Reviewed, UniProtKB/Swiss-Prot P67657 (MTNN_MYCBO)

Names and origin

Protein names

Recommended name:
    MTA/SAH nucleosidase
    EC=3.2.2.9
Alternative name(s):
    5'-methylthioadenosine nucleosidase
    S-adenosylhomocysteine nucleosidase

Gene names

Name:	mtnN
Synonyms:	mtn
Ordered Locus Names:	Mb0094

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) By similarity.
Catalytic activity	S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. Methylthioadenosine + H2O = adenine + 5-methylthio-D-ribose.
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine salvage Inferred from electronic annotation. Source: InterPro nucleoside catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylhomocysteine nucleosidase activity Inferred from electronic annotation. Source: EC methylthioadenosine nucleosidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	MTA/SAH nucleosidase		PRO_0000164445

Sequences

	Sequence		Length	Mass (Da)
	P67657-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: EA80AA80B2BFEE79	FASTA	255	27,340
	10 20 30 40 50 60 MAVTVGVICA IPQELAYLRG VLVDAKRQQV AQILFDSGQL DAHRVVLAAA GMGKVNTGLT 70 80 90 100 110 120 ATLLADRFGC RTIVFTGVAG GLDPELCIGD IVIADRVVQH DFGLLTDERL RPYQPGHIPF 130 140 150 160 170 180 IEPTERLGYP VDPAVIDRVK HRLDGFTLAP LSTAAGGGGR QPRIYYGTIL TGDQYLHCER 190 200 210 220 230 240 TRNRLHHELG GMAVEMEGGA VAQICASFDI PWLVIRALSD LAGADSGVDF NRFVGEVAAS 250 SARVLLRLLP VLTAC

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248334 Genomic DNA. Translation: CAD92956.1.
RefSeq	NP_853762.1.
3D structure databases
HSSP	HSSP built from PDB template 1JYS based on UniProtKB P24247.
ModBase	Search...
Genome annotation databases
GeneID	1090778.
GenomeReviews	Gene locus Mb0094 in contig BX248333_GR.
KEGG	mbo:Mb0094.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P67657.
OMA	AQVCHQF.
Enzyme and pathway databases
BRENDA	3.2.2.9. 3091.
Family and domain databases
InterPro	IPR010049. MTA_SAH_Nsdase. IPR000845. Nucleoside_phosphorylase. IPR018017. Nucleoside_phosphorylase_1. [Graphical view]
PANTHER	PTHR21234. PNP_UDP. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNN_MYCBO

Accession

Primary (citable) accession number: P67657
Secondary accession number(s): Q10889

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)