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Protein

Antitoxin HicB

Gene

hicB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Antitoxin component of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin; overexpression prevents HicA-mediated cessation of cell growth and inhibition of cell proliferation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi93 – 112H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6749-MONOMER.
ECOL316407:JW1433-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin HicB
Gene namesi
Name:hicB
Synonyms:ydcQ
Ordered Locus Names:b1438, JW1433
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13760. hicB.

Pathology & Biotechi

Disruption phenotypei

Disruption suppresses an rpoE disruption; in a wild-type background disruption down-regulates extracytoplasmic stress responses and outer membrane vesicle production.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001497571 – 138Antitoxin HicBAdd BLAST138

Post-translational modificationi

May be degraded by Lon protease.By similarity

Proteomic databases

PaxDbiP67697.
PRIDEiP67697.

Expressioni

Inductioni

Induced by amino acid starvation, carbon starvation and when translation is blocked. Induction no longer occurs in the absence of Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the HicB antitoxin. A member of the hicA-hicB operon.1 Publication

Interactioni

Subunit structurei

Probably forms a complex with the mRNA interferase HicA; when the 2 dissociate the mRNA interferase becomes active.By similarity

Protein-protein interaction databases

BioGridi4259496. 3 interactors.
DIPiDIP-48269N.
IntActiP67697. 1 interactor.
STRINGi511145.b1438.

Structurei

3D structure databases

ProteinModelPortaliP67697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 136HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST55

Sequence similaritiesi

Belongs to the HicB antitoxin family.Curated
Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105PF0. Bacteria.
COG1598. LUCA.
HOGENOMiHOG000219259.
KOiK18843.
OMAiAMEFYFE.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR031807. HicB-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF15919. HicB_lk_antitox. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P67697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYPVTLTPA PEGGYMVSFV DIPEALTQGE TVAEAMEAAK DALLTAFDFY
60 70 80 90 100
FEDNELIPLP SPLNSHDHFI EVPLSVASKV LLLNAFLQSE ITQQELARRI
110 120 130
GKPKQEITRL FNLHHATKID AVQLAAKALG KELSLVMV
Length:138
Mass (Da):15,247
Last modified:June 26, 2007 - v2
Checksum:iC7B6C1F0D0A8BE26
GO

Sequence cautioni

The sequence BAE76439 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74520.2.
AP009048 Genomic DNA. Translation: BAE76439.1. Different initiation.
PIRiA64896.
RefSeqiNP_415955.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74520; AAC74520; b1438.
BAE76439; BAE76439; BAE76439.
GeneIDi946001.
KEGGiecj:JW1433.
eco:b1438.
PATRICi32118166. VBIEscCol129921_1503.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74520.2.
AP009048 Genomic DNA. Translation: BAE76439.1. Different initiation.
PIRiA64896.
RefSeqiNP_415955.2. NC_000913.3.

3D structure databases

ProteinModelPortaliP67697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259496. 3 interactors.
DIPiDIP-48269N.
IntActiP67697. 1 interactor.
STRINGi511145.b1438.

Proteomic databases

PaxDbiP67697.
PRIDEiP67697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74520; AAC74520; b1438.
BAE76439; BAE76439; BAE76439.
GeneIDi946001.
KEGGiecj:JW1433.
eco:b1438.
PATRICi32118166. VBIEscCol129921_1503.

Organism-specific databases

EchoBASEiEB3523.
EcoGeneiEG13760. hicB.

Phylogenomic databases

eggNOGiENOG4105PF0. Bacteria.
COG1598. LUCA.
HOGENOMiHOG000219259.
KOiK18843.
OMAiAMEFYFE.

Enzyme and pathway databases

BioCyciEcoCyc:G6749-MONOMER.
ECOL316407:JW1433-MONOMER.

Miscellaneous databases

PROiP67697.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR031807. HicB-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF15919. HicB_lk_antitox. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHICB_ECOLI
AccessioniPrimary (citable) accession number: P67697
Secondary accession number(s): P76107, Q2MBB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 26, 2007
Last modified: November 2, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.