Reviewed, UniProtKB/Swiss-Prot P67653 (KDSC_ECOL6)

Names and origin

Protein names

Recommended name:
    3-deoxy-D-manno-octulosonate 8-phosphate phosphatase
    EC=3.1.3.45
Alternative name(s):
    KDO 8-P phosphatase

Gene names

Name:	kdsC
Ordered Locus Names:	c3958

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of KDO 8-P to KDO and inorganic phosphate By similarity.
Catalytic activity	3-deoxy-D-manno-octulosonate 8-phosphate + H2O = 3-deoxy-D-manno-octulosonate + phosphate.
Cofactor	Magnesium Probable.
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 3/3. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the kdsC family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-manno-octulosonate-8-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	3-deoxy-D-manno-octulosonate 8-phosphate phosphatase		PRO_0000201698

Sequences

	Sequence		Length	Mass (Da)
	P67653-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6A40ED8C20B1E3A7	FASTA	188	20,015
	10 20 30 40 50 60 MSKAGASLAT CYGPVSADVM AKAENIRLLI LDVDGVLSDG LIYMGNNGEE LKAFNVRDGY 70 80 90 100 110 120 GIRCALTSDI EVAIITGRKA KLVEDRCATL GITHLYQGQS NKLIAFSDLL EKLAIAPENV 130 140 150 160 170 180 AYVGDDLIDW PVMEKVGLSV AVADAHPLLI PRADYVTRIA GGRGAVREVC DLLLLAQGKL DEAKGQSI

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE014075 Genomic DNA. Translation: AAN82398.1.

RefSeq

NP_755824.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2R8E	X-ray	1.40	A/B/C/D/E/F/G/H	1-188	[»]
2R8X	X-ray	2.60	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P	1-188	[»]
2R8Y	X-ray	1.85	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P	1-188	[»]
2R8Z	X-ray	2.10	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P	1-188	[»]

ModBase

Search...

Genome annotation databases

GeneID

1040319.

GenomeReviews

Gene locus c3958 in contig AE014075_GR.

KEGG

ecc:c3958.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P67653.

OMA

P67653. KTFNTLD.

Enzyme and pathway databases

BRENDA

3.1.3.45. 292881.

Family and domain databases

InterPro

IPR013200. HAD-SF_hydro-like_3.
IPR006549. HAD-SF_hydro_IIIA.
IPR010023. Phosphatase_KdsC.
[Graphical view]

Pfam

PF08282. Hydrolase_3. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01662. HAD-SF-IIIA. 1 hit.
TIGR01670. YrbI-phosphatas. 1 hit.

ProtoNet

Search...

Entry information

Entry name

KDSC_ECOL6

Accession

Primary (citable) accession number: P67653
Secondary accession number(s): Q83JF3, Q8FD72

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)