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Protein

Lysine--tRNA ligase 1

Gene

lysS1

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi415 – 4151Magnesium 1By similarity
Metal bindingi422 – 4221Magnesium 1By similarity
Metal bindingi422 – 4221Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase 1 (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase 1
Short name:
LysRS 1
Gene namesi
Name:lysS1
Synonyms:lysS
Ordered Locus Names:Mb3629c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Lysine--tRNA ligase 1PRO_0000152654Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP67608.
SMRiP67608. Positions 9-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000236578.
KOiK04567.
OMAiDMMNLTE.
OrthoDBiEOG69PQ2M.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P67608-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAADTAEDL PEQFRIRRDK RARLLAQGRD PYPVAVPRTH TLAEVRAAHP
60 70 80 90 100
DLPIDTATED IVGVAGRVIF ARNSGKLCFA TLQDGDGTQL QVMISLDKVG
110 120 130 140 150
QAALDAWKAD VDLGDIVYVH GAVISSRRGE LSVLADCWRI AAKSLRPLPV
160 170 180 190 200
AHKEMSEESR VRQRYVDLIV RPEARAVARL RIAVVRAIRT ALQRRGFLEV
210 220 230 240 250
ETPVLQTLAG GAAARPFATH SNALDIDLYL RIAPELFLKR CIVGGFDKVF
260 270 280 290 300
ELNRVFRNEG ADSTHSPEFS MLETYQTYGT YDDSAVVTRE LIQEVADEAI
310 320 330 340 350
GTRQLPLPDG SVYDIDGEWA TIQMYPSLSV ALGEEITPQT TVDRLRGIAD
360 370 380 390 400
SLGLEKDPAI HDNRGFGHGK LIEELWERTV GKSLSAPTFV KDFPVQTTPL
410 420 430 440 450
TRQHRSIPGV TEKWDLYLRG IELATGYSEL SDPVVQRERF ADQARAAAAG
460 470 480 490 500
DDEAMVLDED FLAALEYGMP PCTGTGMGID RLLMSLTGLS IRETVLFPIV

RPHSN
Length:505
Mass (Da):55,709
Last modified:October 11, 2004 - v1
Checksum:iE12FFC867152740C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44899.1.
RefSeqiNP_857268.1. NC_002945.3.
WP_003419514.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO44899; CDO44899; Mb3629c.
GeneIDi1093140.
KEGGimbo:Mb3629c.
PATRICi18009695. VBIMycBov88188_3975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44899.1.
RefSeqiNP_857268.1. NC_002945.3.
WP_003419514.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP67608.
SMRiP67608. Positions 9-501.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO44899; CDO44899; Mb3629c.
GeneIDi1093140.
KEGGimbo:Mb3629c.
PATRICi18009695. VBIMycBov88188_3975.

Phylogenomic databases

HOGENOMiHOG000236578.
KOiK04567.
OMAiDMMNLTE.
OrthoDBiEOG69PQ2M.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiSYK1_MYCBO
AccessioniPrimary (citable) accession number: P67608
Secondary accession number(s): O06284, X2BNU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 11, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two lysyl-tRNA ligases in M.bovis.

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.