P67608 (SYK1_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase 1
EC=6.1.1.6

Alternative name(s):
Lysyl-tRNA synthetase 1
Short name=LysRS 1

Gene names

Name:	lysS1
Synonyms:	lysS
Ordered Locus Names:	Mb3629c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity. HAMAP MF_00252
Subunit structure	Homodimer By similarity. HAMAP MF_00252
Subcellular location	Cytoplasm HAMAP MF_00252.
Miscellaneous	There are two lysyl-tRNA ligases in M.bovis. HAMAP MF_00252
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW lysine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Lysine--tRNA ligase 1 HAMAP MF_00252		PRO_0000152654
Sites
	Metal binding	415	1	Magnesium 1 By similarity
	Metal binding	422	1	Magnesium 1 By similarity
	Metal binding	422	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67608 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: E12FFC867152740C	FASTA	505	55,709
	10 20 30 40 50 60 MSAADTAEDL PEQFRIRRDK RARLLAQGRD PYPVAVPRTH TLAEVRAAHP DLPIDTATED 70 80 90 100 110 120 IVGVAGRVIF ARNSGKLCFA TLQDGDGTQL QVMISLDKVG QAALDAWKAD VDLGDIVYVH 130 140 150 160 170 180 GAVISSRRGE LSVLADCWRI AAKSLRPLPV AHKEMSEESR VRQRYVDLIV RPEARAVARL 190 200 210 220 230 240 RIAVVRAIRT ALQRRGFLEV ETPVLQTLAG GAAARPFATH SNALDIDLYL RIAPELFLKR 250 260 270 280 290 300 CIVGGFDKVF ELNRVFRNEG ADSTHSPEFS MLETYQTYGT YDDSAVVTRE LIQEVADEAI 310 320 330 340 350 360 GTRQLPLPDG SVYDIDGEWA TIQMYPSLSV ALGEEITPQT TVDRLRGIAD SLGLEKDPAI 370 380 390 400 410 420 HDNRGFGHGK LIEELWERTV GKSLSAPTFV KDFPVQTTPL TRQHRSIPGV TEKWDLYLRG 430 440 450 460 470 480 IELATGYSEL SDPVVQRERF ADQARAAAAG DDEAMVLDED FLAALEYGMP PCTGTGMGID 490 500 RLLMSLTGLS IRETVLFPIV RPHSN

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248346 Genomic DNA. Translation: CAD95815.1.
RefSeq	NP_857268.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P67608.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000017163; EBMYCP00000016998; EBMYCG00000017158.
GeneID	1093140.
GenomeReviews	Gene locus Mb3629c in contig BX248333_GR.
KEGG	mbo:Mb3629c.
PATRIC	18009695. VBIMycBov88188_3975.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017004.
HOGENOM	HBG631383.
OMA	VEHKLEQ.
ProtClustDB	PRK00484.
Enzyme and pathway databases
BioCyc	MBOV233413:MB3629C-MONOMER.
Family and domain databases
HAMAP	MF_00252. Lys_tRNA_synth_class2. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR002313. Lys-tRNA-synth_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K04567.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00499. LysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK1_MYCBO

Accession

Primary (citable) accession number: P67608
Secondary accession number(s): O06284

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program