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P67598 (SYW_STRP8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:spyM18_2245
OrganismStreptococcus pyogenes serotype M18 (strain MGAS8232) [Complete proteome] [HAMAP]
Taxonomic identifier186103 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136695

Regions

Motif12 – 209"HIGH" region HAMAP-Rule MF_00140
Motif202 – 2065"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2051ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P67598 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 5518E2E1E0757157

FASTA34038,373
        10         20         30         40         50         60 
MTKPIILTGD RPTGKLHLGH YVGSLKNRVF LQNENKYKMF VFLADQQALT DHAKESELIQ 

        70         80         90        100        110        120 
ESIGNVALDY LSVGLDPKQS TIFIQSQIPE LAELSMYYMN LVSLARLERN PTVKTEIAQK 

       130        140        150        160        170        180 
GFGESIPSGF LVYPVSQAAD ITAFKANLVP VGNDQKPMIE QTREIVRSFN HTYHTDCLVE 

       190        200        210        220        230        240 
PEGIYPENEK AGRLPGLDGN AKMSKSLGNG IYLSDDADTV RKKVMSMYTD PNHIKIEDPG 

       250        260        270        280        290        300 
QIEGNMVFHY LDIFARKEDQ ADIEAMKEHY QRGGLGDVKT KRYLLDILER ELAPIRERRL 

       310        320        330        340 
EYAKDMGEVF RMLQEGSQKA RTVAAKTLSE VKSAMGINYF 

« Hide

References

[1]"Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks."
Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M.
Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MGAS8232.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009949 Genomic DNA. Translation: AAL98674.1.
RefSeqNP_608175.1. NC_003485.1.

3D structure databases

ProteinModelPortalP67598.
SMRP67598. Positions 2-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING186103.spyM18_2245.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL98674; AAL98674; spyM18_2245.
GeneID994170.
KEGGspm:spyM18_2245.
PATRIC19750869. VBIStrPyo4396_1998.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059941.
KOK01867.
OMAYGYGHAK.
OrthoDBEOG686NJQ.
ProtClustDBPRK12282.

Enzyme and pathway databases

BioCycSPYO186103:GHJG-1914-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_STRP8
AccessionPrimary (citable) accession number: P67598
Secondary accession number(s): Q8NYZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries