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Protein

Antitoxin HigA

Gene

higA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Antitoxin component of a type II toxin-antitoxin (TA) system. Functions as an mRNA interferase antitoxin; overexpression prevents HigB-mediated cessation of cell growth and inhibition of cell proliferation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi95 – 114H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processToxin-antitoxin system, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G7601-MONOMER
MetaCyc:G7601-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin HigA
Gene namesi
Name:higA
Synonyms:ygjM
Ordered Locus Names:b3082, JW3053
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12724 higA

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001497631 – 138Antitoxin HigAAdd BLAST138

Proteomic databases

PaxDbiP67701
PRIDEiP67701

Expressioni

Inductioni

Induced by amino acid starvation and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the HigA antitoxin. Transcription is negatively regulated by the cognate locus, probably by this protein. A member of the higB-higA operon.1 Publication

Interactioni

Subunit structurei

Probably forms a complex with the mRNA interferase HigB which inhibits the mRNA interferase activity.Curated

Protein-protein interaction databases

BioGridi4262401, 7 interactors
IntActiP67701, 13 interactors
STRINGi316385.ECDH10B_3257

Structurei

Secondary structure

1138
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 17Combined sources12
Helixi19 – 22Combined sources4
Helixi27 – 43Combined sources17
Helixi48 – 63Combined sources16
Helixi65 – 67Combined sources3
Helixi68 – 76Combined sources9
Helixi81 – 91Combined sources11
Helixi107 – 110Combined sources4
Turni111 – 113Combined sources3
Beta strandi123 – 125Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IFGX-ray2.70B/D1-138[»]
ProteinModelPortaliP67701
SMRiP67701
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 136HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST53

Sequence similaritiesi

Belongs to the HigA antitoxin family.Curated

Phylogenomic databases

eggNOGiENOG4108Z3N Bacteria
COG5499 LUCA
HOGENOMiHOG000026115
KOiK18831
OMAiPENEADY

Family and domain databases

CDDicd00093 HTH_XRE, 1 hit
InterProiView protein in InterPro
IPR001387 Cro/C1-type_HTH
IPR010982 Lambda_DNA-bd_dom_sf
PfamiView protein in Pfam
PF01381 HTH_3, 1 hit
SMARTiView protein in SMART
SM00530 HTH_XRE, 1 hit
SUPFAMiSSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS50943 HTH_CROC1, 1 hit

Sequencei

Sequence statusi: Complete.

P67701-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAIADILQA GEKLTAVAPF LAGIQNEEQY TQALELVDHL LLNDPENPLL
60 70 80 90 100
DLVCAKITAW EESAPEFAEF NAMAQAMPGG IAVIRTLMDQ YGLTLSDLPE
110 120 130
IGSKSMVSRV LSGKRKLTLE HAKKLATRFG ISPALFID
Length:138
Mass (Da):14,995
Last modified:October 11, 2004 - v1
Checksum:i4A14D9E43480DCB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA57883.1
U00096 Genomic DNA Translation: AAC76117.1
AP009048 Genomic DNA Translation: BAE77132.1
PIRiG65096
RefSeqiNP_417553.1, NC_000913.3
WP_000560266.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76117; AAC76117; b3082
BAE77132; BAE77132; BAE77132
GeneIDi947593
KEGGiecj:JW3053
eco:b3082
PATRICifig|1411691.4.peg.3647

Similar proteinsi

Entry informationi

Entry nameiHIGA_ECOLI
AccessioniPrimary (citable) accession number: P67701
Secondary accession number(s): P42594, Q2M9C4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health