Reviewed, UniProtKB/Swiss-Prot P67654 (KDSC_SHIFL)

Names and origin

Protein names

Recommended name:
    3-deoxy-D-manno-octulosonate 8-phosphate phosphatase
    EC=3.1.3.45
Alternative name(s):
    KDO 8-P phosphatase

Gene names

Name:	kdsC
Ordered Locus Names:	SF3238, S3456

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of KDO 8-P to KDO and inorganic phosphate By similarity.
Catalytic activity	3-deoxy-D-manno-octulosonate 8-phosphate + H2O = 3-deoxy-D-manno-octulosonate + phosphate.
Cofactor	Magnesium Probable.
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 3/3. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the kdsC family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-manno-octulosonate-8-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	3-deoxy-D-manno-octulosonate 8-phosphate phosphatase		PRO_0000201701

Sequences

	Sequence		Length	Mass (Da)
	P67654-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6A40ED8C20B1E3A7	FASTA	188	20,015
	10 20 30 40 50 60 MSKAGASLAT CYGPVSADVM AKAENIRLLI LDVDGVLSDG LIYMGNNGEE LKAFNVRDGY 70 80 90 100 110 120 GIRCALTSDI EVAIITGRKA KLVEDRCATL GITHLYQGQS NKLIAFSDLL EKLAIAPENV 130 140 150 160 170 180 AYVGDDLIDW PVMEKVGLSV AVADAHPLLI PRADYVTRIA GGRGAVREVC DLLLLAQGKL DEAKGQSI

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN44704.1. AE014073 Genomic DNA. Translation: AAP18518.1.
RefSeq	NP_708997.1. NP_838707.1.
3D structure databases
HSSP	HSSP built from PDB template 1K1E based on UniProtKB P45314.
ModBase	Search...
Genome annotation databases
GeneID	1027121. 1079690.
GenomeReviews	Gene locus SF3238 in contig AE005674_GR. Gene locus S3456 in contig AE014073_GR.
KEGG	sfl:SF3238. sfx:S3456.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P67654.
OMA	P67654. KTFNTLD.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN44704.1-MON.
BRENDA	3.1.3.45. 189495.
Family and domain databases
InterPro	IPR013200. HAD-SF_hydro-like_3. IPR006549. HAD-SF_hydro_IIIA. IPR010023. Phosphatase_KdsC. [Graphical view]
Pfam	PF08282. Hydrolase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01662. HAD-SF-IIIA. 1 hit. TIGR01670. YrbI-phosphatas. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSC_SHIFL

Accession

Primary (citable) accession number: P67654
Secondary accession number(s): Q83JF3, Q8FD72

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)