P67778 (PHB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prohibitin

Alternative name(s):
B-cell receptor-associated protein 32
Short name=BAP 32

Gene names

Name:

Phb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging By similarity.
Subunit structure	Interacts with PHB2. Interacts with STOML2 By similarity. Ref.6
Subcellular location	Mitochondrion inner membrane Ref.6.
Tissue specificity	Widely expressed in different tissues. Ref.1
Developmental stage	Throughout gestation, highly expressed in brown fat, heart, liver, developing renal tubules and neurons, and detected at lower levels in tissues such as lung and exocrine pancreas. Ref.6
Sequence similarities	Belongs to the prohibitin family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Coiled coil
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA biosynthetic process Inferred from mutant phenotype PubMed 18480465. Source: MGI DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cellular response to interleukin-6 Inferred from electronic annotation. Source: Compara histone deacetylation Inferred from electronic annotation. Source: Compara negative regulation of androgen receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of cell growth Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of glucocorticoid receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of transcription by competitive promoter binding Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 18480465. Source: MGI organ regeneration Inferred from electronic annotation. Source: Compara ovarian follicle atresia Inferred from electronic annotation. Source: Compara ovarian follicle development Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara progesterone receptor signaling pathway Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to peptide hormone stimulus Inferred from electronic annotation. Source: Compara response to stress Inferred from electronic annotation. Source: Compara
Cellular_component	extrinsic to mitochondrial outer membrane Inferred from electronic annotation. Source: Compara integral to plasma membrane Inferred from electronic annotation. Source: Compara mitochondrial crista Inferred from electronic annotation. Source: Compara mitochondrial inner membrane Inferred from direct assay PubMed 12865426. Source: MGI nucleoplasm Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 18480465. Source: MGI
Molecular_function	sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from direct assay PubMed 18480465. Source: MGI transcription regulatory region DNA binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Prohibitin		PRO_0000213879
Regions
	Coiled coil	177 – 211	35	Potential
Amino acid modifications
	Modified residue	202	1	N6-acetyllysine By similarity
	Modified residue	249	1	Phosphotyrosine Ref.7
Experimental info
	Sequence conflict	255	1	R → W in BAE29988. Ref.2
	Sequence conflict	255	1	R → W in BAE30089. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P67778 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6B26073E169C2FFC	FASTA	272	29,820
	10 20 30 40 50 60 MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV GEGTHFLIPW 70 80 90 100 110 120 VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA SQLPRIYTSI GEDYDERVLP 130 140 150 160 170 180 SITTEILKSV VARFDAGELI TQRELVSRQV SDDLTERAAT FGLILDDVSL THLTFGKEFT 190 200 210 220 230 240 EAVEAKQVAQ QEAERARFVV EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK 250 260 270 LEAAEDIAYQ LSRSRNITYL PAGQSVLLQL PQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The IgM antigen receptor of B lymphocytes is associated with prohibitin and a prohibitin-related protein." Terashima M., Kim K.-M., Adachi T., Nielsen P.J., Reth M., Koehler G., Lamers M.C. EMBO J. 13:3782-3792(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Lymphoid tissue.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and DBA/2. Tissue: Bone marrow, Heart and Kidney.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Eye.
[5]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 12-34; 94-105; 158-177; 220-239 AND 241-253, MASS SPECTROMETRY. Tissue: Hippocampus.
[6]	"Mammalian prohibitin proteins respond to mitochondrial stress and decrease during cellular senescence." Coates P.J., Nenutil R., McGregor A., Picksley S.M., Crouch D.H., Hall P.A., Wright E.G. Exp. Cell Res. 265:262-273(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PHB2, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[7]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-249, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78682 mRNA. Translation: CAA55349.1. AK002714 mRNA. Translation: BAB22305.1. AK010619 mRNA. Translation: BAB27067.1. AK150956 mRNA. Translation: BAE29988.1. AK151073 mRNA. Translation: BAE30089.1. AK168248 mRNA. Translation: BAE40198.1. AK168656 mRNA. Translation: BAE40512.1. AL732490 Genomic DNA. Translation: CAI24279.1. BC083354 mRNA. Translation: AAH83354.1. BC089034 mRNA. Translation: AAH89034.1.
IPI	IPI00133440.
RefSeq	NP_032857.1. NM_008831.4.
UniGene	Mm.263862.
3D structure databases
ProteinModelPortal	P67778.
SMR	P67778. Positions 60-194.
ModBase	Search...
Protein-protein interaction databases
IntAct	P67778. 6 interactions.
PTM databases
PhosphoSite	P67778.
2D gel databases
REPRODUCTION-2DPAGE	IPI00133440. P67778.
SWISS-2DPAGE	P67778.
Proteomic databases
PaxDb	P67778.
PRIDE	P67778.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000125172; ENSMUSP00000119603; ENSMUSG00000038845.
GeneID	18673.
KEGG	mmu:18673.
Organism-specific databases
CTD	5245.
MGI	MGI:97572. Phb.
Phylogenomic databases
eggNOG	COG0330.
GeneTree	ENSGT00550000074770.
HOGENOM	HOG000205692.
HOVERGEN	HBG004457.
InParanoid	P67778.
OMA	IIFDVRS.
OrthoDB	EOG4X3H23.
Gene expression databases
ArrayExpress	P67778.
Bgee	P67778.
CleanEx	MM_PHB.
Genevestigator	P67778.
GermOnline	ENSMUSG00000038845. Mus musculus.
Family and domain databases
InterPro	IPR001107. Band_7. IPR000163. Prohibitin. [Graphical view]
PANTHER	PTHR23222. PTHR23222. 1 hit.
Pfam	PF01145. Band_7. 1 hit. [Graphical view]
PRINTS	PR00679. PROHIBITIN.
SMART	SM00244. PHB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294690.
SOURCE	Search...

Entry information

Entry name

PHB_MOUSE

Accession

Primary (citable) accession number: P67778
Secondary accession number(s): P24142, Q3UB75, Q5SQG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program