P67777 (PP2AA_RABIT) Reviewed, UniProtKB/Swiss-Prot
Last modified July 9, 2014. Version 74. History...
Names and origin
|Protein names||Recommended name:|
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
|Organism||Oryctolagus cuniculus (Rabbit) [Reference proteome]|
|Taxonomic identifier||9986 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus|
|Sequence length||309 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Activates RAF1 by dephosphorylating it at 'Ser-259' By similarity.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.
Binds 2 manganese ions per subunit By similarity.
PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with NXN; the interaction is direct. Interacts with TP53, SGOL1 and SGOL2. Interacts with AXIN1; the interaction dephosphorylates AXIN1 By similarity. Interacts with PIM3; this interaction promotes dephosphorylation, ubiquitination and proteasomal degradation of PIM3 By similarity. Interacts with RAF1. Interacts with GSK3B (via C2 domain) By similarity. Interaction with IGBP1 protects unassembled PPP2CA from degradative ubiquitination By similarity.
Cytoplasm. Nucleus By similarity. Chromosome › centromere By similarity. Cytoplasm › cytoskeleton › spindle pole By similarity. Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 By similarity.
Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization By similarity.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation By similarity.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 309||309||Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform||PRO_0000058842|
|Active site||118||1||Proton donor By similarity|
|Metal binding||57||1||Manganese 1 By similarity|
|Metal binding||59||1||Manganese 1 By similarity|
|Metal binding||85||1||Manganese 1 By similarity|
|Metal binding||85||1||Manganese 2 By similarity|
|Metal binding||117||1||Manganese 2 By similarity|
|Metal binding||167||1||Manganese 2 By similarity|
|Metal binding||241||1||Manganese 2 By similarity|
Amino acid modifications
|Modified residue||307||1||Phosphotyrosine By similarity|
|Modified residue||309||1||Leucine methyl ester By similarity|
|||"Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a type-2A protein phosphatase."|
da Cruz e Silva O.B., Alemany S., Campbell D.G., Cohen P.T.W.
FEBS Lett. 221:415-422(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skeletal muscle.
|X06087 mRNA. Translation: CAA29471.1.|
|PIR||PARBA1. S00104. |
|RefSeq||NP_001095156.1. NM_001101686.1. |
3D structure databases
|SMR||P67777. Positions 2-296. |
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSOCUT00000015956; ENSOCUP00000013713; ENSOCUG00000015961. |
Family and domain databases
|Gene3D||188.8.131.52. 1 hit. |
|InterPro||IPR004843. Calcineurin-like_PHP_apaH. |
|Pfam||PF00149. Metallophos. 1 hit. |
|PRINTS||PR00114. STPHPHTASE. |
|SMART||SM00156. PP2Ac. 1 hit. |
|SUPFAM||SSF56300. SSF56300. 1 hit. |
|PROSITE||PS00125. SER_THR_PHOSPHATASE. 1 hit. |
|Accession||Primary (citable) accession number: P67777|
Secondary accession number(s): P05323, P13197
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families