P67654 (KDSC_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC
EC=3.1.3.45

Alternative name(s):
KDO 8-P phosphatase

Gene names

Name:	kdsC
Ordered Locus Names:	SF3238, S3456

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of lipopolysaccharides (LPSs). Catalyzes the hydrolysis of 3-deoxy-D-manno-octulosonate 8-phosphate (KDO 8-P) to 3-deoxy-D-manno-octulosonate (KDO) and inorganic phosphate By similarity.
Catalytic activity	3-deoxy-D-manno-octulosonate 8-phosphate + H2O = 3-deoxy-D-manno-octulosonate + phosphate.
Cofactor	Magnesium Probable.
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 3/3. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the kdsC family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-manno-octulosonate-8-phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC		PRO_0000201701
Regions
	Region	32 – 34	3	Substrate binding By similarity
	Region	55 – 59	5	Substrate binding By similarity
	Region	76 – 77	2	Substrate binding By similarity
Sites
	Metal binding	32	1	Magnesium By similarity
	Metal binding	34	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	125	1	Magnesium By similarity
	Binding site	34	1	Substrate By similarity
	Binding site	63	1	Substrate By similarity
	Binding site	78	1	Substrate By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	102	1	Substrate By similarity
	Binding site	187	1	Substrate; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67654 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6A40ED8C20B1E3A7	FASTA	188	20,015
	10 20 30 40 50 60 MSKAGASLAT CYGPVSADVM AKAENIRLLI LDVDGVLSDG LIYMGNNGEE LKAFNVRDGY 70 80 90 100 110 120 GIRCALTSDI EVAIITGRKA KLVEDRCATL GITHLYQGQS NKLIAFSDLL EKLAIAPENV 130 140 150 160 170 180 AYVGDDLIDW PVMEKVGLSV AVADAHPLLI PRADYVTRIA GGRGAVREVC DLLLLAQGKL DEAKGQSI

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN44704.1. AE014073 Genomic DNA. Translation: AAP18518.1.
RefSeq	NP_708997.1. NC_004337.2. NP_838707.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P67654.
SMR	P67654. Positions 8-188.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000085134; EBESCP00000081956; EBESCG00000084180. EBESCT00000089706; EBESCP00000086342; EBESCG00000088750.
GeneID	1027121. 1079690.
GenomeReviews	Gene locus SF3238 in contig AE005674_GR. Gene locus S3456 in contig AE014073_GR.
KEGG	sfl:SF3238. sfx:S3456.
PATRIC	18708663. VBIShiFle31049_3777.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009685.
HOGENOM	HBG471796.
OMA	KTFNTLD.
ProtClustDB	PRK09484.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN44704.1-MONOMER.
Family and domain databases
InterPro	IPR023214. HAD-like_dom. IPR013200. HAD-SF_hydro-like_3. IPR010023. KDO_8-P_phosphatase. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
KO	K03270.
PANTHER	PTHR26053. PTHR26053. 1 hit.
Pfam	PF08282. Hydrolase_3. 1 hit. [Graphical view]
PIRSF	PIRSF006118. KDO8-P_Ptase. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01670. YrbI-phosphatas. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSC_SHIFL

Accession

Primary (citable) accession number: P67654
Secondary accession number(s): Q83JF3, Q8FD72

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program