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Protein

Antitoxin HigA

Gene

higA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Antitoxin component of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin; overexpression prevents HigB-mediated cessation of cell growth and inhibition of cell proliferation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi95 – 114H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G7601-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin HigA
Gene namesi
Name:higA
Synonyms:ygjM
Ordered Locus Names:b3082, JW3053
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12724. higA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001497631 – 138Antitoxin HigAAdd BLAST138

Proteomic databases

PaxDbiP67701.
PRIDEiP67701.

Expressioni

Inductioni

Induced by amino acid starvation and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the HigA antitoxin. Transcription is negatively regulated by the cognate locus, probably by this protein. A member of the higB-higA operon.1 Publication

Interactioni

Subunit structurei

Probably forms a complex with the mRNA interferase HigB which inhibits the mRNA interferase activity.Curated

Protein-protein interaction databases

BioGridi4262401. 7 interactors.
IntActiP67701. 13 interactors.
STRINGi511145.b3082.

Structurei

Secondary structure

1138
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 17Combined sources12
Helixi19 – 22Combined sources4
Helixi27 – 43Combined sources17
Helixi48 – 63Combined sources16
Helixi65 – 67Combined sources3
Helixi68 – 76Combined sources9
Helixi81 – 91Combined sources11
Helixi107 – 110Combined sources4
Turni111 – 113Combined sources3
Beta strandi123 – 125Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IFGX-ray2.70B/D1-138[»]
ProteinModelPortaliP67701.
SMRiP67701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 136HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST53

Sequence similaritiesi

Belongs to the HigA antitoxin family.Curated

Phylogenomic databases

eggNOGiENOG4108Z3N. Bacteria.
COG5499. LUCA.
HOGENOMiHOG000026115.
KOiK18831.
OMAiLACAKIT.

Family and domain databases

InterProiView protein in InterPro
IPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
PfamiView protein in Pfam
PF01381. HTH_3. 1 hit.
SMARTiView protein in SMART
SM00530. HTH_XRE. 1 hit.
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiView protein in PROSITE
PS50943. HTH_CROC1. 1 hit.

Sequencei

Sequence statusi: Complete.

P67701-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAIADILQA GEKLTAVAPF LAGIQNEEQY TQALELVDHL LLNDPENPLL
60 70 80 90 100
DLVCAKITAW EESAPEFAEF NAMAQAMPGG IAVIRTLMDQ YGLTLSDLPE
110 120 130
IGSKSMVSRV LSGKRKLTLE HAKKLATRFG ISPALFID
Length:138
Mass (Da):14,995
Last modified:October 11, 2004 - v1
Checksum:i4A14D9E43480DCB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57883.1.
U00096 Genomic DNA. Translation: AAC76117.1.
AP009048 Genomic DNA. Translation: BAE77132.1.
PIRiG65096.
RefSeqiNP_417553.1. NC_000913.3.
WP_000560266.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76117; AAC76117; b3082.
BAE77132; BAE77132; BAE77132.
GeneIDi947593.
KEGGiecj:JW3053.
eco:b3082.
PATRICi32121582. VBIEscCol129921_3177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57883.1.
U00096 Genomic DNA. Translation: AAC76117.1.
AP009048 Genomic DNA. Translation: BAE77132.1.
PIRiG65096.
RefSeqiNP_417553.1. NC_000913.3.
WP_000560266.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IFGX-ray2.70B/D1-138[»]
ProteinModelPortaliP67701.
SMRiP67701.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262401. 7 interactors.
IntActiP67701. 13 interactors.
STRINGi511145.b3082.

Proteomic databases

PaxDbiP67701.
PRIDEiP67701.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76117; AAC76117; b3082.
BAE77132; BAE77132; BAE77132.
GeneIDi947593.
KEGGiecj:JW3053.
eco:b3082.
PATRICi32121582. VBIEscCol129921_3177.

Organism-specific databases

EchoBASEiEB2583.
EcoGeneiEG12724. higA.

Phylogenomic databases

eggNOGiENOG4108Z3N. Bacteria.
COG5499. LUCA.
HOGENOMiHOG000026115.
KOiK18831.
OMAiLACAKIT.

Enzyme and pathway databases

BioCyciEcoCyc:G7601-MONOMER.

Miscellaneous databases

PROiPR:P67701.

Family and domain databases

InterProiView protein in InterPro
IPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
PfamiView protein in Pfam
PF01381. HTH_3. 1 hit.
SMARTiView protein in SMART
SM00530. HTH_XRE. 1 hit.
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiView protein in PROSITE
PS50943. HTH_CROC1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHIGA_ECOLI
AccessioniPrimary (citable) accession number: P67701
Secondary accession number(s): P42594, Q2M9C4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: May 10, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.