential.
GenevestigatoriP67778.

Interactioni

Subunit structurei

Interacts with PHB2. Interacts with STOML2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi202141. 2 interactions.
IntActiP67778. 9 interactions.
MINTiMINT-1841697.

Structurei

3D structure databases

ProteinModelPortaliP67778.
SMRiP67778. Positions 60-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili177 – 21135Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the prohibitin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0330.
GeneTreeiENSGT00550000074770.
HOGENOMiHOG000205692.
HOVERGENiHBG004457.
InParanoidiP67778.
KOiK17080.
OMAiNDIKDAD.
OrthoDBiEOG7V4B04.
TreeFamiTF300095.

Family and domain databases

InterProiIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00679. PROHIBITIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P67778-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV
60 70 80 90 100
GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA
110 120 130 140 150
SQLPRIYTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV
160 170 180 190 200
SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV
210 220 230 240 250
EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ
260 270
LSRSRNITYL PAGQSVLLQL PQ
Length:272
Mass (Da):29,820
Last modified:October 11, 2004 - v1
Checksum:i6B26073E169C2FFC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti255 – 2551R → W in BAE29988 (PubMed:16141072).Curated
Sequence conflicti255 – 2551R → W in BAE30089 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78682 mRNA. Translation: CAA55349.1.
AK002714 mRNA. Translation: BAB22305.1.
AK010619 mRNA. Translation: BAB27067.1.
AK150956 mRNA. Translation: BAE29988.1.
AK151073 mRNA. Translation: BAE30089.1.
AK168248 mRNA. Translation: BAE40198.1.
AK168656 mRNA. Translation: BAE40512.1.
AL732490 Genomic DNA. Translation: CAI24279.1.
BC083354 mRNA. Translation: AAH83354.1.
BC089034 mRNA. Translation: AAH89034.1.
CCDSiCCDS25280.1.
RefSeqiNP_032857.1. NM_008831.4.
UniGeneiMm.263862.

Genome annotation databases

EnsembliENSMUST00000125172; ENSMUSP00000119603; ENSMUSG00000038845.
GeneIDi18673.
KEGGimmu:18673.
UCSCiuc007lan.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78682 mRNA. Translation: CAA55349.1.
AK002714 mRNA. Translation: BAB22305.1.
AK010619 mRNA. Translation: BAB27067.1.
AK150956 mRNA. Translation: BAE29988.1.
AK151073 mRNA. Translation: BAE30089.1.
AK168248 mRNA. Translation: BAE40198.1.
AK168656 mRNA. Translation: BAE40512.1.
AL732490 Genomic DNA. Translation: CAI24279.1.
BC083354 mRNA. Translation: AAH83354.1.
BC089034 mRNA. Translation: AAH89034.1.
CCDSiCCDS25280.1.
RefSeqiNP_032857.1. NM_008831.4.
UniGeneiMm.263862.

3D structure databases

ProteinModelPortaliP67778.
SMRiP67778. Positions 60-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202141. 2 interactions.
IntActiP67778. 9 interactions.
MINTiMINT-1841697.

PTM databases

PhosphoSiteiP67778.

2D gel databases

REPRODUCTION-2DPAGEIPI00133440.
P67778.
SWISS-2DPAGEP67778.

Proteomic databases

MaxQBiP67778.
PaxDbiP67778.
PRIDEiP67778.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000125172; ENSMUSP00000119603; ENSMUSG00000038845.
GeneIDi18673.
KEGGimmu:18673.
UCSCiuc007lan.2. mouse.

Organism-specific databases

CTDi5245.
MGIiMGI:97572. Phb.

Phylogenomic databases

eggNOGiCOG0330.
GeneTreeiENSGT00550000074770.
HOGENOMiHOG000205692.
HOVERGENiHBG004457.
InParanoidiP67778.
KOiK17080.
OMAiNDIKDAD.
OrthoDBiEOG7V4B04.
TreeFamiTF300095.

Miscellaneous databases

NextBioi294690.
PROiP67778.
SOURCEiSearch...

Gene expression databases

BgeeiP67778.
CleanExiMM_PHB.
ExpressionAtlasiP67778. baseline and differential.
GenevestigatoriP67778.

Family and domain databases

InterProiIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00679. PROHIBITIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The IgM antigen receptor of B lymphocytes is associated with prohibitin and a prohibitin-related protein."
    Terashima M., Kim K.-M., Adachi T., Nielsen P.J., Reth M., Koehler G., Lamers M.C.
    EMBO J. 13:3782-3792(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Lymphoid tissue.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and DBA/2.
    Tissue: Bone marrow, Heart and Kidney.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Eye.
  5. Lubec G., Klug S.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 12-34; 94-105; 158-177; 220-239 AND 241-253, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hippocampus.
  6. "Mammalian prohibitin proteins respond to mitochondrial stress and decrease during cellular senescence."
    Coates P.J., Nenutil R., McGregor A., Picksley S.M., Crouch D.H., Hall P.A., Wright E.G.
    Exp. Cell Res. 265:262-273(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PHB2, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-249, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-202, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast and Liver.
  9. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-128; LYS-186 AND LYS-202, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPHB_MOUSE
AccessioniPrimary (citable) accession number: P67778
Secondary accession number(s): P24142, Q3UB75, Q5SQG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 4, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.