P67697 (HICB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Antitoxin HicB

Gene names

Name:	hicB
Synonyms:	ydcQ
Ordered Locus Names:	b1438, JW1433

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Antitoxin component of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin; overexpression prevents HicA-mediated cessation of cell growth and inhibition of cell proliferation. Ref.3 Ref.5
Subunit structure	Probably forms a complex with the mRNA interferase HicA; when the 2 dissociate the mRNA interferase becomes active By similarity.
Induction	Induced by amino acid starvation, carbon starvation and when translation is blocked. Induction no longer occurs in the absence of Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the HicB antitoxin. A member of the hicA-hicB operon. Ref.5
Post-translational modification	May be degraded by Lon protease By similarity.
Disruption phenotype	Disruption suppresses an rpoE disruption; in a wild-type background disruption down-regulates extracytoplasmic stress responses and outer membrane vesicle production. Ref.4
Sequence similarities	Belongs to the HicB antitoxin family. Contains 1 HTH cro/C1-type DNA-binding domain.
Sequence caution	The sequence BAE76439.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Stress response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from genetic interaction Ref.4. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Antitoxin HicB		PRO_0000149757
Regions
	Domain	82 – 136	55	HTH cro/C1-type
	DNA binding	93 – 112	20	H-T-H motif Potential

Sequences

	Sequence		Length	Mass (Da)
	P67697 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: C7B6C1F0D0A8BE26	FASTA	138	15,247
	10 20 30 40 50 60 MRYPVTLTPA PEGGYMVSFV DIPEALTQGE TVAEAMEAAK DALLTAFDFY FEDNELIPLP 70 80 90 100 110 120 SPLNSHDHFI EVPLSVASKV LLLNAFLQSE ITQQELARRI GKPKQEITRL FNLHHATKID 130 AVQLAAKALG KELSLVMV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The HicAB cassette, a putative novel, RNA-targeting toxin-antitoxin system in archaea and bacteria." Makarova K.S., Grishin N.V., Koonin E.V. Bioinformatics 22:2581-2584(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PREDICTION OF FUNCTION.
[4]	"A suppressor of cell death caused by the loss of sigmaE downregulates extracytoplasmic stress responses and outer membrane vesicle production in Escherichia coli." Button J.E., Silhavy T.J., Ruiz N. J. Bacteriol. 189:1523-1530(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUPPRESSION OF RPOE ESSENTIALITY, DISRUPTION PHENOTYPE. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[5]	"HicA of Escherichia coli defines a novel family of translation-independent mRNA interferases in bacteria and archaea." Jorgensen M.G., Pandey D.P., Jaskolska M., Gerdes K. J. Bacteriol. 191:1191-1199(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS AN MRNA INTERFERASE ANTITOXIN, INDUCTION, OPERON STRUCTURE. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74520.2. AP009048 Genomic DNA. Translation: BAE76439.1. Different initiation.
PIR	A64896.
RefSeq	NP_415955.2. NC_000913.2. YP_489703.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P67697.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48269N.
STRING	511145.b1438.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74520; AAC74520; b1438. BAE76439; BAE76439; BAE76439.
GeneID	12930502. 946001.
KEGG	ecj:Y75_p1414. eco:b1438.
PATRIC	32118166. VBIEscCol129921_1503.
Organism-specific databases
EchoBASE	EB3523.
EcoGene	EG13760. hicB.
Phylogenomic databases
eggNOG	COG1598.
HOGENOM	HOG000219259.
OMA	DIAIHVP.
ProtClustDB	CLSK880069.
Enzyme and pathway databases
BioCyc	EcoCyc:G6749-MONOMER. ECOL316407:JW1433-MONOMER.
Gene expression databases
Genevestigator	P67697.
Family and domain databases
Gene3D	1.10.260.40. 1 hit.
InterPro	IPR001387. HTH. IPR010982. Lambda_DNA-bd_dom. IPR005357. UPF0150. [Graphical view]
Pfam	PF01381. HTH_3. 1 hit. PF03681. UPF0150. 1 hit. [Graphical view]
SMART	SM00530. HTH_XRE. 1 hit. [Graphical view]
PROSITE	PS50943. HTH_CROC1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HICB_ECOLI

Accession

Primary (citable) accession number: P67697
Secondary accession number(s): P76107, Q2MBB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	June 26, 2007
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program