P67656 (MTNN_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MTA/SAH nucleosidase
EC=3.2.2.9

Alternative name(s):
5'-methylthioadenosine nucleosidase
S-adenosylhomocysteine nucleosidase

Gene names

Name:	mtnN
Synonyms:	mtn
Ordered Locus Names:	Rv0091, MT0100
ORF Names:	MTCY251.10

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) By similarity.
Catalytic activity	S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. Methylthioadenosine + H2O = adenine + 5-methylthio-D-ribose.
Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro nucleoside catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylhomocysteine nucleosidase activity Inferred from electronic annotation. Source: EC methylthioadenosine nucleosidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	MTA/SAH nucleosidase		PRO_0000164446

Sequences

	Sequence		Length	Mass (Da)
	P67656 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: EA80AA80B2BFEE79	FASTA	255	27,340
	10 20 30 40 50 60 MAVTVGVICA IPQELAYLRG VLVDAKRQQV AQILFDSGQL DAHRVVLAAA GMGKVNTGLT 70 80 90 100 110 120 ATLLADRFGC RTIVFTGVAG GLDPELCIGD IVIADRVVQH DFGLLTDERL RPYQPGHIPF 130 140 150 160 170 180 IEPTERLGYP VDPAVIDRVK HRLDGFTLAP LSTAAGGGGR QPRIYYGTIL TGDQYLHCER 190 200 210 220 230 240 TRNRLHHELG GMAVEMEGGA VAQICASFDI PWLVIRALSD LAGADSGVDF NRFVGEVAAS 250 SARVLLRLLP VLTAC

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842572 Genomic DNA. Translation: CAA98927.1. AE000516 Genomic DNA. Translation: AAK44323.1.
PIR	C70750.
RefSeq	NP_214605.1. NC_000962.2. NP_334509.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P67656.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000003001; EBMYCP00000003001; EBMYCG00000002999. EBMYCT00000071187; EBMYCP00000069246; EBMYCG00000071182.
GeneID	886953. 922911.
GenomeReviews	Gene locus MT0100 in contig AE000516_GR. Gene locus Rv0091 in contig AL123456_GR.
KEGG	mtc:MT0100. mtu:Rv0091.
PATRIC	18121944. VBIMycTub22151_0106.
TIGR	MT0100.
Organism-specific databases
TubercuList	Rv0091.
Phylogenomic databases
GeneTree	EBGT00050000018357.
HOGENOM	HBG367723.
OMA	NVFWINT.
PhylomeDB	P67656.
ProtClustDB	CLSK790256.
Family and domain databases
InterPro	IPR010049. MTA_SAH_Nsdase. IPR018017. Nucleoside_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. [Graphical view]
KO	K01243.
PANTHER	PTHR21234. PNP_UDP. 1 hit. PTHR21234:SF6. PTHR21234:SF6. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTNN_MYCTU

Accession

Primary (citable) accession number: P67656
Secondary accession number(s): Q10889

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program