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Protein

Antitoxin HicB

Gene

hicB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Antitoxin component of a type II toxin-antitoxin (TA) system. Functions as an mRNA interferase antitoxin; overexpression prevents HicA-mediated cessation of cell growth and inhibition of cell proliferation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi93 – 112H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processStress response, Toxin-antitoxin system, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G6749-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin HicB
Gene namesi
Name:hicB
Synonyms:ydcQ
Ordered Locus Names:b1438, JW1433
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13760 hicB

Pathology & Biotechi

Disruption phenotypei

Disruption suppresses an rpoE disruption; in a wild-type background disruption down-regulates extracytoplasmic stress responses and outer membrane vesicle production.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001497571 – 138Antitoxin HicBAdd BLAST138

Post-translational modificationi

May be degraded by Lon protease.By similarity

Proteomic databases

PaxDbiP67697
PRIDEiP67697

Expressioni

Inductioni

Induced by amino acid starvation, carbon starvation and when translation is blocked. Induction no longer occurs in the absence of Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the HicB antitoxin. A member of the hicA-hicB operon.1 Publication

Interactioni

Subunit structurei

Probably forms a complex with the mRNA interferase HicA; when the 2 dissociate the mRNA interferase becomes active.By similarity

Protein-protein interaction databases

BioGridi4259496, 3 interactors
DIPiDIP-48269N
IntActiP67697, 1 interactor
STRINGi316385.ECDH10B_1568

Structurei

3D structure databases

ProteinModelPortaliP67697
SMRiP67697
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 136HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST55

Sequence similaritiesi

Belongs to the HicB antitoxin family.Curated

Phylogenomic databases

eggNOGiENOG4105PF0 Bacteria
COG1598 LUCA
HOGENOMiHOG000219259
KOiK18843
OMAiAMEFYFE

Family and domain databases

CDDicd00093 HTH_XRE, 1 hit
InterProiView protein in InterPro
IPR001387 Cro/C1-type_HTH
IPR031807 HicB-like
IPR010982 Lambda_DNA-bd_dom_sf
IPR035069 TTHA1013/TTHA0281-like
PfamiView protein in Pfam
PF15919 HicB_lk_antitox, 1 hit
PF01381 HTH_3, 1 hit
SMARTiView protein in SMART
SM00530 HTH_XRE, 1 hit
SUPFAMiSSF143100 SSF143100, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS50943 HTH_CROC1, 1 hit

Sequencei

Sequence statusi: Complete.

P67697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYPVTLTPA PEGGYMVSFV DIPEALTQGE TVAEAMEAAK DALLTAFDFY
60 70 80 90 100
FEDNELIPLP SPLNSHDHFI EVPLSVASKV LLLNAFLQSE ITQQELARRI
110 120 130
GKPKQEITRL FNLHHATKID AVQLAAKALG KELSLVMV
Length:138
Mass (Da):15,247
Last modified:June 26, 2007 - v2
Checksum:iC7B6C1F0D0A8BE26
GO

Sequence cautioni

The sequence BAE76439 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74520.2
AP009048 Genomic DNA Translation: BAE76439.1 Different initiation.
PIRiA64896
RefSeqiNP_415955.2, NC_000913.3
WP_001270286.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74520; AAC74520; b1438
BAE76439; BAE76439; BAE76439
GeneIDi946001
KEGGiecj:JW1433
eco:b1438
PATRICifig|511145.12.peg.1503

Similar proteinsi

Entry informationi

Entry nameiHICB_ECOLI
AccessioniPrimary (citable) accession number: P67697
Secondary accession number(s): P76107, Q2MBB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 26, 2007
Last modified: March 28, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health